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The map label for this gene is alkJ [H]

Identifier: 85374968

GI number: 85374968

Start: 2168748

End: 2170325

Strand: Direct

Name: alkJ [H]

Synonym: ELI_10705

Alternate gene names: 85374968

Gene position: 2168748-2170325 (Clockwise)

Preceding gene: 85374967

Following gene: 85374969

Centisome position: 71.05

GC content: 57.79

Gene sequence:

>1578_bases
TTGTCCGAGGACCCCGATGTCACCGTTTGCCTGCTCGAGGCAGGCGGCCCAGGCACCTCGCCGCTTGTGTCCACGCCCGG
CGCTTTTGCGGCGCTGATCCAAGACTATCGGATCAATACGCTCAACTGGCGGTTCAACACCGACCCTTCAAAGGCGCTCA
ACGACCGCCGCCTCTACAATCCGCGCGGGAAGATGCTGGGTGGATCGAGCGGTATGAACGGGATGGTCTATATCCGCGGC
GACCGGTCGGATTTCGACCACTGGGCCGAACTCGGCAACGACGGCTGGGGTTACAACGATGTCCTGCCCTATTTCCGCAA
GGCTGAGAATAATGAGCGCGGTGAGGATGAGTTTCACGGCTCGTCGGGACCGCTTCACGTATCCAACGGCAAGCGCGAAT
TCGATGTCTATGATGCTTTCATCGAGGCGGCGACTGGACTGGACCATCAAGCCAATCCGGACTTCAATGGTGCCAGCCAG
GAAGGTGTCGGCATCTATCAGTTTACCGTGAAGGACGGGAAGCGCGCCAGCGTGAAGGCGTGTTACCTTGATCCGGTGAT
GGGCAGGCGCGGCAACCTCCGCGTCGAAGTACATGCCCGCGTCCATCGTATCAGGTTTGAGGGCAACCGCGCGGTGGCAG
TCGAGTATTCCCAGGACGGGCAGTTGAAGACGATCCCGTGCGAAAAGGAAGTCATTGTCAGCGGCGGCGCCTATAATTCG
CCCCAACTGTTGATGCTTTCCGGTATCGGACCGCGTGATGAGTTGGAGAAGCATGGAATCGAAGTGATTCATGATATTCC
CGGTGTCGGCCAGAATCTTCACGACCATCCTGACCTGATGCTGAGCTACCAGTCGAAGAAGCGGCTCGGGATCGCACTCA
ATGTCGTTGGCGCGCTCAAGACCGTGCGAGACCTATTCCAGTATCTCACGCAAAGGAAAAGCTGGCTGGCATCGCCGCCC
ACGGCTGCAGGCGGTTTCTTGCGATCCGCGCCGGGGCAGAACCGGGCGGATTGCCAGGTCCATGTCGTGCCGCTCGCCTA
TCGCGACCATGCGCGCGACTACAAGCTGATGACGAAATGGGGCTATACGATCATTCTCAATATTGGGCGCCCAAAGAGCC
GCGGCTGGGTTGCCTTACATGACTCAAACCCCGAATCCGATCCCAAGATGGACCTCAATCTCTTGAGTCATCCCGACGAC
CTCAAGACGTTGCGCAATGCCTTCCGTGTCGTGCAGGAGATCCTGCATTCGGATCGCATGAAGGCGATGATGAAACGGCC
TCTCTATCCTGACCGCTACCTTGAAACTGATGAAGAGATCGACGCCTATATCCGGGCAGAAGCCAATCATGCTTATCATC
CGGTGGGAACATGCAAGATGGGCACCGACGAGATGGCGGTGGTCGACAACCGCCTGCGCGTTCACGGCCTCGCAAATATC
CGCGTGGCCGATGCCTCGATCATGCCATCAGTCGTCAACGGCAACACCAATGCAACCTGCATCATGATCGGCGAGAAAGC
CGCCGACATGATCCGGCACGATAATATGAGCAGCAAGAATAGCATCGCAGAATATTGA

Upstream 100 bases:

>100_bases
TGACGCCGGGTCGCCAGCATGGGTCGAATGACAAGGGAAGCAAATGGCTGACTATGTAATTATTGGAGGTGGGTCGTCGG
GCGGCGTTATCGCCAGCCGG

Downstream 100 bases:

>100_bases
ATTGGACGATTGGATCAATGCTGCAGACACAGATGCTTACGAAAAGAAGTCCGGAGAGGCCTATCGCATGTCAGTTTATT
ATGTGTCGACTGCGCCATTG

Product: oxidoreductase, GMC family protein

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSEDPDVTVCLLEAGGPGTSPLVSTPGAFAALIQDYRINTLNWRFNTDPSKALNDRRLYNPRGKMLGGSSGMNGMVYIRG
DRSDFDHWAELGNDGWGYNDVLPYFRKAENNERGEDEFHGSSGPLHVSNGKREFDVYDAFIEAATGLDHQANPDFNGASQ
EGVGIYQFTVKDGKRASVKACYLDPVMGRRGNLRVEVHARVHRIRFEGNRAVAVEYSQDGQLKTIPCEKEVIVSGGAYNS
PQLLMLSGIGPRDELEKHGIEVIHDIPGVGQNLHDHPDLMLSYQSKKRLGIALNVVGALKTVRDLFQYLTQRKSWLASPP
TAAGGFLRSAPGQNRADCQVHVVPLAYRDHARDYKLMTKWGYTIILNIGRPKSRGWVALHDSNPESDPKMDLNLLSHPDD
LKTLRNAFRVVQEILHSDRMKAMMKRPLYPDRYLETDEEIDAYIRAEANHAYHPVGTCKMGTDEMAVVDNRLRVHGLANI
RVADASIMPSVVNGNTNATCIMIGEKAADMIRHDNMSSKNSIAEY

Sequences:

>Translated_525_residues
MSEDPDVTVCLLEAGGPGTSPLVSTPGAFAALIQDYRINTLNWRFNTDPSKALNDRRLYNPRGKMLGGSSGMNGMVYIRG
DRSDFDHWAELGNDGWGYNDVLPYFRKAENNERGEDEFHGSSGPLHVSNGKREFDVYDAFIEAATGLDHQANPDFNGASQ
EGVGIYQFTVKDGKRASVKACYLDPVMGRRGNLRVEVHARVHRIRFEGNRAVAVEYSQDGQLKTIPCEKEVIVSGGAYNS
PQLLMLSGIGPRDELEKHGIEVIHDIPGVGQNLHDHPDLMLSYQSKKRLGIALNVVGALKTVRDLFQYLTQRKSWLASPP
TAAGGFLRSAPGQNRADCQVHVVPLAYRDHARDYKLMTKWGYTIILNIGRPKSRGWVALHDSNPESDPKMDLNLLSHPDD
LKTLRNAFRVVQEILHSDRMKAMMKRPLYPDRYLETDEEIDAYIRAEANHAYHPVGTCKMGTDEMAVVDNRLRVHGLANI
RVADASIMPSVVNGNTNATCIMIGEKAADMIRHDNMSSKNSIAEY
>Mature_524_residues
SEDPDVTVCLLEAGGPGTSPLVSTPGAFAALIQDYRINTLNWRFNTDPSKALNDRRLYNPRGKMLGGSSGMNGMVYIRGD
RSDFDHWAELGNDGWGYNDVLPYFRKAENNERGEDEFHGSSGPLHVSNGKREFDVYDAFIEAATGLDHQANPDFNGASQE
GVGIYQFTVKDGKRASVKACYLDPVMGRRGNLRVEVHARVHRIRFEGNRAVAVEYSQDGQLKTIPCEKEVIVSGGAYNSP
QLLMLSGIGPRDELEKHGIEVIHDIPGVGQNLHDHPDLMLSYQSKKRLGIALNVVGALKTVRDLFQYLTQRKSWLASPPT
AAGGFLRSAPGQNRADCQVHVVPLAYRDHARDYKLMTKWGYTIILNIGRPKSRGWVALHDSNPESDPKMDLNLLSHPDDL
KTLRNAFRVVQEILHSDRMKAMMKRPLYPDRYLETDEEIDAYIRAEANHAYHPVGTCKMGTDEMAVVDNRLRVHGLANIR
VADASIMPSVVNGNTNATCIMIGEKAADMIRHDNMSSKNSIAEY

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=518, Percent_Identity=41.5057915057915, Blast_Score=379, Evalue=1e-105,
Organism=Escherichia coli, GI1786503, Length=531, Percent_Identity=39.924670433145, Blast_Score=352, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17532301, Length=532, Percent_Identity=33.8345864661654, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24642042, Length=557, Percent_Identity=36.4452423698384, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI45551458, Length=559, Percent_Identity=36.4937388193202, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI45549471, Length=559, Percent_Identity=36.4937388193202, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24642048, Length=561, Percent_Identity=34.4028520499109, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24642055, Length=557, Percent_Identity=32.4955116696589, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24642039, Length=554, Percent_Identity=34.6570397111913, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24642037, Length=556, Percent_Identity=33.4532374100719, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642059, Length=567, Percent_Identity=33.6860670194003, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24642051, Length=551, Percent_Identity=33.3938294010889, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI18859995, Length=559, Percent_Identity=35.0626118067979, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24642035, Length=564, Percent_Identity=32.9787234042553, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17137792, Length=541, Percent_Identity=32.7171903881701, Blast_Score=256, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24650267, Length=558, Percent_Identity=32.9749103942652, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI18859993, Length=561, Percent_Identity=34.0463458110517, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24645930, Length=571, Percent_Identity=27.84588441331, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24642057, Length=486, Percent_Identity=30.6584362139918, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58447; Mature: 58316

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDPDVTVCLLEAGGPGTSPLVSTPGAFAALIQDYRINTLNWRFNTDPSKALNDRRLYN
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCEEEEEEEEECCCHHHHCCCCEEEC
PRGKMLGGSSGMNGMVYIRGDRSDFDHWAELGNDGWGYNDVLPYFRKAENNERGEDEFHG
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHCCC
SSGPLHVSNGKREFDVYDAFIEAATGLDHQANPDFNGASQEGVGIYQFTVKDGKRASVKA
CCCCEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEE
CYLDPVMGRRGNLRVEVHARVHRIRFEGNRAVAVEYSQDGQLKTIPCEKEVIVSGGAYNS
EEECHHHCCCCCEEEEEEEEEEEEEECCCEEEEEEECCCCCEEECCCCCEEEEECCCCCC
PQLLMLSGIGPRDELEKHGIEVIHDIPGVGQNLHDHPDLMLSYQSKKRLGIALNVVGALK
CCEEEEECCCCHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEHHHHHHH
TVRDLFQYLTQRKSWLASPPTAAGGFLRSAPGQNRADCQVHVVPLAYRDHARDYKLMTKW
HHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEC
GYTIILNIGRPKSRGWVALHDSNPESDPKMDLNLLSHPDDLKTLRNAFRVVQEILHSDRM
CEEEEEECCCCCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
KAMMKRPLYPDRYLETDEEIDAYIRAEANHAYHPVGTCKMGTDEMAVVDNRLRVHGLANI
HHHHHCCCCCCHHCCCCHHHCEEEEECCCCCCCCCCEEECCCCCEEECCCCEEEEEEEEE
RVADASIMPSVVNGNTNATCIMIGEKAADMIRHDNMSSKNSIAEY
EEECCHHCCCEECCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SEDPDVTVCLLEAGGPGTSPLVSTPGAFAALIQDYRINTLNWRFNTDPSKALNDRRLYN
CCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCEEEEEEEEECCCHHHHCCCCEEEC
PRGKMLGGSSGMNGMVYIRGDRSDFDHWAELGNDGWGYNDVLPYFRKAENNERGEDEFHG
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHCCC
SSGPLHVSNGKREFDVYDAFIEAATGLDHQANPDFNGASQEGVGIYQFTVKDGKRASVKA
CCCCEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEE
CYLDPVMGRRGNLRVEVHARVHRIRFEGNRAVAVEYSQDGQLKTIPCEKEVIVSGGAYNS
EEECHHHCCCCCEEEEEEEEEEEEEECCCEEEEEEECCCCCEEECCCCCEEEEECCCCCC
PQLLMLSGIGPRDELEKHGIEVIHDIPGVGQNLHDHPDLMLSYQSKKRLGIALNVVGALK
CCEEEEECCCCHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEHHHHHHH
TVRDLFQYLTQRKSWLASPPTAAGGFLRSAPGQNRADCQVHVVPLAYRDHARDYKLMTKW
HHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEC
GYTIILNIGRPKSRGWVALHDSNPESDPKMDLNLLSHPDDLKTLRNAFRVVQEILHSDRM
CEEEEEECCCCCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
KAMMKRPLYPDRYLETDEEIDAYIRAEANHAYHPVGTCKMGTDEMAVVDNRLRVHGLANI
HHHHHCCCCCCHHCCCCHHHCEEEEECCCCCCCCCCEEECCCCCEEECCCCEEEEEEEEE
RVADASIMPSVVNGNTNATCIMIGEKAADMIRHDNMSSKNSIAEY
EEECCHHCCCEECCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]