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The map label for this gene is purA [H]

Identifier: 84622497

GI number: 84622497

Start: 914115

End: 915407

Strand: Direct

Name: purA [H]

Synonym: XOO_0840

Alternate gene names: 84622497

Gene position: 914115-915407 (Clockwise)

Preceding gene: 84622496

Following gene: 84622498

Centisome position: 18.5

GC content: 65.43

Gene sequence:

>1293_bases
ATGGGTCAGTCAGTTGTCGTGCTCGGTGCCCAGTGGGGCGATGAAGGCAAGGGCAAGATCGTCGATCTGCTTACCGAAGA
AATCGGTGCGGTCGTCCGCTTCCAGGGCGGCCATAACGCCGGCCATACGCTCGTCATCAATGGCAAGAAGACCGTCTTGC
ATCTGATCCCGTCCGGCATCCTGCGCGACGACGCGCTGTGCCTGATCGGCAATGGCGTGGTGATCTCCCCGGCCGCGTTG
ATCAAAGAGGTCAGCGAGCTGGAAGACGCCGGCGTGGAAGTGCGCTCGCGCCTGAAGATTTCCCCTGCCGCGCCGTTGAT
CATGCCGTACCACATCGCCCTGGATCAGGCGCGCGAAAAAGCGGCCGGCGGCAAGGCCATCGGCACCACCGGCCGTGGTA
TCGGCCCGGCCTATGAAGACAAGGTGGCGCGTCGCGGCATCCGCATCGCCGACCTGCATTACCCGCCGCAGCTGGAAGAG
CTGCTGCGCACCGCGCTGGATTACCACAACTTCGTGCTGACCAAGTACCTGGGCGTGGAAGCGGTCGATTTCCAGAAGAC
CTACGACGAAGCGCTGGCCTTCGGCGACTACGTCCAGCCGATGAAGTCGGACGTGGCCGGCATCCTGCACGACCTGCGCA
AGCAGGGCAAGCGCGTGCTGTTCGAAGGCGCGCAGGGCGCGTTGCTGGACATCGATCACGGCACCTATCCCTACGTCACC
AGCTCCAATACCACTGTGGGCGGCGCGCTGGCCGGCACCGGTGTGGGCGCCGATGCGATCGACTACGTGTTGGGTATCGC
CAAGGCGTACGCCACGCGCGTCGGCGGCGGCCCGTTCCCGACCGAGCTGGACGATGCAGTGGGCCAGGGCATCCGCGACC
GCGGTGCCGAGTACGGTGCCTCCACCGGCCGCCCGCGCCGCTGCGGCTGGATGGACATCGTTGCGCTCAAGCGTGCGGTG
GCCATCAACGGTATCTCCGGCCTGTGCATCACCAAGCTCGACGTGCTCGATGGCATGGAAAAGCTCAAGGTCTGCATCGC
CTACGAATACCGCGGCAAGCGCACCGAATATGCACCGCTGGACGCGCAGGGTTGGGAAGAGTGCACCCCTGTGTACCTGG
AATTCCCGGGCTGGACCGAGAACACCCATGGCATCACCGAGTGGGACAAGCTGCCCGTCGCGGCCCGCGCCTACCTGCGC
GCGTTGGAAGAACTGGCTGGCTGCCCGATCTCGATCGTCTCCACCGGCCCGGACCGCGACCACACCATGGTGCTGCAGGA
CCCGTTCGCGTGA

Upstream 100 bases:

>100_bases
GCTCCCCGATGGAGCTGCGCGCAGGGTGTCGCTGCCAACCCTGCGTCGGTCCCATCCCGCGGCCCCGATGGCCGCAGCAA
AACTCAGGAGTTCACCCGTC

Downstream 100 bases:

>100_bases
TCGGCTGATGCGTGAGTGAAGCGACAAAAGCCCTGCGAAAGCGGGGCTTTTGTGTGCGTGATGCTGTCGAGCGCTGCTTT
TGCAAGCCCTATCGGTGCGG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGILRDDALCLIGNGVVISPAAL
IKEVSELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREKAAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEE
LLRTALDYHNFVLTKYLGVEAVDFQKTYDEALAFGDYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDAVGQGIRDRGAEYGASTGRPRRCGWMDIVALKRAV
AINGISGLCITKLDVLDGMEKLKVCIAYEYRGKRTEYAPLDAQGWEECTPVYLEFPGWTENTHGITEWDKLPVAARAYLR
ALEELAGCPISIVSTGPDRDHTMVLQDPFA

Sequences:

>Translated_430_residues
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGILRDDALCLIGNGVVISPAAL
IKEVSELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREKAAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEE
LLRTALDYHNFVLTKYLGVEAVDFQKTYDEALAFGDYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDAVGQGIRDRGAEYGASTGRPRRCGWMDIVALKRAV
AINGISGLCITKLDVLDGMEKLKVCIAYEYRGKRTEYAPLDAQGWEECTPVYLEFPGWTENTHGITEWDKLPVAARAYLR
ALEELAGCPISIVSTGPDRDHTMVLQDPFA
>Mature_429_residues
GQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGILRDDALCLIGNGVVISPAALI
KEVSELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREKAAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEEL
LRTALDYHNFVLTKYLGVEAVDFQKTYDEALAFGDYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVTS
SNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDAVGQGIRDRGAEYGASTGRPRRCGWMDIVALKRAVA
INGISGLCITKLDVLDGMEKLKVCIAYEYRGKRTEYAPLDAQGWEECTPVYLEFPGWTENTHGITEWDKLPVAARAYLRA
LEELAGCPISIVSTGPDRDHTMVLQDPFA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=41.3551401869159, Blast_Score=321, Evalue=7e-88,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=40.6976744186046, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=39.4472361809045, Blast_Score=282, Evalue=4e-76,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=60.6976744186046, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=43.0875576036866, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=43.0875576036866, Blast_Score=337, Evalue=8e-93,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=42.2897196261682, Blast_Score=334, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21356033, Length=418, Percent_Identity=41.6267942583732, Blast_Score=322, Evalue=3e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46178; Mature: 46047

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCEEEECCCCEEEEECCCCC
LRDDALCLIGNGVVISPAALIKEVSELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREK
CCCCCEEEECCCEEECHHHHHHHHHHHHHCCHHHHHCEEECCCCCCEEEHHHHHHHHHHH
AAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEELLRTALDYHNFVLTKYLGVE
HCCCCEECCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCE
AVDFQKTYDEALAFGDYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDAVGQGIRDRGAEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCCCCC
STGRPRRCGWMDIVALKRAVAINGISGLCITKLDVLDGMEKLKVCIAYEYRGKRTEYAPL
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHEEEEEEEECCCCCCCCCC
DAQGWEECTPVYLEFPGWTENTHGITEWDKLPVAARAYLRALEELAGCPISIVSTGPDRD
CCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
HTMVLQDPFA
CEEEEECCCC
>Mature Secondary Structure 
GQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCEEEECCCCEEEEECCCCC
LRDDALCLIGNGVVISPAALIKEVSELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREK
CCCCCEEEECCCEEECHHHHHHHHHHHHHCCHHHHHCEEECCCCCCEEEHHHHHHHHHHH
AAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEELLRTALDYHNFVLTKYLGVE
HCCCCEECCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCE
AVDFQKTYDEALAFGDYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDAVGQGIRDRGAEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCCCCC
STGRPRRCGWMDIVALKRAVAINGISGLCITKLDVLDGMEKLKVCIAYEYRGKRTEYAPL
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHEEEEEEEECCCCCCCCCC
DAQGWEECTPVYLEFPGWTENTHGITEWDKLPVAARAYLRALEELAGCPISIVSTGPDRD
CCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
HTMVLQDPFA
CEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA