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The map label for this gene is lpd [H]

Identifier: 85374056

GI number: 85374056

Start: 1272817

End: 1274229

Strand: Direct

Name: lpd [H]

Synonym: ELI_06145

Alternate gene names: 85374056

Gene position: 1272817-1274229 (Clockwise)

Preceding gene: 85374055

Following gene: 85374057

Centisome position: 41.7

GC content: 61.29

Gene sequence:

>1413_bases
ATGGCTGAGAATTACGACGTTATCGTGCTTGGCAGCGGCCCGGGCGGCTATGTCGCGGCGATCCGCTGCGCGCAGCTTGG
TTTGAAGACCGCAATCGTTGAGCGCGAGCTGCTGGGCGGTATATGCCTCAACTGGGGCTGCATCCCGACCAAGGCGCTGC
TACGCTCGGCCGAAATCAAGCACTACATGGAACACGCGAAGGATTACGGGCTCAAGGTCGCCGGTGAAATCGAGGCCGAC
CTCGAAGCCGTCGTGAAGCGCAGCCGCGGCGTCGCCAAGCAGCTCAATCAGGGCGTCACGCACCTGATGAAGAAGAACAA
GATCGCCGTGCATATGGGCGAGGGCACGCTGACCGGCCCGACATCGCTGACGGTGAAAGGCGAGAAGGGAGAGGAGAAAC
TCTCCGCCAAGCATGTGATCGTGGCCACCGGTGCCCGTGCACGCGACCTTCCCTTTGCGCCCGCCGATGGGAAACGCGTG
TGGACCTACCGCACCGCCATGACTCCGCCCGAAATGCCCAAGAAACTGCTCGTCATCGGCTCTGGCGCAATCGGCATCGA
GTTCGCGAGCTTTTACAACGATATGGGCGCGGAAGTTACCGTTGTCGAAATGCTCGACCGGATCGTGCCGGTGGAGGATC
ACGAGGTCTCCGCGTTCCTCGAGAAGTCACTAGGCAAGCAGGGCATGACCATCCTCACCGGCGCGGGGGTCGAGGATCTG
AAGGTGAGCGCCAAGGGCGTGAAGGCCAAGATCAAGACCAAGGACGGCAAGACGGGTGAGCATGACTTCACTCATGCTAT
CGTCGCTATCGGTATTCAGCCGAACACCGAAAACATCGGCCTCGAGAAGCTCGCCGATATGGATCGCGGCTTCATCCAGA
TCGATCCCTACGGGCGAACCAAGTCCAAGGGCCTGTGGGCCATTGGCGACTGTACGCCGGGTCCGTGGCTGGCCCACAAG
GCGAGCCACGAAGGCGTAACTGCCGCAGAGGCTATCGCGCAGGAGCTCGGCAAAGATGGCCTCCACCCGCACCCGCTCAA
TCGCGATGCGATTCCGGGCTGCACCTATTGCCATCCGCAAATCGCCAGCGTTGGGCTGACGGAGGCGAAGGCCAAGGAAG
CAGGCTACGAAGTGAAGGTCGGCAATTTCCCCTTCATCGGAAACGGCAAGGCGATCGCATTGGGCGAGGCCGAGGGCTTC
ACCAAGACCGTCTTCGACGCCGAGACGGGCGAATTGCTCGGCGCGCATATGGTGGGTGCAGAAGTAACCGAGATGATCCA
GGGTTACACCATCGGCAAGACGCTGGAGACGACCGAAACCGAGCTGATGCAGACCGTGTTCCCGCACCCCACCATTTCGG
AATCGATGCACGAAAGCGTCCTTGGCGCCTATGGGCGCGCCTTGCACATCTGA

Upstream 100 bases:

>100_bases
GCCGCGCCCCTATGATGGGCCGTGCCGCATGGGCTGGGTGCTGGCGAAGGATGTGGACTTTCTTGCCGGGTGAACGGCGA
CACACGCGTTTGGAGTGAGA

Downstream 100 bases:

>100_bases
TCGCACCGCAGTGGAGATATCGCCATGTTGAAACGCACCTTTCTCACCATGGCGGCGCTCGCATCGTCTTCGGCAGCGCT
GGCGGAGCCGACCTATGTTG

Product: pyruvate dehydrogenase complex, E3 component, lipoamidedehydrogenase, putative

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAENYDVIVLGSGPGGYVAAIRCAQLGLKTAIVERELLGGICLNWGCIPTKALLRSAEIKHYMEHAKDYGLKVAGEIEAD
LEAVVKRSRGVAKQLNQGVTHLMKKNKIAVHMGEGTLTGPTSLTVKGEKGEEKLSAKHVIVATGARARDLPFAPADGKRV
WTYRTAMTPPEMPKKLLVIGSGAIGIEFASFYNDMGAEVTVVEMLDRIVPVEDHEVSAFLEKSLGKQGMTILTGAGVEDL
KVSAKGVKAKIKTKDGKTGEHDFTHAIVAIGIQPNTENIGLEKLADMDRGFIQIDPYGRTKSKGLWAIGDCTPGPWLAHK
ASHEGVTAAEAIAQELGKDGLHPHPLNRDAIPGCTYCHPQIASVGLTEAKAKEAGYEVKVGNFPFIGNGKAIALGEAEGF
TKTVFDAETGELLGAHMVGAEVTEMIQGYTIGKTLETTETELMQTVFPHPTISESMHESVLGAYGRALHI

Sequences:

>Translated_470_residues
MAENYDVIVLGSGPGGYVAAIRCAQLGLKTAIVERELLGGICLNWGCIPTKALLRSAEIKHYMEHAKDYGLKVAGEIEAD
LEAVVKRSRGVAKQLNQGVTHLMKKNKIAVHMGEGTLTGPTSLTVKGEKGEEKLSAKHVIVATGARARDLPFAPADGKRV
WTYRTAMTPPEMPKKLLVIGSGAIGIEFASFYNDMGAEVTVVEMLDRIVPVEDHEVSAFLEKSLGKQGMTILTGAGVEDL
KVSAKGVKAKIKTKDGKTGEHDFTHAIVAIGIQPNTENIGLEKLADMDRGFIQIDPYGRTKSKGLWAIGDCTPGPWLAHK
ASHEGVTAAEAIAQELGKDGLHPHPLNRDAIPGCTYCHPQIASVGLTEAKAKEAGYEVKVGNFPFIGNGKAIALGEAEGF
TKTVFDAETGELLGAHMVGAEVTEMIQGYTIGKTLETTETELMQTVFPHPTISESMHESVLGAYGRALHI
>Mature_469_residues
AENYDVIVLGSGPGGYVAAIRCAQLGLKTAIVERELLGGICLNWGCIPTKALLRSAEIKHYMEHAKDYGLKVAGEIEADL
EAVVKRSRGVAKQLNQGVTHLMKKNKIAVHMGEGTLTGPTSLTVKGEKGEEKLSAKHVIVATGARARDLPFAPADGKRVW
TYRTAMTPPEMPKKLLVIGSGAIGIEFASFYNDMGAEVTVVEMLDRIVPVEDHEVSAFLEKSLGKQGMTILTGAGVEDLK
VSAKGVKAKIKTKDGKTGEHDFTHAIVAIGIQPNTENIGLEKLADMDRGFIQIDPYGRTKSKGLWAIGDCTPGPWLAHKA
SHEGVTAAEAIAQELGKDGLHPHPLNRDAIPGCTYCHPQIASVGLTEAKAKEAGYEVKVGNFPFIGNGKAIALGEAEGFT
KTVFDAETGELLGAHMVGAEVTEMIQGYTIGKTLETTETELMQTVFPHPTISESMHESVLGAYGRALHI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=485, Percent_Identity=36.0824742268041, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=27.6231263383298, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI22035672, Length=491, Percent_Identity=28.3095723014257, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33519430, Length=463, Percent_Identity=23.7580993520518, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI33519428, Length=463, Percent_Identity=23.7580993520518, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI33519426, Length=463, Percent_Identity=23.7580993520518, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI148277065, Length=463, Percent_Identity=23.3261339092873, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI148277071, Length=440, Percent_Identity=23.8636363636364, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045266, Length=433, Percent_Identity=24.9422632794457, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI291045268, Length=282, Percent_Identity=26.241134751773, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786307, Length=453, Percent_Identity=36.4238410596026, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI87082354, Length=485, Percent_Identity=27.8350515463918, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=25.5965292841649, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789915, Length=467, Percent_Identity=27.1948608137045, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=483, Percent_Identity=35.1966873706004, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=24.8434237995825, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=462, Percent_Identity=24.6753246753247, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=25.0535331905782, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=24.4306418219462, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=249, Percent_Identity=26.1044176706827, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=492, Percent_Identity=36.9918699186992, Blast_Score=273, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=27.4633123689727, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=477, Percent_Identity=26.4150943396226, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=36.1344537815126, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=25.4697286012526, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=25.4697286012526, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24640551, Length=479, Percent_Identity=25.4697286012526, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=24.847250509165, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50195; Mature: 50064

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENYDVIVLGSGPGGYVAAIRCAQLGLKTAIVERELLGGICLNWGCIPTKALLRSAEIK
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHH
HYMEHAKDYGLKVAGEIEADLEAVVKRSRGVAKQLNQGVTHLMKKNKIAVHMGEGTLTGP
HHHHHHHHCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
TSLTVKGEKGEEKLSAKHVIVATGARARDLPFAPADGKRVWTYRTAMTPPEMPKKLLVIG
CEEEEECCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
SGAIGIEFASFYNDMGAEVTVVEMLDRIVPVEDHEVSAFLEKSLGKQGMTILTGAGVEDL
CCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCHHHE
KVSAKGVKAKIKTKDGKTGEHDFTHAIVAIGIQPNTENIGLEKLADMDRGFIQIDPYGRT
EEECCCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCC
KSKGLWAIGDCTPGPWLAHKASHEGVTAAEAIAQELGKDGLHPHPLNRDAIPGCTYCHPQ
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
IASVGLTEAKAKEAGYEVKVGNFPFIGNGKAIALGEAEGFTKTVFDAETGELLGAHMVGA
HHHCCCCHHHHHHCCCEEEECCCCEECCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHH
EVTEMIQGYTIGKTLETTETELMQTVFPHPTISESMHESVLGAYGRALHI
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure 
AENYDVIVLGSGPGGYVAAIRCAQLGLKTAIVERELLGGICLNWGCIPTKALLRSAEIK
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHH
HYMEHAKDYGLKVAGEIEADLEAVVKRSRGVAKQLNQGVTHLMKKNKIAVHMGEGTLTGP
HHHHHHHHCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
TSLTVKGEKGEEKLSAKHVIVATGARARDLPFAPADGKRVWTYRTAMTPPEMPKKLLVIG
CEEEEECCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
SGAIGIEFASFYNDMGAEVTVVEMLDRIVPVEDHEVSAFLEKSLGKQGMTILTGAGVEDL
CCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCHHHE
KVSAKGVKAKIKTKDGKTGEHDFTHAIVAIGIQPNTENIGLEKLADMDRGFIQIDPYGRT
EEECCCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCC
KSKGLWAIGDCTPGPWLAHKASHEGVTAAEAIAQELGKDGLHPHPLNRDAIPGCTYCHPQ
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
IASVGLTEAKAKEAGYEVKVGNFPFIGNGKAIALGEAEGFTKTVFDAETGELLGAHMVGA
HHHCCCCHHHHHHCCCEEEECCCCEECCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHH
EVTEMIQGYTIGKTLETTETELMQTVFPHPTISESMHESVLGAYGRALHI
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]