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The map label for this gene is ruvA

Identifier: 85374915

GI number: 85374915

Start: 2117842

End: 2118444

Strand: Reverse

Name: ruvA

Synonym: ELI_10440

Alternate gene names: 85374915

Gene position: 2118444-2117842 (Counterclockwise)

Preceding gene: 85374919

Following gene: 85374914

Centisome position: 69.4

GC content: 67.83

Gene sequence:

>603_bases
ATGATTGCCAAGCTGAAAGGCCTGCTCGACGAAACCGGCACCGACTGGGCTGTGATCGACGTCTCGGGCGTCGGATATCT
CGTCCATTGCTCCTCGAAATCGCTCGCCGCGTTGGGCGAAGTCGGGGAGGCTTGCACGGTCTATACCGACCTGCAGGTGA
GCGAGAACGACATGCGGTTGCTGGGCTTTGCCGAAGCGAGCGAGCGCGACTGGTTCCGCCTGCTGACGCAGGTGCAGGGC
GTCGGCAGCAAGGTCGCGCTGGCGATCCTGTCGGCGCTGTCGACCGAGGAAGTGCAGACCGCCTGCGCCAATGGCGATGC
CGCGATGGTGGCCCGCGCGCAGGGTGTCGGGCCGAAGCTCGCCGGGCGGATCGTCAACGAACTGAAGGACAAGGCCGGTG
CCTTGCCGAGCGCACCGGGCGGGGCGGCAATGGCTGCAAACCCGGCTGGAGGCGCGAGCGCGGATGCGGTCAGCGCGCTG
GAGAACCTCGGTTTCAAACCCGCCATCGCCGCCCGCGCCGTCGCCACTGCGCAGGGTGAATTGGGCGAAGGTGCGAGCGA
GAGCGAACTGATCCGCGTGGCGCTGAAGCGGGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
TGTGTTCCTCACCGTCGATGAACCGTGCAGCAGAAGGGGTGACGATAACGACTCGCTCGCTACTGGCGTTTGGCCGGCAA
ATGGGGGAGAAGCCGCAGTC

Downstream 100 bases:

>100_bases
TGGAGAGGACAATGAGAGCTGTTGTGGCAGTCGGGATGATGGCGTTGGCTTTGCCAGCGAGCGCGCAGGACATGGCGGCC
TTCAGGAGCGGACCGGTTTT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MIAKLKGLLDETGTDWAVIDVSGVGYLVHCSSKSLAALGEVGEACTVYTDLQVSENDMRLLGFAEASERDWFRLLTQVQG
VGSKVALAILSALSTEEVQTACANGDAAMVARAQGVGPKLAGRIVNELKDKAGALPSAPGGAAMAANPAGGASADAVSAL
ENLGFKPAIAARAVATAQGELGEGASESELIRVALKRAAG

Sequences:

>Translated_200_residues
MIAKLKGLLDETGTDWAVIDVSGVGYLVHCSSKSLAALGEVGEACTVYTDLQVSENDMRLLGFAEASERDWFRLLTQVQG
VGSKVALAILSALSTEEVQTACANGDAAMVARAQGVGPKLAGRIVNELKDKAGALPSAPGGAAMAANPAGGASADAVSAL
ENLGFKPAIAARAVATAQGELGEGASESELIRVALKRAAG
>Mature_200_residues
MIAKLKGLLDETGTDWAVIDVSGVGYLVHCSSKSLAALGEVGEACTVYTDLQVSENDMRLLGFAEASERDWFRLLTQVQG
VGSKVALAILSALSTEEVQTACANGDAAMVARAQGVGPKLAGRIVNELKDKAGALPSAPGGAAMAANPAGGASADAVSAL
ENLGFKPAIAARAVATAQGELGEGASESELIRVALKRAAG

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=205, Percent_Identity=35.1219512195122, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_ERYLH (Q2N811)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458977.1
- ProteinModelPortal:   Q2N811
- SMR:   Q2N811
- STRING:   Q2N811
- GeneID:   3869631
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_10440
- NMPDR:   fig|314225.3.peg.943
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LFGFAQK
- PhylomeDB:   Q2N811
- ProtClustDB:   PRK00116
- BioCyc:   ELIT314225:ELI_10440-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 20167; Mature: 20167

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKLKGLLDETGTDWAVIDVSGVGYLVHCSSKSLAALGEVGEACTVYTDLQVSENDMRL
CCCHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHHCCCEEEEEEECEECCCCEEE
LGFAEASERDWFRLLTQVQGVGSKVALAILSALSTEEVQTACANGDAAMVARAQGVGPKL
EEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHCCCCHHH
AGRIVNELKDKAGALPSAPGGAAMAANPAGGASADAVSALENLGFKPAIAARAVATAQGE
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
LGEGASESELIRVALKRAAG
CCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAKLKGLLDETGTDWAVIDVSGVGYLVHCSSKSLAALGEVGEACTVYTDLQVSENDMRL
CCCHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHHCCCEEEEEEECEECCCCEEE
LGFAEASERDWFRLLTQVQGVGSKVALAILSALSTEEVQTACANGDAAMVARAQGVGPKL
EEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHCCCCHHH
AGRIVNELKDKAGALPSAPGGAAMAANPAGGASADAVSALENLGFKPAIAARAVATAQGE
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
LGEGASESELIRVALKRAAG
CCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA