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The map label for this gene is penA [H]

Identifier: 85373180

GI number: 85373180

Start: 394634

End: 396391

Strand: Direct

Name: penA [H]

Synonym: ELI_01765

Alternate gene names: 85373180

Gene position: 394634-396391 (Clockwise)

Preceding gene: 85373179

Following gene: 85373181

Centisome position: 12.93

GC content: 66.15

Gene sequence:

>1758_bases
ATGAGTTCGCTTGCAGTCAGCACCGCCATCTCCTCCGGGCGCGTGCGGCTCGTTTCCGATCGTCAGGAAGCCCTGCTGGT
CGCGCGCATCCGCGTGCTGGTGGTGGCCGTCGGCTTTGCCTTGCTGGCGCTGATCGCGCTTCTGCGGATAGTCTTTCTCG
GCATCGGTGGCGGCACGACGGGGCCGACCACGCTGGAAGAGGCCTTGCTGCCGCCGCGCGGGGAAATCACCGACCGCAAC
GGCATCGCGCTCGCCCGTAACTATCCGGCGCGCGCACTGTGGTTCAATCCCGATGCGCTGGGCGAAGGCGAGGAGTCCCT
GGTCAAGTCGCCGGACAAGGTGGTTGCCGAACTGCTCGCGATCTTCCCCGATCTCGAGCAGGATCGCGTGCGCAAGATGC
TCGCCAGCGGCACCGCAGGCTATTTGCGCCGCCGTGTCCTGCCCGAGGAAGCCAACCGTGTGTTCGACATCGGCGAGGTC
GCGCTGGAACTGCCGCAGGAAACCGACCGCCACTATCCGCAAGGCATGCTCGGCGCCCACGTGCTCGGCTATGTCGACAG
CGATGGCGGCGGCAAGGTCGGCATGGAGCAAGTGCTCGACGACCGGCTGAGCAATCCCGTGCTGCGCGGCGAGGCATCGT
CGCTCTCGCTCGATATCCGCGTGCAGGGCGCGCTGGAAGACGAACTGCGCAAGGGCATGCTCTCGGTCAACGCGATGGGC
GCGGCGGGGATCGTGCTGGATGTCGACACCGGCGAGATCTTGGCCCTTGCGTCGCTGCCGGAATTCGATCCCAACGCCGT
GAAGGGCGAAGACCTGATCGTATCGGATGCCGCCTACAAGCGCGGCGAAATCCCGCGCGCCTTCAACCGCGTGACCAACC
AGGTGTACGAGCTCGGCTCCACCTTCAAGCCGCTGACGGTCGCGGCCGCGATCGATGCCGGCACAGTGCGCGATTTCGGC
AAGCGTTACGATGCCAGCGGGGTCGCGATCGACGGATTTACCATCCGCGACAGCCACGATCTCGGCGCGAGCCTCAACAT
CCCGCAGATGCTGATCCATTCGTCCAACACCGTGACCGCACGCGTGTCCGACGAACTGGGCGAGGCCAAGCTGCGGCAGA
CCTTCGAAGACCTCGGCATGCACGAGCGGCCTTATATCGAGCTGCCCGCGCGCGGCCATCCGCTGTGGCAGACCAGCAAG
TGGGGTCGCCTGAAGACGATGAACGTCGGGTTCGGCCACGGCATCGCCGTCACGCCGCTGCACCTGGCCAGCGCCTATGC
CGCGATGGTCAATGGCGGCATCTGGCGTCCGGCGACGCTGCACAGGATCGAACCGGGCATGGCGCCCAAGGGTCGACGCG
TGTTCAAGGAATCGACTTCCAGCAGGATGCGCCAGTTGCTGCGCATGATCGCGCTCTACGGCACCGGCAAGAATGCCGAC
GCGGCGGGCTATCGGGTCGGCGGCAAGACCGGCTCGGCGGAGAAACCGGGTGTCGGTGGCTATCGTCGCAAGAGCTTGGT
TTCGACCTTTGCCGCAGTGTTCCCGATGGACAACCCGCGCTTCGTGGTGATCGCGATGCTGGACGAGCCGAAGGGAACGA
CTGCGAGTTCGTTCCAGCGCACCGCTGCATGGAACGCAGCGCCGATTGTCGGCAAGCTGGTGCCCCGCATCGGCCCGATC
CTCGGCGTGCGCCCCGACATGACCCGCGACGTCGATATCTCCGACCTCAAGCGCCTTGTGCCGGGAGCGGCCGAGTGA

Upstream 100 bases:

>100_bases
CCCGCGGCGAAGATCGACCTTCGCTCGCCGAACGCCTCGGCATGCAGGTCGACCTGCGCAGCACATCGCCCGACTTCACG
CCCGCCGCAAAGGTGTCCGA

Downstream 100 bases:

>100_bases
AGCTTGCCCGGCTGGCAGAAGCGGCAGGCATTGCCATCGAAGGCGGCGAGGACATCGCCGTCTCGGGTTTTGCCATCGAC
CATCGCAAGGTCGCGCCGGG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSSLAVSTAISSGRVRLVSDRQEALLVARIRVLVVAVGFALLALIALLRIVFLGIGGGTTGPTTLEEALLPPRGEITDRN
GIALARNYPARALWFNPDALGEGEESLVKSPDKVVAELLAIFPDLEQDRVRKMLASGTAGYLRRRVLPEEANRVFDIGEV
ALELPQETDRHYPQGMLGAHVLGYVDSDGGGKVGMEQVLDDRLSNPVLRGEASSLSLDIRVQGALEDELRKGMLSVNAMG
AAGIVLDVDTGEILALASLPEFDPNAVKGEDLIVSDAAYKRGEIPRAFNRVTNQVYELGSTFKPLTVAAAIDAGTVRDFG
KRYDASGVAIDGFTIRDSHDLGASLNIPQMLIHSSNTVTARVSDELGEAKLRQTFEDLGMHERPYIELPARGHPLWQTSK
WGRLKTMNVGFGHGIAVTPLHLASAYAAMVNGGIWRPATLHRIEPGMAPKGRRVFKESTSSRMRQLLRMIALYGTGKNAD
AAGYRVGGKTGSAEKPGVGGYRRKSLVSTFAAVFPMDNPRFVVIAMLDEPKGTTASSFQRTAAWNAAPIVGKLVPRIGPI
LGVRPDMTRDVDISDLKRLVPGAAE

Sequences:

>Translated_585_residues
MSSLAVSTAISSGRVRLVSDRQEALLVARIRVLVVAVGFALLALIALLRIVFLGIGGGTTGPTTLEEALLPPRGEITDRN
GIALARNYPARALWFNPDALGEGEESLVKSPDKVVAELLAIFPDLEQDRVRKMLASGTAGYLRRRVLPEEANRVFDIGEV
ALELPQETDRHYPQGMLGAHVLGYVDSDGGGKVGMEQVLDDRLSNPVLRGEASSLSLDIRVQGALEDELRKGMLSVNAMG
AAGIVLDVDTGEILALASLPEFDPNAVKGEDLIVSDAAYKRGEIPRAFNRVTNQVYELGSTFKPLTVAAAIDAGTVRDFG
KRYDASGVAIDGFTIRDSHDLGASLNIPQMLIHSSNTVTARVSDELGEAKLRQTFEDLGMHERPYIELPARGHPLWQTSK
WGRLKTMNVGFGHGIAVTPLHLASAYAAMVNGGIWRPATLHRIEPGMAPKGRRVFKESTSSRMRQLLRMIALYGTGKNAD
AAGYRVGGKTGSAEKPGVGGYRRKSLVSTFAAVFPMDNPRFVVIAMLDEPKGTTASSFQRTAAWNAAPIVGKLVPRIGPI
LGVRPDMTRDVDISDLKRLVPGAAE
>Mature_584_residues
SSLAVSTAISSGRVRLVSDRQEALLVARIRVLVVAVGFALLALIALLRIVFLGIGGGTTGPTTLEEALLPPRGEITDRNG
IALARNYPARALWFNPDALGEGEESLVKSPDKVVAELLAIFPDLEQDRVRKMLASGTAGYLRRRVLPEEANRVFDIGEVA
LELPQETDRHYPQGMLGAHVLGYVDSDGGGKVGMEQVLDDRLSNPVLRGEASSLSLDIRVQGALEDELRKGMLSVNAMGA
AGIVLDVDTGEILALASLPEFDPNAVKGEDLIVSDAAYKRGEIPRAFNRVTNQVYELGSTFKPLTVAAAIDAGTVRDFGK
RYDASGVAIDGFTIRDSHDLGASLNIPQMLIHSSNTVTARVSDELGEAKLRQTFEDLGMHERPYIELPARGHPLWQTSKW
GRLKTMNVGFGHGIAVTPLHLASAYAAMVNGGIWRPATLHRIEPGMAPKGRRVFKESTSSRMRQLLRMIALYGTGKNADA
AGYRVGGKTGSAEKPGVGGYRRKSLVSTFAAVFPMDNPRFVVIAMLDEPKGTTASSFQRTAAWNAAPIVGKLVPRIGPIL
GVRPDMTRDVDISDLKRLVPGAAE

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=518, Percent_Identity=28.3783783783784, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786854, Length=643, Percent_Identity=24.4167962674961, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 62910; Mature: 62779

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLAVSTAISSGRVRLVSDRQEALLVARIRVLVVAVGFALLALIALLRIVFLGIGGGTT
CCCCEEHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
GPTTLEEALLPPRGEITDRNGIALARNYPARALWFNPDALGEGEESLVKSPDKVVAELLA
CCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHCCHHHHHHHHHH
IFPDLEQDRVRKMLASGTAGYLRRRVLPEEANRVFDIGEVALELPQETDRHYPQGMLGAH
HCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHEEEHHHHHHHCCHHHHCCCCCCHHHHH
VLGYVDSDGGGKVGMEQVLDDRLSNPVLRGEASSLSLDIRVQGALEDELRKGMLSVNAMG
EEEEEECCCCCCCCHHHHHHHHHCCCEECCCCCCEEEEEEEECCHHHHHHHHHHEEECCC
AAGIVLDVDTGEILALASLPEFDPNAVKGEDLIVSDAAYKRGEIPRAFNRVTNQVYELGS
CCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHCC
TFKPLTVAAAIDAGTVRDFGKRYDASGVAIDGFTIRDSHDLGASLNIPQMLIHSSNTVTA
CCCCEEEEEECCCCHHHHHHHHCCCCCEEEECEEEECCCCCCCCCCCCHHEEECCCEEEE
RVSDELGEAKLRQTFEDLGMHERPYIELPARGHPLWQTSKWGRLKTMNVGFGHGIAVTPL
EECHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEECHH
HLASAYAAMVNGGIWRPATLHRIEPGMAPKGRRVFKESTSSRMRQLLRMIALYGTGKNAD
HHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AAGYRVGGKTGSAEKPGVGGYRRKSLVSTFAAVFPMDNPRFVVIAMLDEPKGTTASSFQR
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHH
TAAWNAAPIVGKLVPRIGPILGVRPDMTRDVDISDLKRLVPGAAE
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
SSLAVSTAISSGRVRLVSDRQEALLVARIRVLVVAVGFALLALIALLRIVFLGIGGGTT
CCCEEHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
GPTTLEEALLPPRGEITDRNGIALARNYPARALWFNPDALGEGEESLVKSPDKVVAELLA
CCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHCCHHHHHHHHHH
IFPDLEQDRVRKMLASGTAGYLRRRVLPEEANRVFDIGEVALELPQETDRHYPQGMLGAH
HCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHEEEHHHHHHHCCHHHHCCCCCCHHHHH
VLGYVDSDGGGKVGMEQVLDDRLSNPVLRGEASSLSLDIRVQGALEDELRKGMLSVNAMG
EEEEEECCCCCCCCHHHHHHHHHCCCEECCCCCCEEEEEEEECCHHHHHHHHHHEEECCC
AAGIVLDVDTGEILALASLPEFDPNAVKGEDLIVSDAAYKRGEIPRAFNRVTNQVYELGS
CCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHCC
TFKPLTVAAAIDAGTVRDFGKRYDASGVAIDGFTIRDSHDLGASLNIPQMLIHSSNTVTA
CCCCEEEEEECCCCHHHHHHHHCCCCCEEEECEEEECCCCCCCCCCCCHHEEECCCEEEE
RVSDELGEAKLRQTFEDLGMHERPYIELPARGHPLWQTSKWGRLKTMNVGFGHGIAVTPL
EECHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEECHH
HLASAYAAMVNGGIWRPATLHRIEPGMAPKGRRVFKESTSSRMRQLLRMIALYGTGKNAD
HHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AAGYRVGGKTGSAEKPGVGGYRRKSLVSTFAAVFPMDNPRFVVIAMLDEPKGTTASSFQR
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHH
TAAWNAAPIVGKLVPRIGPILGVRPDMTRDVDISDLKRLVPGAAE
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]