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The map label for this gene is benM [H]

Identifier: 85374977

GI number: 85374977

Start: 2178842

End: 2179891

Strand: Reverse

Name: benM [H]

Synonym: ELI_10750

Alternate gene names: 85374977

Gene position: 2179891-2178842 (Counterclockwise)

Preceding gene: 85374978

Following gene: 85374961

Centisome position: 71.42

GC content: 56.67

Gene sequence:

>1050_bases
TTGATCCAGGCCGTTTCGCCGCTCCAGATTTCGAGACGGGTTGCAGTCGTGCTGGGCGGCAGTGTCGAAAGTAAGATTGG
CAAAATGACCTTGATTCGCCTAATTCATTCTCTGGAGCAATTATTCCTGTGGGGCATGTCCATGCTCGATTCTCGCCTCA
AGCATGCCGTCGCGGTTGGTCAGCTTCGTTCCTTCTCCAGGGCAGCGGATGCGGTTGGTGTCACGCAGTCGGCGGTGACC
AAGAGTGTCGCTGAACTCGAACGGTATCTTGGCTATTCCCTTTTCCATCGGACCTCACGGGGCGCGATGCCGACAGAGGA
GGGACGCGAATTTATCGATCGCGCAGCGCGCTTGCTTGCCGACGCAGCTGAACTACTCGGAGATACAGCCCGCCGCGCCG
ACGCCTATGCGGGCCCGCTTCGAATCGGACTGTTCCCAGGGTCAATCGACTGGTTGCTCACCCAACCTCTGGTTTCTTTG
CTGCGGCGCCATCCGGCAGTGCGCGTCGAAGTCGTTTCGGGCAACAGCGAAAGGGGCGTGCGACTCCTGTCGCGCGGCGA
TATCGATGTCGCCTTTGGACTTGAAGCGGCCTTTGCACGCTGGCCGGAATTCAAGTGCGAGCGGGTCGCCTCGATCGAAA
TACTGCCTTTTGTGCGGAACAATCATCCCATTCTGGGGATAAACCCGACCAGCAAGGAGCCGCTGGTCCAGTTCGAATTC
GTAGTGCCCTCATCTTCCGAACCCTACACATCGATCATTCAGCAGATGTACGAAAAAAGCGGTAAGCGTCCCGCTGACTG
GATCCACATGACAGACTATTTTCAATTGGTTCGACGGATCGTTGCCACATCCGATGCTATCGGGATGGTTGCAAAACAGT
TCACCGAGAACTCATGGTTTCGCGCCAATTTCGTGGCCTTGGAGGGAATCGGTTTATTCGATCCATTGACGCTTTGTTAT
GCTGTGCGTAGCCGCTGGCAGGTAAAACCGGCTGGCCGTGCGCTCGTCAGCCTTGTTCGTCAGGCATGGCGCACCGCACC
ATCGGATTAA

Upstream 100 bases:

>100_bases
AACGGCGCCGGGCCGATGCCCGCAATTTCGAGTATTTTGAGGCCTGCAAGCGGACCAGTTTTCGTCATTCAGGCATACCT
GCGTCTGAGACCTTGGCGCT

Downstream 100 bases:

>100_bases
CGGCAAGGGGGCGGGGTGCGATCGGAAATCCGCTGCATCCGTCGCGATCAGCTAGAGCATTTCGCGCAGAAGTGGGAACC
GGCTTTCCGGCAGGAAATGC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIQAVSPLQISRRVAVVLGGSVESKIGKMTLIRLIHSLEQLFLWGMSMLDSRLKHAVAVGQLRSFSRAADAVGVTQSAVT
KSVAELERYLGYSLFHRTSRGAMPTEEGREFIDRAARLLADAAELLGDTARRADAYAGPLRIGLFPGSIDWLLTQPLVSL
LRRHPAVRVEVVSGNSERGVRLLSRGDIDVAFGLEAAFARWPEFKCERVASIEILPFVRNNHPILGINPTSKEPLVQFEF
VVPSSSEPYTSIIQQMYEKSGKRPADWIHMTDYFQLVRRIVATSDAIGMVAKQFTENSWFRANFVALEGIGLFDPLTLCY
AVRSRWQVKPAGRALVSLVRQAWRTAPSD

Sequences:

>Translated_349_residues
MIQAVSPLQISRRVAVVLGGSVESKIGKMTLIRLIHSLEQLFLWGMSMLDSRLKHAVAVGQLRSFSRAADAVGVTQSAVT
KSVAELERYLGYSLFHRTSRGAMPTEEGREFIDRAARLLADAAELLGDTARRADAYAGPLRIGLFPGSIDWLLTQPLVSL
LRRHPAVRVEVVSGNSERGVRLLSRGDIDVAFGLEAAFARWPEFKCERVASIEILPFVRNNHPILGINPTSKEPLVQFEF
VVPSSSEPYTSIIQQMYEKSGKRPADWIHMTDYFQLVRRIVATSDAIGMVAKQFTENSWFRANFVALEGIGLFDPLTLCY
AVRSRWQVKPAGRALVSLVRQAWRTAPSD
>Mature_349_residues
MIQAVSPLQISRRVAVVLGGSVESKIGKMTLIRLIHSLEQLFLWGMSMLDSRLKHAVAVGQLRSFSRAADAVGVTQSAVT
KSVAELERYLGYSLFHRTSRGAMPTEEGREFIDRAARLLADAAELLGDTARRADAYAGPLRIGLFPGSIDWLLTQPLVSL
LRRHPAVRVEVVSGNSERGVRLLSRGDIDVAFGLEAAFARWPEFKCERVASIEILPFVRNNHPILGINPTSKEPLVQFEF
VVPSSSEPYTSIIQQMYEKSGKRPADWIHMTDYFQLVRRIVATSDAIGMVAKQFTENSWFRANFVALEGIGLFDPLTLCY
AVRSRWQVKPAGRALVSLVRQAWRTAPSD

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787601, Length=238, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=294, Percent_Identity=24.8299319727891, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693193, Length=130, Percent_Identity=35.3846153846154, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081904, Length=281, Percent_Identity=25.2669039145907, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788748, Length=160, Percent_Identity=31.25, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790399, Length=138, Percent_Identity=28.9855072463768, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786401, Length=138, Percent_Identity=36.231884057971, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38837; Mature: 38837

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQAVSPLQISRRVAVVLGGSVESKIGKMTLIRLIHSLEQLFLWGMSMLDSRLKHAVAVG
CCCCCCCHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLRSFSRAADAVGVTQSAVTKSVAELERYLGYSLFHRTSRGAMPTEEGREFIDRAARLLA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DAAELLGDTARRADAYAGPLRIGLFPGSIDWLLTQPLVSLLRRHPAVRVEVVSGNSERGV
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
RLLSRGDIDVAFGLEAAFARWPEFKCERVASIEILPFVRNNHPILGINPTSKEPLVQFEF
EEEECCCEEEEECHHHHHHHCCCHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCEEEEE
VVPSSSEPYTSIIQQMYEKSGKRPADWIHMTDYFQLVRRIVATSDAIGMVAKQFTENSWF
EECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
RANFVALEGIGLFDPLTLCYAVRSRWQVKPAGRALVSLVRQAWRTAPSD
EEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQAVSPLQISRRVAVVLGGSVESKIGKMTLIRLIHSLEQLFLWGMSMLDSRLKHAVAVG
CCCCCCCHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLRSFSRAADAVGVTQSAVTKSVAELERYLGYSLFHRTSRGAMPTEEGREFIDRAARLLA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DAAELLGDTARRADAYAGPLRIGLFPGSIDWLLTQPLVSLLRRHPAVRVEVVSGNSERGV
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
RLLSRGDIDVAFGLEAAFARWPEFKCERVASIEILPFVRNNHPILGINPTSKEPLVQFEF
EEEECCCEEEEECHHHHHHHCCCHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCEEEEE
VVPSSSEPYTSIIQQMYEKSGKRPADWIHMTDYFQLVRRIVATSDAIGMVAKQFTENSWF
EECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
RANFVALEGIGLFDPLTLCYAVRSRWQVKPAGRALVSLVRQAWRTAPSD
EEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]