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The map label for this gene is phbC [H]

Identifier: 85374862

GI number: 85374862

Start: 2065656

End: 2067533

Strand: Direct

Name: phbC [H]

Synonym: ELI_10175

Alternate gene names: 85374862

Gene position: 2065656-2067533 (Clockwise)

Preceding gene: 85374855

Following gene: 85374863

Centisome position: 67.67

GC content: 60.65

Gene sequence:

>1878_bases
ATGAGCGACACCAAAGACCCTTTTTCCGATCCCACGCAGGCATTTTCGAAGCTGTTCGACTTGCAGGACGAAGCGGCGCA
GTCGATCTTCGGCCAGATGATGCCGGGCGTGGCGCCGAAAATGCCCGATCTGTCCGAGGCAGGCGCGTGGGCCAAGAATG
CTGCGACCATGCAAAGCATGTGGCTCAGCTTCCAGGCGGAGCAGGCGGCCAAGATCTCCAAGGAAATGCCCGACCCGACG
CGCTGGATGGCCTATCTCGACAGCTTTCACAAACGCATGCCGATGGTCGATATGGCCGCGCAGCAGCAGTTCTGGGGCGA
TTCGATGAGCATGTGGAAGGGCGTGCTCGGCCAGTTCGGCGGGCAGTTCGGCGACAATTCGGATGCCGCGCCCGAACTCC
CGCGCAAGGACAAGCGCTTTCGCGACGAGCGCTGGCAGAACAATCCGGTCTTCGCGCTGATCCACCAGACCTACCTGCTG
ATGGCAGAGCAACTGGAGAGCATGGTCGACAGGCTGGACGGCGTGGAACCCGACAAGAAGGAACAATTGCGTTTCTCCGT
GAAAGCCGTCGTCGATGCTCTCAGCCCGTCGAATTTCGCGGCGACCAACCCGGTTGTGCTCGAGCGAACGCTGGAGACCA
AGGGCGAGAATCTCGTCAAGGGCATGCAGCACATGCTCGACGACATGCGGCGCGGCCAGCTGACCCATACCGATCCCGAC
GCGTTCACGCTGGGCGAGAACATCGCGGTCACGCCGGGCAAGGTCGTGCACGAAACGCCGATGTATCAGCTGATCCAGTA
TTCGCCCTCGACCGAGGAAGTGCTGGAAGTGCCACTGGTCATCTTCCCGCCGTGGATCAACCGTTTTTACATTCTCGACC
TCGATCCGGAAAAGAGCTTCATCCGATGGGCGGTCGAGCAGGGCCTGACGGTCTTCGTGGTCAGCTGGAAATCGGCCGAC
GCCTCCATGAAAGACGTAGTGTGGGACGATTACATCCGCAGTCAGCTCGAAACGATCGATCACATCCGCGGGCGGCTGAA
GGTGCCGCAGGTCCACGCGATCGGTTATTGCGTGGCCGGAACTACCCTTGCGGCTACGCTGGCGATCCTCGCTCGGCGCG
GGGAGCAGGACAAGGTGAAGAGCGCGACCTTCTTCACCGCGCAGGTCGATTTCGAGAAGGCTGGTGAGCTGCTCCACTTC
ATCGACGACAACCAGGTCGCCGCGATCGAAGCGCTATCGGGCGAAGGCTATCTCGACGGGCGCTACATGGCCGCGACATT
CAATCTGTTGCGCGGGACCGACCTGATCTGGAACTATGTCGTCAACAATTACCTGCTGGGCGAGGACTATCCGGCCTTCG
ACCTGCTACACTGGAACGGGGACGTCACCAACCTGCCTGCGAAATGGCACAAGGACTATATTCAAGAGCTGTATCGCGAC
AATCGGCTGGTCAAGCCGGACTCGCTGAGCGCTGATGGCACGCCGATCGATCTCTCGCTCGTGTCCGTCCCGACCTATGT
GCAGGCCGGCAAGGAAGACCACATCGCGCCGGCCGAAAGTGTCTGGCGATTGCGCGAACATTTCACCGGTCCGATCCGCT
TTGTGTTGGCGGGTTCGGGCCATATCGCTGGCGTCGTCAACCCGCCCGACAGCGGCAAATACCAGTACTGGATCAACGAA
GATCCGGACGTGACGACGCTTGAAGCTTTCCGCGAAGGCGCAACCGAGCATCCGGGAAGCTGGTGGCCCGACTGGATCAA
ATGGGTGCGCAGCCAGGACAGCAAGACCGTAAAGGCAAGCGGCAAACGCAAGCCCGGATCGATCAAGTCCGACAAGGTGA
TCGAAGACGCGCCAGGTCGCTATGTGGCCGCAAGATAG

Upstream 100 bases:

>100_bases
AAAGCTCTAGCACGCGCAATCGAATGCATAATGCGCGCATCGAACACCGCCCTTGTCAGACCAGTCGCCAGCGCCCAAGA
AACAGGGCAGGAGACCCGGC

Downstream 100 bases:

>100_bases
TTGCGCTCACTAGATTTTCTCGCACAGTAACAAGGCGTTAGAGCGCATTATCCCATTTATTGTGCACTGCACAAAAATCC
CTTGACTTCGCAGTTGCAAT

Product: poly-3-hydroxybutyrate synthase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSDTKDPFSDPTQAFSKLFDLQDEAAQSIFGQMMPGVAPKMPDLSEAGAWAKNAATMQSMWLSFQAEQAAKISKEMPDPT
RWMAYLDSFHKRMPMVDMAAQQQFWGDSMSMWKGVLGQFGGQFGDNSDAAPELPRKDKRFRDERWQNNPVFALIHQTYLL
MAEQLESMVDRLDGVEPDKKEQLRFSVKAVVDALSPSNFAATNPVVLERTLETKGENLVKGMQHMLDDMRRGQLTHTDPD
AFTLGENIAVTPGKVVHETPMYQLIQYSPSTEEVLEVPLVIFPPWINRFYILDLDPEKSFIRWAVEQGLTVFVVSWKSAD
ASMKDVVWDDYIRSQLETIDHIRGRLKVPQVHAIGYCVAGTTLAATLAILARRGEQDKVKSATFFTAQVDFEKAGELLHF
IDDNQVAAIEALSGEGYLDGRYMAATFNLLRGTDLIWNYVVNNYLLGEDYPAFDLLHWNGDVTNLPAKWHKDYIQELYRD
NRLVKPDSLSADGTPIDLSLVSVPTYVQAGKEDHIAPAESVWRLREHFTGPIRFVLAGSGHIAGVVNPPDSGKYQYWINE
DPDVTTLEAFREGATEHPGSWWPDWIKWVRSQDSKTVKASGKRKPGSIKSDKVIEDAPGRYVAAR

Sequences:

>Translated_625_residues
MSDTKDPFSDPTQAFSKLFDLQDEAAQSIFGQMMPGVAPKMPDLSEAGAWAKNAATMQSMWLSFQAEQAAKISKEMPDPT
RWMAYLDSFHKRMPMVDMAAQQQFWGDSMSMWKGVLGQFGGQFGDNSDAAPELPRKDKRFRDERWQNNPVFALIHQTYLL
MAEQLESMVDRLDGVEPDKKEQLRFSVKAVVDALSPSNFAATNPVVLERTLETKGENLVKGMQHMLDDMRRGQLTHTDPD
AFTLGENIAVTPGKVVHETPMYQLIQYSPSTEEVLEVPLVIFPPWINRFYILDLDPEKSFIRWAVEQGLTVFVVSWKSAD
ASMKDVVWDDYIRSQLETIDHIRGRLKVPQVHAIGYCVAGTTLAATLAILARRGEQDKVKSATFFTAQVDFEKAGELLHF
IDDNQVAAIEALSGEGYLDGRYMAATFNLLRGTDLIWNYVVNNYLLGEDYPAFDLLHWNGDVTNLPAKWHKDYIQELYRD
NRLVKPDSLSADGTPIDLSLVSVPTYVQAGKEDHIAPAESVWRLREHFTGPIRFVLAGSGHIAGVVNPPDSGKYQYWINE
DPDVTTLEAFREGATEHPGSWWPDWIKWVRSQDSKTVKASGKRKPGSIKSDKVIEDAPGRYVAAR
>Mature_624_residues
SDTKDPFSDPTQAFSKLFDLQDEAAQSIFGQMMPGVAPKMPDLSEAGAWAKNAATMQSMWLSFQAEQAAKISKEMPDPTR
WMAYLDSFHKRMPMVDMAAQQQFWGDSMSMWKGVLGQFGGQFGDNSDAAPELPRKDKRFRDERWQNNPVFALIHQTYLLM
AEQLESMVDRLDGVEPDKKEQLRFSVKAVVDALSPSNFAATNPVVLERTLETKGENLVKGMQHMLDDMRRGQLTHTDPDA
FTLGENIAVTPGKVVHETPMYQLIQYSPSTEEVLEVPLVIFPPWINRFYILDLDPEKSFIRWAVEQGLTVFVVSWKSADA
SMKDVVWDDYIRSQLETIDHIRGRLKVPQVHAIGYCVAGTTLAATLAILARRGEQDKVKSATFFTAQVDFEKAGELLHFI
DDNQVAAIEALSGEGYLDGRYMAATFNLLRGTDLIWNYVVNNYLLGEDYPAFDLLHWNGDVTNLPAKWHKDYIQELYRDN
RLVKPDSLSADGTPIDLSLVSVPTYVQAGKEDHIAPAESVWRLREHFTGPIRFVLAGSGHIAGVVNPPDSGKYQYWINED
PDVTTLEAFREGATEHPGSWWPDWIKWVRSQDSKTVKASGKRKPGSIKSDKVIEDAPGRYVAAR

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 70462; Mature: 70331

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure 
SDTKDPFSDPTQAFSKLFDLQDEAAQSIFGQMMPGVAPKMPDLSEAGAWAKNAATMQSM
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHH
WLSFQAEQAAKISKEMPDPTRWMAYLDSFHKRMPMVDMAAQQQFWGDSMSMWKGVLGQFG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHC
GQFGDNSDAAPELPRKDKRFRDERWQNNPVFALIHQTYLLMAEQLESMVDRLDGVEPDKK
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHH
EQLRFSVKAVVDALSPSNFAATNPVVLERTLETKGENLVKGMQHMLDDMRRGQLTHTDPD
HHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
AFTLGENIAVTPGKVVHETPMYQLIQYSPSTEEVLEVPLVIFPPWINRFYILDLDPEKSF
CEECCCCEEECCCHHHHCCHHHHHHCCCCCHHHHHHCCEEEECCCCCEEEEEEECCCHHH
IRWAVEQGLTVFVVSWKSADASMKDVVWDDYIRSQLETIDHIRGRLKVPQVHAIGYCVAG
HHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
TTLAATLAILARRGEQDKVKSATFFTAQVDFEKAGELLHFIDDNQVAAIEALSGEGYLDG
HHHHHHHHHHHHCCCCHHHCCEEEEEEEECHHHHCCEEEHCCCCCEEEEEEECCCCCCCC
RYMAATFNLLRGTDLIWNYVVNNYLLGEDYPAFDLLHWNGDVTNLPAKWHKDYIQELYRD
EEHHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
NRLVKPDSLSADGTPIDLSLVSVPTYVQAGKEDHIAPAESVWRLREHFTGPIRFVLAGSG
CCCCCCCCCCCCCCCEEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
HIAGVVNPPDSGKYQYWINEDPDVTTLEAFREGATEHPGSWWPDWIKWVRSQDSKTVKAS
CEEEEECCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECC
GKRKPGSIKSDKVIEDAPGRYVAAR
CCCCCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]