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The map label for this gene is rotA [H]

Identifier: 85374254

GI number: 85374254

Start: 1478566

End: 1479207

Strand: Direct

Name: rotA [H]

Synonym: ELI_07135

Alternate gene names: 85374254

Gene position: 1478566-1479207 (Clockwise)

Preceding gene: 85374253

Following gene: 85374255

Centisome position: 48.44

GC content: 64.95

Gene sequence:

>642_bases
GTGCTGATGCTGCGTTCGTTTCTCGCCGGTGTGCTGGCTGTCCTCGCGCTCCCGCTTGCAGCGCAGGAAGCTGAAGCGCC
CGCTCCTTCCCTTCAGGAATACGAGACGGTCGATGTGGTGCTGGAAACCACCATGGGTGCGATCACTGTCGCGTTGGAAA
CTGAGCGTGCTCCGATCACGGCGGGCAATTTCCTGCGCTATGTCGACGAAGGCCGCTTCGACGGCACGGTCTTCTACCGC
GCCATGCATCTCGACTGGGAACCGCAGCCGAACGGTTTGATCCAGGGCGGCACCCAATGGGACCCGGACCGCGTTCTGCC
CGGCATCCCGCACGAGCCCACCACGCAAACCGGCCTCAGCCACACCCGCGGCGCGCTTTCCATGGCGATGGGCGAACCGG
GCACCGCCAACGGCGACTTTTCGATCATGGTGCAGGACCAAACCGGGATGGACGCCGACCCGGAAGCCGAGGACCCGGTA
TGGCAGAACGGATATGCCGTGTTCGGCTATGTCACCGACGGGATGGAGGTGGTCGAGGCCATCCACGCCGCCCCGCTCGA
CCCCGACAAGGGCGAAGGCTGGATGAAGGGCCAGATGCTGGCGGAACCGGTAAAGATCCTGACCGCGCGACGGGCCGAAT
AG

Upstream 100 bases:

>100_bases
AGGGCGCGTTGCAGCGGATGGCGGAAGCGATCGCCGCGACCGAAGCGACCGGCGGTACCGCGCCAAAAGGCTGGACCGAC
TTCGTGAAACAGGAAGGATA

Downstream 100 bases:

>100_bases
AAGGTCAGGAACGCGCGCCTTTCCGCTTGGTTGGTGGGGTATGGACACAGACCAGCAGAAGCAATCGATCCTTTCTTCGC
CGCAGCTGCGCCTCATCGGC

Product: peptidyl-prolyl cis-trans isomerase A

Products: NA

Alternate protein names: PPIase A; Cyclophilin A; Rotamase A [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLMLRSFLAGVLAVLALPLAAQEAEAPAPSLQEYETVDVVLETTMGAITVALETERAPITAGNFLRYVDEGRFDGTVFYR
AMHLDWEPQPNGLIQGGTQWDPDRVLPGIPHEPTTQTGLSHTRGALSMAMGEPGTANGDFSIMVQDQTGMDADPEAEDPV
WQNGYAVFGYVTDGMEVVEAIHAAPLDPDKGEGWMKGQMLAEPVKILTARRAE

Sequences:

>Translated_213_residues
MLMLRSFLAGVLAVLALPLAAQEAEAPAPSLQEYETVDVVLETTMGAITVALETERAPITAGNFLRYVDEGRFDGTVFYR
AMHLDWEPQPNGLIQGGTQWDPDRVLPGIPHEPTTQTGLSHTRGALSMAMGEPGTANGDFSIMVQDQTGMDADPEAEDPV
WQNGYAVFGYVTDGMEVVEAIHAAPLDPDKGEGWMKGQMLAEPVKILTARRAE
>Mature_213_residues
MLMLRSFLAGVLAVLALPLAAQEAEAPAPSLQEYETVDVVLETTMGAITVALETERAPITAGNFLRYVDEGRFDGTVFYR
AMHLDWEPQPNGLIQGGTQWDPDRVLPGIPHEPTTQTGLSHTRGALSMAMGEPGTANGDFSIMVQDQTGMDADPEAEDPV
WQNGYAVFGYVTDGMEVVEAIHAAPLDPDKGEGWMKGQMLAEPVKILTARRAE

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789763, Length=212, Percent_Identity=32.0754716981132, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786736, Length=152, Percent_Identity=34.2105263157895, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 23027; Mature: 23027

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMLRSFLAGVLAVLALPLAAQEAEAPAPSLQEYETVDVVLETTMGAITVALETERAPIT
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCC
AGNFLRYVDEGRFDGTVFYRAMHLDWEPQPNGLIQGGTQWDPDRVLPGIPHEPTTQTGLS
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCH
HTRGALSMAMGEPGTANGDFSIMVQDQTGMDADPEAEDPVWQNGYAVFGYVTDGMEVVEA
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHH
IHAAPLDPDKGEGWMKGQMLAEPVKILTARRAE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLMLRSFLAGVLAVLALPLAAQEAEAPAPSLQEYETVDVVLETTMGAITVALETERAPIT
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCC
AGNFLRYVDEGRFDGTVFYRAMHLDWEPQPNGLIQGGTQWDPDRVLPGIPHEPTTQTGLS
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCH
HTRGALSMAMGEPGTANGDFSIMVQDQTGMDADPEAEDPVWQNGYAVFGYVTDGMEVVEA
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHH
IHAAPLDPDKGEGWMKGQMLAEPVKILTARRAE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9418240 [H]