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The map label for this gene is menE [H]

Identifier: 85374969

GI number: 85374969

Start: 2170533

End: 2171999

Strand: Direct

Name: menE [H]

Synonym: ELI_10710

Alternate gene names: 85374969

Gene position: 2170533-2171999 (Clockwise)

Preceding gene: 85374968

Following gene: 85374970

Centisome position: 71.11

GC content: 58.08

Gene sequence:

>1467_bases
TTGACTTTCTCGGAAGCCTGGTTGCGCGGCGTGAAGCTCGCAAATGCTCTTGCGGAGTGCGGCCTTCAACCGGGTGACCG
GATTGCTACGCTGGAGAAGAACTCCATCGAGGCCGCCGACATCATACTGGCGGCTGCCATTGGAAACTATACGCGTGTTC
CTCTCTATGCGCGCAACCGATGTGAAGCGCACGCCCATATGATCGCGAGTACCGGATCTCGGTTGGCGCTGGTCGATGAG
GCATTGGCTTCGGAGTTGGCGGGGCTGGATGCCCAAGCTCCGACGCTTGAGCGGATCATTATCCGCGATCACAACTATGA
GAATTGGTTGGCGACAGCCTCGGACCGGGATCCCGATCCCGTCGTCGCGCCGGAGGACTATTGTGTCATCCGCCACACCG
GCGGCACGACGGGCAAACCCAAGGGCGTTGCCTATCGGCACCGTTCATGGATGACGATCTCCGCCGCATTTTTCAGCATC
GGCCCCCAAGTGGCTCCAGGGGATGCCATTCTGCATGTCGGCCCGTTGTCCCATGCATCGGGCTTCCTGTTCGTGCCGGC
CTGGTATTGCGGCGCCCGCAACGTGATGATGGACGGCTTCGATCCAGCGTCCTTTCTTGACACTCTCGAGCAGGAACGGA
TCAGCCATGCCTTTGTAGCCCCCACCATGCTCAATGCCATAGTGCATCACGACGGGGCATATGGCCGCCACTTTCCGAAT
TTGAAGTTTCTGCTGAGTGCTTCAGCGCCGATCTCCGAGCCGACGCTTCGCAAGTCGTGCCAGATATTCGGGAATCATGT
TCTTCATTCGGGCTATGGACAGACCGAAATCCTGCCCATCGCGTCTATGGGGCCCAACGAGTGGTTCGGCGAATTTGAAG
GTTCTGCCCCAATTCGGGCGGTCGGACGTCCCATGAGCGGCGCCGATATCGAAATCCGTAACGAGGACGGAAAGGTGCTG
GGACCAAACGAGCCCGGCGAGATCGTCGCACGGTTCGAAAATGGCCAGATGGAGGAATTCTGGAACGATCCGGAGGAAAC
CGCCCGGCGCATGGAAGATGGCTGGGTCAAAACCGGCGATGTCGGCATGATCGACACAAACGGATTTTTGTATCTGCTCG
ATCGCAACAACGACATGATCGTGTCAGGCGGCTTCAATATCTATCCCACCGAAATCGAAAACGTAATTGCCGACCATCCC
GGTGTACTCGAGGTCGCGGTCTTTGGCGTGCCCCATGAAAAATGGGGTGAAACGCCGCTTGCAATGGTGCGTGTCAAGCC
CGGAGCACAAATCACGGAGACCGAGATAATAGATTTGGTGCGCCAGCGTCTGGGCTCCGCAAAAAAGCCAAGCAAGGTCG
TTTTTACCGCCGAGCCACTGCCGTTGAGCAATGTCGGAAAGGTGCTTCGTTCAAAGCTTCGAGAGCCCTATTGGGCGGGC
CAGGACCGGCGCATTTCGGGGGCATGA

Upstream 100 bases:

>100_bases
GCGCCTTAGAGGAGCGCGGCTGATGGACGTCCGTACGCAAATACGTCGCGCCGCACGTTATTTCGCCCAGCAAGAGGCTC
TCGTTCACGGCCACAGGCGG

Downstream 100 bases:

>100_bases
CCGACGCAGCCATCCTAGCCACATCCTCTATTCCGGTGACCGCCTGCCGGCGCGCGGGAGACTGCAATGGATTATGAATA
CATCCTGGTTGAGCAACGCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTFSEAWLRGVKLANALAECGLQPGDRIATLEKNSIEAADIILAAAIGNYTRVPLYARNRCEAHAHMIASTGSRLALVDE
ALASELAGLDAQAPTLERIIIRDHNYENWLATASDRDPDPVVAPEDYCVIRHTGGTTGKPKGVAYRHRSWMTISAAFFSI
GPQVAPGDAILHVGPLSHASGFLFVPAWYCGARNVMMDGFDPASFLDTLEQERISHAFVAPTMLNAIVHHDGAYGRHFPN
LKFLLSASAPISEPTLRKSCQIFGNHVLHSGYGQTEILPIASMGPNEWFGEFEGSAPIRAVGRPMSGADIEIRNEDGKVL
GPNEPGEIVARFENGQMEEFWNDPEETARRMEDGWVKTGDVGMIDTNGFLYLLDRNNDMIVSGGFNIYPTEIENVIADHP
GVLEVAVFGVPHEKWGETPLAMVRVKPGAQITETEIIDLVRQRLGSAKKPSKVVFTAEPLPLSNVGKVLRSKLREPYWAG
QDRRISGA

Sequences:

>Translated_488_residues
MTFSEAWLRGVKLANALAECGLQPGDRIATLEKNSIEAADIILAAAIGNYTRVPLYARNRCEAHAHMIASTGSRLALVDE
ALASELAGLDAQAPTLERIIIRDHNYENWLATASDRDPDPVVAPEDYCVIRHTGGTTGKPKGVAYRHRSWMTISAAFFSI
GPQVAPGDAILHVGPLSHASGFLFVPAWYCGARNVMMDGFDPASFLDTLEQERISHAFVAPTMLNAIVHHDGAYGRHFPN
LKFLLSASAPISEPTLRKSCQIFGNHVLHSGYGQTEILPIASMGPNEWFGEFEGSAPIRAVGRPMSGADIEIRNEDGKVL
GPNEPGEIVARFENGQMEEFWNDPEETARRMEDGWVKTGDVGMIDTNGFLYLLDRNNDMIVSGGFNIYPTEIENVIADHP
GVLEVAVFGVPHEKWGETPLAMVRVKPGAQITETEIIDLVRQRLGSAKKPSKVVFTAEPLPLSNVGKVLRSKLREPYWAG
QDRRISGA
>Mature_487_residues
TFSEAWLRGVKLANALAECGLQPGDRIATLEKNSIEAADIILAAAIGNYTRVPLYARNRCEAHAHMIASTGSRLALVDEA
LASELAGLDAQAPTLERIIIRDHNYENWLATASDRDPDPVVAPEDYCVIRHTGGTTGKPKGVAYRHRSWMTISAAFFSIG
PQVAPGDAILHVGPLSHASGFLFVPAWYCGARNVMMDGFDPASFLDTLEQERISHAFVAPTMLNAIVHHDGAYGRHFPNL
KFLLSASAPISEPTLRKSCQIFGNHVLHSGYGQTEILPIASMGPNEWFGEFEGSAPIRAVGRPMSGADIEIRNEDGKVLG
PNEPGEIVARFENGQMEEFWNDPEETARRMEDGWVKTGDVGMIDTNGFLYLLDRNNDMIVSGGFNIYPTEIENVIADHPG
VLEVAVFGVPHEKWGETPLAMVRVKPGAQITETEIIDLVRQRLGSAKKPSKVVFTAEPLPLSNVGKVLRSKLREPYWAGQ
DRRISGA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=526, Percent_Identity=27.5665399239544, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI187761343, Length=526, Percent_Identity=27.5665399239544, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI42544132, Length=504, Percent_Identity=27.3809523809524, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI38505220, Length=500, Percent_Identity=25.8, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI122937307, Length=495, Percent_Identity=26.4646464646465, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI156151445, Length=362, Percent_Identity=29.0055248618785, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI115511026, Length=499, Percent_Identity=25.250501002004, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI58082049, Length=489, Percent_Identity=24.1308793456033, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI157311624, Length=489, Percent_Identity=23.9263803680982, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI157311622, Length=489, Percent_Identity=23.9263803680982, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI28416953, Length=519, Percent_Identity=24.8554913294798, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI13375727, Length=518, Percent_Identity=25.2895752895753, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI195947378, Length=399, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI8923896, Length=551, Percent_Identity=23.4119782214156, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI253970489, Length=379, Percent_Identity=24.802110817942, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1788107, Length=522, Percent_Identity=28.1609195402299, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI145693145, Length=491, Percent_Identity=27.4949083503055, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI221142682, Length=483, Percent_Identity=25.4658385093168, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790505, Length=516, Percent_Identity=24.8062015503876, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788595, Length=363, Percent_Identity=27.8236914600551, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786529, Length=384, Percent_Identity=27.6041666666667, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786810, Length=180, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=518, Percent_Identity=25.0965250965251, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=518, Percent_Identity=25.0965250965251, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=518, Percent_Identity=25.0965250965251, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=363, Percent_Identity=31.129476584022, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=379, Percent_Identity=27.4406332453826, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17559526, Length=369, Percent_Identity=27.10027100271, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=489, Percent_Identity=26.3803680981595, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=498, Percent_Identity=25.9036144578313, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=380, Percent_Identity=26.5789473684211, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=498, Percent_Identity=26.3052208835341, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=488, Percent_Identity=25.2049180327869, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71985884, Length=374, Percent_Identity=28.8770053475936, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=522, Percent_Identity=23.7547892720307, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17564090, Length=431, Percent_Identity=24.3619489559165, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=375, Percent_Identity=25.0666666666667, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=375, Percent_Identity=25.0666666666667, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=463, Percent_Identity=23.110151187905, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=392, Percent_Identity=27.5510204081633, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323182, Length=544, Percent_Identity=23.3455882352941, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=393, Percent_Identity=22.9007633587786, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355181, Length=483, Percent_Identity=27.7432712215321, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18859661, Length=360, Percent_Identity=29.4444444444444, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24653035, Length=507, Percent_Identity=28.5996055226824, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21358303, Length=463, Percent_Identity=27.8617710583153, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI161076582, Length=508, Percent_Identity=28.3464566929134, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24656500, Length=495, Percent_Identity=26.6666666666667, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=24.4444444444444, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21356441, Length=465, Percent_Identity=26.0215053763441, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24581924, Length=511, Percent_Identity=25.6360078277886, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648260, Length=493, Percent_Identity=25.5578093306288, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20130357, Length=352, Percent_Identity=28.4090909090909, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21356947, Length=362, Percent_Identity=28.7292817679558, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648255, Length=463, Percent_Identity=24.8380129589633, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI281365686, Length=498, Percent_Identity=24.6987951807229, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648253, Length=463, Percent_Identity=24.8380129589633, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24648257, Length=354, Percent_Identity=29.0960451977401, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI62472339, Length=539, Percent_Identity=24.30426716141, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24667955, Length=539, Percent_Identity=24.30426716141, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24667959, Length=454, Percent_Identity=25.5506607929515, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21355225, Length=544, Percent_Identity=24.4485294117647, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI221329755, Length=167, Percent_Identity=29.940119760479, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53358; Mature: 53227

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSEAWLRGVKLANALAECGLQPGDRIATLEKNSIEAADIILAAAIGNYTRVPLYARNR
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEECCEEECCC
CEAHAHMIASTGSRLALVDEALASELAGLDAQAPTLERIIIRDHNYENWLATASDRDPDP
HHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCC
VVAPEDYCVIRHTGGTTGKPKGVAYRHRSWMTISAAFFSIGPQVAPGDAILHVGPLSHAS
CCCCCCEEEEEECCCCCCCCCCCEEECCCEEEEEEHHHHCCCCCCCCCEEEEECCCCCCC
GFLFVPAWYCGARNVMMDGFDPASFLDTLEQERISHAFVAPTMLNAIVHHDGAYGRHFPN
CEEEEEEHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LKFLLSASAPISEPTLRKSCQIFGNHVLHSGYGQTEILPIASMGPNEWFGEFEGSAPIRA
EEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCHHCCCCCCCCCHHH
VGRPMSGADIEIRNEDGKVLGPNEPGEIVARFENGQMEEFWNDPEETARRMEDGWVKTGD
HCCCCCCCEEEEECCCCCEECCCCCCEEEEEECCCCHHHHCCCHHHHHHHHHCCCEEECC
VGMIDTNGFLYLLDRNNDMIVSGGFNIYPTEIENVIADHPGVLEVAVFGVPHEKWGETPL
EEEEECCCEEEEEECCCCEEEECCCEECHHHHHHHHHCCCCEEEEEEECCCHHHHCCCCE
AMVRVKPGAQITETEIIDLVRQRLGSAKKPSKVVFTAEPLPLSNVGKVLRSKLREPYWAG
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCC
QDRRISGA
CCCCCCCC
>Mature Secondary Structure 
TFSEAWLRGVKLANALAECGLQPGDRIATLEKNSIEAADIILAAAIGNYTRVPLYARNR
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEECCEEECCC
CEAHAHMIASTGSRLALVDEALASELAGLDAQAPTLERIIIRDHNYENWLATASDRDPDP
HHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCC
VVAPEDYCVIRHTGGTTGKPKGVAYRHRSWMTISAAFFSIGPQVAPGDAILHVGPLSHAS
CCCCCCEEEEEECCCCCCCCCCCEEECCCEEEEEEHHHHCCCCCCCCCEEEEECCCCCCC
GFLFVPAWYCGARNVMMDGFDPASFLDTLEQERISHAFVAPTMLNAIVHHDGAYGRHFPN
CEEEEEEHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LKFLLSASAPISEPTLRKSCQIFGNHVLHSGYGQTEILPIASMGPNEWFGEFEGSAPIRA
EEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCHHCCCCCCCCCHHH
VGRPMSGADIEIRNEDGKVLGPNEPGEIVARFENGQMEEFWNDPEETARRMEDGWVKTGD
HCCCCCCCEEEEECCCCCEECCCCCCEEEEEECCCCHHHHCCCHHHHHHHHHCCCEEECC
VGMIDTNGFLYLLDRNNDMIVSGGFNIYPTEIENVIADHPGVLEVAVFGVPHEKWGETPL
EEEEECCCEEEEEECCCCEEEECCCEECHHHHHHHHHCCCCEEEEEEECCCHHHHCCCCE
AMVRVKPGAQITETEIIDLVRQRLGSAKKPSKVVFTAEPLPLSNVGKVLRSKLREPYWAG
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCC
QDRRISGA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA