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The map label for this gene is murE [H]

Identifier: 84625265

GI number: 84625265

Start: 4082880

End: 4084367

Strand: Reverse

Name: murE [H]

Synonym: XOO_3608

Alternate gene names: 84625265

Gene position: 4084367-4082880 (Counterclockwise)

Preceding gene: 84625266

Following gene: 84625264

Centisome position: 82.68

GC content: 66.4

Gene sequence:

>1488_bases
GTGAGCCGTTCCATGGCGCTTTCGCAGTTGCTGCCGGACGTGGCGCTGCCGCACGACGTGCAGGTCTCCGGTCTGGTGAT
GGATAGCCGTACCGTTGCTCCGGGCGATGCGTTCGTGGCGATCGCCGGCTTCGGTGCGCATGGCCTGGGCTTTGTCGAAC
AAGCGCGCGCCAATGGCGCAACAGCGGTGTTGTTCGAGCCGCCGGCTCCGGACGGTCTGCCGGTCCCCGTCGATGCGATT
GCCGTGCCCGGTTTGCGCGCGCGCCTTGGCGTGATGGCCGATCAATTTCATGGCCGGCCGTCGCATGCAATGCGCATGGT
CGGCGTCACTGGCACCAATGGCAAGACCTCCACCGTACAGTTGCTGGCGCAGGCGCTGACGCTGCTCGGTACGCCCACCG
GCACGCTCGGCACCCTTGGCGTCGGTCTGTATGGTGCGGCCGTGTCGACCGGTTTCACCACGCCGCTGGTGCTGCCCACG
CATGCGTTGCTGGCGCAGTTGCGCGATGAGGGCGCGCAGGCGGTGGCAATGGAGGTCAGCTCGCACGCGCTCGATCAGGG
CCGCGTGGATGCGGTGCATTTCGACGTCGCGGTGTTCACCAATCTCACCCGCGATCATCTCGATTACCACGGCGAGATGG
CGCAATACGGCGCCGCGAAGGCCAAGCTGTTCACCCGCCCTGGCTTGAAAGCCGCCGTGGTGAATCTGGACGATGGCTTC
GGCCGCACGCTGTTCGCTTCGCGCGATCCCGTGCTGCGCGCGATCGGCGTGAGCTCGCGCGCACATGCCGATGCCAGCGT
GAGCGCGCAGGCATTGCAGCTGGACCACAACGGCATCAACTTCGCCCTGAATATCCAGGGCGAAGTGCATCCGGTGCATT
CGCCGTTGCTGGGCCGCTTCAACGTGGACAACCTGCTGGCCGTGGCCGGCGCGTTGTGGGCGCTGGACATCGCACCCGCG
CAGATCGCCACCGTGCTCGGTCAGTTGCAGCCGATCCATGGCCGCATGAACCGTCTCGGTGGCGCACATGGCGCACCATT
GGTGGTGGTCGATTACGCACACACCCCGGATGCGCTGGAACAGGCATTGAGCAGCCTGCGTTCGCATGCGCAGGACCGGC
TGATCTGCGTGTTCGGCTGCGGTGGTGAACGTGACACCGGCAAACGCCCGCAGATGGCGGCGATTGCAGAACTCAACGCC
GATGTGGCAATCGTCACCGACGACAACCCGCGTGGCGAAGATGGCGATGCGATTGTGGCCGATATCCTGCGCGGCTTCGC
GCGCCCGGATGCCGCCATCGTACAGCGCGACCGCGCCGCAGCGATCCATCAGGCCATCGCCATGGCCAGCGCGTCCGACA
TCGTGTTGATCGCCGGCAAGGGACACGAGCCATACCAAGAAGTTGCCGGCGTGCGGCATGCCTTCGACGACGCCATCGTG
GCGGCGCAGGCGCTGCTGCCGCGCACCGTGTTGGGAGTGCGCCCATGA

Upstream 100 bases:

>100_bases
CGGACCCGGTGGACGAAGTGTCTGCCGGCATCCCGACCGCACTGGCGCCTGCGCCGGCCGCCGCGCCAGCGCAGCCCGCG
CCGTTGCAGGAGAGCCGCCA

Downstream 100 bases:

>100_bases
AGCTCACCCCACTGTCATTGATTGCGCATTGGGCCGGCGGCGAACTGCATGGCGACGACGCCATGATCGATGCGGTCGGC
AACGACACCCGCACGCTCGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSRSMALSQLLPDVALPHDVQVSGLVMDSRTVAPGDAFVAIAGFGAHGLGFVEQARANGATAVLFEPPAPDGLPVPVDAI
AVPGLRARLGVMADQFHGRPSHAMRMVGVTGTNGKTSTVQLLAQALTLLGTPTGTLGTLGVGLYGAAVSTGFTTPLVLPT
HALLAQLRDEGAQAVAMEVSSHALDQGRVDAVHFDVAVFTNLTRDHLDYHGEMAQYGAAKAKLFTRPGLKAAVVNLDDGF
GRTLFASRDPVLRAIGVSSRAHADASVSAQALQLDHNGINFALNIQGEVHPVHSPLLGRFNVDNLLAVAGALWALDIAPA
QIATVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALEQALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELNA
DVAIVTDDNPRGEDGDAIVADILRGFARPDAAIVQRDRAAAIHQAIAMASASDIVLIAGKGHEPYQEVAGVRHAFDDAIV
AAQALLPRTVLGVRP

Sequences:

>Translated_495_residues
MSRSMALSQLLPDVALPHDVQVSGLVMDSRTVAPGDAFVAIAGFGAHGLGFVEQARANGATAVLFEPPAPDGLPVPVDAI
AVPGLRARLGVMADQFHGRPSHAMRMVGVTGTNGKTSTVQLLAQALTLLGTPTGTLGTLGVGLYGAAVSTGFTTPLVLPT
HALLAQLRDEGAQAVAMEVSSHALDQGRVDAVHFDVAVFTNLTRDHLDYHGEMAQYGAAKAKLFTRPGLKAAVVNLDDGF
GRTLFASRDPVLRAIGVSSRAHADASVSAQALQLDHNGINFALNIQGEVHPVHSPLLGRFNVDNLLAVAGALWALDIAPA
QIATVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALEQALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELNA
DVAIVTDDNPRGEDGDAIVADILRGFARPDAAIVQRDRAAAIHQAIAMASASDIVLIAGKGHEPYQEVAGVRHAFDDAIV
AAQALLPRTVLGVRP
>Mature_494_residues
SRSMALSQLLPDVALPHDVQVSGLVMDSRTVAPGDAFVAIAGFGAHGLGFVEQARANGATAVLFEPPAPDGLPVPVDAIA
VPGLRARLGVMADQFHGRPSHAMRMVGVTGTNGKTSTVQLLAQALTLLGTPTGTLGTLGVGLYGAAVSTGFTTPLVLPTH
ALLAQLRDEGAQAVAMEVSSHALDQGRVDAVHFDVAVFTNLTRDHLDYHGEMAQYGAAKAKLFTRPGLKAAVVNLDDGFG
RTLFASRDPVLRAIGVSSRAHADASVSAQALQLDHNGINFALNIQGEVHPVHSPLLGRFNVDNLLAVAGALWALDIAPAQ
IATVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALEQALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELNAD
VAIVTDDNPRGEDGDAIVADILRGFARPDAAIVQRDRAAAIHQAIAMASASDIVLIAGKGHEPYQEVAGVRHAFDDAIVA
AQALLPRTVLGVRP

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=473, Percent_Identity=44.6088794926004, Blast_Score=306, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51494; Mature: 51363

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSMALSQLLPDVALPHDVQVSGLVMDSRTVAPGDAFVAIAGFGAHGLGFVEQARANGA
CCCHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCCCCC
TAVLFEPPAPDGLPVPVDAIAVPGLRARLGVMADQFHGRPSHAMRMVGVTGTNGKTSTVQ
EEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHH
LLAQALTLLGTPTGTLGTLGVGLYGAAVSTGFTTPLVLPTHALLAQLRDEGAQAVAMEVS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHH
SHALDQGRVDAVHFDVAVFTNLTRDHLDYHGEMAQYGAAKAKLFTRPGLKAAVVNLDDGF
HHHCCCCCEEEEEEEHHHHHHHHHHHHHHCCHHHHHCCHHHEEEECCCCEEEEEECCCCC
GRTLFASRDPVLRAIGVSSRAHADASVSAQALQLDHNGINFALNIQGEVHPVHSPLLGRF
CCEEECCCCCHHEEECCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCEEECCCCCCCCC
NVDNLLAVAGALWALDIAPAQIATVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALE
CHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHH
QALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELNADVAIVTDDNPRGEDGDAIVA
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCCCCHHHH
DILRGFARPDAAIVQRDRAAAIHQAIAMASASDIVLIAGKGHEPYQEVAGVRHAFDDAIV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
AAQALLPRTVLGVRP
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRSMALSQLLPDVALPHDVQVSGLVMDSRTVAPGDAFVAIAGFGAHGLGFVEQARANGA
CCHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCCCCC
TAVLFEPPAPDGLPVPVDAIAVPGLRARLGVMADQFHGRPSHAMRMVGVTGTNGKTSTVQ
EEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHH
LLAQALTLLGTPTGTLGTLGVGLYGAAVSTGFTTPLVLPTHALLAQLRDEGAQAVAMEVS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHH
SHALDQGRVDAVHFDVAVFTNLTRDHLDYHGEMAQYGAAKAKLFTRPGLKAAVVNLDDGF
HHHCCCCCEEEEEEEHHHHHHHHHHHHHHCCHHHHHCCHHHEEEECCCCEEEEEECCCCC
GRTLFASRDPVLRAIGVSSRAHADASVSAQALQLDHNGINFALNIQGEVHPVHSPLLGRF
CCEEECCCCCHHEEECCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCEEECCCCCCCCC
NVDNLLAVAGALWALDIAPAQIATVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALE
CHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHH
QALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELNADVAIVTDDNPRGEDGDAIVA
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCCCCHHHH
DILRGFARPDAAIVQRDRAAAIHQAIAMASASDIVLIAGKGHEPYQEVAGVRHAFDDAIV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
AAQALLPRTVLGVRP
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]