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The map label for this gene is rsmD [H]

Identifier: 85374351

GI number: 85374351

Start: 1577918

End: 1578466

Strand: Direct

Name: rsmD [H]

Synonym: ELI_07620

Alternate gene names: 85374351

Gene position: 1577918-1578466 (Clockwise)

Preceding gene: 85374350

Following gene: 85374353

Centisome position: 51.69

GC content: 68.85

Gene sequence:

>549_bases
GTGAGGATCATCGCCGGCGAATGGCGCGGGCGGAAACTGGTCGCACCGAGGGGCGACGGCACCCGCCCGACCGCAGACCG
GACGCGCGAGACTTTGTTCTCGATGCTCACCAGCCGCCTGGGGAGTTTCGAAGGACTGAGCGCGGTCGATCTGTTCGCCG
GCTCCGGTGCGCTCGGGCTGGAGGCGCTGTCGCGCGGCGCAGCGCATTGCCTGTTCGTGGAGCAGGACAAGCCCGCGCTC
GATGTGATCCGCGCAAATATCGTTGCTCTGAATGCCCGCGCGAGGGCGACCGTATCGGCCGGATCGGTGATGGCGCTCGG
CCCGGCCAAAGCGCCGCACGACCTGGTCCTCGCCGACCCACCTTACGACTCCGGCGCCGGGGCGGTAGCGCTCGACCGGT
TGCTGCGGCTCGGCTGGATCGGGCCGCAGACGTGGATCGCAGTCGAGACCGCCGCGCAGGAAGATGTCGCGGTCAAGGGC
CTCGCAATCGAGACCGAACGCAAGGTCGGGAAGGCGAAGCTGACCATTCTCCGCCTTCCCGCCGGCTAA

Upstream 100 bases:

>100_bases
ATGGCCCGTTCCAGCTCGGCGACCTGCCGCGCGGCCAAGCCGTCGAAATCCGCAAGGGCGATCTCGGCCGGTTCCTGAGC
ACGCTCAAGAAGGAGGGCGC

Downstream 100 bases:

>100_bases
CCCGTTCCGACTGTCATTCTGCCTTGTCGTCGGGGAGAGGTGGGGTGTCGCCGGGATCGGTCTCGGCCTCGTCTGCCGGG
GTGCTGCCCGCGGGCGGATC

Product: N6-adenine-specific methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MRIIAGEWRGRKLVAPRGDGTRPTADRTRETLFSMLTSRLGSFEGLSAVDLFAGSGALGLEALSRGAAHCLFVEQDKPAL
DVIRANIVALNARARATVSAGSVMALGPAKAPHDLVLADPPYDSGAGAVALDRLLRLGWIGPQTWIAVETAAQEDVAVKG
LAIETERKVGKAKLTILRLPAG

Sequences:

>Translated_182_residues
MRIIAGEWRGRKLVAPRGDGTRPTADRTRETLFSMLTSRLGSFEGLSAVDLFAGSGALGLEALSRGAAHCLFVEQDKPAL
DVIRANIVALNARARATVSAGSVMALGPAKAPHDLVLADPPYDSGAGAVALDRLLRLGWIGPQTWIAVETAAQEDVAVKG
LAIETERKVGKAKLTILRLPAG
>Mature_182_residues
MRIIAGEWRGRKLVAPRGDGTRPTADRTRETLFSMLTSRLGSFEGLSAVDLFAGSGALGLEALSRGAAHCLFVEQDKPAL
DVIRANIVALNARARATVSAGSVMALGPAKAPHDLVLADPPYDSGAGAVALDRLLRLGWIGPQTWIAVETAAQEDVAVKG
LAIETERKVGKAKLTILRLPAG

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=154, Percent_Identity=39.6103896103896, Blast_Score=107, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 19103; Mature: 19103

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIAGEWRGRKLVAPRGDGTRPTADRTRETLFSMLTSRLGSFEGLSAVDLFAGSGALGL
CEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
EALSRGAAHCLFVEQDKPALDVIRANIVALNARARATVSAGSVMALGPAKAPHDLVLADP
HHHHCCCEEEEEEECCCCHHHHHEEEEEEECCCCEEEECCCCEEEECCCCCCCCEEEECC
PYDSGAGAVALDRLLRLGWIGPQTWIAVETAAQEDVAVKGLAIETERKVGKAKLTILRLP
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEECCCCCCCEEEEEEEEC
AG
CC
>Mature Secondary Structure
MRIIAGEWRGRKLVAPRGDGTRPTADRTRETLFSMLTSRLGSFEGLSAVDLFAGSGALGL
CEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
EALSRGAAHCLFVEQDKPALDVIRANIVALNARARATVSAGSVMALGPAKAPHDLVLADP
HHHHCCCEEEEEEECCCCHHHHHEEEEEEECCCCEEEECCCCEEEECCCCCCCCEEEECC
PYDSGAGAVALDRLLRLGWIGPQTWIAVETAAQEDVAVKGLAIETERKVGKAKLTILRLP
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEECCCCCCCEEEEEEEEC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]