Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 85373237

GI number: 85373237

Start: 455135

End: 456112

Strand: Direct

Name: ansA [H]

Synonym: ELI_02050

Alternate gene names: 85373237

Gene position: 455135-456112 (Clockwise)

Preceding gene: 85373236

Following gene: 85373241

Centisome position: 14.91

GC content: 65.95

Gene sequence:

>978_bases
ATGACGAAGCGGCTCACTGTCCTCGCCACCGGCGGCACTATCGCAGGGATCGCGGGATCGGCGATCGCCAAGGACTACAC
CTCGGGCCAGATTTCGATTGAAGAATATCTTGAACAGGTCGGCGGGCTGGGCCTCGCGGCGGAACTGTCCGGCACGCAAA
TCGCCAACATCGATTCGGCAGATATCGGCCCGGAGGTTTGGGGGCCCCTGCATGCGGCAATCGTCGATGCGCTGGCGGAC
GAAACGTGCGACGGGGTGATCGTGACGCACGGCACCGACACGCTGGAAGAGACGGCGTTCCTGCTCGACCTCACGCTGCC
TGCGACCAAGCCGGTCGTGCTGGTCGGCGCGATGCGGCCCGCCGATGCGGTGGGTTACGACGGGCTCAGGAACTTTGCCA
ACGCCGTAAACGTCGCGGGCGACGACGATGCGGCGGGGCGCGGTGTGCTCGTGGTCATGGGCGACCGCGTGTTTGGCGCG
CGCGACGTCCGCAAGATGCGCACCCGCGGCACCGATGCTTTTCGCGGCTTTCCGCGCGAGTCGGTCGGGCTGGTGAGCCC
GTCCTCCCTCGAATGGTTCGGCGCGCCGTGGCGGCACGGGGACAAGGCGCATTTCGGCTGGTACGATGATCTGCCCGAGG
TCGCGATTATCTACGCGCATGCAGGGCTGACGGCCGCACAGGCTGTGCGACAGATCGGCTCTGATATACGCGGCGTCGTG
ATCGCGGGTGTTGGCGAGGGCAATATGCCGGAGACGGTTCGGCAGATTTTGGTCGCCAAATGCGCGCAGGGCCTTGCCGT
CGTCCGCGCCAGTCGCGCCGATGAAGGGCTGGTCGACCGCGAGCCGGAAGACGATGCCAACGGTTTCGTTGCCGCGCGCG
CGCTCAATCCGCAAAAGGCCCGGATCCTGCTGCAATTACTGCTCGCAGAGGGTGTGTCGGACCCTGCCGCGATCCAGCGT
GCATTCGATGGAAGCTAG

Upstream 100 bases:

>100_bases
CTGAGCTAATCGCCCTACTGCATCGCAGCAGAGCGCCCACATAGCGCGCGAGGGTTCAAAATCAATTGCCAATCGTGCGG
GCGGGGGTAGGGACACGGCC

Downstream 100 bases:

>100_bases
GCGAAACACCCCCAAACGTTCGCCCTCAGCCTGTCGAAGGGCTGTCCTTCTTCTGCACGGCATCTCCAAAAGAAGTGCAG
TGCTTCGACAGGCTCAGCAC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTKRLTVLATGGTIAGIAGSAIAKDYTSGQISIEEYLEQVGGLGLAAELSGTQIANIDSADIGPEVWGPLHAAIVDALAD
ETCDGVIVTHGTDTLEETAFLLDLTLPATKPVVLVGAMRPADAVGYDGLRNFANAVNVAGDDDAAGRGVLVVMGDRVFGA
RDVRKMRTRGTDAFRGFPRESVGLVSPSSLEWFGAPWRHGDKAHFGWYDDLPEVAIIYAHAGLTAAQAVRQIGSDIRGVV
IAGVGEGNMPETVRQILVAKCAQGLAVVRASRADEGLVDREPEDDANGFVAARALNPQKARILLQLLLAEGVSDPAAIQR
AFDGS

Sequences:

>Translated_325_residues
MTKRLTVLATGGTIAGIAGSAIAKDYTSGQISIEEYLEQVGGLGLAAELSGTQIANIDSADIGPEVWGPLHAAIVDALAD
ETCDGVIVTHGTDTLEETAFLLDLTLPATKPVVLVGAMRPADAVGYDGLRNFANAVNVAGDDDAAGRGVLVVMGDRVFGA
RDVRKMRTRGTDAFRGFPRESVGLVSPSSLEWFGAPWRHGDKAHFGWYDDLPEVAIIYAHAGLTAAQAVRQIGSDIRGVV
IAGVGEGNMPETVRQILVAKCAQGLAVVRASRADEGLVDREPEDDANGFVAARALNPQKARILLQLLLAEGVSDPAAIQR
AFDGS
>Mature_324_residues
TKRLTVLATGGTIAGIAGSAIAKDYTSGQISIEEYLEQVGGLGLAAELSGTQIANIDSADIGPEVWGPLHAAIVDALADE
TCDGVIVTHGTDTLEETAFLLDLTLPATKPVVLVGAMRPADAVGYDGLRNFANAVNVAGDDDAAGRGVLVVMGDRVFGAR
DVRKMRTRGTDAFRGFPRESVGLVSPSSLEWFGAPWRHGDKAHFGWYDDLPEVAIIYAHAGLTAAQAVRQIGSDIRGVVI
AGVGEGNMPETVRQILVAKCAQGLAVVRASRADEGLVDREPEDDANGFVAARALNPQKARILLQLLLAEGVSDPAAIQRA
FDGS

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=325, Percent_Identity=41.8461538461538, Blast_Score=208, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323189, Length=320, Percent_Identity=35.625, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323187, Length=320, Percent_Identity=35.625, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323186, Length=320, Percent_Identity=35.625, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323184, Length=320, Percent_Identity=35.625, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6320527, Length=338, Percent_Identity=31.0650887573964, Blast_Score=155, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33981; Mature: 33850

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRLTVLATGGTIAGIAGSAIAKDYTSGQISIEEYLEQVGGLGLAAELSGTQIANIDSA
CCCEEEEEECCCCEEHHCCHHHHHCCCCCCEEHHHHHHHHCCCCEEEECCCCEEECCCCC
DIGPEVWGPLHAAIVDALADETCDGVIVTHGTDTLEETAFLLDLTLPATKPVVLVGAMRP
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHEEEEEEECCCCCCEEEEECCCC
ADAVGYDGLRNFANAVNVAGDDDAAGRGVLVVMGDRVFGARDVRKMRTRGTDAFRGFPRE
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHCCCCC
SVGLVSPSSLEWFGAPWRHGDKAHFGWYDDLPEVAIIYAHAGLTAAQAVRQIGSDIRGVV
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCEEE
IAGVGEGNMPETVRQILVAKCAQGLAVVRASRADEGLVDREPEDDANGFVAARALNPQKA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
RILLQLLLAEGVSDPAAIQRAFDGS
HHHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
TKRLTVLATGGTIAGIAGSAIAKDYTSGQISIEEYLEQVGGLGLAAELSGTQIANIDSA
CCEEEEEECCCCEEHHCCHHHHHCCCCCCEEHHHHHHHHCCCCEEEECCCCEEECCCCC
DIGPEVWGPLHAAIVDALADETCDGVIVTHGTDTLEETAFLLDLTLPATKPVVLVGAMRP
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHEEEEEEECCCCCCEEEEECCCC
ADAVGYDGLRNFANAVNVAGDDDAAGRGVLVVMGDRVFGARDVRKMRTRGTDAFRGFPRE
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHCCCCC
SVGLVSPSSLEWFGAPWRHGDKAHFGWYDDLPEVAIIYAHAGLTAAQAVRQIGSDIRGVV
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCEEE
IAGVGEGNMPETVRQILVAKCAQGLAVVRASRADEGLVDREPEDDANGFVAARALNPQKA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
RILLQLLLAEGVSDPAAIQRAFDGS
HHHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]