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The map label for this gene is leuB [H]

Identifier: 84622583

GI number: 84622583

Start: 1010163

End: 1011170

Strand: Direct

Name: leuB [H]

Synonym: XOO_0926

Alternate gene names: 84622583

Gene position: 1010163-1011170 (Clockwise)

Preceding gene: 84622581

Following gene: 84622585

Centisome position: 20.45

GC content: 65.38

Gene sequence:

>1008_bases
ATGACGCAGACAATCACGGTCATCCGCGGCGACGGTATCGGCCCGGAGATCATGGATGCCACGCTGTTCGTGCTCGACGC
GCTGCAAGCTGGCCTGACCTACGAATACGCAGACGCCGGCCTGGTCGCGCTGGAAAAGCACGGCGATCTGCTGCCCGAGT
CGACCCTGGCCTCGATCAGCAAGAACAAGGTGGCGCTGAAAAGCCCGTTGACCACGCCGGTCGGCGAGGGCTTCAGCTCG
ATCAACGTCGCCATGCGTCGCAAGTTCGACCTGTACGCGAACGTGCGTCCGGCCAAGTCGTTCCCGAACACCAAGTCGCG
TTTCGCCGATGGCGTGGACCTGATCACGGTGCGCGAAAACACCGAAGGTGCCTACCTGAGCGAAGGCCAGACCGTGTCCG
AGGATGGCGAGACCGCGTTCTCCGGCATCCTGATCACTCGCAAGGGCTCCGAGCGCATCGTGCGCTACGCCTTCGACCTG
GCGCGCGCCACCGGCCGCAAGAAGGTCACCGCGGTGCACAAGGCCAACATCATCAAGTCGACCTCGGGCCTGTTCCTGAA
GGTCGCCCGCGATGTGGCCGCGCAGTACCCGGAAATCGAATTCCAGGAAATGATCGTCGACAACACCTGCATGCAGCTGG
TGATGCGTCCGGAGCAGTTCGACATCATCGTCACCACCAACCTGTTCGGCGACATCCTCTCCGACCTGTGCGCCGGTCTG
GTCGGCGGCCTGGGCCTGGCCCCGGGCGCCAACATCGGTCTGGACGCGGCGATCTTCGAAGCCGTGCACGGCTCGGCGCC
GGATATCGCAGGGCAGGGCAAGGCCAATCCGTGCGCGCTGCTGCTGGGCGCCGCGCAGATGCTGGACCACATCGGCCAGC
CGCAGAACGCCGAGCGCCTGCGCGAAGCCATCGTCGCCACGCTGGAAGCCAAGGATTCCTTGACCCCGGACCTGGGCGGC
AGCGGCAACACCATGGGCTTCGCCAAGGCCATCGCCAGCCGCCTCTGA

Upstream 100 bases:

>100_bases
AGTCCCGGCATCGGGCATAATTGCCGGTGAGACCTCATTGCGCCGATCTGCCGGGTTTGACGGCTGGCGCTCCCCCCTTC
AAGTTCGGAAACCCAACGTT

Downstream 100 bases:

>100_bases
GTGCACTGGTTGATCGCACAATAAAGATAAGGGCGCCTAGGCGCCCTTTTGCTTTTGCTTGGGATTCGTCTTGAGCCTTT
CTCGGCCATCAATCGCAGGG

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTQTITVIRGDGIGPEIMDATLFVLDALQAGLTYEYADAGLVALEKHGDLLPESTLASISKNKVALKSPLTTPVGEGFSS
INVAMRRKFDLYANVRPAKSFPNTKSRFADGVDLITVRENTEGAYLSEGQTVSEDGETAFSGILITRKGSERIVRYAFDL
ARATGRKKVTAVHKANIIKSTSGLFLKVARDVAAQYPEIEFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
VGGLGLAPGANIGLDAAIFEAVHGSAPDIAGQGKANPCALLLGAAQMLDHIGQPQNAERLREAIVATLEAKDSLTPDLGG
SGNTMGFAKAIASRL

Sequences:

>Translated_335_residues
MTQTITVIRGDGIGPEIMDATLFVLDALQAGLTYEYADAGLVALEKHGDLLPESTLASISKNKVALKSPLTTPVGEGFSS
INVAMRRKFDLYANVRPAKSFPNTKSRFADGVDLITVRENTEGAYLSEGQTVSEDGETAFSGILITRKGSERIVRYAFDL
ARATGRKKVTAVHKANIIKSTSGLFLKVARDVAAQYPEIEFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
VGGLGLAPGANIGLDAAIFEAVHGSAPDIAGQGKANPCALLLGAAQMLDHIGQPQNAERLREAIVATLEAKDSLTPDLGG
SGNTMGFAKAIASRL
>Mature_334_residues
TQTITVIRGDGIGPEIMDATLFVLDALQAGLTYEYADAGLVALEKHGDLLPESTLASISKNKVALKSPLTTPVGEGFSSI
NVAMRRKFDLYANVRPAKSFPNTKSRFADGVDLITVRENTEGAYLSEGQTVSEDGETAFSGILITRKGSERIVRYAFDLA
RATGRKKVTAVHKANIIKSTSGLFLKVARDVAAQYPEIEFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGLV
GGLGLAPGANIGLDAAIFEAVHGSAPDIAGQGKANPCALLLGAAQMLDHIGQPQNAERLREAIVATLEAKDSLTPDLGGS
GNTMGFAKAIASRL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=334, Percent_Identity=47.3053892215569, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI4758582, Length=326, Percent_Identity=45.0920245398773, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI28178838, Length=318, Percent_Identity=44.3396226415094, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI28178821, Length=341, Percent_Identity=39.8826979472141, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI28178816, Length=341, Percent_Identity=39.5894428152493, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=44.3298969072165, Blast_Score=167, Evalue=9e-42,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=36.056338028169, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787381, Length=359, Percent_Identity=35.3760445682451, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI87081683, Length=307, Percent_Identity=37.1335504885993, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71986051, Length=333, Percent_Identity=45.045045045045, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI25144293, Length=334, Percent_Identity=41.6167664670659, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI17550882, Length=339, Percent_Identity=41.2979351032448, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17505779, Length=326, Percent_Identity=39.2638036809816, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324709, Length=336, Percent_Identity=43.1547619047619, Blast_Score=275, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6324291, Length=338, Percent_Identity=40.2366863905325, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=38.0681818181818, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=29.1666666666667, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643268, Length=335, Percent_Identity=45.9701492537313, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24643270, Length=335, Percent_Identity=45.9701492537313, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=41.5697674418605, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI161078633, Length=333, Percent_Identity=39.039039039039, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24650122, Length=333, Percent_Identity=39.039039039039, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI161078637, Length=333, Percent_Identity=39.039039039039, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI161078635, Length=333, Percent_Identity=39.039039039039, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI161078639, Length=326, Percent_Identity=39.5705521472393, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI281362242, Length=335, Percent_Identity=41.1940298507463, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24648872, Length=335, Percent_Identity=41.1940298507463, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI20130355, Length=340, Percent_Identity=28.8235294117647, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI45550436, Length=233, Percent_Identity=24.8927038626609, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35529; Mature: 35398

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTITVIRGDGIGPEIMDATLFVLDALQAGLTYEYADAGLVALEKHGDLLPESTLASIS
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCHHHHHHHH
KNKVALKSPLTTPVGEGFSSINVAMRRKFDLYANVRPAKSFPNTKSRFADGVDLITVREN
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHCCCCEEEEEEEC
TEGAYLSEGQTVSEDGETAFSGILITRKGSERIVRYAFDLARATGRKKVTAVHKANIIKS
CCCCEECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TSGLFLKVARDVAAQYPEIEFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
VGGLGLAPGANIGLDAAIFEAVHGSAPDIAGQGKANPCALLLGAAQMLDHIGQPQNAERL
HCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
REAIVATLEAKDSLTPDLGGSGNTMGFAKAIASRL
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TQTITVIRGDGIGPEIMDATLFVLDALQAGLTYEYADAGLVALEKHGDLLPESTLASIS
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCHHHHHHHH
KNKVALKSPLTTPVGEGFSSINVAMRRKFDLYANVRPAKSFPNTKSRFADGVDLITVREN
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHCCCCEEEEEEEC
TEGAYLSEGQTVSEDGETAFSGILITRKGSERIVRYAFDLARATGRKKVTAVHKANIIKS
CCCCEECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TSGLFLKVARDVAAQYPEIEFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
VGGLGLAPGANIGLDAAIFEAVHGSAPDIAGQGKANPCALLLGAAQMLDHIGQPQNAERL
HCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
REAIVATLEAKDSLTPDLGGSGNTMGFAKAIASRL
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]