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The map label for this gene is queF [H]

Identifier: 85373211

GI number: 85373211

Start: 428812

End: 429294

Strand: Direct

Name: queF [H]

Synonym: ELI_01920

Alternate gene names: 85373211

Gene position: 428812-429294 (Clockwise)

Preceding gene: 85373210

Following gene: 85373212

Centisome position: 14.05

GC content: 64.8

Gene sequence:

>483_bases
ATGCGCGGCATGAGTGACACACCGACACCAGCCGAGGGCGCTCCGCAGTTTCTCGGCAAGCAGACCGCGCTGCCCGCTTC
GCCGGAAGATGCGGTGCTCGATTACGTGCCCAATCCGCGCCCCGGCGCGCTCTATCTCGTACGCTTTGCCGCGCCGGAGT
TCACATCGTTGTGCCCGGTCACCGGCGCACCCGACTTCGCGCATCTCGTCATCGATTATGCCCCGGGCGACACGGTCGTC
GAATCCAAGAGCCTCAAGCTCTTTCTCGGCTCTTTCCGCAACCACAACGGCTTCCATGAAGACGTGACGGTCGGCATCGG
CCAGCGCCTCAACGAAGAGATGCGACCGCGATGGCTCAGGATCGGCGGTTACTGGTATCCGCGCGGCGGCATCCCCATCG
ACGTCTTTTGGCAGAGCGGACCTTCCCCCGAAGGGCTTTGGGTGCCGGATCAGGGCGTTGCGGGCTATCGCGGGCGCGGT
TGA

Upstream 100 bases:

>100_bases
TCTGGTACGGACTGCGTGGTCCTGTGCGACGGCGCGCTGAATAGGCTCTTAATCGCCACGGTCAATCGGCTGGCTTGTGC
TTCGCGCAAGATCGCGGCAG

Downstream 100 bases:

>100_bases
TCGGTTAGCGTGCCTGCCGTGCATGCTGGGGGAGGCATGATGGGCGGACAATCGAGACACGATGCGTAAAGCGGCGATCC
TCATCGGGATCATAGTGGCG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRGMSDTPTPAEGAPQFLGKQTALPASPEDAVLDYVPNPRPGALYLVRFAAPEFTSLCPVTGAPDFAHLVIDYAPGDTVV
ESKSLKLFLGSFRNHNGFHEDVTVGIGQRLNEEMRPRWLRIGGYWYPRGGIPIDVFWQSGPSPEGLWVPDQGVAGYRGRG

Sequences:

>Translated_160_residues
MRGMSDTPTPAEGAPQFLGKQTALPASPEDAVLDYVPNPRPGALYLVRFAAPEFTSLCPVTGAPDFAHLVIDYAPGDTVV
ESKSLKLFLGSFRNHNGFHEDVTVGIGQRLNEEMRPRWLRIGGYWYPRGGIPIDVFWQSGPSPEGLWVPDQGVAGYRGRG
>Mature_160_residues
MRGMSDTPTPAEGAPQFLGKQTALPASPEDAVLDYVPNPRPGALYLVRFAAPEFTSLCPVTGAPDFAHLVIDYAPGDTVV
ESKSLKLFLGSFRNHNGFHEDVTVGIGQRLNEEMRPRWLRIGGYWYPRGGIPIDVFWQSGPSPEGLWVPDQGVAGYRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 17469; Mature: 17469

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGMSDTPTPAEGAPQFLGKQTALPASPEDAVLDYVPNPRPGALYLVRFAAPEFTSLCPV
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCC
TGAPDFAHLVIDYAPGDTVVESKSLKLFLGSFRNHNGFHEDVTVGIGQRLNEEMRPRWLR
CCCCCCEEEEEEECCCCCEECCCCEEEEEECCCCCCCCCCCEEECCHHHCCHHHCCEEEE
IGGYWYPRGGIPIDVFWQSGPSPEGLWVPDQGVAGYRGRG
ECCEEECCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MRGMSDTPTPAEGAPQFLGKQTALPASPEDAVLDYVPNPRPGALYLVRFAAPEFTSLCPV
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCC
TGAPDFAHLVIDYAPGDTVVESKSLKLFLGSFRNHNGFHEDVTVGIGQRLNEEMRPRWLR
CCCCCCEEEEEEECCCCCEECCCCEEEEEECCCCCCCCCCCEEECCHHHCCHHHCCEEEE
IGGYWYPRGGIPIDVFWQSGPSPEGLWVPDQGVAGYRGRG
ECCEEECCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA