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The map label for this gene is tolB

Identifier: 85374728

GI number: 85374728

Start: 1943056

End: 1944471

Strand: Reverse

Name: tolB

Synonym: ELI_09505

Alternate gene names: 85374728

Gene position: 1944471-1943056 (Counterclockwise)

Preceding gene: 85374729

Following gene: 85374727

Centisome position: 63.7

GC content: 65.18

Gene sequence:

>1416_bases
TTGTCGAGAGGCACGCTGCTCGGTGCCTTCGCCCTGGTGGGCCTGATTACTCCGCTAGCCGCGCAGGACCTCGCCTCGCC
GCCGCCACGCGGTGGTGCGGTCGAGCAGGTCGCCGCCGATGCCTCGTCGGCGCAGGATGGTGATGAAGGCGGTCTCACCG
GCTCGGTCTCCTTCGAGGGTAATCTCGACGATCTCGGCATTGCCATCCCGGCCTTCGCCACCGACCGGGACGTTGCAACT
CCTGCCAACGCGCAGGGTACGGGCGCGCTCGGGGCCGAACTTGCCCGGGTAATCAGCAACAATCTGCGCAACAACGGACT
GTTCAAGCCTTCGGGACCCGACGGGCTGCCGCGGCCGACCTTTCCGCAGATCACCGATCCGCAATGGGCGGTCTGGTCCA
ACCGCGGCGCGGAAATGCTCGTTCACGGCTACGTCCGCGCGCGCGGTGACGGCAATCTTGTCGTCGGCTGCTATCTTTAC
GACATGGCCCTCCGCGACGAGCTCGTCCGCTCCGGCTGGGTGGTGCCGCCCGCCGATTGGCGCCGCGCGGCGCACAAGTG
TTCGGACCTCGTCTATTCGCGCCTGACGGGCGAAAGTCCGTTCTTCGATAGCCGTATCGCCTACATCGCCGAAACCGGTC
CCAAGGATAATCGCACCAAGCGGCTGGCCATCATGGACAGCGACGGGGCCAACCATCGCTTCATCACCACCGGTCGCGCG
ACGGCGCTGACACCGCGCTACTCGCCCGATTACCGCCGCCTGCTCTATCTCAGCTACGTCGACGGCAATCCGCGCATCTA
TATCTACGATATCGGCACAGGGCGGCAGCAATTGGTGACCGAGAGCACCAACCCGACCTTCGCCCCGCGCTGGAGCCCCG
ACGGACGCTGGATCCTCTATTCCATGGCGGTGAACGGCAATACCGACATCTATCGCGTCAGCGCCGAAGGCGGGCCGAGC
GTGCGGTTGACCACCGAGCCGGGCATCGACGTCGGCGGCTCGTATTCGCCCGATGGCAGTCAGATCGTGTTCGAAAGCGA
CCGCTCGGGCAGCCAGCAGGTCTACATCATGAATTCGGACGGTACGAACCAGCGCCGCATCAGCTTCTTCGGCGGGCGCG
CGGCTACGCCGGAATGGAGCCCGCGCGGCGACCAGATCGCCTTCACCTACATCCCGGGCGATTTCAACATAGCGGTCATG
AGCCCCAACGGGCGCAACTTCCGCAAACTGTCGAACGGTTGGCAAGACGAAGCGCCGACCTGGGCACCCAACGGCCGGAT
CATCCAGTTCTTCCGTACCGAGCGTAATACCGGGCGCACGGCGCTCTACCAGGTCGACCTGACCGGGGACAATCTGCGGC
GGCTCAACACGCCTGTCGACGCTTCGGATCCGGCCTGGGGACCGATTTTGCCATAA

Upstream 100 bases:

>100_bases
GCTCTCGTCTTCGCTCCGCTTCGCTCGATCGCTGCTCGGGACGAACGGAATTTTTTTGAACCGTTGGTCTCGTGCAGGCA
TCGAGCAAGGTCGAGATGCC

Downstream 100 bases:

>100_bases
TCGCGTTCTATCCAGGACACTTGAAGAACAGGGGCGGGTCGTGCCGCACAAACAGGGCAATCTATATTGGTGAGGAACTA
GCCATGAATACCAAATTCGC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSRGTLLGAFALVGLITPLAAQDLASPPPRGGAVEQVAADASSAQDGDEGGLTGSVSFEGNLDDLGIAIPAFATDRDVAT
PANAQGTGALGAELARVISNNLRNNGLFKPSGPDGLPRPTFPQITDPQWAVWSNRGAEMLVHGYVRARGDGNLVVGCYLY
DMALRDELVRSGWVVPPADWRRAAHKCSDLVYSRLTGESPFFDSRIAYIAETGPKDNRTKRLAIMDSDGANHRFITTGRA
TALTPRYSPDYRRLLYLSYVDGNPRIYIYDIGTGRQQLVTESTNPTFAPRWSPDGRWILYSMAVNGNTDIYRVSAEGGPS
VRLTTEPGIDVGGSYSPDGSQIVFESDRSGSQQVYIMNSDGTNQRRISFFGGRAATPEWSPRGDQIAFTYIPGDFNIAVM
SPNGRNFRKLSNGWQDEAPTWAPNGRIIQFFRTERNTGRTALYQVDLTGDNLRRLNTPVDASDPAWGPILP

Sequences:

>Translated_471_residues
MSRGTLLGAFALVGLITPLAAQDLASPPPRGGAVEQVAADASSAQDGDEGGLTGSVSFEGNLDDLGIAIPAFATDRDVAT
PANAQGTGALGAELARVISNNLRNNGLFKPSGPDGLPRPTFPQITDPQWAVWSNRGAEMLVHGYVRARGDGNLVVGCYLY
DMALRDELVRSGWVVPPADWRRAAHKCSDLVYSRLTGESPFFDSRIAYIAETGPKDNRTKRLAIMDSDGANHRFITTGRA
TALTPRYSPDYRRLLYLSYVDGNPRIYIYDIGTGRQQLVTESTNPTFAPRWSPDGRWILYSMAVNGNTDIYRVSAEGGPS
VRLTTEPGIDVGGSYSPDGSQIVFESDRSGSQQVYIMNSDGTNQRRISFFGGRAATPEWSPRGDQIAFTYIPGDFNIAVM
SPNGRNFRKLSNGWQDEAPTWAPNGRIIQFFRTERNTGRTALYQVDLTGDNLRRLNTPVDASDPAWGPILP
>Mature_470_residues
SRGTLLGAFALVGLITPLAAQDLASPPPRGGAVEQVAADASSAQDGDEGGLTGSVSFEGNLDDLGIAIPAFATDRDVATP
ANAQGTGALGAELARVISNNLRNNGLFKPSGPDGLPRPTFPQITDPQWAVWSNRGAEMLVHGYVRARGDGNLVVGCYLYD
MALRDELVRSGWVVPPADWRRAAHKCSDLVYSRLTGESPFFDSRIAYIAETGPKDNRTKRLAIMDSDGANHRFITTGRAT
ALTPRYSPDYRRLLYLSYVDGNPRIYIYDIGTGRQQLVTESTNPTFAPRWSPDGRWILYSMAVNGNTDIYRVSAEGGPSV
RLTTEPGIDVGGSYSPDGSQIVFESDRSGSQQVYIMNSDGTNQRRISFFGGRAATPEWSPRGDQIAFTYIPGDFNIAVMS
PNGRNFRKLSNGWQDEAPTWAPNGRIIQFFRTERNTGRTALYQVDLTGDNLRRLNTPVDASDPAWGPILP

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=396, Percent_Identity=27.7777777777778, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 51219; Mature: 51087

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGTLLGAFALVGLITPLAAQDLASPPPRGGAVEQVAADASSAQDGDEGGLTGSVSFEG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECC
NLDDLGIAIPAFATDRDVATPANAQGTGALGAELARVISNNLRNNGLFKPSGPDGLPRPT
CCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCC
FPQITDPQWAVWSNRGAEMLVHGYVRARGDGNLVVGCYLYDMALRDELVRSGWVVPPADW
CCCCCCCCEEEECCCCCEEEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHCCCCCCCHHH
RRAAHKCSDLVYSRLTGESPFFDSRIAYIAETGPKDNRTKRLAIMDSDGANHRFITTGRA
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCC
TALTPRYSPDYRRLLYLSYVDGNPRIYIYDIGTGRQQLVTESTNPTFAPRWSPDGRWILY
EEECCCCCCCCEEEEEEEEECCCCEEEEEECCCCCHHEEECCCCCCCCCCCCCCCCEEEE
SMAVNGNTDIYRVSAEGGPSVRLTTEPGIDVGGSYSPDGSQIVFESDRSGSQQVYIMNSD
EEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCC
GTNQRRISFFGGRAATPEWSPRGDQIAFTYIPGDFNIAVMSPNGRNFRKLSNGWQDEAPT
CCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHCCCCCCCCC
WAPNGRIIQFFRTERNTGRTALYQVDLTGDNLRRLNTPVDASDPAWGPILP
CCCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRGTLLGAFALVGLITPLAAQDLASPPPRGGAVEQVAADASSAQDGDEGGLTGSVSFEG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECC
NLDDLGIAIPAFATDRDVATPANAQGTGALGAELARVISNNLRNNGLFKPSGPDGLPRPT
CCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCC
FPQITDPQWAVWSNRGAEMLVHGYVRARGDGNLVVGCYLYDMALRDELVRSGWVVPPADW
CCCCCCCCEEEECCCCCEEEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHCCCCCCCHHH
RRAAHKCSDLVYSRLTGESPFFDSRIAYIAETGPKDNRTKRLAIMDSDGANHRFITTGRA
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCC
TALTPRYSPDYRRLLYLSYVDGNPRIYIYDIGTGRQQLVTESTNPTFAPRWSPDGRWILY
EEECCCCCCCCEEEEEEEEECCCCEEEEEECCCCCHHEEECCCCCCCCCCCCCCCCEEEE
SMAVNGNTDIYRVSAEGGPSVRLTTEPGIDVGGSYSPDGSQIVFESDRSGSQQVYIMNSD
EEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCC
GTNQRRISFFGGRAATPEWSPRGDQIAFTYIPGDFNIAVMSPNGRNFRKLSNGWQDEAPT
CCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHCCCCCCCCC
WAPNGRIIQFFRTERNTGRTALYQVDLTGDNLRRLNTPVDASDPAWGPILP
CCCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA