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The map label for this gene is rpoE [H]

Identifier: 85374873

GI number: 85374873

Start: 2077578

End: 2078192

Strand: Direct

Name: rpoE [H]

Synonym: ELI_10230

Alternate gene names: 85374873

Gene position: 2077578-2078192 (Clockwise)

Preceding gene: 85374872

Following gene: 85374876

Centisome position: 68.06

GC content: 65.2

Gene sequence:

>615_bases
ATGGCCGAAAAGAAGAAGCGGACCGCGGCCGAGAAGGTCGAGTTCAAGCGCGAGCTACAGGAAGTCATCCCGCACCTGCG
CGCTTTCGCGCGGGGGCTTTGCGGTCGCCCCGACATGGCGGACGACCTCGTTCAGGAGGCCCTGATGAAAGCCTGGGCCG
CGCAGGAACGGTTCGAGCCCGGGACCAGCATGCGCGCGTGGACCTTCGTGATCCTGCGCAATGCCTATCTCACCGACATG
CGGCGCAATCGTTTTCGCGGCGAATACGATGAAGGGGTTGCCGAGCGGATTCTGACCGCTCCCGCAGGCCAGGAGGAGCC
GATCCACCTGTCCGACATGCACCGCGCCCTGCTGACCCTCCCCCCGGAACGGCGCGAAGCGCTGCTGCTGGTGGGTGCCG
GCGGCTTTTCCTATGAGGAAGCGGCAGACATCTGCGGATGTGCCATCGGCACGATCAAGAGCCGGGTCGGCCGGGCGCGT
TCGGCCCTCAACGACATGCTCAAGGAAGGCACCATTCCGCAGCGCGCGCTTGGCGACGACATCGCGCATGATGCGATCAT
GGAAGAACTGGACGTTGTGGCAGCCGGTGACGGGGTATCCGAACCCACGCGTTAG

Upstream 100 bases:

>100_bases
GCGACCTTTACAAGTCGGTAGTGGACGAGCCGCTGCCCGACAGTTTCAAGGATTTGCTCGACAAGCTCGACGGCGACCCT
GAAGAGGGCGAGCAATAGTC

Downstream 100 bases:

>100_bases
TTGCCCCTCTCGCACGCAAGGAAAAGGCCCGCCCTCCAGTCTGGAGCGGCGGGCCTTTCTTCGTTCGCTATCCAGCGATC
AGGCGTAGGACCAGGTCGTA

Product: RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MAEKKKRTAAEKVEFKRELQEVIPHLRAFARGLCGRPDMADDLVQEALMKAWAAQERFEPGTSMRAWTFVILRNAYLTDM
RRNRFRGEYDEGVAERILTAPAGQEEPIHLSDMHRALLTLPPERREALLLVGAGGFSYEEAADICGCAIGTIKSRVGRAR
SALNDMLKEGTIPQRALGDDIAHDAIMEELDVVAAGDGVSEPTR

Sequences:

>Translated_204_residues
MAEKKKRTAAEKVEFKRELQEVIPHLRAFARGLCGRPDMADDLVQEALMKAWAAQERFEPGTSMRAWTFVILRNAYLTDM
RRNRFRGEYDEGVAERILTAPAGQEEPIHLSDMHRALLTLPPERREALLLVGAGGFSYEEAADICGCAIGTIKSRVGRAR
SALNDMLKEGTIPQRALGDDIAHDAIMEELDVVAAGDGVSEPTR
>Mature_203_residues
AEKKKRTAAEKVEFKRELQEVIPHLRAFARGLCGRPDMADDLVQEALMKAWAAQERFEPGTSMRAWTFVILRNAYLTDMR
RNRFRGEYDEGVAERILTAPAGQEEPIHLSDMHRALLTLPPERREALLLVGAGGFSYEEAADICGCAIGTIKSRVGRARS
ALNDMLKEGTIPQRALGDDIAHDAIMEELDVVAAGDGVSEPTR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=139, Percent_Identity=32.3741007194245, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22649; Mature: 22518

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKKKRTAAEKVEFKRELQEVIPHLRAFARGLCGRPDMADDLVQEALMKAWAAQERFEP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GTSMRAWTFVILRNAYLTDMRRNRFRGEYDEGVAERILTAPAGQEEPIHLSDMHRALLTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
PPERREALLLVGAGGFSYEEAADICGCAIGTIKSRVGRARSALNDMLKEGTIPQRALGDD
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IAHDAIMEELDVVAAGDGVSEPTR
HHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AEKKKRTAAEKVEFKRELQEVIPHLRAFARGLCGRPDMADDLVQEALMKAWAAQERFEP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GTSMRAWTFVILRNAYLTDMRRNRFRGEYDEGVAERILTAPAGQEEPIHLSDMHRALLTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
PPERREALLLVGAGGFSYEEAADICGCAIGTIKSRVGRARSALNDMLKEGTIPQRALGDD
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IAHDAIMEELDVVAAGDGVSEPTR
HHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]