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The map label for this gene is hisB

Identifier: 85374741

GI number: 85374741

Start: 1950839

End: 1951432

Strand: Reverse

Name: hisB

Synonym: ELI_09570

Alternate gene names: 85374741

Gene position: 1951432-1950839 (Counterclockwise)

Preceding gene: 85374746

Following gene: 85374740

Centisome position: 63.93

GC content: 61.45

Gene sequence:

>594_bases
ATGCGCACCGGCAGAATTGAGCGAAATACCGCGGAAACGAGGATCCTGATCGAAGTGAACCTCGACGGGAGAGGGAACTA
TGACGTTTCCACCGGGATCGGCTTTCTCGATCACATGGTCGAACAGTTCTCCAAGCATTCGCTGATCGATGTGACGATGA
AAGTCGACGGCGACCTGCATGTCGACCAGCACCACACCACCGAAGATAGCGCGATCGCGCTGGGGCAGGCGCTCGGCGAA
GCGCTGGGCGACAAGGCCGGGATTGGGCGCTACGGCCAGGCCTATTCGCCGATGGACGAAACGCTGAGCCGCGTCGCTTT
GGACATTTCTGGCCGTCCCTACACTGTCTTTCGCGCGGGCTTCAGCCAGGAAAAGCTCGGCGAATGGGACACCGAGCTGA
TCGAGCACTGGTTCCAGTCGGTCGCGCAGGCGGCGGGAATCACGCTGCATATCGAATTGCTCTACGGCACCAACAACCAC
CACATCTGCGAGAGCATCTACAAGGGCTTCGCCCGCGCGATGCGGCAGGCGGTGGAACTGGATGGGCGCAAGGGCGGGGC
GATCCCGAGCACCAAGGGGCAGTTGGGTGGCTGA

Upstream 100 bases:

>100_bases
GGGGGTATCCTCGGTCATGTGCTTCAACTGACGATACCCGCGGCCCCGGTCAAGCGCAGAATGCTTGTATATCCGCCGCT
GAAAGCTATAGCCCGCGCCC

Downstream 100 bases:

>100_bases
GAGGATCGCGCTGATCGACTATGGCGCGGGCAACCTGCATTCAGTTCACAACGCATTGAAGGCTGCCGGAGCGCACGGGG
TCAAAGTTACGCATAATCCC

Product: imidazoleglycerol-phosphate dehydratase

Products: NA

Alternate protein names: IGPD

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MRTGRIERNTAETRILIEVNLDGRGNYDVSTGIGFLDHMVEQFSKHSLIDVTMKVDGDLHVDQHHTTEDSAIALGQALGE
ALGDKAGIGRYGQAYSPMDETLSRVALDISGRPYTVFRAGFSQEKLGEWDTELIEHWFQSVAQAAGITLHIELLYGTNNH
HICESIYKGFARAMRQAVELDGRKGGAIPSTKGQLGG

Sequences:

>Translated_197_residues
MRTGRIERNTAETRILIEVNLDGRGNYDVSTGIGFLDHMVEQFSKHSLIDVTMKVDGDLHVDQHHTTEDSAIALGQALGE
ALGDKAGIGRYGQAYSPMDETLSRVALDISGRPYTVFRAGFSQEKLGEWDTELIEHWFQSVAQAAGITLHIELLYGTNNH
HICESIYKGFARAMRQAVELDGRKGGAIPSTKGQLGG
>Mature_197_residues
MRTGRIERNTAETRILIEVNLDGRGNYDVSTGIGFLDHMVEQFSKHSLIDVTMKVDGDLHVDQHHTTEDSAIALGQALGE
ALGDKAGIGRYGQAYSPMDETLSRVALDISGRPYTVFRAGFSQEKLGEWDTELIEHWFQSVAQAAGITLHIELLYGTNNH
HICESIYKGFARAMRQAVELDGRKGGAIPSTKGQLGG

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=194, Percent_Identity=46.3917525773196, Blast_Score=184, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6324776, Length=168, Percent_Identity=48.8095238095238, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_ERYLH (Q2N8I5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458803.1
- ProteinModelPortal:   Q2N8I5
- SMR:   Q2N8I5
- STRING:   Q2N8I5
- GeneID:   3869497
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_09570
- NMPDR:   fig|314225.3.peg.1125
- eggNOG:   COG0131
- HOGENOM:   HBG289010
- OMA:   TLHVETL
- PhylomeDB:   Q2N8I5
- ProtClustDB:   PRK00951
- BioCyc:   ELIT314225:ELI_09570-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00076
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR020568

Pfam domain/function: PF00475 IGPD; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =4.2.1.19

Molecular weight: Translated: 21615; Mature: 21615

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTGRIERNTAETRILIEVNLDGRGNYDVSTGIGFLDHMVEQFSKHSLIDVTMKVDGDLH
CCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEE
VDQHHTTEDSAIALGQALGEALGDKAGIGRYGQAYSPMDETLSRVALDISGRPYTVFRAG
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCEEEEECC
FSQEKLGEWDTELIEHWFQSVAQAAGITLHIELLYGTNNHHICESIYKGFARAMRQAVEL
CCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
DGRKGGAIPSTKGQLGG
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTGRIERNTAETRILIEVNLDGRGNYDVSTGIGFLDHMVEQFSKHSLIDVTMKVDGDLH
CCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEE
VDQHHTTEDSAIALGQALGEALGDKAGIGRYGQAYSPMDETLSRVALDISGRPYTVFRAG
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCEEEEECC
FSQEKLGEWDTELIEHWFQSVAQAAGITLHIELLYGTNNHHICESIYKGFARAMRQAVEL
CCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
DGRKGGAIPSTKGQLGG
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA