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The map label for this gene is ptrB [C]

Identifier: 85374586

GI number: 85374586

Start: 1791815

End: 1793986

Strand: Direct

Name: ptrB [C]

Synonym: ELI_08795

Alternate gene names: 85374586

Gene position: 1791815-1793986 (Clockwise)

Preceding gene: 85374585

Following gene: 85374591

Centisome position: 58.7

GC content: 62.15

Gene sequence:

>2172_bases
ATGAAATTCACTTCCATGCAGGCGCTTGCCGCCGGCCTTGCGCTGGCTACCGCCGTTTCACCGCTCGACGCACAGGATTC
CAACACAGTGCTAGAACCAGAATACGAGGCCGAGAACGATCCGTTCATCTGGCTGGAGGAAACCCGCAGCGATCGCGCGC
TCGAATGGGTGCGCGGCGAGAATGCCAAGACCGAAGAGGCGCTGCAGAGCGATCCCCGGTTCGAAGAACTCAAGGCCGAA
GCGCTGGCTATCTATAACGCCGACGACCGTGTCCCGAGCATCAGCTTCACGCATTATGGCCGCGTCAACTTCTGGCAGGA
TGCCGACAACCCCAAGGGCATCCTTCGCCGCACCACGATGGAAAGCTGGCGGACCGACGATCCGCAATGGGAGACCATCC
TCGATATCGACGCGCTGGCGGCGGCCGAGGGCAAGGAATGGGTCTATGGCGGGATGACCTGCCTGCCGCCCGACGGCACG
CGCTGCATGGTCTACCTGTCTGACGGGGGCAAGGATGCGCGTGTCGTGCGCGAATTCGATCTTGAAACGCTCGATTTCGT
CGAAGGCGGTTTCGAACTCGAGGAAAGCCAGGGCAGCGTCAGCTGGCTCGACCGTGATACACTGCTGGTGAGCCGCAACT
TCAACGAAGATACGACGACCGAGAGCTTTTACCCCTTCACCACCCGGCTGTGGAAACGCGGCACGGCGATCGAAGATGCA
CCGGAGATCTTCCGCGGCGAAAAGAGCGACGTGTCGTCAGGTGCCTACCTGCTGCGCGATGGTGAAGCGACAATCCATGG
CCGCATGGCTTACCGCGGCCTTTCCTTCCACGAACGCACCTATTTCTTCGAGAAGGACGGCGAGTGGCTGCAACTCGACA
TTCCGAAGAAGGCGAATCCCTACGGCATTATCGACGGGCAGATCCTGTTTTCGACCGATGTCGACTGGACCAGGGGCGAG
CAGACCTTCCCCGCCGACGCCATTGTGGCGGCTGACCTGGAGGAGTGGAAGGCCGATCCCAATGGCGCCGACTTGACGCT
GGTCTGGGCTCCAGGCGAACGCCAGACCAAGCGTGGCGGCGCGAACACCAAGAGCAGTATGTATGTGAATCTGCTCGACA
ATGTGCGCGGCAAGGTTCTCAAGTTCGACTACGAGGACGGCGCGTGGGTCAGCCGCGAAATCGACCTGCCCGACAATGCC
ACCACCGGCATCACCACCGCTTCGGACGAGACCGACGAAATCATGTTCGCGGCGACCGATTTCCTCACCCCGACGCGCTG
GTTCTATGCCGAAGACGGTGTAACGCTCGAACAGGTCAAGGAAAGCCCGAGCCGCTTCGATGCCGAGGGCATGGACATCG
AGCAGTACGAAGCGACCAGCAAGGACGGGACCAAAATTCCTTATTTCATCGTCAAGCCTGAGGGCATGGTGATGGACGGC
TCAACCCCGACCCTGCTCACCGGTTACGGAGGTTTTCAGGTCCCGCGCCTGCCGGGCTATCTCGATTCGACCGGCAAGCT
GTGGCTGGAGCGCGGCGGGGCCTATGTGCTCGGCAACATGCGCGGCGGCGGAGAGTTCGGGCCGCAATGGCACCAGACTG
CGATCCGCGAGAACAAGCAGCGGACCTGGGATGATTTCATCGCCATCGCCGAAGATATCCAGGCGCGCGGCTTCACCAGC
GCCGAACATCTCGGCATCCAGGGTGGCTCGCAGGGCGGCCTGCTGGTCGGCACCGCCTTCACCCAGCGCCCGGACCTGTT
CAACGCGGCGATCGTGCAGATCCCGCTGTTCGACATGCTGCGCTATCACCTGATCGGGCGCGGTGCCTCGTGGATCGGCG
AATATGGCGACCCGCGCATCCCCGAACAGCGCGAATGGATCGAGGGCTATTCACCCTACCAGAAACTGACCAAGGACAAG
GACTTCCCGCGCATCTATTACGTCACCTCGACTGCCGATGACCGAACCCACCCGAGCCACGGCCGCAAGGCTGCCGCGCG
GATGGCGGCTAACGGGCAGGACTACCTCTATTACGAGGACATGACCGGCGGCCATTCGGGCGGCGTCGACAACGAACAGC
GCGCCAAGCTGCAAGCGATGCAGTGGGTGTACCTGATGCAGCAGTTGATGGATGCGCGTGCAGAGGGGGCGGAAGCGGCG
GGCGCCGAATAG

Upstream 100 bases:

>100_bases
TTTCGTTCGACGAACCGCCGACCTTGGCCTTCCGAACCCCTTGTTTTCGCTGCTCAGCTTGGCATGGTGCGCATCTGAAC
TTCATCCAGGGGACGCATCC

Downstream 100 bases:

>100_bases
AAGCAGCGGCAATCGACGACGGATAGACATGGGCGGCTCCGCGTGAGCCGCCCTTTTCTATTCGTTCGTGGAAACTTCGA
TCATGACTTCGTTGCGCCGC

Product: prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MKFTSMQALAAGLALATAVSPLDAQDSNTVLEPEYEAENDPFIWLEETRSDRALEWVRGENAKTEEALQSDPRFEELKAE
ALAIYNADDRVPSISFTHYGRVNFWQDADNPKGILRRTTMESWRTDDPQWETILDIDALAAAEGKEWVYGGMTCLPPDGT
RCMVYLSDGGKDARVVREFDLETLDFVEGGFELEESQGSVSWLDRDTLLVSRNFNEDTTTESFYPFTTRLWKRGTAIEDA
PEIFRGEKSDVSSGAYLLRDGEATIHGRMAYRGLSFHERTYFFEKDGEWLQLDIPKKANPYGIIDGQILFSTDVDWTRGE
QTFPADAIVAADLEEWKADPNGADLTLVWAPGERQTKRGGANTKSSMYVNLLDNVRGKVLKFDYEDGAWVSREIDLPDNA
TTGITTASDETDEIMFAATDFLTPTRWFYAEDGVTLEQVKESPSRFDAEGMDIEQYEATSKDGTKIPYFIVKPEGMVMDG
STPTLLTGYGGFQVPRLPGYLDSTGKLWLERGGAYVLGNMRGGGEFGPQWHQTAIRENKQRTWDDFIAIAEDIQARGFTS
AEHLGIQGGSQGGLLVGTAFTQRPDLFNAAIVQIPLFDMLRYHLIGRGASWIGEYGDPRIPEQREWIEGYSPYQKLTKDK
DFPRIYYVTSTADDRTHPSHGRKAAARMAANGQDYLYYEDMTGGHSGGVDNEQRAKLQAMQWVYLMQQLMDARAEGAEAA
GAE

Sequences:

>Translated_723_residues
MKFTSMQALAAGLALATAVSPLDAQDSNTVLEPEYEAENDPFIWLEETRSDRALEWVRGENAKTEEALQSDPRFEELKAE
ALAIYNADDRVPSISFTHYGRVNFWQDADNPKGILRRTTMESWRTDDPQWETILDIDALAAAEGKEWVYGGMTCLPPDGT
RCMVYLSDGGKDARVVREFDLETLDFVEGGFELEESQGSVSWLDRDTLLVSRNFNEDTTTESFYPFTTRLWKRGTAIEDA
PEIFRGEKSDVSSGAYLLRDGEATIHGRMAYRGLSFHERTYFFEKDGEWLQLDIPKKANPYGIIDGQILFSTDVDWTRGE
QTFPADAIVAADLEEWKADPNGADLTLVWAPGERQTKRGGANTKSSMYVNLLDNVRGKVLKFDYEDGAWVSREIDLPDNA
TTGITTASDETDEIMFAATDFLTPTRWFYAEDGVTLEQVKESPSRFDAEGMDIEQYEATSKDGTKIPYFIVKPEGMVMDG
STPTLLTGYGGFQVPRLPGYLDSTGKLWLERGGAYVLGNMRGGGEFGPQWHQTAIRENKQRTWDDFIAIAEDIQARGFTS
AEHLGIQGGSQGGLLVGTAFTQRPDLFNAAIVQIPLFDMLRYHLIGRGASWIGEYGDPRIPEQREWIEGYSPYQKLTKDK
DFPRIYYVTSTADDRTHPSHGRKAAARMAANGQDYLYYEDMTGGHSGGVDNEQRAKLQAMQWVYLMQQLMDARAEGAEAA
GAE
>Mature_723_residues
MKFTSMQALAAGLALATAVSPLDAQDSNTVLEPEYEAENDPFIWLEETRSDRALEWVRGENAKTEEALQSDPRFEELKAE
ALAIYNADDRVPSISFTHYGRVNFWQDADNPKGILRRTTMESWRTDDPQWETILDIDALAAAEGKEWVYGGMTCLPPDGT
RCMVYLSDGGKDARVVREFDLETLDFVEGGFELEESQGSVSWLDRDTLLVSRNFNEDTTTESFYPFTTRLWKRGTAIEDA
PEIFRGEKSDVSSGAYLLRDGEATIHGRMAYRGLSFHERTYFFEKDGEWLQLDIPKKANPYGIIDGQILFSTDVDWTRGE
QTFPADAIVAADLEEWKADPNGADLTLVWAPGERQTKRGGANTKSSMYVNLLDNVRGKVLKFDYEDGAWVSREIDLPDNA
TTGITTASDETDEIMFAATDFLTPTRWFYAEDGVTLEQVKESPSRFDAEGMDIEQYEATSKDGTKIPYFIVKPEGMVMDG
STPTLLTGYGGFQVPRLPGYLDSTGKLWLERGGAYVLGNMRGGGEFGPQWHQTAIRENKQRTWDDFIAIAEDIQARGFTS
AEHLGIQGGSQGGLLVGTAFTQRPDLFNAAIVQIPLFDMLRYHLIGRGASWIGEYGDPRIPEQREWIEGYSPYQKLTKDK
DFPRIYYVTSTADDRTHPSHGRKAAARMAANGQDYLYYEDMTGGHSGGVDNEQRAKLQAMQWVYLMQQLMDARAEGAEAA
GAE

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=370, Percent_Identity=33.2432432432432, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI284172438, Length=272, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI284172431, Length=272, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI284172420, Length=272, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI284172413, Length=272, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI70778815, Length=272, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI108860686, Length=271, Percent_Identity=23.6162361623616, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788150, Length=267, Percent_Identity=28.4644194756554, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583414, Length=362, Percent_Identity=31.4917127071823, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI221510989, Length=287, Percent_Identity=32.404181184669, Blast_Score=160, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 81076; Mature: 81076

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTSMQALAAGLALATAVSPLDAQDSNTVLEPEYEAENDPFIWLEETRSDRALEWVRGE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCHHHHHCCC
NAKTEEALQSDPRFEELKAEALAIYNADDRVPSISFTHYGRVNFWQDADNPKGILRRTTM
CCCHHHHHHCCCCHHHHCCEEEEEECCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHH
ESWRTDDPQWETILDIDALAAAEGKEWVYGGMTCLPPDGTRCMVYLSDGGKDARVVREFD
HHHCCCCCCCEEEEEHHHHHHCCCCCEEECCEEEECCCCCEEEEEECCCCCCCCCEECCC
LETLDFVEGGFELEESQGSVSWLDRDTLLVSRNFNEDTTTESFYPFTTRLWKRGTAIEDA
CCHHHHHCCCCEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCC
PEIFRGEKSDVSSGAYLLRDGEATIHGRMAYRGLSFHERTYFFEKDGEWLQLDIPKKANP
HHHHCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCC
YGIIDGQILFSTDVDWTRGEQTFPADAIVAADLEEWKADPNGADLTLVWAPGERQTKRGG
CEEECCEEEEECCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCEEEEEECCCCCHHHCCC
ANTKSSMYVNLLDNVRGKVLKFDYEDGAWVSREIDLPDNATTGITTASDETDEIMFAATD
CCCCCEEEEEEHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCEEECCCCCCCEEEEEHH
FLTPTRWFYAEDGVTLEQVKESPSRFDAEGMDIEQYEATSKDGTKIPYFIVKPEGMVMDG
CCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCEEECC
STPTLLTGYGGFQVPRLPGYLDSTGKLWLERGGAYVLGNMRGGGEFGPQWHQTAIRENKQ
CCCEEEECCCCEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCC
RTWDDFIAIAEDIQARGFTSAEHLGIQGGSQGGLLVGTAFTQRPDLFNAAIVQIPLFDML
CCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHH
RYHLIGRGASWIGEYGDPRIPEQREWIEGYSPYQKLTKDKDFPRIYYVTSTADDRTHPSH
HHHHHCCCHHHHCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCC
GRKAAARMAANGQDYLYYEDMTGGHSGGVDNEQRAKLQAMQWVYLMQQLMDARAEGAEAA
CHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAE
CCC
>Mature Secondary Structure
MKFTSMQALAAGLALATAVSPLDAQDSNTVLEPEYEAENDPFIWLEETRSDRALEWVRGE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCHHHHHCCC
NAKTEEALQSDPRFEELKAEALAIYNADDRVPSISFTHYGRVNFWQDADNPKGILRRTTM
CCCHHHHHHCCCCHHHHCCEEEEEECCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHH
ESWRTDDPQWETILDIDALAAAEGKEWVYGGMTCLPPDGTRCMVYLSDGGKDARVVREFD
HHHCCCCCCCEEEEEHHHHHHCCCCCEEECCEEEECCCCCEEEEEECCCCCCCCCEECCC
LETLDFVEGGFELEESQGSVSWLDRDTLLVSRNFNEDTTTESFYPFTTRLWKRGTAIEDA
CCHHHHHCCCCEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCC
PEIFRGEKSDVSSGAYLLRDGEATIHGRMAYRGLSFHERTYFFEKDGEWLQLDIPKKANP
HHHHCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCC
YGIIDGQILFSTDVDWTRGEQTFPADAIVAADLEEWKADPNGADLTLVWAPGERQTKRGG
CEEECCEEEEECCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCEEEEEECCCCCHHHCCC
ANTKSSMYVNLLDNVRGKVLKFDYEDGAWVSREIDLPDNATTGITTASDETDEIMFAATD
CCCCCEEEEEEHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCEEECCCCCCCEEEEEHH
FLTPTRWFYAEDGVTLEQVKESPSRFDAEGMDIEQYEATSKDGTKIPYFIVKPEGMVMDG
CCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCEEECC
STPTLLTGYGGFQVPRLPGYLDSTGKLWLERGGAYVLGNMRGGGEFGPQWHQTAIRENKQ
CCCEEEECCCCEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCC
RTWDDFIAIAEDIQARGFTSAEHLGIQGGSQGGLLVGTAFTQRPDLFNAAIVQIPLFDML
CCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHH
RYHLIGRGASWIGEYGDPRIPEQREWIEGYSPYQKLTKDKDFPRIYYVTSTADDRTHPSH
HHHHHCCCHHHHCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCC
GRKAAARMAANGQDYLYYEDMTGGHSGGVDNEQRAKLQAMQWVYLMQQLMDARAEGAEAA
CHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]