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The map label for this gene is amiD [H]

Identifier: 85373066

GI number: 85373066

Start: 275601

End: 276305

Strand: Direct

Name: amiD [H]

Synonym: ELI_01195

Alternate gene names: 85373066

Gene position: 275601-276305 (Clockwise)

Preceding gene: 85373065

Following gene: 85373067

Centisome position: 9.03

GC content: 62.98

Gene sequence:

>705_bases
ATGAGCGAACTGGTCCATCACGAGAGGCCCTCGCCCAATTTCAACGATCGCAAGCTGCCGATCAGCATGGTCGTCCTGCA
CTATACCGAGATGAAGCCGGTCGAGACCGCGCTCGACCGGCTATGCGATCCCGAGGGCGGGGTCAGTGCGCATTATCTCA
TCAGCGAGGAAGGCGAAGTCACCCGGCTGGTGCCGGAAGACAAGCGCGCCTGGCATGCGGGCGTCTCTTACTGGCGCGGG
CACAAGGATGTGAATTCGGCCAGCATCGGGATCGAGCTCGATCATCCCGGCCATGTGCTCGGTTATCGCGAATTCTCCGA
CGCGCAGATCGATGCGCTGATCCCGCTGCTCCACCGTATCGTGCAGAACCATGACATTCCGCGCGCCAATGTGGTCGGGC
ATTCGGACGTCGCGCCGGCGCGCAAGGTCGATCCGGGCGAGCTGTTTCCGTGGGACCGGCTGGCGGAGTACAATCTCTGC
CTGCCCAAGCCGGAGAAGCTGGACCTGGGCAATCCGTTCGACAATGCCGGTGCCTTCTACCTCGCGCTCGAACGGTTCGG
ATACGACGTTACCGACGGACTCAAGGCGGTCGAAGCCTTTCAGCGCCGCTGGCGCCCGGAGAAGATCGATGGCGAAATCG
ACGGCCAGATCTGCGCGATCCTGTTCCAGCTGTTGCTCGACCGCGACCGGGGCGTGTCCCGCTAA

Upstream 100 bases:

>100_bases
CCGCGCACATGCTGGCGCTAATCGCCGAGCAATCGGGCGGTAATCTGCAATACCAGAAATCGGAGGACGCTCTGGTCCTC
GGAGCGATGTTGCCCGAAGG

Downstream 100 bases:

>100_bases
CGGCGCGCACTGGCTTTCCTGCAAGGATAATCGCGCCTATATGTGCTCTTGCCAGGGGGCCGGGCAGCCGCTCCTTCGAC
AAGCTCAGGATGAGCGGGTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSELVHHERPSPNFNDRKLPISMVVLHYTEMKPVETALDRLCDPEGGVSAHYLISEEGEVTRLVPEDKRAWHAGVSYWRG
HKDVNSASIGIELDHPGHVLGYREFSDAQIDALIPLLHRIVQNHDIPRANVVGHSDVAPARKVDPGELFPWDRLAEYNLC
LPKPEKLDLGNPFDNAGAFYLALERFGYDVTDGLKAVEAFQRRWRPEKIDGEIDGQICAILFQLLLDRDRGVSR

Sequences:

>Translated_234_residues
MSELVHHERPSPNFNDRKLPISMVVLHYTEMKPVETALDRLCDPEGGVSAHYLISEEGEVTRLVPEDKRAWHAGVSYWRG
HKDVNSASIGIELDHPGHVLGYREFSDAQIDALIPLLHRIVQNHDIPRANVVGHSDVAPARKVDPGELFPWDRLAEYNLC
LPKPEKLDLGNPFDNAGAFYLALERFGYDVTDGLKAVEAFQRRWRPEKIDGEIDGQICAILFQLLLDRDRGVSR
>Mature_233_residues
SELVHHERPSPNFNDRKLPISMVVLHYTEMKPVETALDRLCDPEGGVSAHYLISEEGEVTRLVPEDKRAWHAGVSYWRGH
KDVNSASIGIELDHPGHVLGYREFSDAQIDALIPLLHRIVQNHDIPRANVVGHSDVAPARKVDPGELFPWDRLAEYNLCL
PKPEKLDLGNPFDNAGAFYLALERFGYDVTDGLKAVEAFQRRWRPEKIDGEIDGQICAILFQLLLDRDRGVSR

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=231, Percent_Identity=37.6623376623377, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1786300, Length=165, Percent_Identity=33.9393939393939, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26504; Mature: 26373

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELVHHERPSPNFNDRKLPISMVVLHYTEMKPVETALDRLCDPEGGVSAHYLISEEGEV
CCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCE
TRLVPEDKRAWHAGVSYWRGHKDVNSASIGIELDHPGHVLGYREFSDAQIDALIPLLHRI
EECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHH
VQNHDIPRANVVGHSDVAPARKVDPGELFPWDRLAEYNLCLPKPEKLDLGNPFDNAGAFY
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHE
LALERFGYDVTDGLKAVEAFQRRWRPEKIDGEIDGQICAILFQLLLDRDRGVSR
EEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SELVHHERPSPNFNDRKLPISMVVLHYTEMKPVETALDRLCDPEGGVSAHYLISEEGEV
CCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCE
TRLVPEDKRAWHAGVSYWRGHKDVNSASIGIELDHPGHVLGYREFSDAQIDALIPLLHRI
EECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHH
VQNHDIPRANVVGHSDVAPARKVDPGELFPWDRLAEYNLCLPKPEKLDLGNPFDNAGAFY
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHE
LALERFGYDVTDGLKAVEAFQRRWRPEKIDGEIDGQICAILFQLLLDRDRGVSR
EEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]