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The map label for this gene is slt [H]

Identifier: 85374215

GI number: 85374215

Start: 1438997

End: 1440775

Strand: Direct

Name: slt [H]

Synonym: ELI_06940

Alternate gene names: 85374215

Gene position: 1438997-1440775 (Clockwise)

Preceding gene: 85374214

Following gene: 85374216

Centisome position: 47.14

GC content: 67.68

Gene sequence:

>1779_bases
ATGGTCCCCATTCGCGTGTTTCGTTCTCTGGTCGTAGCAGGCGTCCTCGCCGGTGCAGCCTTCGCCACGGCCATGCCCGC
CCCCGCCTTCGCCAAAGGTGCCGCCGAGTATTTCCGCGCGCGGGCGGAAAACAACAATGTCCCCACGCTGCTCAGCGACA
GCGAGAAAGCCTATTATGCGCAGCTTTTCGCGGCGATCGACGATGAGCGCTGGGATACGGTCGAAACGCTGATCGGGCAG
CGCAGCGAAGGCCCGCTGCACCAGGTTGCGCTGGCCGAATACTATCTCCACGCCAACAGTCCGCGGGTCGAACTGCCCCA
AATCCAGACGTGGCTCGGTCGGGGCACGCGCCTGCCGATGGCCGAGCAGATCGCCAATCTCGGCCTCAAGCGCGGGTTGA
TGTCGGCGCCCTACCTCCCGCAGGAACAGAGCTTCACCCGCCAGCCCTACATCACCAAGCGCATCGCACCGCGGAGCATC
GACGACGGCACCATGCCCGGTTCGATCCGTTCGGCGATCCTCGACCGGATCAAGAACGACGATCCCGACGGCGCGCGGCA
GCTGCTCGACGGGATCGACGCTTCGCTCAGCTCCGCCGCGCGGGCCGAATGGCGCAAGCGCGTGGCGTGGAGTTATTACA
TCGAGAACCAGGATGCCGCCGCGCTGGCCATGGCCAAGACCGTGAGCCTCGACGGCTCGGGTGCCTGGGTGGCGGAAGGC
GAATGGGTCGCCGGCCTTGCCGCGTGGCGGCTCGGCAATTGCGGGGAAGCCGCGGACGGGTTCCGCCGTGCAGCGGCGTG
GGCAATGAACCCCGAATTGGGTGCAGCCTCGCACTATTGGGCTTCGCGCGCGCTGGTCCGGTGCCGCGAGCCGGAAAAAG
CCGCCGAGCAATTGCGCGGTGCAGCGATGATGCACGAGACGCTCTACGGCATGCTCGCGCGCGAACAGCTCGGCCAGACC
CTGCCCGACGACGGCGAAACGCCCGACCTCTCGCCCGCCGACTGGCAGCGCTTGCGCGATAACGGCAATGTCCGCGCCGC
CGTGGCGCTGGCCGAGATCGGTCGCGACGATCTGTCCGACGAGATTCTCCGCCACGAAGCGCGCATCGGCGACCCGTCCG
AATATGACGCGCTGTCCCGCCTCGCGCGCGAACTCGGCCTGCCGGGCACGCAGCTGTGGATGGCGCAGAACACCCCGCGC
GGCGGGACCTACGAGCCTGCGCTCCGCTTCCCGGCACCGCGCTGGCAGCCGGTCGACGGCTGGAAGGTCGATCCGGCACT
CGCCTACGCACACACGTTGCAGGAATCGAATTTTCGTCCGCGCGTCGTCAGCCCGGCGGGCGCGCGCGGCCTGATGCAGA
TCATGCCGGCCGCGGCGCGCGATCACGAGGGCACGATCGGCGTCTCCGGCACCGCCCGCAACCTCTCGCGCCCGGAAGTC
AATCTCGCCTTCGGCCAGAGACACTTGCAGATGCTGCGCGACAGCGGCGCGACCGGCGGCCTGCTGCCCAAGATCATGGC
TGCTTACAATGCAGGGCTCACGCCGATCACCCGCTGGAACAGCGAAGTGCGCGACCAGGGCGACGCGCTGCTCTACATGG
AAAGCATCCCCTATTGGGAAACGCGCGGCTATGTCGCCATCGTGATGCGCAATTACTGGATGTACGAGCGCCAGGCCGAT
GCCGACAGCTCCAGCCGCAAGGCGTTGGCAGAGGGCAAGTGGCCGACTTTCCCCGAGGTCAGCGGCGCAACCGGTGTGCG
GATGGCCTCCGCCAACTGA

Upstream 100 bases:

>100_bases
TTCCGCTGTTGCCAGCGCGCGATGTCGCCAATCTGGGGCGCCGCCCCGGTTTTAGGGCTCGCTTCTGGCAACAATGGTTG
GATTTTTCAGGTAGCTAGGC

Downstream 100 bases:

>100_bases
TGGGAATCGACAAAAGTCTCGACTTCAAATCGGTGCGCATTGCACTGCTGACGATCTCCGACACGCGCACTTTCGACGAT
GACACATCGGGCGACATCCT

Product: lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MVPIRVFRSLVVAGVLAGAAFATAMPAPAFAKGAAEYFRARAENNNVPTLLSDSEKAYYAQLFAAIDDERWDTVETLIGQ
RSEGPLHQVALAEYYLHANSPRVELPQIQTWLGRGTRLPMAEQIANLGLKRGLMSAPYLPQEQSFTRQPYITKRIAPRSI
DDGTMPGSIRSAILDRIKNDDPDGARQLLDGIDASLSSAARAEWRKRVAWSYYIENQDAAALAMAKTVSLDGSGAWVAEG
EWVAGLAAWRLGNCGEAADGFRRAAAWAMNPELGAASHYWASRALVRCREPEKAAEQLRGAAMMHETLYGMLAREQLGQT
LPDDGETPDLSPADWQRLRDNGNVRAAVALAEIGRDDLSDEILRHEARIGDPSEYDALSRLARELGLPGTQLWMAQNTPR
GGTYEPALRFPAPRWQPVDGWKVDPALAYAHTLQESNFRPRVVSPAGARGLMQIMPAAARDHEGTIGVSGTARNLSRPEV
NLAFGQRHLQMLRDSGATGGLLPKIMAAYNAGLTPITRWNSEVRDQGDALLYMESIPYWETRGYVAIVMRNYWMYERQAD
ADSSSRKALAEGKWPTFPEVSGATGVRMASAN

Sequences:

>Translated_592_residues
MVPIRVFRSLVVAGVLAGAAFATAMPAPAFAKGAAEYFRARAENNNVPTLLSDSEKAYYAQLFAAIDDERWDTVETLIGQ
RSEGPLHQVALAEYYLHANSPRVELPQIQTWLGRGTRLPMAEQIANLGLKRGLMSAPYLPQEQSFTRQPYITKRIAPRSI
DDGTMPGSIRSAILDRIKNDDPDGARQLLDGIDASLSSAARAEWRKRVAWSYYIENQDAAALAMAKTVSLDGSGAWVAEG
EWVAGLAAWRLGNCGEAADGFRRAAAWAMNPELGAASHYWASRALVRCREPEKAAEQLRGAAMMHETLYGMLAREQLGQT
LPDDGETPDLSPADWQRLRDNGNVRAAVALAEIGRDDLSDEILRHEARIGDPSEYDALSRLARELGLPGTQLWMAQNTPR
GGTYEPALRFPAPRWQPVDGWKVDPALAYAHTLQESNFRPRVVSPAGARGLMQIMPAAARDHEGTIGVSGTARNLSRPEV
NLAFGQRHLQMLRDSGATGGLLPKIMAAYNAGLTPITRWNSEVRDQGDALLYMESIPYWETRGYVAIVMRNYWMYERQAD
ADSSSRKALAEGKWPTFPEVSGATGVRMASAN
>Mature_592_residues
MVPIRVFRSLVVAGVLAGAAFATAMPAPAFAKGAAEYFRARAENNNVPTLLSDSEKAYYAQLFAAIDDERWDTVETLIGQ
RSEGPLHQVALAEYYLHANSPRVELPQIQTWLGRGTRLPMAEQIANLGLKRGLMSAPYLPQEQSFTRQPYITKRIAPRSI
DDGTMPGSIRSAILDRIKNDDPDGARQLLDGIDASLSSAARAEWRKRVAWSYYIENQDAAALAMAKTVSLDGSGAWVAEG
EWVAGLAAWRLGNCGEAADGFRRAAAWAMNPELGAASHYWASRALVRCREPEKAAEQLRGAAMMHETLYGMLAREQLGQT
LPDDGETPDLSPADWQRLRDNGNVRAAVALAEIGRDDLSDEILRHEARIGDPSEYDALSRLARELGLPGTQLWMAQNTPR
GGTYEPALRFPAPRWQPVDGWKVDPALAYAHTLQESNFRPRVVSPAGARGLMQIMPAAARDHEGTIGVSGTARNLSRPEV
NLAFGQRHLQMLRDSGATGGLLPKIMAAYNAGLTPITRWNSEVRDQGDALLYMESIPYWETRGYVAIVMRNYWMYERQAD
ADSSSRKALAEGKWPTFPEVSGATGVRMASAN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=132, Percent_Identity=36.3636363636364, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 65007; Mature: 65007

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPIRVFRSLVVAGVLAGAAFATAMPAPAFAKGAAEYFRARAENNNVPTLLSDSEKAYYA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHH
QLFAAIDDERWDTVETLIGQRSEGPLHQVALAEYYLHANSPRVELPQIQTWLGRGTRLPM
HHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCH
AEQIANLGLKRGLMSAPYLPQEQSFTRQPYITKRIAPRSIDDGTMPGSIRSAILDRIKND
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
DPDGARQLLDGIDASLSSAARAEWRKRVAWSYYIENQDAAALAMAKTVSLDGSGAWVAEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHEEECCCCCEEECC
EWVAGLAAWRLGNCGEAADGFRRAAAWAMNPELGAASHYWASRALVRCREPEKAAEQLRG
CHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
AAMMHETLYGMLAREQLGQTLPDDGETPDLSPADWQRLRDNGNVRAAVALAEIGRDDLSD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHCCCCHHH
EILRHEARIGDPSEYDALSRLARELGLPGTQLWMAQNTPRGGTYEPALRFPAPRWQPVDG
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
WKVDPALAYAHTLQESNFRPRVVSPAGARGLMQIMPAAARDHEGTIGVSGTARNLSRPEV
CCCCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCE
NLAFGQRHLQMLRDSGATGGLLPKIMAAYNAGLTPITRWNSEVRDQGDALLYMESIPYWE
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHCCHHHHCCCCEEEEEECCCCCC
TRGYVAIVMRNYWMYERQADADSSSRKALAEGKWPTFPEVSGATGVRMASAN
CCCEEEEEEEHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MVPIRVFRSLVVAGVLAGAAFATAMPAPAFAKGAAEYFRARAENNNVPTLLSDSEKAYYA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHH
QLFAAIDDERWDTVETLIGQRSEGPLHQVALAEYYLHANSPRVELPQIQTWLGRGTRLPM
HHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCH
AEQIANLGLKRGLMSAPYLPQEQSFTRQPYITKRIAPRSIDDGTMPGSIRSAILDRIKND
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
DPDGARQLLDGIDASLSSAARAEWRKRVAWSYYIENQDAAALAMAKTVSLDGSGAWVAEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHEEECCCCCEEECC
EWVAGLAAWRLGNCGEAADGFRRAAAWAMNPELGAASHYWASRALVRCREPEKAAEQLRG
CHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
AAMMHETLYGMLAREQLGQTLPDDGETPDLSPADWQRLRDNGNVRAAVALAEIGRDDLSD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHCCCCHHH
EILRHEARIGDPSEYDALSRLARELGLPGTQLWMAQNTPRGGTYEPALRFPAPRWQPVDG
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
WKVDPALAYAHTLQESNFRPRVVSPAGARGLMQIMPAAARDHEGTIGVSGTARNLSRPEV
CCCCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCE
NLAFGQRHLQMLRDSGATGGLLPKIMAAYNAGLTPITRWNSEVRDQGDALLYMESIPYWE
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHCCHHHHCCCCEEEEEECCCCCC
TRGYVAIVMRNYWMYERQADADSSSRKALAEGKWPTFPEVSGATGVRMASAN
CCCEEEEEEEHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]