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The map label for this gene is trpE [H]

Identifier: 85374124

GI number: 85374124

Start: 1345696

End: 1347141

Strand: Direct

Name: trpE [H]

Synonym: ELI_06485

Alternate gene names: 85374124

Gene position: 1345696-1347141 (Clockwise)

Preceding gene: 85374123

Following gene: 85374125

Centisome position: 44.09

GC content: 64.8

Gene sequence:

>1446_bases
GTGTGGCGCGAAGTGGTTGCCGATACCGAAACGCCGGTTGGTGCCGCCTTGAAGCTGATCGAACCCGAGCGGGGGGATTT
CCTGCTGGAATCGGTCGAGGGCGGCGAAGTACGCGGACGCTACAGCTTGCTCGGGCTCGACCCGGACCTCGTGTTCCGCG
CCCGCGCCGAGGAGGGCGAAATCAACCGCCTGTGGCAACATGATCGGGCTGCCTTTATCCCCGTCGATGGGAGGAGCCTC
GACGCCCTGCGAGCGCTGATCGAAGAGTGTCGCATCGACGTGCCGGACGGTCTTCCCCCCGCCCTCGCCTGCCTCGTCGG
CTATTTCGCCTATGAAACGATCGGCCAGGTCGAGGTCCTGCCCCGCGCGCCGGAGAGCGAACTCGATTTGCCGGACATGG
TGTTCACCCGGCCGACGCTGCTGCTCGTGTTCGATTCTCTCACCGACAATCTCTTCATCATCGCGCCGTTGTGGCCATCG
AGTGGTGATCCGCAAGCGGCGATCGAGCGCGCGGGCGAGAGAATCGACCACACACTGCGCTGTCTCGCGTCCCCCGATGT
CAGCGAAGTGGCCGCGGACGATTTTCCCGAACTGAATCTGACTCCGCGAATGGCCGAGGACCAGTATTCCTCGATGGTGG
CGAAGGCGAAAGAGTATATCACCGCAGGCGACATCTTTCAGGTCGTGCTGGCGCAGCGGTTCACCTGTCCCTTTCCGCTA
CCACCGCTGGCGCTCTATCGTGCGCTGCGCCGCGTCAATCCCTCGCCGTTTCTCTATTTTCTCGACCTGCCCGGTTTTGC
GGTCGTTGGCTCGAGCCCGGAAATTCTCGTGCGTCTGCGCGATGGCGAGGTCACGATCCGGCCCATCGCCGGGACCCGCC
CGCGCGGCGCGACGCCGGAAGCCGATCGCGAGGCGGAGGCGAGCCTGCTTGCCGATGCCAAGGAGCGTGCCGAGCACCTG
ATGCTGCTCGATCTGGGGCGCAATGATGTCGGGCGGGTCGCCGCCAAGGGCACAGTCGAAGTGACCGACAGTTTCACCGT
CGAGCGCTACAGCCATGTGATGCACATCGTCAGCAATGTTGTCGGACAACTCGATCCGGCCAAGGACGCGCTCGATGCGC
TGTTTGCGGGCTTTCCCGCAGGCACCGTCAGCGGCGCGCCCAAGATCCGTGCCTGCGAGATCATTGCCGAACTGGAGCCC
GAAACGCGCGGTCCCTATGCGGGCGGCGTGGGCTATTTCGCGCCCGACGGCAGCTTCGACAGCTGCATCGTGCTGCGCAC
TGCAGTCCTCAAAGACGGTATGATGCATGTGCAGGCGGGCGCTGGTATCGTCGCCGACAGCGATCCCGCCTATGAACAGC
GTGAATGCGAGGCCAAGGCGGGCGCCCTGATCGCTGCCGCCCGCGAAGCCGCCCGTGTCGCCAGCGAACCCGGATTTGGA
CAGTGA

Upstream 100 bases:

>100_bases
ACCGGCGAAGCCCAGCAATAGGAGGCGGCAACTGTCTGGCGCGGGCCTCCATAATCGCGATGCCGCGCTGGGGCAGCTCG
CTGGCGGTGCGCCCGCGCTG

Downstream 100 bases:

>100_bases
ATTACAGAATACCCTATGTCGCCCTCGCCCTGGCCCTCGCCGCCTGCGGACAGCAAGTCGAAGGCGATCCGGTAACCGTG
ATCGAATTTGGCGAGGATGG

Product: anthranilate/para-aminobenzoate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MWREVVADTETPVGAALKLIEPERGDFLLESVEGGEVRGRYSLLGLDPDLVFRARAEEGEINRLWQHDRAAFIPVDGRSL
DALRALIEECRIDVPDGLPPALACLVGYFAYETIGQVEVLPRAPESELDLPDMVFTRPTLLLVFDSLTDNLFIIAPLWPS
SGDPQAAIERAGERIDHTLRCLASPDVSEVAADDFPELNLTPRMAEDQYSSMVAKAKEYITAGDIFQVVLAQRFTCPFPL
PPLALYRALRRVNPSPFLYFLDLPGFAVVGSSPEILVRLRDGEVTIRPIAGTRPRGATPEADREAEASLLADAKERAEHL
MLLDLGRNDVGRVAAKGTVEVTDSFTVERYSHVMHIVSNVVGQLDPAKDALDALFAGFPAGTVSGAPKIRACEIIAELEP
ETRGPYAGGVGYFAPDGSFDSCIVLRTAVLKDGMMHVQAGAGIVADSDPAYEQRECEAKAGALIAAAREAARVASEPGFG
Q

Sequences:

>Translated_481_residues
MWREVVADTETPVGAALKLIEPERGDFLLESVEGGEVRGRYSLLGLDPDLVFRARAEEGEINRLWQHDRAAFIPVDGRSL
DALRALIEECRIDVPDGLPPALACLVGYFAYETIGQVEVLPRAPESELDLPDMVFTRPTLLLVFDSLTDNLFIIAPLWPS
SGDPQAAIERAGERIDHTLRCLASPDVSEVAADDFPELNLTPRMAEDQYSSMVAKAKEYITAGDIFQVVLAQRFTCPFPL
PPLALYRALRRVNPSPFLYFLDLPGFAVVGSSPEILVRLRDGEVTIRPIAGTRPRGATPEADREAEASLLADAKERAEHL
MLLDLGRNDVGRVAAKGTVEVTDSFTVERYSHVMHIVSNVVGQLDPAKDALDALFAGFPAGTVSGAPKIRACEIIAELEP
ETRGPYAGGVGYFAPDGSFDSCIVLRTAVLKDGMMHVQAGAGIVADSDPAYEQRECEAKAGALIAAAREAARVASEPGFG
Q
>Mature_481_residues
MWREVVADTETPVGAALKLIEPERGDFLLESVEGGEVRGRYSLLGLDPDLVFRARAEEGEINRLWQHDRAAFIPVDGRSL
DALRALIEECRIDVPDGLPPALACLVGYFAYETIGQVEVLPRAPESELDLPDMVFTRPTLLLVFDSLTDNLFIIAPLWPS
SGDPQAAIERAGERIDHTLRCLASPDVSEVAADDFPELNLTPRMAEDQYSSMVAKAKEYITAGDIFQVVLAQRFTCPFPL
PPLALYRALRRVNPSPFLYFLDLPGFAVVGSSPEILVRLRDGEVTIRPIAGTRPRGATPEADREAEASLLADAKERAEHL
MLLDLGRNDVGRVAAKGTVEVTDSFTVERYSHVMHIVSNVVGQLDPAKDALDALFAGFPAGTVSGAPKIRACEIIAELEP
ETRGPYAGGVGYFAPDGSFDSCIVLRTAVLKDGMMHVQAGAGIVADSDPAYEQRECEAKAGALIAAAREAARVASEPGFG
Q

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=369, Percent_Identity=39.0243902439024, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1788114, Length=370, Percent_Identity=34.5945945945946, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87082077, Length=258, Percent_Identity=30.2325581395349, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786809, Length=274, Percent_Identity=28.8321167883212, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320935, Length=468, Percent_Identity=37.6068376068376, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324361, Length=383, Percent_Identity=25.065274151436, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 52066; Mature: 52066

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWREVVADTETPVGAALKLIEPERGDFLLESVEGGEVRGRYSLLGLDPDLVFRARAEEGE
CCCCHHCCCCCCHHHEEEEECCCCCCEEEECCCCCCCCCEEEEEECCHHHEEEECCCCCC
INRLWQHDRAAFIPVDGRSLDALRALIEECRIDVPDGLPPALACLVGYFAYETIGQVEVL
HHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEC
PRAPESELDLPDMVFTRPTLLLVFDSLTDNLFIIAPLWPSSGDPQAAIERAGERIDHTLR
CCCCCCCCCCCCHHHHCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
CLASPDVSEVAADDFPELNLTPRMAEDQYSSMVAKAKEYITAGDIFQVVLAQRFTCPFPL
HHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPLALYRALRRVNPSPFLYFLDLPGFAVVGSSPEILVRLRDGEVTIRPIAGTRPRGATPE
CHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEEEEECCCEEEEECCCCCCCCCCCC
ADREAEASLLADAKERAEHLMLLDLGRNDVGRVAAKGTVEVTDSFTVERYSHVMHIVSNV
CCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCEECCCEEEECCCHHHHHHHHHHHHHHHH
VGQLDPAKDALDALFAGFPAGTVSGAPKIRACEIIAELEPETRGPYAGGVGYFAPDGSFD
HHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SCIVLRTAVLKDGMMHVQAGAGIVADSDPAYEQRECEAKAGALIAAAREAARVASEPGFG
HHHHHHHHHHHCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
Q
C
>Mature Secondary Structure
MWREVVADTETPVGAALKLIEPERGDFLLESVEGGEVRGRYSLLGLDPDLVFRARAEEGE
CCCCHHCCCCCCHHHEEEEECCCCCCEEEECCCCCCCCCEEEEEECCHHHEEEECCCCCC
INRLWQHDRAAFIPVDGRSLDALRALIEECRIDVPDGLPPALACLVGYFAYETIGQVEVL
HHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEC
PRAPESELDLPDMVFTRPTLLLVFDSLTDNLFIIAPLWPSSGDPQAAIERAGERIDHTLR
CCCCCCCCCCCCHHHHCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
CLASPDVSEVAADDFPELNLTPRMAEDQYSSMVAKAKEYITAGDIFQVVLAQRFTCPFPL
HHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPLALYRALRRVNPSPFLYFLDLPGFAVVGSSPEILVRLRDGEVTIRPIAGTRPRGATPE
CHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEEEEECCCEEEEECCCCCCCCCCCC
ADREAEASLLADAKERAEHLMLLDLGRNDVGRVAAKGTVEVTDSFTVERYSHVMHIVSNV
CCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCEECCCEEEECCCHHHHHHHHHHHHHHHH
VGQLDPAKDALDALFAGFPAGTVSGAPKIRACEIIAELEPETRGPYAGGVGYFAPDGSFD
HHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SCIVLRTAVLKDGMMHVQAGAGIVADSDPAYEQRECEAKAGALIAAAREAARVASEPGFG
HHHHHHHHHHHCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA