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The map label for this gene is mraW

Identifier: 85373178

GI number: 85373178

Start: 393133

End: 394080

Strand: Direct

Name: mraW

Synonym: ELI_01755

Alternate gene names: 85373178

Gene position: 393133-394080 (Clockwise)

Preceding gene: 85373177

Following gene: 85373179

Centisome position: 12.88

GC content: 67.62

Gene sequence:

>948_bases
ATGAGCGGCGCGGCTGCCCATGTCCCCGTCCTGCTTGACGAAGTCGTCGCTGCGCTGGATCCGCAGCCGGGCGATGTGCT
GGTCGATGCGACTTTCGGGGCCGGCGGTTACACTCGCGCGCTGCTCGACAGCGGCGCGACGGTCCACGCTTTTGATCGCG
ACCCCGATGCGATCGCGGCCGGATCGCAATGGCCAGAGACGAAGGAGGAGCCGCCGCGGCTGGTCCTCCATCCGCGCCGC
TTCTCCGAGATGGTGCACGCGCTGGCCGAAGCCGGCGTGGCGCAGGTCGACGGCGTCGCGATGGATATCGGCGTCTCTTC
GATGCAGCTCGACCAGGCCCAACGAGGCTTCGCATTCTCCGCCGACGGCCCGCTCGACATGCGCATGAGCCAGCATGGCC
CCAGCGCTGCCGACTTCCTCAATTCGGCGGAAGCCGAAGAAATTGCCGACGTGCTCTACCAGTATGGTGAAGAGCGCCAG
TCGCGCCGCGTCGCCCGCGCCATCATTGCTGCGCGCCCGCTGGAGACGACCGGCGATCTCGCCCGCGTCGTGCGCAAGGC
TTTGGGTTATCGCGTGGGCGCGCCGAAAGACCCGGCGACGCGCACCTTCCAGGCAGTCCGTATCCATGTGAATGGCGAGC
TCGACGAGCTGCGCGAAGGCCTCCATGCCGCCGAGCATCTGCTCAGGAGCGGCGGGCGGCTCGCGGTCGTCAGTTTCCAT
TCGCTCGAAGATCGTATCGTGAAGCGCTTTCTCCAGAGCGCTTCCGGAAAGGCCGGCGGCACGTCCCGCTATCTTCCCCA
GACCGATGTGTCGCCGGCCATCTTTTCCGACATCTCCAAGGCGATCCGCCCGTCCGAGGCGGAAATCGCCCGCAATCCGC
GCGCGCGGTCTTCGACGCTGCGTCACGCCACTCGCACCGATGCGCCGCCGCGCAAGGAGGCCGCATGA

Upstream 100 bases:

>100_bases
GGAACCCGGCCGAGCTCGATTGCATGGGTGACGATTGGGCGGCAGCCAAGGCGGCCTGCGCCGCGCTCGTCGCCGAAGCC
GAAACGAAAGCGAGAAAGAA

Downstream 100 bases:

>100_bases
TCGTCGGCGGCAGCAGGTTGAGGCAGATCGGCTGGGCAGCTGCGCTGGGCGTCGTCTTTGCGCTGTTCGTGGCGCTCACT
TTCAGGGTGAACGCGGTGAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSGAAAHVPVLLDEVVAALDPQPGDVLVDATFGAGGYTRALLDSGATVHAFDRDPDAIAAGSQWPETKEEPPRLVLHPRR
FSEMVHALAEAGVAQVDGVAMDIGVSSMQLDQAQRGFAFSADGPLDMRMSQHGPSAADFLNSAEAEEIADVLYQYGEERQ
SRRVARAIIAARPLETTGDLARVVRKALGYRVGAPKDPATRTFQAVRIHVNGELDELREGLHAAEHLLRSGGRLAVVSFH
SLEDRIVKRFLQSASGKAGGTSRYLPQTDVSPAIFSDISKAIRPSEAEIARNPRARSSTLRHATRTDAPPRKEAA

Sequences:

>Translated_315_residues
MSGAAAHVPVLLDEVVAALDPQPGDVLVDATFGAGGYTRALLDSGATVHAFDRDPDAIAAGSQWPETKEEPPRLVLHPRR
FSEMVHALAEAGVAQVDGVAMDIGVSSMQLDQAQRGFAFSADGPLDMRMSQHGPSAADFLNSAEAEEIADVLYQYGEERQ
SRRVARAIIAARPLETTGDLARVVRKALGYRVGAPKDPATRTFQAVRIHVNGELDELREGLHAAEHLLRSGGRLAVVSFH
SLEDRIVKRFLQSASGKAGGTSRYLPQTDVSPAIFSDISKAIRPSEAEIARNPRARSSTLRHATRTDAPPRKEAA
>Mature_314_residues
SGAAAHVPVLLDEVVAALDPQPGDVLVDATFGAGGYTRALLDSGATVHAFDRDPDAIAAGSQWPETKEEPPRLVLHPRRF
SEMVHALAEAGVAQVDGVAMDIGVSSMQLDQAQRGFAFSADGPLDMRMSQHGPSAADFLNSAEAEEIADVLYQYGEERQS
RRVARAIIAARPLETTGDLARVVRKALGYRVGAPKDPATRTFQAVRIHVNGELDELREGLHAAEHLLRSGGRLAVVSFHS
LEDRIVKRFLQSASGKAGGTSRYLPQTDVSPAIFSDISKAIRPSEAEIARNPRARSSTLRHATRTDAPPRKEAA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=40.5247813411079, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=42.7083333333333, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=44.9367088607595, Blast_Score=222, Evalue=3e-59,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=40.2985074626866, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=40.2985074626866, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_ERYLH (Q2NCZ8)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457240.1
- ProteinModelPortal:   Q2NCZ8
- SMR:   Q2NCZ8
- STRING:   Q2NCZ8
- GeneID:   3869317
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01755
- NMPDR:   fig|314225.3.peg.2739
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- PhylomeDB:   Q2NCZ8
- ProtClustDB:   PRK00050
- BioCyc:   ELIT314225:ELI_01755-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33808; Mature: 33677

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAAAHVPVLLDEVVAALDPQPGDVLVDATFGAGGYTRALLDSGATVHAFDRDPDAIAA
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCC
GSQWPETKEEPPRLVLHPRRFSEMVHALAEAGVAQVDGVAMDIGVSSMQLDQAQRGFAFS
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCHHHHCCEEEECCCCHHHHHHHHCCCEEC
ADGPLDMRMSQHGPSAADFLNSAEAEEIADVLYQYGEERQSRRVARAIIAARPLETTGDL
CCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ARVVRKALGYRVGAPKDPATRTFQAVRIHVNGELDELREGLHAAEHLLRSGGRLAVVSFH
HHHHHHHHCCCCCCCCCCHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEHH
SLEDRIVKRFLQSASGKAGGTSRYLPQTDVSPAIFSDISKAIRPSEAEIARNPRARSSTL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHH
RHATRTDAPPRKEAA
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
SGAAAHVPVLLDEVVAALDPQPGDVLVDATFGAGGYTRALLDSGATVHAFDRDPDAIAA
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCC
GSQWPETKEEPPRLVLHPRRFSEMVHALAEAGVAQVDGVAMDIGVSSMQLDQAQRGFAFS
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCHHHHCCEEEECCCCHHHHHHHHCCCEEC
ADGPLDMRMSQHGPSAADFLNSAEAEEIADVLYQYGEERQSRRVARAIIAARPLETTGDL
CCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ARVVRKALGYRVGAPKDPATRTFQAVRIHVNGELDELREGLHAAEHLLRSGGRLAVVSFH
HHHHHHHHCCCCCCCCCCHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEHH
SLEDRIVKRFLQSASGKAGGTSRYLPQTDVSPAIFSDISKAIRPSEAEIARNPRARSSTL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHH
RHATRTDAPPRKEAA
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA