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The map label for this gene is tig

Identifier: 85373049

GI number: 85373049

Start: 256666

End: 258267

Strand: Direct

Name: tig

Synonym: ELI_01110

Alternate gene names: 85373049

Gene position: 256666-258267 (Clockwise)

Preceding gene: 85373045

Following gene: 85373054

Centisome position: 8.41

GC content: 60.67

Gene sequence:

>1602_bases
ATGCAAACCAAAGAGACCACGAATGAGGGCCTCAAGCGCGCCTACAAGCTGACGCTGACCGCCAAGGAAATCGATGCCAA
GATCGACGCCGAGGTGAAGAAAGTCGCCCCGCAAGTGCGGATGCCGGGTTTCCGCCCGGGCAAGGTGCCGGCCAATCTGG
TCCGCAAGATGCATGGCGAGCAAATGCACGCGCAGGTCATCAACGACTCGATCCGCGATTCGGTCGATGCGTTGATAAAG
GAAAAGGAACTGCGTCCCGCGATGCAGCCCAAGATCGACCTCAACGAGGATTACGAGCAGGGCAAGGACGCTGTCGTCTC
TGTCAGCCTCGAAATTCTGCCGAAGGTCGAAGCTCCGAGCATCGATGACCTGAAGTTGGAGCGGCTGACCGTTCCGGTGT
CGGATGAGCAGGTGATGGAAACCATCGAGCGCATTGCCGGCCAGAACAAGAGCTACAAGGATGCCGCCAAGACCAGGAAG
GCTGCTGACGGGGACCAGCTGATCATCGACTTCACCGGTTCGGTCGACGGTGTCGAGTTCGAAGGCGGCAAGGCCGAAGA
TGCCCCGCTGGTACTGGGATCGGGCACCTTCATCCCGGGCTTCGAAGAGCAGCTCAAGGGCGTGAAGACGGGCGACGAGA
AGACCATCACGGTCACTTTCCCCAAGGATTACCAGGCCGAGAACCTGGCTGGCAAGGAGGCCCAGTTCGACGTCAAGGTT
AAGCAGGTGAAGGTCGAGACCGACACCACGATCGATGACGAGTTTGCCGCCAACCTGGGCCTAGAAAACCTCGACAAGCT
GAAGGAATTGATCCGCGGCCAGCTGGAGCAGGAAACCAACGGCCTGACCCGTACCGCGATGAAGCGTTCGCTGCTCGACC
AGCTCGCCGCCGGGCACGATTTCCCGGTGCCGGAAGGCATGGTCGACGCTGAATTCGAGCAGATCTGGAACCAGCTGCAG
CAGGAAGCCGCGCAGGAAGAAGACCCGGACAAGGCGCTGAAGGAAATCGAAGCCGAGAAGGACGATTACCGCGCGATCGC
CGAACGCCGCGTACGGCTGGGCCTGCTGTTGTCGGAGATCGGCCAGGCCAATGGCGTCGAGATCAGCCAGCAGGAAATGT
CCATGCTGACGATGCAGGCGGCGCAGCAATATCGCGAGGAAGATCGCGAGCGCTTCATGCAATTCATCCAGCAGGATCCG
ATGGCCGCGGCCCAGCTGCGCGCGCCGCTGTATGAAGACAAGGTCGTCGACTTCCTGTTCGACAAGGCCGAGGTCACCGA
CCGCGAAGTGACGCGCGAGGAACTCGAAGCCGCGATCGAAGCGGAAGCCGAAGAAGAGAAGAAGCCGGCTGCGAAGAAGA
AGGCTCCGGCCAAGAAAGCCGAGCCCAAGAAGGCTGCTGCGAAAAAGGCCCCGGCCAAAAAGGCACCCGCGAAGAAAGCC
GCTGCCAAGGACGGCGACGAGAAGCCTGCCGCCAAGAAGGCGCCCGCCAAGAAAGCCCCGGCAAAGAAGGCTTCCACCAA
GAAGCCCGCCGAGAAGAAGGCTCCGGCAAAGAAGCCAGCCGCGAAAAAGGCTCCGGCCAAGAAGCCTGCTGCCAAAAAAT
AA

Upstream 100 bases:

>100_bases
ATCGAAAGATGTGGGGGTTCGAGTCCCTTCGCCCGCACCAGTCCGCCGCCGTAGGCCTCCACACAGAGCATTATTCGAAA
GAGCGCAAAGACCAAACGTT

Downstream 100 bases:

>100_bases
GCGCTCCTAGAGGGAAATTCAGCGCCGCCGAGCCCCGGGGTTCGGCGGCGTTCCCTATTGGTCGACCCGCCCTTCGACGG
CGATCCGCTTCTGGTCGGGC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MQTKETTNEGLKRAYKLTLTAKEIDAKIDAEVKKVAPQVRMPGFRPGKVPANLVRKMHGEQMHAQVINDSIRDSVDALIK
EKELRPAMQPKIDLNEDYEQGKDAVVSVSLEILPKVEAPSIDDLKLERLTVPVSDEQVMETIERIAGQNKSYKDAAKTRK
AADGDQLIIDFTGSVDGVEFEGGKAEDAPLVLGSGTFIPGFEEQLKGVKTGDEKTITVTFPKDYQAENLAGKEAQFDVKV
KQVKVETDTTIDDEFAANLGLENLDKLKELIRGQLEQETNGLTRTAMKRSLLDQLAAGHDFPVPEGMVDAEFEQIWNQLQ
QEAAQEEDPDKALKEIEAEKDDYRAIAERRVRLGLLLSEIGQANGVEISQQEMSMLTMQAAQQYREEDRERFMQFIQQDP
MAAAQLRAPLYEDKVVDFLFDKAEVTDREVTREELEAAIEAEAEEEKKPAAKKKAPAKKAEPKKAAAKKAPAKKAPAKKA
AAKDGDEKPAAKKAPAKKAPAKKASTKKPAEKKAPAKKPAAKKAPAKKPAAKK

Sequences:

>Translated_533_residues
MQTKETTNEGLKRAYKLTLTAKEIDAKIDAEVKKVAPQVRMPGFRPGKVPANLVRKMHGEQMHAQVINDSIRDSVDALIK
EKELRPAMQPKIDLNEDYEQGKDAVVSVSLEILPKVEAPSIDDLKLERLTVPVSDEQVMETIERIAGQNKSYKDAAKTRK
AADGDQLIIDFTGSVDGVEFEGGKAEDAPLVLGSGTFIPGFEEQLKGVKTGDEKTITVTFPKDYQAENLAGKEAQFDVKV
KQVKVETDTTIDDEFAANLGLENLDKLKELIRGQLEQETNGLTRTAMKRSLLDQLAAGHDFPVPEGMVDAEFEQIWNQLQ
QEAAQEEDPDKALKEIEAEKDDYRAIAERRVRLGLLLSEIGQANGVEISQQEMSMLTMQAAQQYREEDRERFMQFIQQDP
MAAAQLRAPLYEDKVVDFLFDKAEVTDREVTREELEAAIEAEAEEEKKPAAKKKAPAKKAEPKKAAAKKAPAKKAPAKKA
AAKDGDEKPAAKKAPAKKAPAKKASTKKPAEKKAPAKKPAAKKAPAKKPAAKK
>Mature_533_residues
MQTKETTNEGLKRAYKLTLTAKEIDAKIDAEVKKVAPQVRMPGFRPGKVPANLVRKMHGEQMHAQVINDSIRDSVDALIK
EKELRPAMQPKIDLNEDYEQGKDAVVSVSLEILPKVEAPSIDDLKLERLTVPVSDEQVMETIERIAGQNKSYKDAAKTRK
AADGDQLIIDFTGSVDGVEFEGGKAEDAPLVLGSGTFIPGFEEQLKGVKTGDEKTITVTFPKDYQAENLAGKEAQFDVKV
KQVKVETDTTIDDEFAANLGLENLDKLKELIRGQLEQETNGLTRTAMKRSLLDQLAAGHDFPVPEGMVDAEFEQIWNQLQ
QEAAQEEDPDKALKEIEAEKDDYRAIAERRVRLGLLLSEIGQANGVEISQQEMSMLTMQAAQQYREEDRERFMQFIQQDP
MAAAQLRAPLYEDKVVDFLFDKAEVTDREVTREELEAAIEAEAEEEKKPAAKKKAPAKKAEPKKAAAKKAPAKKAPAKKA
AAKDGDEKPAAKKAPAKKAPAKKASTKKPAEKKAPAKKPAAKKAPAKKPAAKK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=438, Percent_Identity=34.0182648401827, Blast_Score=228, Evalue=7e-61,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_ERYLH (Q2NDC7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457111.1
- ProteinModelPortal:   Q2NDC7
- STRING:   Q2NDC7
- GeneID:   3871606
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01110
- NMPDR:   fig|314225.3.peg.2868
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q2NDC7
- ProtClustDB:   PRK01490
- BioCyc:   ELIT314225:ELI_01110-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 58815; Mature: 58815

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKETTNEGLKRAYKLTLTAKEIDAKIDAEVKKVAPQVRMPGFRPGKVPANLVRKMHGE
CCCCCHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHH
QMHAQVINDSIRDSVDALIKEKELRPAMQPKIDLNEDYEQGKDAVVSVSLEILPKVEAPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCEEEEEEHHHCCCCCCCC
IDDLKLERLTVPVSDEQVMETIERIAGQNKSYKDAAKTRKAADGDQLIIDFTGSVDGVEF
CCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEE
EGGKAEDAPLVLGSGTFIPGFEEQLKGVKTGDEKTITVTFPKDYQAENLAGKEAQFDVKV
CCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEE
KQVKVETDTTIDDEFAANLGLENLDKLKELIRGQLEQETNGLTRTAMKRSLLDQLAAGHD
EEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
FPVPEGMVDAEFEQIWNQLQQEAAQEEDPDKALKEIEAEKDDYRAIAERRVRLGLLLSEI
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GQANGVEISQQEMSMLTMQAAQQYREEDRERFMQFIQQDPMAAAQLRAPLYEDKVVDFLF
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHH
DKAEVTDREVTREELEAAIEAEAEEEKKPAAKKKAPAKKAEPKKAAAKKAPAKKAPAKKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCCCCCCCCHHHH
AAKDGDEKPAAKKAPAKKAPAKKASTKKPAEKKAPAKKPAAKKAPAKKPAAKK
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQTKETTNEGLKRAYKLTLTAKEIDAKIDAEVKKVAPQVRMPGFRPGKVPANLVRKMHGE
CCCCCHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHH
QMHAQVINDSIRDSVDALIKEKELRPAMQPKIDLNEDYEQGKDAVVSVSLEILPKVEAPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCEEEEEEHHHCCCCCCCC
IDDLKLERLTVPVSDEQVMETIERIAGQNKSYKDAAKTRKAADGDQLIIDFTGSVDGVEF
CCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEE
EGGKAEDAPLVLGSGTFIPGFEEQLKGVKTGDEKTITVTFPKDYQAENLAGKEAQFDVKV
CCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEE
KQVKVETDTTIDDEFAANLGLENLDKLKELIRGQLEQETNGLTRTAMKRSLLDQLAAGHD
EEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
FPVPEGMVDAEFEQIWNQLQQEAAQEEDPDKALKEIEAEKDDYRAIAERRVRLGLLLSEI
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GQANGVEISQQEMSMLTMQAAQQYREEDRERFMQFIQQDPMAAAQLRAPLYEDKVVDFLF
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHH
DKAEVTDREVTREELEAAIEAEAEEEKKPAAKKKAPAKKAEPKKAAAKKAPAKKAPAKKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCCCCCCCCHHHH
AAKDGDEKPAAKKAPAKKAPAKKASTKKPAEKKAPAKKPAAKKAPAKKPAAKK
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA