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The map label for this gene is thiC

Identifier: 85374787

GI number: 85374787

Start: 1986798

End: 1988687

Strand: Reverse

Name: thiC

Synonym: ELI_09800

Alternate gene names: 85374787

Gene position: 1988687-1986798 (Counterclockwise)

Preceding gene: 85374788

Following gene: 85374786

Centisome position: 65.15

GC content: 65.03

Gene sequence:

>1890_bases
ATGGCAGACATCAATTCCCCGCTCGAGATCGGCGTCACCACGGGGCCCATTCGCGGTTCAAAGAAAATCTATGTCGGCGC
GCGCACGGGTTCCGGCGTGCGCGTCGCCATGCGCGAGATCCAGCTGGAGGGCGGCGAGGAGCCGGTGCGGGTCTACGACA
CCTCGGGCCCCTATACCGACCCCGAGGTGAGCATCGACATCCAGGCCGGCCTGCCCGCGCTGCGGCGCGAGTGGATCATG
GGCCGCGGCGACGTCGAGGAATATGCCGCGCGCGCAGTGAAGCCGGAGGATAACGGCCAACTCGGCCCTGACCGTTCGGG
CGGTGTCCCGGCCTTCCCCAACGTGGCCAGGACCGTGCTGCGCGCGAAGCCGGGCCAGAATGTCAGCCAGATGCATTACG
CCCGCAAAGGCATCATCACGCCCGAGATGGAATATGTCGCTGAGCGTGAGAATGTCGGCCGGGCGAAGCTCGAAACCGCG
CCCGACGGGCAGGCATGGGGCGCCGAAATCCCGCAGGAGATCACGCCCGAATTCGTGCGCGACGAGGTCGCCCGCGGCCG
CGCGATCATCCCCAACAACATCAACCATCCCGAAACCGAACCGATGGCGATCGGGCGCAATTTCCTCGTCAAGATCAACG
CCAATATCGGCAACTCCGCCGTGGCATCGGATGTGGCGGCGGAAGTCGACAAGATGGTCTGGTCGATCCGCTGGGGCGCG
GACACCGTCATGGACCTCTCCACGGGGCGCAACATCCACGACACGCGCGAATGGATCATCCGCAACTCGCCCGTCCCCAT
CGGCACCGTACCGATCTACCAGGCGCTGGAGAAGGTCGGCGGCATCGCCGAGGACCTGACCTGGGACATCTTCCGCGACA
CGCTGATCGAACAGGCCGAACAGGGCGTCGACTATTTCACCATCCATGCCGGCGTGCGCCTGCCCTACGTCCCGATGACC
GCCAAGCGCGTCACCGGCATCGTGTCGCGCGGCGGCTCCATCATGGCGAAATGGTGCCTCGCGCATCACAAGGAGAGTTT
CCTCTACGAGCGCTTCGACGAGATCACCGAGATCATGAAGGCCTATGACATCGCCTATTCGCTGGGCGACGGCCTGCGCC
CCGGCTCCATCGCCGACGCCAACGACGAAGCCCAGTTCGCCGAGCTCTACACGCTGGGCGAGCTCACCAAGCGCGCCTGG
GAGCAGGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGATGCACAAGATCAAGGAGAACATGGACAAGCAGCT
GGAGGCGTGCGGCGAAGCGCCGTTCTACACGCTCGGCCCGCTCGTGACCGACATCGCGCCGGGATACGACCACATCACGA
GCGGCATCGGCGCGGCGCAGATCGGCTGGTATGGCACCGCGATGCTCTGCTACGTCACGCCCAAGGAACACCTCGGCCTG
CCCGACCGCGACGATGTGAAGGTGGGCGTGGTGACCTACAAGCTCGCCGCCCACGCCGCCGACCTCGCCAAGGGGCATCC
TGCGGCCAAGGTCCGCGACGACGCGCTGAGCAAGGCCCGCTTCGAATTCCGCTGGCGGGATCAGTTCAATCTCAGCCTCG
ACCCCGACACGGCCGAACAATACCACGACCAGACCCTCCCCGCAGAAGGCGCCAAGACCGCCCACTTCTGCTCGATGTGC
GGCCCGAAATTCTGCTCGATGAAGATCACCCAGGAAGTGCGGGATTTTGCCGCCAAGCAGAACTCGGACAGCTATCTCGC
GAGCGAGAACATCAAGCGCGAAACCTCAGCCGAAGAGGCCGAGGAAGCGCGCGAGGGAATGTCCGACATGGCCGAACTTT
ACAAGGCCAAGGGCGAGAGGCTCTACCTACCCGAAAGCGAGGCGGACTGA

Upstream 100 bases:

>100_bases
TGCGCGTGTGGGCGGGCCTTTCCGCGCTGCTCGGGGCCGCCTTCATCTTCGTCGCGCTTTCGCGGCTGCAAACCATCTGA
TCGACTGAAAGAGACTGACC

Downstream 100 bases:

>100_bases
CCCTCGCGAGGGCATGGGGGGAGTCGGAGCGCTCCCGCGGAGCGCGGAGACGTGCAAAGCACAAATGAGCGAAGTCTACC
GCGAGAAGGGCGAGAAGTTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MADINSPLEIGVTTGPIRGSKKIYVGARTGSGVRVAMREIQLEGGEEPVRVYDTSGPYTDPEVSIDIQAGLPALRREWIM
GRGDVEEYAARAVKPEDNGQLGPDRSGGVPAFPNVARTVLRAKPGQNVSQMHYARKGIITPEMEYVAERENVGRAKLETA
PDGQAWGAEIPQEITPEFVRDEVARGRAIIPNNINHPETEPMAIGRNFLVKINANIGNSAVASDVAAEVDKMVWSIRWGA
DTVMDLSTGRNIHDTREWIIRNSPVPIGTVPIYQALEKVGGIAEDLTWDIFRDTLIEQAEQGVDYFTIHAGVRLPYVPMT
AKRVTGIVSRGGSIMAKWCLAHHKESFLYERFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYTLGELTKRAW
EQDVQVMIEGPGHVPMHKIKENMDKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGL
PDRDDVKVGVVTYKLAAHAADLAKGHPAAKVRDDALSKARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGAKTAHFCSMC
GPKFCSMKITQEVRDFAAKQNSDSYLASENIKRETSAEEAEEAREGMSDMAELYKAKGERLYLPESEAD

Sequences:

>Translated_629_residues
MADINSPLEIGVTTGPIRGSKKIYVGARTGSGVRVAMREIQLEGGEEPVRVYDTSGPYTDPEVSIDIQAGLPALRREWIM
GRGDVEEYAARAVKPEDNGQLGPDRSGGVPAFPNVARTVLRAKPGQNVSQMHYARKGIITPEMEYVAERENVGRAKLETA
PDGQAWGAEIPQEITPEFVRDEVARGRAIIPNNINHPETEPMAIGRNFLVKINANIGNSAVASDVAAEVDKMVWSIRWGA
DTVMDLSTGRNIHDTREWIIRNSPVPIGTVPIYQALEKVGGIAEDLTWDIFRDTLIEQAEQGVDYFTIHAGVRLPYVPMT
AKRVTGIVSRGGSIMAKWCLAHHKESFLYERFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYTLGELTKRAW
EQDVQVMIEGPGHVPMHKIKENMDKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGL
PDRDDVKVGVVTYKLAAHAADLAKGHPAAKVRDDALSKARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGAKTAHFCSMC
GPKFCSMKITQEVRDFAAKQNSDSYLASENIKRETSAEEAEEAREGMSDMAELYKAKGERLYLPESEAD
>Mature_628_residues
ADINSPLEIGVTTGPIRGSKKIYVGARTGSGVRVAMREIQLEGGEEPVRVYDTSGPYTDPEVSIDIQAGLPALRREWIMG
RGDVEEYAARAVKPEDNGQLGPDRSGGVPAFPNVARTVLRAKPGQNVSQMHYARKGIITPEMEYVAERENVGRAKLETAP
DGQAWGAEIPQEITPEFVRDEVARGRAIIPNNINHPETEPMAIGRNFLVKINANIGNSAVASDVAAEVDKMVWSIRWGAD
TVMDLSTGRNIHDTREWIIRNSPVPIGTVPIYQALEKVGGIAEDLTWDIFRDTLIEQAEQGVDYFTIHAGVRLPYVPMTA
KRVTGIVSRGGSIMAKWCLAHHKESFLYERFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYTLGELTKRAWE
QDVQVMIEGPGHVPMHKIKENMDKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGLP
DRDDVKVGVVTYKLAAHAADLAKGHPAAKVRDDALSKARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGAKTAHFCSMCG
PKFCSMKITQEVRDFAAKQNSDSYLASENIKRETSAEEAEEAREGMSDMAELYKAKGERLYLPESEAD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=632, Percent_Identity=65.8227848101266, Blast_Score=840, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_ERYLH (Q2N8D9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458849.1
- STRING:   Q2N8D9
- GeneID:   3871569
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_09800
- NMPDR:   fig|314225.3.peg.1078
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   HLRFEAG
- PhylomeDB:   Q2N8D9
- ProtClustDB:   PRK09284
- BioCyc:   ELIT314225:ELI_09800-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 69372; Mature: 69241

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: BINDING 215-215 BINDING 244-244 BINDING 273-273 BINDING 309-309 BINDING 409-409 BINDING 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADINSPLEIGVTTGPIRGSKKIYVGARTGSGVRVAMREIQLEGGEEPVRVYDTSGPYTD
CCCCCCCCEECEEECCCCCCCEEEEEECCCCCHHEEEHHHHHCCCCCCEEEEECCCCCCC
PEVSIDIQAGLPALRREWIMGRGDVEEYAARAVKPEDNGQLGPDRSGGVPAFPNVARTVL
CCEEEEECCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RAKPGQNVSQMHYARKGIITPEMEYVAERENVGRAKLETAPDGQAWGAEIPQEITPEFVR
HCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHCCHHHHH
DEVARGRAIIPNNINHPETEPMAIGRNFLVKINANIGNSAVASDVAAEVDKMVWSIRWGA
HHHHCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCC
DTVMDLSTGRNIHDTREWIIRNSPVPIGTVPIYQALEKVGGIAEDLTWDIFRDTLIEQAE
CCEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QGVDYFTIHAGVRLPYVPMTAKRVTGIVSRGGSIMAKWCLAHHKESFLYERFDEITEIMK
CCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYDIAYSLGDGLRPGSIADANDEAQFAELYTLGELTKRAWEQDVQVMIEGPGHVPMHKIK
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
ENMDKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGL
HHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHCCCCEEEEEEECCHHHCCC
PDRDDVKVGVVTYKLAAHAADLAKGHPAAKVRDDALSKARFEFRWRDQFNLSLDPDTAEQ
CCCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCEEECCCHHHH
YHDQTLPAEGAKTAHFCSMCGPKFCSMKITQEVRDFAAKQNSDSYLASENIKRETSAEEA
HHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHCCHHHH
EEAREGMSDMAELYKAKGERLYLPESEAD
HHHHHHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure 
ADINSPLEIGVTTGPIRGSKKIYVGARTGSGVRVAMREIQLEGGEEPVRVYDTSGPYTD
CCCCCCCEECEEECCCCCCCEEEEEECCCCCHHEEEHHHHHCCCCCCEEEEECCCCCCC
PEVSIDIQAGLPALRREWIMGRGDVEEYAARAVKPEDNGQLGPDRSGGVPAFPNVARTVL
CCEEEEECCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RAKPGQNVSQMHYARKGIITPEMEYVAERENVGRAKLETAPDGQAWGAEIPQEITPEFVR
HCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHCCHHHHH
DEVARGRAIIPNNINHPETEPMAIGRNFLVKINANIGNSAVASDVAAEVDKMVWSIRWGA
HHHHCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCC
DTVMDLSTGRNIHDTREWIIRNSPVPIGTVPIYQALEKVGGIAEDLTWDIFRDTLIEQAE
CCEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QGVDYFTIHAGVRLPYVPMTAKRVTGIVSRGGSIMAKWCLAHHKESFLYERFDEITEIMK
CCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYDIAYSLGDGLRPGSIADANDEAQFAELYTLGELTKRAWEQDVQVMIEGPGHVPMHKIK
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
ENMDKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGL
HHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHCCCCEEEEEEECCHHHCCC
PDRDDVKVGVVTYKLAAHAADLAKGHPAAKVRDDALSKARFEFRWRDQFNLSLDPDTAEQ
CCCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCEEECCCHHHH
YHDQTLPAEGAKTAHFCSMCGPKFCSMKITQEVRDFAAKQNSDSYLASENIKRETSAEEA
HHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHCCHHHH
EEAREGMSDMAELYKAKGERLYLPESEAD
HHHHHHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA