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The map label for this gene is gltT [H]

Identifier: 85374875

GI number: 85374875

Start: 2078935

End: 2080224

Strand: Reverse

Name: gltT [H]

Synonym: ELI_10240

Alternate gene names: 85374875

Gene position: 2080224-2078935 (Counterclockwise)

Preceding gene: 85374879

Following gene: 85374874

Centisome position: 68.15

GC content: 64.65

Gene sequence:

>1290_bases
TTGGCCGAATCTGAGACGCGCGGCGGCGCGGATGACAGCGGCGGGCTGGTCACGATCCGCCTGCCGGTGTGGTGGACATT
CGGCGGGCTGATTGCCGGCTTGCTGACGGGCCTGTTGCTGGCCGGATCGAATGTGATCGAGCCGCTGCTGGAAGTGACCG
GACCGGTCGGAACGCTGTGGCTGCGCGCCCTGCAGATGACCATCATTCCCCTGGTCGCGGCTCTCCTGGTGTTGGGCATC
ACCAAGATGGCGCAAGCCGCGCGTGCGGGGGCGACCGCGCGGCGGATGCTGTTGATGGTGTTCGGCTTGCTGGTCTTCAG
CGGGGTCGCTTCGGCGATCAGCATGCCGCTCTTGCTCGATGCCTTTCCGCCACCTTCGGGTGCTTCGGGCATTCTGGATG
CCCCCAGTGCGGAAACGCAGCAGGTGCCGACCATCGGCGACTTCATCGCTTCTCTGATTGCGCCCAATATCATCCAGGCT
GCGGCTGAGACATCGACCTTGCCGCTGACAATATTCTTTGCGCTTTTCGCCGTTGCTGTCGCGCGCCTGCCTGGGGCGCA
GCGTGCAACCATGCTGCGGTTCTTCGAAGCGCTGGCCAACACCATGCTGACGATCATCGGCTGGGTGCTGTGGGTCGCCC
CGGTGGGCGTGTTCGCCCTGGCAGTGGGCGTTGCTGCGCGTGCCGGAGGCGAGGCGTTCGCAACGCTGGCGCATTACATC
GTCACCGTAACGGCCATGGGTGCCATCGTGCTGGTGGCCGCATACGCGCTCGGGATCATAGCGGGCCGGATATCGCCAGC
GAAGTTCGCGCGTTCGGTGCTGCCTGCCCAGGCTGTGGCCATATCAACGCAAAGCTCGCTCGCCAGCCTTCCGGCCATGC
TCGACAGCGCGCGGCGGCTGGGCATTCGCGAGGAGGTCGCCGATTTCGTGCTGCCGCTGGCCGTTGCGATCTTTCGCGCG
ACAAGCCCGGCCATGAACATGGCTGTCGCGATCTACGTCGCGCATCTGGCGGGCGTCGAACTGACGCCGATGCTCCTTGC
AGCCGGCATAGCCGTGGCTTTCGTCATCAGCATAGGCTCGGTCAGCCTGCCCGGCTCGATCAGTTTCGTCATCTCGATCG
GCCCGATTGCGCTCGCCATGGGCATTCCGATCGAACCGCTTGCGCTGCTGGTCGCGGTCGAGATGCTGCCGGATATCATG
CGAACGCTGGCCAATGTGACGATGAATGTCGGACTGACTGCGGCGGTCGACCGTGAAAAATCGGTTTCCATGCAACCCGA
GAGCGCCTGA

Upstream 100 bases:

>100_bases
TGTTGCGACATTACCGGTTGCGAGAACCCTCGCGATTTGCCACGCGCCTTATCCGCTAAGCACCCGCGAAGGGAAGAGGC
GAATAGGGGAGGGCCGGGTA

Downstream 100 bases:

>100_bases
TGCGTTCATCTCACAGACACCCCCTTTCACACGATCCAACGGGAGATCACCAATGGCTTTCGAAGTAACCCCGCTGCCCT
ATGCCGACACCGCGCTGGAA

Product: proton glutamate symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAESETRGGADDSGGLVTIRLPVWWTFGGLIAGLLTGLLLAGSNVIEPLLEVTGPVGTLWLRALQMTIIPLVAALLVLGI
TKMAQAARAGATARRMLLMVFGLLVFSGVASAISMPLLLDAFPPPSGASGILDAPSAETQQVPTIGDFIASLIAPNIIQA
AAETSTLPLTIFFALFAVAVARLPGAQRATMLRFFEALANTMLTIIGWVLWVAPVGVFALAVGVAARAGGEAFATLAHYI
VTVTAMGAIVLVAAYALGIIAGRISPAKFARSVLPAQAVAISTQSSLASLPAMLDSARRLGIREEVADFVLPLAVAIFRA
TSPAMNMAVAIYVAHLAGVELTPMLLAAGIAVAFVISIGSVSLPGSISFVISIGPIALAMGIPIEPLALLVAVEMLPDIM
RTLANVTMNVGLTAAVDREKSVSMQPESA

Sequences:

>Translated_429_residues
MAESETRGGADDSGGLVTIRLPVWWTFGGLIAGLLTGLLLAGSNVIEPLLEVTGPVGTLWLRALQMTIIPLVAALLVLGI
TKMAQAARAGATARRMLLMVFGLLVFSGVASAISMPLLLDAFPPPSGASGILDAPSAETQQVPTIGDFIASLIAPNIIQA
AAETSTLPLTIFFALFAVAVARLPGAQRATMLRFFEALANTMLTIIGWVLWVAPVGVFALAVGVAARAGGEAFATLAHYI
VTVTAMGAIVLVAAYALGIIAGRISPAKFARSVLPAQAVAISTQSSLASLPAMLDSARRLGIREEVADFVLPLAVAIFRA
TSPAMNMAVAIYVAHLAGVELTPMLLAAGIAVAFVISIGSVSLPGSISFVISIGPIALAMGIPIEPLALLVAVEMLPDIM
RTLANVTMNVGLTAAVDREKSVSMQPESA
>Mature_428_residues
AESETRGGADDSGGLVTIRLPVWWTFGGLIAGLLTGLLLAGSNVIEPLLEVTGPVGTLWLRALQMTIIPLVAALLVLGIT
KMAQAARAGATARRMLLMVFGLLVFSGVASAISMPLLLDAFPPPSGASGILDAPSAETQQVPTIGDFIASLIAPNIIQAA
AETSTLPLTIFFALFAVAVARLPGAQRATMLRFFEALANTMLTIIGWVLWVAPVGVFALAVGVAARAGGEAFATLAHYIV
TVTAMGAIVLVAAYALGIIAGRISPAKFARSVLPAQAVAISTQSSLASLPAMLDSARRLGIREEVADFVLPLAVAIFRAT
SPAMNMAVAIYVAHLAGVELTPMLLAAGIAVAFVISIGSVSLPGSISFVISIGPIALAMGIPIEPLALLVAVEMLPDIMR
TLANVTMNVGLTAAVDREKSVSMQPESA

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI223468566, Length=280, Percent_Identity=27.5, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223468564, Length=274, Percent_Identity=27.7372262773723, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI5032093, Length=280, Percent_Identity=27.5, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21314632, Length=404, Percent_Identity=25, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI40254478, Length=443, Percent_Identity=25.5079006772009, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI169790839, Length=251, Percent_Identity=27.8884462151394, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI4827012, Length=256, Percent_Identity=27.34375, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI66773030, Length=247, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790514, Length=428, Percent_Identity=25.9345794392523, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788024, Length=391, Percent_Identity=26.3427109974425, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789947, Length=371, Percent_Identity=25.3369272237197, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983099, Length=463, Percent_Identity=23.7580993520518, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71983106, Length=462, Percent_Identity=23.8095238095238, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17537407, Length=432, Percent_Identity=21.7592592592593, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996953, Length=250, Percent_Identity=27.2, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17541374, Length=249, Percent_Identity=29.718875502008, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI193206505, Length=416, Percent_Identity=23.7980769230769, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44049; Mature: 43918

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESETRGGADDSGGLVTIRLPVWWTFGGLIAGLLTGLLLAGSNVIEPLLEVTGPVGTLW
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
LRALQMTIIPLVAALLVLGITKMAQAARAGATARRMLLMVFGLLVFSGVASAISMPLLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AFPPPSGASGILDAPSAETQQVPTIGDFIASLIAPNIIQAAAETSTLPLTIFFALFAVAV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ARLPGAQRATMLRFFEALANTMLTIIGWVLWVAPVGVFALAVGVAARAGGEAFATLAHYI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VTVTAMGAIVLVAAYALGIIAGRISPAKFARSVLPAQAVAISTQSSLASLPAMLDSARRL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHC
GIREEVADFVLPLAVAIFRATSPAMNMAVAIYVAHLAGVELTPMLLAAGIAVAFVISIGS
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VSLPGSISFVISIGPIALAMGIPIEPLALLVAVEMLPDIMRTLANVTMNVGLTAAVDREK
CCCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
SVSMQPESA
CCCCCCCCC
>Mature Secondary Structure 
AESETRGGADDSGGLVTIRLPVWWTFGGLIAGLLTGLLLAGSNVIEPLLEVTGPVGTLW
CCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
LRALQMTIIPLVAALLVLGITKMAQAARAGATARRMLLMVFGLLVFSGVASAISMPLLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AFPPPSGASGILDAPSAETQQVPTIGDFIASLIAPNIIQAAAETSTLPLTIFFALFAVAV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ARLPGAQRATMLRFFEALANTMLTIIGWVLWVAPVGVFALAVGVAARAGGEAFATLAHYI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VTVTAMGAIVLVAAYALGIIAGRISPAKFARSVLPAQAVAISTQSSLASLPAMLDSARRL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHC
GIREEVADFVLPLAVAIFRATSPAMNMAVAIYVAHLAGVELTPMLLAAGIAVAFVISIGS
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VSLPGSISFVISIGPIALAMGIPIEPLALLVAVEMLPDIMRTLANVTMNVGLTAAVDREK
CCCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
SVSMQPESA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]