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The map label for this gene is pdhC [H]

Identifier: 85374053

GI number: 85374053

Start: 1270110

End: 1271423

Strand: Direct

Name: pdhC [H]

Synonym: ELI_06130

Alternate gene names: 85374053

Gene position: 1270110-1271423 (Clockwise)

Preceding gene: 85374051

Following gene: 85374054

Centisome position: 41.61

GC content: 63.32

Gene sequence:

>1314_bases
ATGCCGACACCGATCAAGATGCCCGCGCTTTCGCCGACGATGGAGGAAGGCACGCTCGCCAAATGGCTGGTCAAGGTCGG
CGATACAATCGGTGCGGGCGACATCATGGCCGAGATCGAAACCGACAAGGCCACGATGGAGTTCGAGGCTGTCGACGAAG
GTACTGTAGCCGAAATCCTGATCGACGAAGGCAGCGAGAACGTGAAGGTCGGCGAGGTCATCATGATCCTCGCGGAAGAG
GGCGAGGACATCGAGGAGGCCAAGGCGGCGGCACCGCAAAAGTCCGATGCGACCTCAGAGACCGTTCGGGCTGAGCCAGT
CGAGGCCCTGTCCTCCACTTCCGCCCCGCCTGCAACGAAAAAGGACGACCCTTCGACAGGCTCAGGGCGAACGGAAAGTG
GCGGGTCTGCCAGTTCCTCGGGCGATCGCATCATCGCCTCACCGCTGGCCAAGCGGATTGCCGAGCAGAAAGGTCTCGAT
CTTTCGACCGTCACCGGCAGCGGCCCGAATGGCCGGATCATCAAAGCCGATGTCGAAGGAGCGGAAGCGGGCGAGGCTCC
CGACAAGGCTGATGCTCCGGCTCCAGCACAGGCCAAGCAACCGTCCCTGGGCGGCGATCTCGATGCGCCCTACGAGGCCG
AAAAGCTCAACAACGTCCGCAAGGTCATTGCGCGCCGCCTGACCGAAGCGAAGCAGACCATTCCGCATATCTACTTGACC
GTGGACGTGCGGCTGGATGCGCTGCTGGACCTGCGCAAGCAGTTGAACGCCTCACTCGAAGCCGATGGGGTCAAGCTCTC
AGTCAACGACCTGCTGATCAAAGCGCTCGCTCGCGCGCTCCAGCGCGTGCCCAAGTGCAACGTCAGCTTCCAGGGCGACG
AGCTCTATCAATACACCCGCGAGGACATCTCCGTGGCGGTGGCCGCGCCTTCTGGTCTCATCACGCCGATCATCCGCGAT
GCCGGTCGCAAGGGCCTCGCGCAGATCAGTACCGAAATGAAAGAACTCGCCGGCAAGGCGAAGGACGGCAAGCTGCAGCC
GCAGGAATACCAGGGCGGCACCGCCTCGCTGTCCAATCTCGGCATGTTCGGCACCAAGCAGTTCGATGCAGTGATCAATC
CGCCACAGGCCATGATCCTCGCCGTGGGCGCAGGCGAGCAGCGCCCGCATGTCATCGACGGCGCACTCGGCGTCGCCATG
GTGATGAGCGCCACCGGCAGCTTCGACCACCGCGCCATCGACGGCGCAGACGGCGCGCAACTGATGGAGGCGTTCCAGCA
GCTGTGCGAGAACCCGATGGGGCTGGTGGTTTAA

Upstream 100 bases:

>100_bases
TTGTCCGAAGAACCTTGCCGTGCCCGCCCAAATTGGCCAAAGCGTAGCGGAATTCGCGCTTCAGAGCGCGCAGCCCAGCG
AAGGACCTGCAGACCCGCCT

Downstream 100 bases:

>100_bases
CTAGTGGACCTCGTCCCCTTAATTGCCGCAGGAATTGGGTTTTCCGGCTTGCTGTTAGGCCTGATCCTCAGACCTTGGGT
AGAGGAATGGTTCGCTCGCC

Product: pyruvate dehydrogenase E2 component

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPTPIKMPALSPTMEEGTLAKWLVKVGDTIGAGDIMAEIETDKATMEFEAVDEGTVAEILIDEGSENVKVGEVIMILAEE
GEDIEEAKAAAPQKSDATSETVRAEPVEALSSTSAPPATKKDDPSTGSGRTESGGSASSSGDRIIASPLAKRIAEQKGLD
LSTVTGSGPNGRIIKADVEGAEAGEAPDKADAPAPAQAKQPSLGGDLDAPYEAEKLNNVRKVIARRLTEAKQTIPHIYLT
VDVRLDALLDLRKQLNASLEADGVKLSVNDLLIKALARALQRVPKCNVSFQGDELYQYTREDISVAVAAPSGLITPIIRD
AGRKGLAQISTEMKELAGKAKDGKLQPQEYQGGTASLSNLGMFGTKQFDAVINPPQAMILAVGAGEQRPHVIDGALGVAM
VMSATGSFDHRAIDGADGAQLMEAFQQLCENPMGLVV

Sequences:

>Translated_437_residues
MPTPIKMPALSPTMEEGTLAKWLVKVGDTIGAGDIMAEIETDKATMEFEAVDEGTVAEILIDEGSENVKVGEVIMILAEE
GEDIEEAKAAAPQKSDATSETVRAEPVEALSSTSAPPATKKDDPSTGSGRTESGGSASSSGDRIIASPLAKRIAEQKGLD
LSTVTGSGPNGRIIKADVEGAEAGEAPDKADAPAPAQAKQPSLGGDLDAPYEAEKLNNVRKVIARRLTEAKQTIPHIYLT
VDVRLDALLDLRKQLNASLEADGVKLSVNDLLIKALARALQRVPKCNVSFQGDELYQYTREDISVAVAAPSGLITPIIRD
AGRKGLAQISTEMKELAGKAKDGKLQPQEYQGGTASLSNLGMFGTKQFDAVINPPQAMILAVGAGEQRPHVIDGALGVAM
VMSATGSFDHRAIDGADGAQLMEAFQQLCENPMGLVV
>Mature_436_residues
PTPIKMPALSPTMEEGTLAKWLVKVGDTIGAGDIMAEIETDKATMEFEAVDEGTVAEILIDEGSENVKVGEVIMILAEEG
EDIEEAKAAAPQKSDATSETVRAEPVEALSSTSAPPATKKDDPSTGSGRTESGGSASSSGDRIIASPLAKRIAEQKGLDL
STVTGSGPNGRIIKADVEGAEAGEAPDKADAPAPAQAKQPSLGGDLDAPYEAEKLNNVRKVIARRLTEAKQTIPHIYLTV
DVRLDALLDLRKQLNASLEADGVKLSVNDLLIKALARALQRVPKCNVSFQGDELYQYTREDISVAVAAPSGLITPIIRDA
GRKGLAQISTEMKELAGKAKDGKLQPQEYQGGTASLSNLGMFGTKQFDAVINPPQAMILAVGAGEQRPHVIDGALGVAMV
MSATGSFDHRAIDGADGAQLMEAFQQLCENPMGLVV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=453, Percent_Identity=41.9426048565121, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI203098753, Length=474, Percent_Identity=38.3966244725738, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI203098816, Length=474, Percent_Identity=38.3966244725738, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=45.5089820359281, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI110671329, Length=452, Percent_Identity=24.3362831858407, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI19923748, Length=241, Percent_Identity=32.3651452282158, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786946, Length=437, Percent_Identity=29.7482837528604, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=30.5361305361305, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=43.8202247191011, Blast_Score=311, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI17538894, Length=304, Percent_Identity=37.828947368421, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=436, Percent_Identity=29.1284403669725, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=436, Percent_Identity=26.8348623853211, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=38.8646288209607, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6320352, Length=442, Percent_Identity=28.0542986425339, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321632, Length=178, Percent_Identity=35.3932584269663, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20129315, Length=449, Percent_Identity=39.1982182628062, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24582497, Length=434, Percent_Identity=38.9400921658986, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=28.538283062645, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=33.6322869955157, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45805; Mature: 45674

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPIKMPALSPTMEEGTLAKWLVKVGDTIGAGDIMAEIETDKATMEFEAVDEGTVAEIL
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IDEGSENVKVGEVIMILAEEGEDIEEAKAAAPQKSDATSETVRAEPVEALSSTSAPPATK
EECCCCCEEECEEEEEEECCCCCHHHHHHCCCCCCCCCHHHHHHCHHHHHHCCCCCCCCC
KDDPSTGSGRTESGGSASSSGDRIIASPLAKRIAEQKGLDLSTVTGSGPNGRIIKADVEG
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCC
AEAGEAPDKADAPAPAQAKQPSLGGDLDAPYEAEKLNNVRKVIARRLTEAKQTIPHIYLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VDVRLDALLDLRKQLNASLEADGVKLSVNDLLIKALARALQRVPKCNVSFQGDELYQYTR
EEEHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHH
EDISVAVAAPSGLITPIIRDAGRKGLAQISTEMKELAGKAKDGKLQPQEYQGGTASLSNL
HCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHC
GMFGTKQFDAVINPPQAMILAVGAGEQRPHVIDGALGVAMVMSATGSFDHRAIDGADGAQ
CCCCCCCCHHHCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LMEAFQQLCENPMGLVV
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PTPIKMPALSPTMEEGTLAKWLVKVGDTIGAGDIMAEIETDKATMEFEAVDEGTVAEIL
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IDEGSENVKVGEVIMILAEEGEDIEEAKAAAPQKSDATSETVRAEPVEALSSTSAPPATK
EECCCCCEEECEEEEEEECCCCCHHHHHHCCCCCCCCCHHHHHHCHHHHHHCCCCCCCCC
KDDPSTGSGRTESGGSASSSGDRIIASPLAKRIAEQKGLDLSTVTGSGPNGRIIKADVEG
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCC
AEAGEAPDKADAPAPAQAKQPSLGGDLDAPYEAEKLNNVRKVIARRLTEAKQTIPHIYLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VDVRLDALLDLRKQLNASLEADGVKLSVNDLLIKALARALQRVPKCNVSFQGDELYQYTR
EEEHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHH
EDISVAVAAPSGLITPIIRDAGRKGLAQISTEMKELAGKAKDGKLQPQEYQGGTASLSNL
HCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHC
GMFGTKQFDAVINPPQAMILAVGAGEQRPHVIDGALGVAMVMSATGSFDHRAIDGADGAQ
CCCCCCCCHHHCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LMEAFQQLCENPMGLVV
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]