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The map label for this gene is gloB

Identifier: 85374329

GI number: 85374329

Start: 1557224

End: 1557979

Strand: Direct

Name: gloB

Synonym: ELI_07510

Alternate gene names: 85374329

Gene position: 1557224-1557979 (Clockwise)

Preceding gene: 85374328

Following gene: 85374330

Centisome position: 51.02

GC content: 61.9

Gene sequence:

>756_bases
ATGCTAGAGATCCATCAATTCCCCTGCCTGTCCGACAATTACGGCTTCCTGCTCCACGATCCCGACAGCGGAGAAACCGC
TGCGATCGATACGCCTGACGGAAAGGAATACCTGAAACAGGCCAAGGCCAAGGGCTGGACGATCACTCATATCTGGAACA
CCCACTGGCACCCGGACCATGCGGGCGGGAACAAGGATATTGTCGAGGCGACCGGTGCCAAGGTCATCGCGCCGCAGGAG
GTCGAGAAGCTGTCGGGGATCGACCGGGTCGTCGGTCACGGCGATACCGTCGACATCGGAGACTTCACCGCCGATGTCAT
CGATGTCAGCGGACATACCAACGGCCATATCGCCTACCACCTGCCCGAAGCGGGGATCGCCTTTGTTGGCGACAGTGTTT
TCGCGCTGGGCTGCGGGCGGATGTTCGAAGGCGAGCCGAAGCAGTTCTGGGACAGCCTCAGCCGCATCAAGGCCCTGCCG
CCCGAAACCATGCTCTATTGCGCGCACGAATATACCGCGGCGAACGCCAGGTTCGCGGTGCACGCCGATCCGGAAAACGA
TGCCCTCGTCGCCTATGTCGAGGAGATCACCGCCAAGCGCGAGCGCGACGAGCCCACCGTACCCACGCAGTTGAAGCGCG
AGCTGGCCACCAATCCCTTCCTGCGCGCCGACGACCCGGCGCTGGTAGCAAAATGGGGCGGTGATGCGCCGCATGAAACC
TTTGCCGCGTTAAGGGCGGGCAAGGACAATTTCTGA

Upstream 100 bases:

>100_bases
GATCCCAGCTTTCGCTGGGATGACGATGAGGATGAAAGGCCCGCCTTCCGATGGAGTGGCGGGCCTTTTCTATTGCGGGT
CAGCGATGTAGGCTCAGTGC

Downstream 100 bases:

>100_bases
CACCATGAAAGCGCTGCGCGTCCAGCACCTGTCGGACGACCTGTCCGGCGTGGAACTGGTCGACCTGCCGCAGCCCGAAC
GAAAGCAGGGCGAGGTGCTC

Product: hydroxyacylglutathione hydrolase, putative

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLEIHQFPCLSDNYGFLLHDPDSGETAAIDTPDGKEYLKQAKAKGWTITHIWNTHWHPDHAGGNKDIVEATGAKVIAPQE
VEKLSGIDRVVGHGDTVDIGDFTADVIDVSGHTNGHIAYHLPEAGIAFVGDSVFALGCGRMFEGEPKQFWDSLSRIKALP
PETMLYCAHEYTAANARFAVHADPENDALVAYVEEITAKRERDEPTVPTQLKRELATNPFLRADDPALVAKWGGDAPHET
FAALRAGKDNF

Sequences:

>Translated_251_residues
MLEIHQFPCLSDNYGFLLHDPDSGETAAIDTPDGKEYLKQAKAKGWTITHIWNTHWHPDHAGGNKDIVEATGAKVIAPQE
VEKLSGIDRVVGHGDTVDIGDFTADVIDVSGHTNGHIAYHLPEAGIAFVGDSVFALGCGRMFEGEPKQFWDSLSRIKALP
PETMLYCAHEYTAANARFAVHADPENDALVAYVEEITAKRERDEPTVPTQLKRELATNPFLRADDPALVAKWGGDAPHET
FAALRAGKDNF
>Mature_251_residues
MLEIHQFPCLSDNYGFLLHDPDSGETAAIDTPDGKEYLKQAKAKGWTITHIWNTHWHPDHAGGNKDIVEATGAKVIAPQE
VEKLSGIDRVVGHGDTVDIGDFTADVIDVSGHTNGHIAYHLPEAGIAFVGDSVFALGCGRMFEGEPKQFWDSLSRIKALP
PETMLYCAHEYTAANARFAVHADPENDALVAYVEEITAKRERDEPTVPTQLKRELATNPFLRADDPALVAKWGGDAPHET
FAALRAGKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=257, Percent_Identity=32.295719844358, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI94538322, Length=257, Percent_Identity=32.295719844358, Blast_Score=151, Evalue=4e-37,
Organism=Homo sapiens, GI14150041, Length=258, Percent_Identity=33.3333333333333, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI21703352, Length=232, Percent_Identity=31.8965517241379, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI116642887, Length=237, Percent_Identity=31.6455696202532, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI46361987, Length=204, Percent_Identity=31.3725490196078, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI41327741, Length=182, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786406, Length=255, Percent_Identity=35.6862745098039, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17536925, Length=261, Percent_Identity=31.8007662835249, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320478, Length=264, Percent_Identity=29.1666666666667, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324614, Length=247, Percent_Identity=28.3400809716599, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356335, Length=258, Percent_Identity=33.7209302325581, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24667703, Length=258, Percent_Identity=33.7209302325581, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24667711, Length=258, Percent_Identity=33.7209302325581, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI221330176, Length=214, Percent_Identity=29.9065420560748, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_ERYLH (Q2N9P7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458391.1
- ProteinModelPortal:   Q2N9P7
- SMR:   Q2N9P7
- STRING:   Q2N9P7
- GeneID:   3869572
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07510
- NMPDR:   fig|314225.3.peg.1541
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   Q2N9P7
- ProtClustDB:   CLSK753563
- BioCyc:   ELIT314225:ELI_07510-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27482; Mature: 27482

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIHQFPCLSDNYGFLLHDPDSGETAAIDTPDGKEYLKQAKAKGWTITHIWNTHWHPDH
CCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCC
AGGNKDIVEATGAKVIAPQEVEKLSGIDRVVGHGDTVDIGDFTADVIDVSGHTNGHIAYH
CCCCCCEEEECCCEEECHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCEEEEE
LPEAGIAFVGDSVFALGCGRMFEGEPKQFWDSLSRIKALPPETMLYCAHEYTAANARFAV
CCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEHHHCCCCCEEEE
HADPENDALVAYVEEITAKRERDEPTVPTQLKRELATNPFLRADDPALVAKWGGDAPHET
EECCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHH
FAALRAGKDNF
HHHHHCCCCCC
>Mature Secondary Structure
MLEIHQFPCLSDNYGFLLHDPDSGETAAIDTPDGKEYLKQAKAKGWTITHIWNTHWHPDH
CCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCC
AGGNKDIVEATGAKVIAPQEVEKLSGIDRVVGHGDTVDIGDFTADVIDVSGHTNGHIAYH
CCCCCCEEEECCCEEECHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCEEEEE
LPEAGIAFVGDSVFALGCGRMFEGEPKQFWDSLSRIKALPPETMLYCAHEYTAANARFAV
CCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEHHHCCCCCEEEE
HADPENDALVAYVEEITAKRERDEPTVPTQLKRELATNPFLRADDPALVAKWGGDAPHET
EECCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHH
FAALRAGKDNF
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA