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The map label for this gene is nudG [C]

Identifier: 84625250

GI number: 84625250

Start: 4063955

End: 4064902

Strand: Reverse

Name: nudG [C]

Synonym: XOO_3593

Alternate gene names: 84625250

Gene position: 4064902-4063955 (Counterclockwise)

Preceding gene: 84625251

Following gene: 84625246

Centisome position: 82.28

GC content: 66.24

Gene sequence:

>948_bases
ATGCCTGATTCTCTTCGATCCATTCACGTCGTTGCCGGCGTGATCACCGACCCCCGCGGGCGCATCTTGCTGACCCGTCG
TACAGAAACCCGCGACATGCCTGGCCTGTGGGAATTCCCGGGTGGCAAGCGTGAGCCCGGCGAAACGTCCGAACAAGCGC
TGGTGCGCGAGCTCAACGAAGAGCTTGGCATCGAGGCGCAGGTCGGGGACTGGCTCATGGATGTGCCGCAGCTGTATCCG
GACAAGCGGCTGCGTCTGGAAGTGCGTCACATCACCGCCTGGAAAGGCAGCCCACGTGGGCGCGAAGGCCAGGCCATGAC
CTGGGTGGCGGCAGACAAGCTGGCGCGCTATTCGATGCCGCCGGCCGATGTGCCGGTGGTGGGTGCGCTGCGCCAGCCCG
ACCATTACCTGATCACCCCCGAACCGGAGGACGACGCACGCTGGCTGGAAGGCCTGGAGCGCGCATTGCAGAATGGGATC
ACGCGGATCCAGCTGCGCGCGCGACAGACAGCACCTGCGCAGTGGCAGGCGCTGCTGCAGCAGGTCATGCGCTTACGTGG
ACGCACCCGCGCGCAGCTGCTGCTCAATCGCGATATCGCGCTGGCTGCGGAACTGGGGGTCGGCGTGCATCTGGGGTCGG
AGCAGTTGGCCGGTCTGCAGGAACGCCCGCTGCCCGCAGAACAGCTGGTGGCTGCTTCCTGTCATGGCTTGGACGACTTG
CGCCACGCGCAACGGATCGGCTGCGACTTCGCGGTGTTGGGGCCGGTGCAGGCCACCGCATCGCACCCGGGCGCCACACC
TCTCGGATGGGGCGGTTTCGAAACCTTGCGTGAACAGGTCTCGCTGCCGATCTACGCGCTTGGCGGCATGCAGATTGAAG
ATGTGCGCGAGGCGCGCTCGCATGGTGCGCAGGGCATTGCGGCGATCCGCTCGCTTTGGCCGCAATAA

Upstream 100 bases:

>100_bases
CCTGTAGTGCACCAGCTGTTACCAATCCCTACTCACGAATCACGAATCCCAGCCCTCAAGCGATTGCGCTGAACCGCTCA
CTCGCGGCACTCTTGCGTCC

Downstream 100 bases:

>100_bases
CCGCACCGACGCTGGTGGTGTTCGGCGGTTTGCCGGGCGTTGGTAAGCGTTCAATCGCTTCGTTATTAGGGCCGTGCGTA
CAACGCAGCGAGCAGCGGTC

Product: hypothetical protein

Products: CMP; diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPDSLRSIHVVAGVITDPRGRILLTRRTETRDMPGLWEFPGGKREPGETSEQALVRELNEELGIEAQVGDWLMDVPQLYP
DKRLRLEVRHITAWKGSPRGREGQAMTWVAADKLARYSMPPADVPVVGALRQPDHYLITPEPEDDARWLEGLERALQNGI
TRIQLRARQTAPAQWQALLQQVMRLRGRTRAQLLLNRDIALAAELGVGVHLGSEQLAGLQERPLPAEQLVAASCHGLDDL
RHAQRIGCDFAVLGPVQATASHPGATPLGWGGFETLREQVSLPIYALGGMQIEDVREARSHGAQGIAAIRSLWPQ

Sequences:

>Translated_315_residues
MPDSLRSIHVVAGVITDPRGRILLTRRTETRDMPGLWEFPGGKREPGETSEQALVRELNEELGIEAQVGDWLMDVPQLYP
DKRLRLEVRHITAWKGSPRGREGQAMTWVAADKLARYSMPPADVPVVGALRQPDHYLITPEPEDDARWLEGLERALQNGI
TRIQLRARQTAPAQWQALLQQVMRLRGRTRAQLLLNRDIALAAELGVGVHLGSEQLAGLQERPLPAEQLVAASCHGLDDL
RHAQRIGCDFAVLGPVQATASHPGATPLGWGGFETLREQVSLPIYALGGMQIEDVREARSHGAQGIAAIRSLWPQ
>Mature_314_residues
PDSLRSIHVVAGVITDPRGRILLTRRTETRDMPGLWEFPGGKREPGETSEQALVRELNEELGIEAQVGDWLMDVPQLYPD
KRLRLEVRHITAWKGSPRGREGQAMTWVAADKLARYSMPPADVPVVGALRQPDHYLITPEPEDDARWLEGLERALQNGIT
RIQLRARQTAPAQWQALLQQVMRLRGRTRAQLLLNRDIALAAELGVGVHLGSEQLAGLQERPLPAEQLVAASCHGLDDLR
HAQRIGCDFAVLGPVQATASHPGATPLGWGGFETLREQVSLPIYALGGMQIEDVREARSHGAQGIAAIRSLWPQ

Specific function: Specific For Pyrimidine Substrates. Acts On 5-Methyl- Dctp, Ctp And Dctp In Decreasing Order. [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=126, Percent_Identity=40.4761904761905, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786288, Length=128, Percent_Identity=36.71875, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 34826; Mature: 34695

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSLRSIHVVAGVITDPRGRILLTRRTETRDMPGLWEFPGGKREPGETSEQALVRELNE
CCCCHHEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ELGIEAQVGDWLMDVPQLYPDKRLRLEVRHITAWKGSPRGREGQAMTWVAADKLARYSMP
HHCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHCCCC
PADVPVVGALRQPDHYLITPEPEDDARWLEGLERALQNGITRIQLRARQTAPAQWQALLQ
CCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
QVMRLRGRTRAQLLLNRDIALAAELGVGVHLGSEQLAGLQERPLPAEQLVAASCHGLDDL
HHHHHHCCCHHHHHHCCCHHEEHHCCCCEEECHHHHCCCCCCCCCHHHHHHHHHCCHHHH
RHAQRIGCDFAVLGPVQATASHPGATPLGWGGFETLREQVSLPIYALGGMQIEDVREARS
HHHHHHCCCEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHH
HGAQGIAAIRSLWPQ
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
PDSLRSIHVVAGVITDPRGRILLTRRTETRDMPGLWEFPGGKREPGETSEQALVRELNE
CCCHHEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ELGIEAQVGDWLMDVPQLYPDKRLRLEVRHITAWKGSPRGREGQAMTWVAADKLARYSMP
HHCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHCCCC
PADVPVVGALRQPDHYLITPEPEDDARWLEGLERALQNGITRIQLRARQTAPAQWQALLQ
CCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
QVMRLRGRTRAQLLLNRDIALAAELGVGVHLGSEQLAGLQERPLPAEQLVAASCHGLDDL
HHHHHHCCCHHHHHHCCCHHEEHHCCCCEEECHHHHCCCCCCCCCHHHHHHHHHCCHHHH
RHAQRIGCDFAVLGPVQATASHPGATPLGWGGFETLREQVSLPIYALGGMQIEDVREARS
HHHHHHCCCEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHH
HGAQGIAAIRSLWPQ
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]