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The map label for this gene is cobB [H]

Identifier: 85374943

GI number: 85374943

Start: 2145284

End: 2145985

Strand: Reverse

Name: cobB [H]

Synonym: ELI_10580

Alternate gene names: 85374943

Gene position: 2145985-2145284 (Counterclockwise)

Preceding gene: 85374946

Following gene: 85374942

Centisome position: 70.3

GC content: 65.67

Gene sequence:

>702_bases
TTGACCCAAGAAATCCGCAACATCGTCATCCTTACCGGGGCCGGGATATCCGCAGAGAGCGGCATCGATACCTTCCGCAG
CGCAGGGGGATTGTGGGAGCAGCACAATGTCGAGGACGTCGCGACGCCCGAAGGCTTTACCCGCAGCCCGGAGCTGGTGC
TGAACTTCTACGACATGCGGCGCGAGGCTCTCGGAAAGGTGCAGCCCAATCCGGCGCACGACGCACTCGCGCGGCTCGAT
GCGGAGTTCGGGGGCGAGTTGCTGATCGTCACGCAGAATGTCGACGACCTGCATGAGCGGGCCGGGGCGAAGCGCGTGCT
GCACATGCATGGCGAGCTGAAGAGCGCGCTGTGCCTCGCCTGCGAGATGCGCTCGCCTTGGGATGCGACTCTGTCCGACC
GCCCGGCGTGCCCGGTATGTCAGGCGCCGAGCCTGCGCCCCGACGTGGTGTGGTTCGGCGAGATGCCCTACCAGATGGAC
CGCATCTACCGCGCCATTCGCGAGGCGGACCTGTTCGTTAGCATCGGTACCTCCGGCGCGGTCTATCCGGCGGCGGGGTT
CGTGCAGGACGCCCGCAATCTTGGCGTGCAGACGCTGGAGCTGAATTTGGAGCGCAGCGAGGGCTCGCATTGGTTTCATG
AGTCGCGACTGGGGTCGGCGGGGGAATTGGTGCCGGAATGGGTGGGGGAGGTGTTGGGGTGA

Upstream 100 bases:

>100_bases
CCGTTCGGGCTGAGCTTGTCGAAGCCCCGCACTTCCTTCATGCCCTGAGCTAGAAGAAAAGACAGCCCTTCGACAAGCTC
AGGGCGAACGGAGGTGGGGT

Downstream 100 bases:

>100_bases
GTCGTCTGGTGAAATTGGATTTGTTTGCAGACTTTTTCCAAGTCTACATTGCGGACGGGAAGTTCCGCACTGACACATCG
GACATCTGGAATAACAAAGC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTQEIRNIVILTGAGISAESGIDTFRSAGGLWEQHNVEDVATPEGFTRSPELVLNFYDMRREALGKVQPNPAHDALARLD
AEFGGELLIVTQNVDDLHERAGAKRVLHMHGELKSALCLACEMRSPWDATLSDRPACPVCQAPSLRPDVVWFGEMPYQMD
RIYRAIREADLFVSIGTSGAVYPAAGFVQDARNLGVQTLELNLERSEGSHWFHESRLGSAGELVPEWVGEVLG

Sequences:

>Translated_233_residues
MTQEIRNIVILTGAGISAESGIDTFRSAGGLWEQHNVEDVATPEGFTRSPELVLNFYDMRREALGKVQPNPAHDALARLD
AEFGGELLIVTQNVDDLHERAGAKRVLHMHGELKSALCLACEMRSPWDATLSDRPACPVCQAPSLRPDVVWFGEMPYQMD
RIYRAIREADLFVSIGTSGAVYPAAGFVQDARNLGVQTLELNLERSEGSHWFHESRLGSAGELVPEWVGEVLG
>Mature_232_residues
TQEIRNIVILTGAGISAESGIDTFRSAGGLWEQHNVEDVATPEGFTRSPELVLNFYDMRREALGKVQPNPAHDALARLDA
EFGGELLIVTQNVDDLHERAGAKRVLHMHGELKSALCLACEMRSPWDATLSDRPACPVCQAPSLRPDVVWFGEMPYQMDR
IYRAIREADLFVSIGTSGAVYPAAGFVQDARNLGVQTLELNLERSEGSHWFHESRLGSAGELVPEWVGEVLG

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=233, Percent_Identity=37.7682403433476, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI6912664, Length=251, Percent_Identity=36.6533864541833, Blast_Score=160, Evalue=8e-40,
Organism=Homo sapiens, GI13787215, Length=237, Percent_Identity=37.1308016877637, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI7657575, Length=204, Percent_Identity=33.8235294117647, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI6912662, Length=244, Percent_Identity=30.7377049180328, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI6912660, Length=224, Percent_Identity=33.4821428571429, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI63054862, Length=219, Percent_Identity=33.7899543378995, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI215982798, Length=142, Percent_Identity=34.5070422535211, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI13775602, Length=230, Percent_Identity=28.695652173913, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI13775600, Length=230, Percent_Identity=28.695652173913, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI308199517, Length=228, Percent_Identity=55.2631578947368, Blast_Score=247, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17541892, Length=200, Percent_Identity=32, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17137536, Length=224, Percent_Identity=30.3571428571429, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28571445, Length=260, Percent_Identity=28.0769230769231, Blast_Score=80, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 25656; Mature: 25525

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEIRNIVILTGAGISAESGIDTFRSAGGLWEQHNVEDVATPEGFTRSPELVLNFYDMR
CCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
REALGKVQPNPAHDALARLDAEFGGELLIVTQNVDDLHERAGAKRVLHMHGELKSALCLA
HHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CEMRSPWDATLSDRPACPVCQAPSLRPDVVWFGEMPYQMDRIYRAIREADLFVSIGTSGA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCCC
VYPAAGFVQDARNLGVQTLELNLERSEGSHWFHESRLGSAGELVPEWVGEVLG
CCCCHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TQEIRNIVILTGAGISAESGIDTFRSAGGLWEQHNVEDVATPEGFTRSPELVLNFYDMR
CCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
REALGKVQPNPAHDALARLDAEFGGELLIVTQNVDDLHERAGAKRVLHMHGELKSALCLA
HHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CEMRSPWDATLSDRPACPVCQAPSLRPDVVWFGEMPYQMDRIYRAIREADLFVSIGTSGA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCCC
VYPAAGFVQDARNLGVQTLELNLERSEGSHWFHESRLGSAGELVPEWVGEVLG
CCCCHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]