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The map label for this gene is purB [H]

Identifier: 85374340

GI number: 85374340

Start: 1566603

End: 1567913

Strand: Direct

Name: purB [H]

Synonym: ELI_07565

Alternate gene names: 85374340

Gene position: 1566603-1567913 (Clockwise)

Preceding gene: 85374339

Following gene: 85374341

Centisome position: 51.32

GC content: 63.31

Gene sequence:

>1311_bases
ATGGTCCCCCGCTATGCCCGCCCCGCCATGACCGCCCTGTGGGAGCCGGAGGCGAAATATCGCATCTGGTTCGAGATCGA
GGCGCATGCGACCGAGAAACTAGGCGAGCTCGGAGTGGTGCCGGAAAGCGCGGCCAAGGCCTTGTGGGACTGGTGGGCGA
CCGATCCCAAGATCGACGTCGAGGCGATCGACGCTATCGAAGCGGTGACCAAGCACGATGTCATCGCCTTTCTCACCTGG
GTCGCCGAGAATGTCGGCGAGGAAGCACGCTTCATGCATCAGGGCATGACCAGCAGCGATGTGCTCGACACGACGCTGGC
GGTGCAGCTGGCGCGATCGACGGACATTCTGCTCGAAGACCTCGATGCGCTACTCGCCGCGATCAAGCGCCGCGCGGAAG
AGCAAAAATACACGCCGACGATCGGGCGCAGCCACGGCATCCACGCCGAACCGGTGACCTTCGGCCTCAAGCTCGCGCAG
GCCTATGCCGAGTTCGATCGCTGCAAGACCCGCCTGGTCGCCGCGCGCGAGGAAATTGCCACCTGTGCCATCAGCGGCGC
CGTCGGGACTTTCGCCAACATCGATCCTTCGGTCGAAGAGCATGTCGCCGACAAGCTCGGCCTCAAGCCCGAGCCGGTTA
GCACGCAGGTGATCCCGCGCGACCGCCATGCGATGTTCTTCTCCACCCTCGCCGTCATTGCCGGGTCGATCGAGCGGCTC
GCGGTCGAGATTCGCCATCTCCAGCGCACCGAAGTGCTGGAAGCGGAGGAATATTTCTCACCCGGCCAGAAGGGTTCGAG
CGCGATGCCGCACAAACGCAACCCGATCCTGACGGAAAACCTTACCGGCCAGGCCCGCATGATCCGTGCCTATGCCATGC
CGGCGCTCGAGAATGTCGCGCTGTGGCATGAGCGCGATATCTCGCACTCCTCGGTCGAGCGCTTCATCGGCCCCGATGCC
TGCATCACGCTCGATTTCGCGCTCGCGCGGCTGACCGGGGTGATCGACAAGCTGCTGGTCTATCCCGAACGCATGCAGGC
CAACATGGACCGGATGGGAGGCCTGATCCATTCGCAGCGCGTGTTGCTTGCGCTCACCCAGAACGGCGTCAGCCGCGAGG
ATGCCTATCGCCTCGTCCAGCGCAATGCGATGAAGGTATGGGAATCGGACGGCCAGCTGATGCTGCTCGACCTGCTCAAA
CAGGACGCGGAAGTGACCGCCGCCCTGTCCGAAAGCGAGCTCGAAGAGCGCTTCGATCTCGAATACCATTTCAAGCACGT
CGACACGATCTTCAACCGCGTCTTCGGCTAG

Upstream 100 bases:

>100_bases
CGAGGGGCACGTGAGCCTCAGGGCGAAAGGCTTGATCTGAGCGACACCGCAGCCTAGCCGCCGCGCGACAGTTTCAAACC
CTTTTTCCCGGAGTCGACCG

Downstream 100 bases:

>100_bases
AGAGTGCGCAGTATCTGGACGCGGCCCTTTCTGCGCCCTAGCATCATGATGAAATAAGGGAGGGGTTCGCCTTGCAAATC
GTCCGCACAATCGTCTGGGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MVPRYARPAMTALWEPEAKYRIWFEIEAHATEKLGELGVVPESAAKALWDWWATDPKIDVEAIDAIEAVTKHDVIAFLTW
VAENVGEEARFMHQGMTSSDVLDTTLAVQLARSTDILLEDLDALLAAIKRRAEEQKYTPTIGRSHGIHAEPVTFGLKLAQ
AYAEFDRCKTRLVAAREEIATCAISGAVGTFANIDPSVEEHVADKLGLKPEPVSTQVIPRDRHAMFFSTLAVIAGSIERL
AVEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPILTENLTGQARMIRAYAMPALENVALWHERDISHSSVERFIGPDA
CITLDFALARLTGVIDKLLVYPERMQANMDRMGGLIHSQRVLLALTQNGVSREDAYRLVQRNAMKVWESDGQLMLLDLLK
QDAEVTAALSESELEERFDLEYHFKHVDTIFNRVFG

Sequences:

>Translated_436_residues
MVPRYARPAMTALWEPEAKYRIWFEIEAHATEKLGELGVVPESAAKALWDWWATDPKIDVEAIDAIEAVTKHDVIAFLTW
VAENVGEEARFMHQGMTSSDVLDTTLAVQLARSTDILLEDLDALLAAIKRRAEEQKYTPTIGRSHGIHAEPVTFGLKLAQ
AYAEFDRCKTRLVAAREEIATCAISGAVGTFANIDPSVEEHVADKLGLKPEPVSTQVIPRDRHAMFFSTLAVIAGSIERL
AVEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPILTENLTGQARMIRAYAMPALENVALWHERDISHSSVERFIGPDA
CITLDFALARLTGVIDKLLVYPERMQANMDRMGGLIHSQRVLLALTQNGVSREDAYRLVQRNAMKVWESDGQLMLLDLLK
QDAEVTAALSESELEERFDLEYHFKHVDTIFNRVFG
>Mature_436_residues
MVPRYARPAMTALWEPEAKYRIWFEIEAHATEKLGELGVVPESAAKALWDWWATDPKIDVEAIDAIEAVTKHDVIAFLTW
VAENVGEEARFMHQGMTSSDVLDTTLAVQLARSTDILLEDLDALLAAIKRRAEEQKYTPTIGRSHGIHAEPVTFGLKLAQ
AYAEFDRCKTRLVAAREEIATCAISGAVGTFANIDPSVEEHVADKLGLKPEPVSTQVIPRDRHAMFFSTLAVIAGSIERL
AVEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPILTENLTGQARMIRAYAMPALENVALWHERDISHSSVERFIGPDA
CITLDFALARLTGVIDKLLVYPERMQANMDRMGGLIHSQRVLLALTQNGVSREDAYRLVQRNAMKVWESDGQLMLLDLLK
QDAEVTAALSESELEERFDLEYHFKHVDTIFNRVFG

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=409, Percent_Identity=26.8948655256724, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI183227688, Length=388, Percent_Identity=27.0618556701031, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787376, Length=299, Percent_Identity=29.4314381270903, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=405, Percent_Identity=24.9382716049383, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32564234, Length=375, Percent_Identity=26.4, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323391, Length=418, Percent_Identity=26.555023923445, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647570, Length=382, Percent_Identity=26.1780104712042, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48900; Mature: 48900

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRYARPAMTALWEPEAKYRIWFEIEAHATEKLGELGVVPESAAKALWDWWATDPKIDV
CCCCCCCCHHHHHCCCCCCEEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCH
EAIDAIEAVTKHDVIAFLTWVAENVGEEARFMHQGMTSSDVLDTTLAVQLARSTDILLED
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDALLAAIKRRAEEQKYTPTIGRSHGIHAEPVTFGLKLAQAYAEFDRCKTRLVAAREEIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCAISGAVGTFANIDPSVEEHVADKLGLKPEPVSTQVIPRDRHAMFFSTLAVIAGSIERL
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPILTENLTGQARMIRAYAMPALENVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LWHERDISHSSVERFIGPDACITLDFALARLTGVIDKLLVYPERMQANMDRMGGLIHSQR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHCCHHHHCC
VLLALTQNGVSREDAYRLVQRNAMKVWESDGQLMLLDLLKQDAEVTAALSESELEERFDL
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCH
EYHFKHVDTIFNRVFG
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVPRYARPAMTALWEPEAKYRIWFEIEAHATEKLGELGVVPESAAKALWDWWATDPKIDV
CCCCCCCCHHHHHCCCCCCEEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCH
EAIDAIEAVTKHDVIAFLTWVAENVGEEARFMHQGMTSSDVLDTTLAVQLARSTDILLED
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDALLAAIKRRAEEQKYTPTIGRSHGIHAEPVTFGLKLAQAYAEFDRCKTRLVAAREEIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCAISGAVGTFANIDPSVEEHVADKLGLKPEPVSTQVIPRDRHAMFFSTLAVIAGSIERL
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPILTENLTGQARMIRAYAMPALENVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LWHERDISHSSVERFIGPDACITLDFALARLTGVIDKLLVYPERMQANMDRMGGLIHSQR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHCCHHHHCC
VLLALTQNGVSREDAYRLVQRNAMKVWESDGQLMLLDLLKQDAEVTAALSESELEERFDL
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCH
EYHFKHVDTIFNRVFG
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571; 10673438 [H]