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The map label for this gene is clpB [H]

Identifier: 85373114

GI number: 85373114

Start: 323867

End: 326446

Strand: Direct

Name: clpB [H]

Synonym: ELI_01435

Alternate gene names: 85373114

Gene position: 323867-326446 (Clockwise)

Preceding gene: 85373113

Following gene: 85373115

Centisome position: 10.61

GC content: 63.84

Gene sequence:

>2580_bases
ATGAATCTCGAGAAATTTACCGACCGTGCCAAGGGCTTTCTGCAGGCCGCGCAGACGGTCGCGATCCGTATGAGCCACCA
GCGCATCACGCCGGCACATTTGCTCAAGGCGATGATCGAGGATCCGGAAGGCATGGCCTCCGGGCTGATAGCGCGTGCCG
GCGGCAATCCGCGCACGGTCGAAGACGAAGTCGATGCGGCGCTGGGCAAGATTCCGGCGGTGAGCGGGGGCGGAGCGCAG
CAGACGCCGGGCCTCGACAACGACACCGTGCGCATCCTCGACCAGGCCGAAACCATCGCCGACAAATCGGGCGACTCTTA
CGTCACCATCGAGCGGTTGCTCGTCGCGCTGGCGCTCGCCACCACCACATCGGCGGGGCAGGCGCTCAAGGCGGCCAATG
TCGATGCGAAGACCCTCGAAGCGGAAATCAGTGAGCTGCGCGGCGGGCGCACGGCGGACAGCGCCAACGCCGAAGAAGCC
TACGACGCGATGAAGAAATACGCCCGCGACCTCACCGAAGCTGCGCGCGAAGGCAAGCTCGACCCCGTGATCGGGCGCGA
CGAGGAGATCCGCAGGACGATCCAGATCCTTGCCCGCCGGACCAAGAACAACCCGGCGCTGATCGGCGATCCCGGCACCG
GCAAGACCGCCATCGCCGAAGGCCTCGCGCTGCGCATCGCCAATGGCGATGTGCCCGACAGCCTCAAGGGCCGTACGCTC
ATGTCGCTCGACATGGGCTCGCTCATCGCCGGCGCCAAATATCGCGGCGAATTCGAGGAGCGCCTGAAAGCCGTGCTCGA
CGAAGTGAAGGGCGCCGACGGGCAGATCATCCTTTTCATTGACGAGATGCACACGTTGATCGGGGCCGGCGCGAGCGAAG
GCTCGATGGATGCGTCCAACCTCTTGAAGCCCGCGCTCAGCCGCGGCGAACTGCACTGCATCGGCGCGACGACGCTCGAC
GAATATCAGAAATATGTCGAGAAGGACCCGGCGCTGCAGCGGCGCTTCCAGTCGGTCTATATCGACGAGCCGAGCGTCGA
GGACACGATCAGCATCCTGCGCGGCATTGCCGAGAAATATGAACTGCATCACGGCGTGAACATCACCGACGGCGCGCTGG
TGGCGGCGGCGCGGCTGTCCGATCGCTATATCCAGAACCGCTTCCTGCCCGACAAGGCGATCGACCTGATGGACGAGGCC
GCCTCGCGCATCCGCATGGAAGTGGAGAGCAAGCCCGAAGAGATCGAGAATCTCGATCGCCGCATAATCCAGCTCAAGAT
CGAAGAGCAGGCGCTCGAGAAAGAGACCGACAGCGCGTCCAAGGACCGCCTCGAAACCCTGCGCAAAGAATTGTCCGAAC
TCGAACAGAAATCGTCCGAACTGACCACGCGCTGGCAGAACGAGCGCGACAAGATCCAAGGCGAGACCAGGATCAAGGAA
GAACTCGACCAGGCGCGGATCGAACTCGAGCAGGCCGAGCGGTCGGGCGATCTCGCCAAGGCCGGCGAGCTGTCCTACGG
GCGAATCCCAGAGCTGGAAAAGAAGCTCGAGGAAGCGCGCGCGACCACGGAAAACGCGCTGCTCAAGGAAGAAGTGACCG
AAGACGATATCGCCAGCGTCGTTTCACGCTGGACCGGCATCCCCATCGACAAGATGCTCGAGGGCGAGCGCGACAAGCTG
CTCAAGATGGAAGAGCTGCTGTCGAAGCGCGTGATCGGCCAGGAAGAGCCCATTATCGCCGTGTCCAAGGCCGTCCGCCG
CGCGCGCGCGGGGTTGCAGGATCCGGGCCGCCCGCTCGGCAGTTTCCTGTTCCTCGGGCCGACCGGCGTCGGCAAGACCG
AACTGACCAAGGCGCTGGCCGGGTTCCTGTTCGATGATGACGACGCCATGGTCCGCATCGACATGTCCGAATTCATGGAG
AAACATTCGGTCGCCCGCCTGATCGGCGCACCTCCGGGCTATGTCGGCTATGACGAAGGCGGCGTGCTGACCGAGGCCGT
GCGGCGGCGGCCCTACCAGGTCGTGCTGTTCGACGAGGTCGAGAAGGCGCATACCGATGTTTTCAACGTGTTGCTGCAGG
TGCTCGACGATGGACGGCTGACGGACGGGCAGGGTCGGGTGGTGGATTTCAGCAACACGCTGATCATCCTGACCTCGAAT
CTCGGCAGCCAGTATCTCGCCAATATGGAGGATGGCCAGAAGGTCTCCGATGTCGAGCCGCAGGTGATGGACGTGGTGCG
CGGGCATTTCCGCCCCGAGTTTCTCAACCGGCTGGACGAGATCATCCTGTTCCACCGCCTGTCGATGGAGCACATGGCGC
CGATCGTCGATATCCAGGTGGGCCGCGTCCAGAAACTGCTGGCGGATCGCAAGATCGTGCTCGACCTGACCGATGCCGCC
AAGCGCTGGCTGGGGCGGGTCGGCTACGATCCGGTCTATGGCGCGCGCCCGCTCAAGCGCGCGGTGCAGCGCTACGTGCA
GGACCCACTCGCCGAACTGCTGCTGGAGGGCAAGGTGCCCGATGGAAGCACGGTCAAGATCGACGAGGGCGATGGCGAGT
TGGAAATGGAAGTCGCCTGA

Upstream 100 bases:

>100_bases
TCCTATATCCGACTAACACACCTCCTCGTCGAGGGGTTGAAGGACTTGTGTTGCACATTTACAACACTATATGTCTCGGG
TAACGATTGAGGGATAGGCG

Downstream 100 bases:

>100_bases
CGGCTACCCCAATACGGCTTTCGGTCAGGATATTCGGCCGACCATGCAATCCCGGCGGAGCGGGCGTGCCTGCCGCGCGC
AAGGGCTGTAATGATCTTTC

Product: ATP-dependent Clp protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MNLEKFTDRAKGFLQAAQTVAIRMSHQRITPAHLLKAMIEDPEGMASGLIARAGGNPRTVEDEVDAALGKIPAVSGGGAQ
QTPGLDNDTVRILDQAETIADKSGDSYVTIERLLVALALATTTSAGQALKAANVDAKTLEAEISELRGGRTADSANAEEA
YDAMKKYARDLTEAAREGKLDPVIGRDEEIRRTIQILARRTKNNPALIGDPGTGKTAIAEGLALRIANGDVPDSLKGRTL
MSLDMGSLIAGAKYRGEFEERLKAVLDEVKGADGQIILFIDEMHTLIGAGASEGSMDASNLLKPALSRGELHCIGATTLD
EYQKYVEKDPALQRRFQSVYIDEPSVEDTISILRGIAEKYELHHGVNITDGALVAAARLSDRYIQNRFLPDKAIDLMDEA
ASRIRMEVESKPEEIENLDRRIIQLKIEEQALEKETDSASKDRLETLRKELSELEQKSSELTTRWQNERDKIQGETRIKE
ELDQARIELEQAERSGDLAKAGELSYGRIPELEKKLEEARATTENALLKEEVTEDDIASVVSRWTGIPIDKMLEGERDKL
LKMEELLSKRVIGQEEPIIAVSKAVRRARAGLQDPGRPLGSFLFLGPTGVGKTELTKALAGFLFDDDDAMVRIDMSEFME
KHSVARLIGAPPGYVGYDEGGVLTEAVRRRPYQVVLFDEVEKAHTDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
LGSQYLANMEDGQKVSDVEPQVMDVVRGHFRPEFLNRLDEIILFHRLSMEHMAPIVDIQVGRVQKLLADRKIVLDLTDAA
KRWLGRVGYDPVYGARPLKRAVQRYVQDPLAELLLEGKVPDGSTVKIDEGDGELEMEVA

Sequences:

>Translated_859_residues
MNLEKFTDRAKGFLQAAQTVAIRMSHQRITPAHLLKAMIEDPEGMASGLIARAGGNPRTVEDEVDAALGKIPAVSGGGAQ
QTPGLDNDTVRILDQAETIADKSGDSYVTIERLLVALALATTTSAGQALKAANVDAKTLEAEISELRGGRTADSANAEEA
YDAMKKYARDLTEAAREGKLDPVIGRDEEIRRTIQILARRTKNNPALIGDPGTGKTAIAEGLALRIANGDVPDSLKGRTL
MSLDMGSLIAGAKYRGEFEERLKAVLDEVKGADGQIILFIDEMHTLIGAGASEGSMDASNLLKPALSRGELHCIGATTLD
EYQKYVEKDPALQRRFQSVYIDEPSVEDTISILRGIAEKYELHHGVNITDGALVAAARLSDRYIQNRFLPDKAIDLMDEA
ASRIRMEVESKPEEIENLDRRIIQLKIEEQALEKETDSASKDRLETLRKELSELEQKSSELTTRWQNERDKIQGETRIKE
ELDQARIELEQAERSGDLAKAGELSYGRIPELEKKLEEARATTENALLKEEVTEDDIASVVSRWTGIPIDKMLEGERDKL
LKMEELLSKRVIGQEEPIIAVSKAVRRARAGLQDPGRPLGSFLFLGPTGVGKTELTKALAGFLFDDDDAMVRIDMSEFME
KHSVARLIGAPPGYVGYDEGGVLTEAVRRRPYQVVLFDEVEKAHTDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
LGSQYLANMEDGQKVSDVEPQVMDVVRGHFRPEFLNRLDEIILFHRLSMEHMAPIVDIQVGRVQKLLADRKIVLDLTDAA
KRWLGRVGYDPVYGARPLKRAVQRYVQDPLAELLLEGKVPDGSTVKIDEGDGELEMEVA
>Mature_859_residues
MNLEKFTDRAKGFLQAAQTVAIRMSHQRITPAHLLKAMIEDPEGMASGLIARAGGNPRTVEDEVDAALGKIPAVSGGGAQ
QTPGLDNDTVRILDQAETIADKSGDSYVTIERLLVALALATTTSAGQALKAANVDAKTLEAEISELRGGRTADSANAEEA
YDAMKKYARDLTEAAREGKLDPVIGRDEEIRRTIQILARRTKNNPALIGDPGTGKTAIAEGLALRIANGDVPDSLKGRTL
MSLDMGSLIAGAKYRGEFEERLKAVLDEVKGADGQIILFIDEMHTLIGAGASEGSMDASNLLKPALSRGELHCIGATTLD
EYQKYVEKDPALQRRFQSVYIDEPSVEDTISILRGIAEKYELHHGVNITDGALVAAARLSDRYIQNRFLPDKAIDLMDEA
ASRIRMEVESKPEEIENLDRRIIQLKIEEQALEKETDSASKDRLETLRKELSELEQKSSELTTRWQNERDKIQGETRIKE
ELDQARIELEQAERSGDLAKAGELSYGRIPELEKKLEEARATTENALLKEEVTEDDIASVVSRWTGIPIDKMLEGERDKL
LKMEELLSKRVIGQEEPIIAVSKAVRRARAGLQDPGRPLGSFLFLGPTGVGKTELTKALAGFLFDDDDAMVRIDMSEFME
KHSVARLIGAPPGYVGYDEGGVLTEAVRRRPYQVVLFDEVEKAHTDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
LGSQYLANMEDGQKVSDVEPQVMDVVRGHFRPEFLNRLDEIILFHRLSMEHMAPIVDIQVGRVQKLLADRKIVLDLTDAA
KRWLGRVGYDPVYGARPLKRAVQRYVQDPLAELLLEGKVPDGSTVKIDEGDGELEMEVA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=370, Percent_Identity=35.4054054054054, Blast_Score=206, Evalue=1e-52,
Organism=Escherichia coli, GI1788943, Length=850, Percent_Identity=57.1764705882353, Blast_Score=989, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=455, Percent_Identity=39.1208791208791, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=706, Percent_Identity=55.8073654390935, Blast_Score=788, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=862, Percent_Identity=44.3155452436195, Blast_Score=698, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: 3.4.21.92

Molecular weight: Translated: 94760; Mature: 94760

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKFTDRAKGFLQAAQTVAIRMSHQRITPAHLLKAMIEDPEGMASGLIARAGGNPRTV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHEECCCCCCCC
EDEVDAALGKIPAVSGGGAQQTPGLDNDTVRILDQAETIADKSGDSYVTIERLLVALALA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
TTTSAGQALKAANVDAKTLEAEISELRGGRTADSANAEEAYDAMKKYARDLTEAAREGKL
HCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DPVIGRDEEIRRTIQILARRTKNNPALIGDPGTGKTAIAEGLALRIANGDVPDSLKGRTL
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEE
MSLDMGSLIAGAKYRGEFEERLKAVLDEVKGADGQIILFIDEMHTLIGAGASEGSMDASN
EEECHHHHHHCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCCCHHH
LLKPALSRGELHCIGATTLDEYQKYVEKDPALQRRFQSVYIDEPSVEDTISILRGIAEKY
HHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ELHHGVNITDGALVAAARLSDRYIQNRFLPDKAIDLMDEAASRIRMEVESKPEEIENLDR
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RIIQLKIEEQALEKETDSASKDRLETLRKELSELEQKSSELTTRWQNERDKIQGETRIKE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHH
ELDQARIELEQAERSGDLAKAGELSYGRIPELEKKLEEARATTENALLKEEVTEDDIASV
HHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VSRWTGIPIDKMLEGERDKLLKMEELLSKRVIGQEEPIIAVSKAVRRARAGLQDPGRPLG
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELTKALAGFLFDDDDAMVRIDMSEFMEKHSVARLIGAPPGYVGYDEG
CEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GVLTEAVRRRPYQVVLFDEVEKAHTDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
CHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECC
LGSQYLANMEDGQKVSDVEPQVMDVVRGHFRPEFLNRLDEIILFHRLSMEHMAPIVDIQV
CCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEHH
GRVQKLLADRKIVLDLTDAAKRWLGRVGYDPVYGARPLKRAVQRYVQDPLAELLLEGKVP
HHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DGSTVKIDEGDGELEMEVA
CCCEEEEECCCCCEEEEEC
>Mature Secondary Structure
MNLEKFTDRAKGFLQAAQTVAIRMSHQRITPAHLLKAMIEDPEGMASGLIARAGGNPRTV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHEECCCCCCCC
EDEVDAALGKIPAVSGGGAQQTPGLDNDTVRILDQAETIADKSGDSYVTIERLLVALALA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
TTTSAGQALKAANVDAKTLEAEISELRGGRTADSANAEEAYDAMKKYARDLTEAAREGKL
HCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DPVIGRDEEIRRTIQILARRTKNNPALIGDPGTGKTAIAEGLALRIANGDVPDSLKGRTL
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEE
MSLDMGSLIAGAKYRGEFEERLKAVLDEVKGADGQIILFIDEMHTLIGAGASEGSMDASN
EEECHHHHHHCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCCCHHH
LLKPALSRGELHCIGATTLDEYQKYVEKDPALQRRFQSVYIDEPSVEDTISILRGIAEKY
HHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ELHHGVNITDGALVAAARLSDRYIQNRFLPDKAIDLMDEAASRIRMEVESKPEEIENLDR
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RIIQLKIEEQALEKETDSASKDRLETLRKELSELEQKSSELTTRWQNERDKIQGETRIKE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHH
ELDQARIELEQAERSGDLAKAGELSYGRIPELEKKLEEARATTENALLKEEVTEDDIASV
HHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VSRWTGIPIDKMLEGERDKLLKMEELLSKRVIGQEEPIIAVSKAVRRARAGLQDPGRPLG
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELTKALAGFLFDDDDAMVRIDMSEFMEKHSVARLIGAPPGYVGYDEG
CEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GVLTEAVRRRPYQVVLFDEVEKAHTDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
CHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECC
LGSQYLANMEDGQKVSDVEPQVMDVVRGHFRPEFLNRLDEIILFHRLSMEHMAPIVDIQV
CCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEHH
GRVQKLLADRKIVLDLTDAAKRWLGRVGYDPVYGARPLKRAVQRYVQDPLAELLLEGKVP
HHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DGSTVKIDEGDGELEMEVA
CCCEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA