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The map label for this gene is iscS [H]

Identifier: 85373267

GI number: 85373267

Start: 479667

End: 480743

Strand: Direct

Name: iscS [H]

Synonym: ELI_02200

Alternate gene names: 85373267

Gene position: 479667-480743 (Clockwise)

Preceding gene: 85373266

Following gene: 85373268

Centisome position: 15.71

GC content: 65.65

Gene sequence:

>1077_bases
ATGATCTACCTCGACTATCAGGCCACCAGTCCGCTCGCGCCGGAAGCGCGGGACGAAATGCTGCGCTGGCTCGACGGGCC
GGACGGCACCGGCTTCGGCAATCCGCACTCGCCGCACCGGATGGGGCGACAGGCGGCGGCGGCGATCGAACTGGCGCGCG
ACCGGGTGGCGGCCTTGTTCCCAGCTGGGGGCCGGGTGATCTTCACGGGCGGCGCGACCGAGGCGGTCAATCTCGCTATT
CGCGGCACCGCGCATCTGGGCGGCAGCGTCTCGGTCTCCGCCATCGAGCATGCCGCCGTGCTCGATACCGCGCGCGATCT
GGGGCAGGCGCATGTGCTCAATGTCGCCAGGGACGGGCAGTGCAATACCAAGCAGGACCTGCCCAATGACTGCCGCCTTG
TCTGCCTGATGCAGGTCAACAACGAAATCGGCGTGATCCAACCGACGGTCGACTGGCATCGCAAGGCCAAGGGCAACAAT
GCGCTCTATCTGTGCGACGCGGTGCAGGCCTATGGCAAGATGGAAGTGACGGGCGCAGACATGATCGCCATCAGTGCGCA
CAAGTTTCACGGGCCCAAAGGCGTTGGCGCACTGTGGGTGCGCGACGGGGTCGATTTGCACGAGGTTCAGACCGGCGGCG
GGCAGGAATTCGGCCTGCGTTCCGGCACCTTGTCTCCGGCGCTGATCGCCGGGATGGGTGCGGCGGCGGCAGAGGCGAGC
GACCGCATGGAGCGCGACGCGGATCATGTCGGCAATCTGTGGCATCGGGCTCGCGAGATCTTCGCGGACTGGGATCTCAA
TGGCAGCGCCGAGGCGCGCTATTTCGGTAATCTCAATATTCGCCGGAAGGGCCTGGATGTGAACCGCCTGATGAGCGAAT
GTCGCGACGTCATGTTCAGCGCCGGCAGCGCCTGCGCCAGCGGCTCGGGCCGCACCAGCCATGTGCTGGAAGCGATCGGA
CTGTCCAAGAAACAGGCTAAAAGCTCGATCCGATTGGGCTTCGGCCGCTACACCACGATGGACGAGATCGAGCAGGCCGC
CACCACCATCAACCAGGCGGCGGAAGCGCAGCTGTGA

Upstream 100 bases:

>100_bases
CGCAGCGTCGACGGCTGTACTTTCTTGCTGTTCCGCGTCCGCCAAAAGGAAGAACAGGGCTTCGACAAGCTCAGCCCGAA
CGGATAGAGGGCAAGGCACG

Downstream 100 bases:

>100_bases
AAATCGTGTTCGAAACCGCCGAAGGAGCGGCGGTCGAGGCCGAGGCCGCCCCAGGCGACACCCTGCTCGCGATAGCCCAA
GGCGCGGGCCTGCCGCTCGA

Product: aminotransferase NifS, class V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MIYLDYQATSPLAPEARDEMLRWLDGPDGTGFGNPHSPHRMGRQAAAAIELARDRVAALFPAGGRVIFTGGATEAVNLAI
RGTAHLGGSVSVSAIEHAAVLDTARDLGQAHVLNVARDGQCNTKQDLPNDCRLVCLMQVNNEIGVIQPTVDWHRKAKGNN
ALYLCDAVQAYGKMEVTGADMIAISAHKFHGPKGVGALWVRDGVDLHEVQTGGGQEFGLRSGTLSPALIAGMGAAAAEAS
DRMERDADHVGNLWHRAREIFADWDLNGSAEARYFGNLNIRRKGLDVNRLMSECRDVMFSAGSACASGSGRTSHVLEAIG
LSKKQAKSSIRLGFGRYTTMDEIEQAATTINQAAEAQL

Sequences:

>Translated_358_residues
MIYLDYQATSPLAPEARDEMLRWLDGPDGTGFGNPHSPHRMGRQAAAAIELARDRVAALFPAGGRVIFTGGATEAVNLAI
RGTAHLGGSVSVSAIEHAAVLDTARDLGQAHVLNVARDGQCNTKQDLPNDCRLVCLMQVNNEIGVIQPTVDWHRKAKGNN
ALYLCDAVQAYGKMEVTGADMIAISAHKFHGPKGVGALWVRDGVDLHEVQTGGGQEFGLRSGTLSPALIAGMGAAAAEAS
DRMERDADHVGNLWHRAREIFADWDLNGSAEARYFGNLNIRRKGLDVNRLMSECRDVMFSAGSACASGSGRTSHVLEAIG
LSKKQAKSSIRLGFGRYTTMDEIEQAATTINQAAEAQL
>Mature_358_residues
MIYLDYQATSPLAPEARDEMLRWLDGPDGTGFGNPHSPHRMGRQAAAAIELARDRVAALFPAGGRVIFTGGATEAVNLAI
RGTAHLGGSVSVSAIEHAAVLDTARDLGQAHVLNVARDGQCNTKQDLPNDCRLVCLMQVNNEIGVIQPTVDWHRKAKGNN
ALYLCDAVQAYGKMEVTGADMIAISAHKFHGPKGVGALWVRDGVDLHEVQTGGGQEFGLRSGTLSPALIAGMGAAAAEAS
DRMERDADHVGNLWHRAREIFADWDLNGSAEARYFGNLNIRRKGLDVNRLMSECRDVMFSAGSACASGSGRTSHVLEAIG
LSKKQAKSSIRLGFGRYTTMDEIEQAATTINQAAEAQL

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=366, Percent_Identity=39.344262295082, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=30.3827751196172, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI48994898, Length=375, Percent_Identity=38.1333333333333, Blast_Score=212, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25143064, Length=368, Percent_Identity=38.3152173913043, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=27.82874617737, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319831, Length=373, Percent_Identity=37.8016085790885, Blast_Score=220, Evalue=2e-58,
Organism=Drosophila melanogaster, GI20129463, Length=369, Percent_Identity=39.5663956639566, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 38260; Mature: 38260

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDYQATSPLAPEARDEMLRWLDGPDGTGFGNPHSPHRMGRQAAAAIELARDRVAALF
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
PAGGRVIFTGGATEAVNLAIRGTAHLGGSVSVSAIEHAAVLDTARDLGQAHVLNVARDGQ
CCCCEEEEECCCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCHHHEEEEECCCC
CNTKQDLPNDCRLVCLMQVNNEIGVIQPTVDWHRKAKGNNALYLCDAVQAYGKMEVTGAD
CCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHCCCCCEEEEEEHHHHCCCEEECCCC
MIAISAHKFHGPKGVGALWVRDGVDLHEVQTGGGQEFGLRSGTLSPALIAGMGAAAAEAS
EEEEEEHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHHHH
DRMERDADHVGNLWHRAREIFADWDLNGSAEARYFGNLNIRRKGLDVNRLMSECRDVMFS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHH
AGSACASGSGRTSHVLEAIGLSKKQAKSSIRLGFGRYTTMDEIEQAATTINQAAEAQL
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYLDYQATSPLAPEARDEMLRWLDGPDGTGFGNPHSPHRMGRQAAAAIELARDRVAALF
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
PAGGRVIFTGGATEAVNLAIRGTAHLGGSVSVSAIEHAAVLDTARDLGQAHVLNVARDGQ
CCCCEEEEECCCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCHHHEEEEECCCC
CNTKQDLPNDCRLVCLMQVNNEIGVIQPTVDWHRKAKGNNALYLCDAVQAYGKMEVTGAD
CCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHCCCCCEEEEEEHHHHCCCEEECCCC
MIAISAHKFHGPKGVGALWVRDGVDLHEVQTGGGQEFGLRSGTLSPALIAGMGAAAAEAS
EEEEEEHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHHHH
DRMERDADHVGNLWHRAREIFADWDLNGSAEARYFGNLNIRRKGLDVNRLMSECRDVMFS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHH
AGSACASGSGRTSHVLEAIGLSKKQAKSSIRLGFGRYTTMDEIEQAATTINQAAEAQL
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]