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The map label for this gene is ruvB

Identifier: 85374912

GI number: 85374912

Start: 2115817

End: 2116848

Strand: Reverse

Name: ruvB

Synonym: ELI_10425

Alternate gene names: 85374912

Gene position: 2116848-2115817 (Counterclockwise)

Preceding gene: 85374914

Following gene: 85374909

Centisome position: 69.35

GC content: 68.02

Gene sequence:

>1032_bases
GTGACCGACCCCATCCCCCTCCACACGCCCGATCGCCAGCCGGAAGACCCGGACGTGGCATTGCGGCCCAAGTCGCTGGC
CGAATTCGTCGGGCAGGCGGCGGCGAAGGACAATCTTGCTGTCTTCATCGAAGCCGCCAGGTCGCGCGGCGAGGCGATGG
ATCATACGCTGTTCTTCGGTCCGCCGGGGCTGGGCAAGACGACGCTGGCGCAGATCATCGCGCGCGAGCTGGGCGTGGGG
TTTCGCGCCACGTCGGGGCCGGTGATCGCCAAGGCGGGCGACCTCGCGGCGCTGCTGACCAATCTCGAGCCGCATGACGT
GCTCTTCATCGACGAGATTCACCGTCTAAACCCTGTGGTAGAGGAAGTGCTCTATCCGGCGATGGAGGACCGCGCGCTCG
ACCTCATCATCGGCGAAGGACCGGCGGCACGCAGCGTGCGGATCGACCTGCCGCCCTTTACCCTGATCGGCGCCACGACC
CGGCAGGGCCTGCTGACGACGCCCCTGCGCGATCGCTTCGGCATCCCGGTGCGGTTGAACTTCTATACCGAGGAGGAGTT
GGAGAAAGTCGTCACCCGCGGAGCCGGGCTGATGGGGCTCGCTATCGATGCCGCGGGCGCGCGCGAGATCGCGCGGCGCA
GCCGGGGGACGCCGCGCGTTGCCGGACGCCTGCTGCGCCGGGTGCGCGACTTTGCCGAGGTACAAAAGGCTGGAACCGTA
ACCTCACCGATCGCCGATGCCGCGCTCACGCGGCTCGAGGTCGACGGGCTCGGGCTGGATGCGATGGATCGCCGTTACCT
CACCATGATCGCGGACATCTACAAGGGCGGGCCGGTCGGTGTCGAAACGCTGGCGGCGGGCCTGGCCGAGCCGCGCGACA
CGATCGAGGACGTGGTCGAGCCATACCTGATCCAGCTCGGCCTCGTCGCCCGCACGGCGCGCGGGCGCTGCCTCAACGAT
CGCGGTTGGGAACATCTCGAACGCCAGCCGCCAGCCGGCTCGCAAACGGGCTTATTCGACGGAAAGTCTTAA

Upstream 100 bases:

>100_bases
GCAACAATATAGACGCAAGGATTTCGCTCGATCGGCATGCTTCGGTGATTGCCGCACCATGGATGCTGAAACAAGTTCAG
CATGACGATATGGGGGTGGT

Downstream 100 bases:

>100_bases
CGCCGCAGAAAGGAATTCAACGATTTCGGTGCCATAGCGGGACAAGTCGTTGGCTAACGGGGCTTTCGGCTGAAAAATGT
CGCCACGCCATGTCGCTTTC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTDPIPLHTPDRQPEDPDVALRPKSLAEFVGQAAAKDNLAVFIEAARSRGEAMDHTLFFGPPGLGKTTLAQIIARELGVG
FRATSGPVIAKAGDLAALLTNLEPHDVLFIDEIHRLNPVVEEVLYPAMEDRALDLIIGEGPAARSVRIDLPPFTLIGATT
RQGLLTTPLRDRFGIPVRLNFYTEEELEKVVTRGAGLMGLAIDAAGAREIARRSRGTPRVAGRLLRRVRDFAEVQKAGTV
TSPIADAALTRLEVDGLGLDAMDRRYLTMIADIYKGGPVGVETLAAGLAEPRDTIEDVVEPYLIQLGLVARTARGRCLND
RGWEHLERQPPAGSQTGLFDGKS

Sequences:

>Translated_343_residues
MTDPIPLHTPDRQPEDPDVALRPKSLAEFVGQAAAKDNLAVFIEAARSRGEAMDHTLFFGPPGLGKTTLAQIIARELGVG
FRATSGPVIAKAGDLAALLTNLEPHDVLFIDEIHRLNPVVEEVLYPAMEDRALDLIIGEGPAARSVRIDLPPFTLIGATT
RQGLLTTPLRDRFGIPVRLNFYTEEELEKVVTRGAGLMGLAIDAAGAREIARRSRGTPRVAGRLLRRVRDFAEVQKAGTV
TSPIADAALTRLEVDGLGLDAMDRRYLTMIADIYKGGPVGVETLAAGLAEPRDTIEDVVEPYLIQLGLVARTARGRCLND
RGWEHLERQPPAGSQTGLFDGKS
>Mature_342_residues
TDPIPLHTPDRQPEDPDVALRPKSLAEFVGQAAAKDNLAVFIEAARSRGEAMDHTLFFGPPGLGKTTLAQIIARELGVGF
RATSGPVIAKAGDLAALLTNLEPHDVLFIDEIHRLNPVVEEVLYPAMEDRALDLIIGEGPAARSVRIDLPPFTLIGATTR
QGLLTTPLRDRFGIPVRLNFYTEEELEKVVTRGAGLMGLAIDAAGAREIARRSRGTPRVAGRLLRRVRDFAEVQKAGTVT
SPIADAALTRLEVDGLGLDAMDRRYLTMIADIYKGGPVGVETLAAGLAEPRDTIEDVVEPYLIQLGLVARTARGRCLNDR
GWEHLERQPPAGSQTGLFDGKS

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=319, Percent_Identity=63.6363636363636, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787119, Length=235, Percent_Identity=30.2127659574468, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_ERYLH (Q2N814)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458974.1
- ProteinModelPortal:   Q2N814
- SMR:   Q2N814
- STRING:   Q2N814
- GeneID:   3869628
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_10425
- NMPDR:   fig|314225.3.peg.946
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q2N814
- ProtClustDB:   PRK00080
- BioCyc:   ELIT314225:ELI_10425-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37071; Mature: 36940

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPIPLHTPDRQPEDPDVALRPKSLAEFVGQAAAKDNLAVFIEAARSRGEAMDHTLFFG
CCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHCCEEEEC
PPGLGKTTLAQIIARELGVGFRATSGPVIAKAGDLAALLTNLEPHDVLFIDEIHRLNPVV
CCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDRALDLIIGEGPAARSVRIDLPPFTLIGATTRQGLLTTPLRDRFGIPVRLN
HHHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEEECCCCCCCEECCCHHHCCCCEEEE
FYTEEELEKVVTRGAGLMGLAIDAAGAREIARRSRGTPRVAGRLLRRVRDFAEVQKAGTV
ECCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
TSPIADAALTRLEVDGLGLDAMDRRYLTMIADIYKGGPVGVETLAAGLAEPRDTIEDVVE
CHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHH
PYLIQLGLVARTARGRCLNDRGWEHLERQPPAGSQTGLFDGKS
HHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDPIPLHTPDRQPEDPDVALRPKSLAEFVGQAAAKDNLAVFIEAARSRGEAMDHTLFFG
CCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHCCEEEEC
PPGLGKTTLAQIIARELGVGFRATSGPVIAKAGDLAALLTNLEPHDVLFIDEIHRLNPVV
CCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDRALDLIIGEGPAARSVRIDLPPFTLIGATTRQGLLTTPLRDRFGIPVRLN
HHHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEEECCCCCCCEECCCHHHCCCCEEEE
FYTEEELEKVVTRGAGLMGLAIDAAGAREIARRSRGTPRVAGRLLRRVRDFAEVQKAGTV
ECCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
TSPIADAALTRLEVDGLGLDAMDRRYLTMIADIYKGGPVGVETLAAGLAEPRDTIEDVVE
CHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHH
PYLIQLGLVARTARGRCLNDRGWEHLERQPPAGSQTGLFDGKS
HHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA