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The map label for this gene is pntB [H]

Identifier: 85373297

GI number: 85373297

Start: 509536

End: 510939

Strand: Direct

Name: pntB [H]

Synonym: ELI_02350

Alternate gene names: 85373297

Gene position: 509536-510939 (Clockwise)

Preceding gene: 85373296

Following gene: 85373298

Centisome position: 16.69

GC content: 63.82

Gene sequence:

>1404_bases
GTGAACCCCTGGGTCGCGCTGGCATACCTCGTTGCGGGCGTGTTCTTCATTCTCGCGCTGCGCGGGCTGTCCAGCCCTGC
GACGAGCCGCGCGGGCAATCGTTTCGGCATGGTCGGGATGCTGCTGGCGGTGGTGACGACGCTCGTCACACACGACATCG
CCAATATCGTCGAGATCGGCATTGCGATCCTGATTGGCGGGATCGTCGGCTTCACCATCGCTCGTCGCATCGCCATGACC
GCGATGCCGGAACTGGTCGCGGCCTTCCACTCGCTGGTCGGCATGGCGGCGGTACTGGTCGGCTGGGCGGCCTATCTCAA
CCCCGGCGCCTTCGGGTTGCTGGTGGGTGACAGCATAGGCGCGGTCAGCAAGATCGAGATGGGGCTGGGCATCGGCATCG
GCGCGATCACCTTCTCGGGTTCGGTCATCGCTTTCGCCAAGCTATCCGGCAAGATGAGCGGCGCGCCGATCCTGCTGCCT
GCGCGGCATGTCATCAACCTTGGCGTCCTTGCGGCCATCATCATACTGACGGGGCTCTTCGCGATGGCGCAGGGTCCGGC
GGAGACGCTGCCCTATATCGTCGCCATCACCGTGCTGGCTTTCGCCATCGGCTTCCTGCTGATCATCCCGATCGGCGGGG
CGGACATGCCGGTCGTGGTGTCGATGCTGAACAGCTATTCGGGCTGGGCCGCGGCGGCGATGGGCTTCACGCTCGGCAAT
ACGGCGATGATCATCACCGGGGCGCTGGTTGGCTCTTCGGGCGCGATCCTCAGCTACATCATGTGCCGCGCTATGAACCG
CAGCTTCATCAGCGTCATCGCGGGCGGCTTCGGTGCGGACGACAGCGCGGGTGGCAGCGAAGCGCGCGAGCAGCGGCCTT
ACAAGCAGGGCAGCGCGGACGACGCCGCTTTCATGCTCGAACAGGCCGAGAAGGTCATCATCATCCCCGGCTACGGCATG
GCGGTGGCGCAGGCGCAGCACGCCCTGCGCGAAATGGCCGACCTGCTGGAGGCCAAGGGCGTCGAGGTCAAATACGCCAT
CCACCCCGTCGCGGGCCGCATGCCCGGCCACATGAACGTGCTTTTGGCCGAAGCCAGCGTGCCCTACGAAAACGTGTTCG
AATTGGAGGACATCAACTCGGAATTCGCGCAGGCCGATGTAGCCTTTATCATCGGCGCGAACGACGTGGTGAACCCGGCG
GCCAAAACCGACAAGAGCTCGCCGATCTACGGCATGCCCGTGTTCGACGTCGACAAGGCCAAGCAGGTCTTTTTCATCAA
GCGCAGCATGGGCGGGGTCGGCTATGCCGGGGTCGATAACGACGTCTTCTACATGGACCAGACCATGATGTTGCTGTCCG
ACGCCAAAAAGATGGTCGAGGAAATCAACAAGGCGCTCGACTAG

Upstream 100 bases:

>100_bases
AACGCTCAAGGCAGCGGGACCCCGGATCAAGTCCGGGGTGACGGCTCTTCTTTTGACTGGTTTTGGCGCCACCCCCGCCC
ACGCCGCAACCGGCGAGGCG

Downstream 100 bases:

>100_bases
TGCGCAGCTTCGCGGCGGCGATTTGCGCCGCGCTGCTCCTCACAGGCTGCGTCGAGAAAATCGCCGAGAACCGTGTCGAG
AACGCCTTGCTCAAGGCCGG

Product: PntB, NAD(P) transhydrogenase, beta subunit

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MNPWVALAYLVAGVFFILALRGLSSPATSRAGNRFGMVGMLLAVVTTLVTHDIANIVEIGIAILIGGIVGFTIARRIAMT
AMPELVAAFHSLVGMAAVLVGWAAYLNPGAFGLLVGDSIGAVSKIEMGLGIGIGAITFSGSVIAFAKLSGKMSGAPILLP
ARHVINLGVLAAIIILTGLFAMAQGPAETLPYIVAITVLAFAIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTLGN
TAMIITGALVGSSGAILSYIMCRAMNRSFISVIAGGFGADDSAGGSEAREQRPYKQGSADDAAFMLEQAEKVIIIPGYGM
AVAQAQHALREMADLLEAKGVEVKYAIHPVAGRMPGHMNVLLAEASVPYENVFELEDINSEFAQADVAFIIGANDVVNPA
AKTDKSSPIYGMPVFDVDKAKQVFFIKRSMGGVGYAGVDNDVFYMDQTMMLLSDAKKMVEEINKALD

Sequences:

>Translated_467_residues
MNPWVALAYLVAGVFFILALRGLSSPATSRAGNRFGMVGMLLAVVTTLVTHDIANIVEIGIAILIGGIVGFTIARRIAMT
AMPELVAAFHSLVGMAAVLVGWAAYLNPGAFGLLVGDSIGAVSKIEMGLGIGIGAITFSGSVIAFAKLSGKMSGAPILLP
ARHVINLGVLAAIIILTGLFAMAQGPAETLPYIVAITVLAFAIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTLGN
TAMIITGALVGSSGAILSYIMCRAMNRSFISVIAGGFGADDSAGGSEAREQRPYKQGSADDAAFMLEQAEKVIIIPGYGM
AVAQAQHALREMADLLEAKGVEVKYAIHPVAGRMPGHMNVLLAEASVPYENVFELEDINSEFAQADVAFIIGANDVVNPA
AKTDKSSPIYGMPVFDVDKAKQVFFIKRSMGGVGYAGVDNDVFYMDQTMMLLSDAKKMVEEINKALD
>Mature_467_residues
MNPWVALAYLVAGVFFILALRGLSSPATSRAGNRFGMVGMLLAVVTTLVTHDIANIVEIGIAILIGGIVGFTIARRIAMT
AMPELVAAFHSLVGMAAVLVGWAAYLNPGAFGLLVGDSIGAVSKIEMGLGIGIGAITFSGSVIAFAKLSGKMSGAPILLP
ARHVINLGVLAAIIILTGLFAMAQGPAETLPYIVAITVLAFAIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTLGN
TAMIITGALVGSSGAILSYIMCRAMNRSFISVIAGGFGADDSAGGSEAREQRPYKQGSADDAAFMLEQAEKVIIIPGYGM
AVAQAQHALREMADLLEAKGVEVKYAIHPVAGRMPGHMNVLLAEASVPYENVFELEDINSEFAQADVAFIIGANDVVNPA
AKTDKSSPIYGMPVFDVDKAKQVFFIKRSMGGVGYAGVDNDVFYMDQTMMLLSDAKKMVEEINKALD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=459, Percent_Identity=46.4052287581699, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI122939153, Length=459, Percent_Identity=46.4052287581699, Blast_Score=390, Evalue=1e-108,
Organism=Escherichia coli, GI1787886, Length=464, Percent_Identity=52.801724137931, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17550456, Length=459, Percent_Identity=45.0980392156863, Blast_Score=370, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48708; Mature: 48708

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPWVALAYLVAGVFFILALRGLSSPATSRAGNRFGMVGMLLAVVTTLVTHDIANIVEIG
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAILIGGIVGFTIARRIAMTAMPELVAAFHSLVGMAAVLVGWAAYLNPGAFGLLVGDSIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
AVSKIEMGLGIGIGAITFSGSVIAFAKLSGKMSGAPILLPARHVINLGVLAAIIILTGLF
CHHHHHHCCCCCEEHEEECCCEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHH
AMAQGPAETLPYIVAITVLAFAIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTLGN
HHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCC
TAMIITGALVGSSGAILSYIMCRAMNRSFISVIAGGFGADDSAGGSEAREQRPYKQGSAD
CEEEEEEHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCC
DAAFMLEQAEKVIIIPGYGMAVAQAQHALREMADLLEAKGVEVKYAIHPVAGRMPGHMNV
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEE
LLAEASVPYENVFELEDINSEFAQADVAFIIGANDVVNPAAKTDKSSPIYGMPVFDVDKA
EEEECCCCHHHHHHHHHCCHHHHHCCEEEEECCCHHCCCCCCCCCCCCEECCCCCCCCCC
KQVFFIKRSMGGVGYAGVDNDVFYMDQTMMLLSDAKKMVEEINKALD
HHEEEEEECCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPWVALAYLVAGVFFILALRGLSSPATSRAGNRFGMVGMLLAVVTTLVTHDIANIVEIG
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAILIGGIVGFTIARRIAMTAMPELVAAFHSLVGMAAVLVGWAAYLNPGAFGLLVGDSIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
AVSKIEMGLGIGIGAITFSGSVIAFAKLSGKMSGAPILLPARHVINLGVLAAIIILTGLF
CHHHHHHCCCCCEEHEEECCCEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHH
AMAQGPAETLPYIVAITVLAFAIGFLLIIPIGGADMPVVVSMLNSYSGWAAAAMGFTLGN
HHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCC
TAMIITGALVGSSGAILSYIMCRAMNRSFISVIAGGFGADDSAGGSEAREQRPYKQGSAD
CEEEEEEHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCC
DAAFMLEQAEKVIIIPGYGMAVAQAQHALREMADLLEAKGVEVKYAIHPVAGRMPGHMNV
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEE
LLAEASVPYENVFELEDINSEFAQADVAFIIGANDVVNPAAKTDKSSPIYGMPVFDVDKA
EEEECCCCHHHHHHHHHCCHHHHHCCEEEEECCCHHCCCCCCCCCCCCEECCCCCCCCCC
KQVFFIKRSMGGVGYAGVDNDVFYMDQTMMLLSDAKKMVEEINKALD
HHEEEEEECCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]