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The map label for this gene is pckA [H]

Identifier: 85373255

GI number: 85373255

Start: 469487

End: 471103

Strand: Direct

Name: pckA [H]

Synonym: ELI_02140

Alternate gene names: 85373255

Gene position: 469487-471103 (Clockwise)

Preceding gene: 85373246

Following gene: 85373256

Centisome position: 15.38

GC content: 62.03

Gene sequence:

>1617_bases
GTGACCGCTTTGTCTACCAGTCTTGCCGATCAGGGCATTTCGACCAATGCCACGATCCATCCCAACCTCGGCACCGCGCA
GCTCGTCGAGCATGCCATCGACAACGGCGAAGGCCGCCTGTCGAAATATGGCAGCCTGGTGGTGGAAACGGGCAAGCACA
CCGGCCGCAGCGCGAAAGACAAGTTCATTGTCCGCGATAGCGAAACCGAAGATACCGTGTGGTGGGACAACAATGCTTCG
ATCGATCCAGAGCATTTCGCCGCGCTGAAGGAAGATTTCCTCAAGGCCGTGGCCGAAAAGGACACGCTCTACGTCGCCGA
CCTGTTCGGCGGTTCGCAGCCCGAGCACCGCGTGAAGGTGCGCGTGATCAACGAATTCGCATGGCACAATCTGTTTATCC
GCACGCTATTGTGCCGCCCGATGGCAAGCGAGCTGGACGGGTTCGCGCCGGAATACACCATCATCGACCTGCCGAGCTTC
CAGGCCGATCCGGAGCGCCACGGTACGCGCAGCGAGACCGTGATCGCCGTCAATCTCAGCGAGAAGCTGATCCTGATCGG
CGGCACCAAATATGCCGGCGAGATGAAGAAAAGCGTCTTCGGCATTCTCAATTACCTGCTGCCGGCCAAGGGCGTGATGC
CGATGCATTGCAGCGCGAATATCGGCCCCGATGGCAAAACAGCGGTTTTCTTCGGTCTTTCCGGCACCGGCAAGACCACG
CTTTCCGCCGATGCCAGCCGCACGCTGATCGGCGACGACGAGCACGGCTGGTCTGATACGGCGGTCTTCAATTTCGAAGG
CGGCTGCTACGCCAAGATGATCCGCCTCAGCGAAGAGGCCGAGCCGGAAATCTACGCCACCACGCGCATGTTCGGCACGG
TGCTGGAAAACGTTGTGATGGACGAGGAAACCCGCGAGCTCGATTTCGACGACAATACGCTCGCCGAAAACACCCGCGGC
GCCTATCCGATCGACTATATCCCGAACACCAGCGAGGAAAACCTCGGCCCGGTGCCGAGCAATGTCGTGATGCTGACCGC
CGATGCCTTCGGCGTGTTGCCTCCGATCGCTCGGCTGACGCCCGACCAGGCGATGTATCACTTCCTTTCCGGCTACACCG
CCAAGGTCGCCGGGACCGAGATCGGCGTGACCGAGCCGGAAGCCACCTTCAGCACCTGCTTCGGCGCACCGTTCATGCCG
CGCCATCCGAGCGTTTACGGTAACCTCCTGAAAGAACGCATCGCCAAGGGCGGGGTGCAGTGCTGGCTGCTCAACACCGG
CTGGACCGGCGGCAAGTACGGCGTTGGCAACCGCATGCCGATCAAGGCCACCCGCGCGCTGCTCAATGCCGCGCTCGATG
GCAAGATGGACGATGTCGAGTTCCGCAAGGACCCGAACTTCGGCTTCGACGTGCCGGTCAGCGTTCCGGCGCTTGCAGAA
GCCGGCATCGACCAGACGATCCTCGATCCGCGCAGCACCTGGGCGGACAAGGAAGAATACGACGCGACTGCGCAGAAGCT
GGTGCAGCTGTTCGTCGACAATTTCGAGCCGTTCGCGGCGCATGTCGATGAAGGCGTCCGGCAGGCGGCGCCGAGCAGCG
AGCCGCAGGCCGCCTAG

Upstream 100 bases:

>100_bases
TGTATATGCGCGGTTTAGCAGACCATGCATAGGTATGCACCGAGGCTCCCGGCCTCGCACGAGACCCCCACGTCTTACGG
TTTATCAGGAGCTTACGAGC

Downstream 100 bases:

>100_bases
AGCCGCGCTTTCCCAGTTGGAGAGGAGGGCCCCGCACTGCGATCATATGCGGGCGGGGCCTTTTTTCGCAGGTGGAGCGT
TCGGTGGCCGAAGCGGTCGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTALSTSLADQGISTNATIHPNLGTAQLVEHAIDNGEGRLSKYGSLVVETGKHTGRSAKDKFIVRDSETEDTVWWDNNAS
IDPEHFAALKEDFLKAVAEKDTLYVADLFGGSQPEHRVKVRVINEFAWHNLFIRTLLCRPMASELDGFAPEYTIIDLPSF
QADPERHGTRSETVIAVNLSEKLILIGGTKYAGEMKKSVFGILNYLLPAKGVMPMHCSANIGPDGKTAVFFGLSGTGKTT
LSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSEEAEPEIYATTRMFGTVLENVVMDEETRELDFDDNTLAENTRG
AYPIDYIPNTSEENLGPVPSNVVMLTADAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEIGVTEPEATFSTCFGAPFMP
RHPSVYGNLLKERIAKGGVQCWLLNTGWTGGKYGVGNRMPIKATRALLNAALDGKMDDVEFRKDPNFGFDVPVSVPALAE
AGIDQTILDPRSTWADKEEYDATAQKLVQLFVDNFEPFAAHVDEGVRQAAPSSEPQAA

Sequences:

>Translated_538_residues
MTALSTSLADQGISTNATIHPNLGTAQLVEHAIDNGEGRLSKYGSLVVETGKHTGRSAKDKFIVRDSETEDTVWWDNNAS
IDPEHFAALKEDFLKAVAEKDTLYVADLFGGSQPEHRVKVRVINEFAWHNLFIRTLLCRPMASELDGFAPEYTIIDLPSF
QADPERHGTRSETVIAVNLSEKLILIGGTKYAGEMKKSVFGILNYLLPAKGVMPMHCSANIGPDGKTAVFFGLSGTGKTT
LSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSEEAEPEIYATTRMFGTVLENVVMDEETRELDFDDNTLAENTRG
AYPIDYIPNTSEENLGPVPSNVVMLTADAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEIGVTEPEATFSTCFGAPFMP
RHPSVYGNLLKERIAKGGVQCWLLNTGWTGGKYGVGNRMPIKATRALLNAALDGKMDDVEFRKDPNFGFDVPVSVPALAE
AGIDQTILDPRSTWADKEEYDATAQKLVQLFVDNFEPFAAHVDEGVRQAAPSSEPQAA
>Mature_537_residues
TALSTSLADQGISTNATIHPNLGTAQLVEHAIDNGEGRLSKYGSLVVETGKHTGRSAKDKFIVRDSETEDTVWWDNNASI
DPEHFAALKEDFLKAVAEKDTLYVADLFGGSQPEHRVKVRVINEFAWHNLFIRTLLCRPMASELDGFAPEYTIIDLPSFQ
ADPERHGTRSETVIAVNLSEKLILIGGTKYAGEMKKSVFGILNYLLPAKGVMPMHCSANIGPDGKTAVFFGLSGTGKTTL
SADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSEEAEPEIYATTRMFGTVLENVVMDEETRELDFDDNTLAENTRGA
YPIDYIPNTSEENLGPVPSNVVMLTADAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEIGVTEPEATFSTCFGAPFMPR
HPSVYGNLLKERIAKGGVQCWLLNTGWTGGKYGVGNRMPIKATRALLNAALDGKMDDVEFRKDPNFGFDVPVSVPALAEA
GIDQTILDPRSTWADKEEYDATAQKLVQLFVDNFEPFAAHVDEGVRQAAPSSEPQAA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=495, Percent_Identity=48.4848484848485, Blast_Score=467, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322950, Length=531, Percent_Identity=43.5028248587571, Blast_Score=434, Evalue=1e-122,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58648; Mature: 58517

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALSTSLADQGISTNATIHPNLGTAQLVEHAIDNGEGRLSKYGSLVVETGKHTGRSAKD
CCCCCCHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCCHHHHHCCEEEECCCCCCCCCCC
KFIVRDSETEDTVWWDNNASIDPEHFAALKEDFLKAVAEKDTLYVADLFGGSQPEHRVKV
CEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEE
RVINEFAWHNLFIRTLLCRPMASELDGFAPEYTIIDLPSFQADPERHGTRSETVIAVNLS
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCCCCEEEEEECC
EKLILIGGTKYAGEMKKSVFGILNYLLPAKGVMPMHCSANIGPDGKTAVFFGLSGTGKTT
CEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCCCCEE
LSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSEEAEPEIYATTRMFGTVLENVVM
EECCCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHC
DEETRELDFDDNTLAENTRGAYPIDYIPNTSEENLGPVPSNVVMLTADAFGVLPPIARLT
CCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCCHHHCC
PDQAMYHFLSGYTAKVAGTEIGVTEPEATFSTCFGAPFMPRHPSVYGNLLKERIAKGGVQ
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
CWLLNTGWTGGKYGVGNRMPIKATRALLNAALDGKMDDVEFRKDPNFGFDVPVSVPALAE
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHH
AGIDQTILDPRSTWADKEEYDATAQKLVQLFVDNFEPFAAHVDEGVRQAAPSSEPQAA
CCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TALSTSLADQGISTNATIHPNLGTAQLVEHAIDNGEGRLSKYGSLVVETGKHTGRSAKD
CCCCCHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCCHHHHHCCEEEECCCCCCCCCCC
KFIVRDSETEDTVWWDNNASIDPEHFAALKEDFLKAVAEKDTLYVADLFGGSQPEHRVKV
CEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEE
RVINEFAWHNLFIRTLLCRPMASELDGFAPEYTIIDLPSFQADPERHGTRSETVIAVNLS
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCCCCEEEEEECC
EKLILIGGTKYAGEMKKSVFGILNYLLPAKGVMPMHCSANIGPDGKTAVFFGLSGTGKTT
CEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCCCCEE
LSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSEEAEPEIYATTRMFGTVLENVVM
EECCCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHC
DEETRELDFDDNTLAENTRGAYPIDYIPNTSEENLGPVPSNVVMLTADAFGVLPPIARLT
CCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCCHHHCC
PDQAMYHFLSGYTAKVAGTEIGVTEPEATFSTCFGAPFMPRHPSVYGNLLKERIAKGGVQ
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
CWLLNTGWTGGKYGVGNRMPIKATRALLNAALDGKMDDVEFRKDPNFGFDVPVSVPALAE
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHH
AGIDQTILDPRSTWADKEEYDATAQKLVQLFVDNFEPFAAHVDEGVRQAAPSSEPQAA
CCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA