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The map label for this gene is pdhB [H]

Identifier: 84622313

GI number: 84622313

Start: 708090

End: 709883

Strand: Direct

Name: pdhB [H]

Synonym: XOO_0656

Alternate gene names: 84622313

Gene position: 708090-709883 (Clockwise)

Preceding gene: 84622312

Following gene: 84622314

Centisome position: 14.33

GC content: 67.06

Gene sequence:

>1794_bases
ATGGCCGAAATCAAGGAAGCACTTGTCCCCGATATCGGTGACTACAGCGACGTCCCGGTAATCGAGGTGCTGGTGTCCGT
CGGCGATACGGTCAGCAAGGACCAGAGCCTGGTCACGCTGGAATCGGACAAGGCCACCATGGAAGTGCCGTCGTCGGTGT
CCGGCGTGGTCAAGGAGATCAAGGTGAAGCTGGGTGATTCGCTCTCGCAGGGCGCGCTGGTGGCATTGATCGAAGTGGCC
GATGCCGGCGTCGATGCCGGCGCTGCTGCTGCAGCCAAGCCCGCAGCTGCTGCTGCTGCGCCCGCCGCGCCGGCCAAGGC
CGCTCCTGCGTCTGCCCCGGCACCCGCGGCCAAGGCCGAAGCCGCTGCGCCTGCTGCCTCCTCGAACGGCGGCCTGACCG
AAGCGCGCGTGCCCGATATCGGCGATTACACCGACATCCCGGTGATCGAAGTGCTGGTCGCTGTCGGCGATACCGTCGCC
AAGGACCAGAGCCTGGTCACCCTGGAATCGGACAAGGCCACGATGGAAGTGCCGTCCTCGGCGGCCGGCGTGGTCAAGGA
GCTCAAGGTCAAGGTCGGCGACCTGCTCTCGCAGGGCAGTGTGGTGGCAATCATTGCAGCCAGCGATGGCGGTGCGGGGG
CGGCGCAGTCGTCCGTCAAGCCCACCACCGACACCGCCGAGACGGCGGGCAAGGTCGAGCCGGTCGCCGTGCCGGCCGAG
CCGGACAAGCTGGCCCAGCGCGAAATTGCGCAGGTGCAGGGCGCCCGTTCCGGCGCAGCTGCGCAGTCCGCGCAGGTCAG
CCAGCCGTCGGCCGGCAACCCGAGCAGCCCGCCGGTGACCTTCGATGCCGACAGCGTGCTGCCGTCCAAGGTGCCGTACG
CCAGCCCGGTGGTGCGCGTGTTCGCACGCGAACTGGGCGTAGACCTGAACCAGCTCAAGGGCAGTGAAAAGGGCGGCCGC
ATCACCCGCGAAGACGTGCAGCGCTTTGTGAAGGCGGCACTCAGCGGCGGCGCACCTGCCGCCGCCGGCGCTGTGCCGGC
CGGTGGCGCCAACGGCCTGAACCTGCTGGCCTGGCCGAAGGTGGACTTCAGCAAGTTCGGCGAGACCGAAACCCAGCCGC
TGTCGCGCATCAAGAAGATTTCCGGAGCCAACCTGGCGCGCAACTGGGCCATGATTCCGCACGTCACCCAGTTCGAGTCG
GCCGACATCACCGACCTGGAAGCCCTGCGCGTGGCCCTCAACAAGGAAAACGAGAAGGCGGGCATCAAGCTCACCATGCT
CGCCTTCCTGGTCAAGGCCAGCGCCGCGGCGTTGAAGACGTTCCCCGAGTTCAACGCCTCGCTGGATGCGGCCGGCGAAA
ACCTCACGCTGAAGAAGTACATCAACATCGGCTTCGCGGCCGATACACCGAATGGCCTGGTGGTGCCGGTGATCCGCGAC
GTCGACAAGAAGGGCGTGCTGCAGATCGCGCAGGAAAGCGGCGAACTGGCCAAGAAGGCGCGCGACGGCAAGCTGGGTCC
GGCCGACATGAGCGGCGGCTGCTTCTCGATCAGCTCGCTCGGCGGCATCGGCGGTACCGCGTTCACGCCGATCATCAATG
CGCCGGAAGTGGCGATCCTGGGCGTGTCCAAGTCGGCGATGCAGCCGGTCTGGAACGGCAAGGAGTTCGTGCCCAAGCTG
ATGCTGCCGTTGTCGCTGAGCTACGACCACCGGGTCATCGACGGCGCGCTGGCGGCCCGTTTCACCACCTACCTCAGCCA
GGTGCTGGCCGACATGCGTCGCGTCCTGCTGTGA

Upstream 100 bases:

>100_bases
TCCCGCCGGCCGAGCGATTTTTGCACGGCAATGGTTGGCGCTGCGGCCCGCAGCCGGCCACAATAGGGGTTTCCCCATTC
TGTTGCCGGAGTTCCCCCGT

Downstream 100 bases:

>100_bases
GGGCTGCGCTGCTGACCATCGCCGTGCTCGGTGTACTGCCGTGGACAACGGCAGTTGCACGCGAATGCGGGAGCACGCTC
GGGCGCGGCTGGCCGCCGGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVSGVVKEIKVKLGDSLSQGALVALIEVA
DAGVDAGAAAAAKPAAAAAAPAAPAKAAPASAPAPAAKAEAAAPAASSNGGLTEARVPDIGDYTDIPVIEVLVAVGDTVA
KDQSLVTLESDKATMEVPSSAAGVVKELKVKVGDLLSQGSVVAIIAASDGGAGAAQSSVKPTTDTAETAGKVEPVAVPAE
PDKLAQREIAQVQGARSGAAAQSAQVSQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGR
ITREDVQRFVKAALSGGAPAAAGAVPAGGANGLNLLAWPKVDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFES
ADITDLEALRVALNKENEKAGIKLTMLAFLVKASAAALKTFPEFNASLDAAGENLTLKKYINIGFAADTPNGLVVPVIRD
VDKKGVLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKSAMQPVWNGKEFVPKL
MLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL

Sequences:

>Translated_597_residues
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVSGVVKEIKVKLGDSLSQGALVALIEVA
DAGVDAGAAAAAKPAAAAAAPAAPAKAAPASAPAPAAKAEAAAPAASSNGGLTEARVPDIGDYTDIPVIEVLVAVGDTVA
KDQSLVTLESDKATMEVPSSAAGVVKELKVKVGDLLSQGSVVAIIAASDGGAGAAQSSVKPTTDTAETAGKVEPVAVPAE
PDKLAQREIAQVQGARSGAAAQSAQVSQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGR
ITREDVQRFVKAALSGGAPAAAGAVPAGGANGLNLLAWPKVDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFES
ADITDLEALRVALNKENEKAGIKLTMLAFLVKASAAALKTFPEFNASLDAAGENLTLKKYINIGFAADTPNGLVVPVIRD
VDKKGVLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKSAMQPVWNGKEFVPKL
MLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL
>Mature_596_residues
AEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVSGVVKEIKVKLGDSLSQGALVALIEVAD
AGVDAGAAAAAKPAAAAAAPAAPAKAAPASAPAPAAKAEAAAPAASSNGGLTEARVPDIGDYTDIPVIEVLVAVGDTVAK
DQSLVTLESDKATMEVPSSAAGVVKELKVKVGDLLSQGSVVAIIAASDGGAGAAQSSVKPTTDTAETAGKVEPVAVPAEP
DKLAQREIAQVQGARSGAAAQSAQVSQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGRI
TREDVQRFVKAALSGGAPAAAGAVPAGGANGLNLLAWPKVDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFESA
DITDLEALRVALNKENEKAGIKLTMLAFLVKASAAALKTFPEFNASLDAAGENLTLKKYINIGFAADTPNGLVVPVIRDV
DKKGVLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKSAMQPVWNGKEFVPKLM
LPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=481, Percent_Identity=28.4823284823285, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI31711992, Length=332, Percent_Identity=33.1325301204819, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=36.3636363636364, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI203098816, Length=230, Percent_Identity=33.9130434782609, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI203098753, Length=216, Percent_Identity=35.1851851851852, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=38.562091503268, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786305, Length=309, Percent_Identity=60.1941747572816, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1786946, Length=445, Percent_Identity=30.7865168539326, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=468, Percent_Identity=27.7777777777778, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=214, Percent_Identity=40.6542056074766, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560088, Length=462, Percent_Identity=27.9220779220779, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=34.0909090909091, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=238, Percent_Identity=34.8739495798319, Blast_Score=143, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=335, Percent_Identity=30.1492537313433, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18859875, Length=308, Percent_Identity=33.7662337662338, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.618025751073, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=33.0396475770925, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24645909, Length=209, Percent_Identity=35.4066985645933, Blast_Score=116, Evalue=6e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 60853; Mature: 60722

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVSGVVKEI
CCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHH
KVKLGDSLSQGALVALIEVADAGVDAGAAAAAKPAAAAAAPAAPAKAAPASAPAPAAKAE
HHHHCCCCCCCCEEEEEEHHHCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHH
AAAPAASSNGGLTEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLESDKATMEVPSS
HCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCH
AAGVVKELKVKVGDLLSQGSVVAIIAASDGGAGAAQSSVKPTTDTAETAGKVEPVAVPAE
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCC
PDKLAQREIAQVQGARSGAAAQSAQVSQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRV
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
FARELGVDLNQLKGSEKGGRITREDVQRFVKAALSGGAPAAAGAVPAGGANGLNLLAWPK
HHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECC
VDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFESADITDLEALRVALNKENEKA
CCHHHCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
GIKLTMLAFLVKASAAALKTFPEFNASLDAAGENLTLKKYINIGFAADTPNGLVVPVIRD
CCHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHC
VDKKGVLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAIL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCCCHHCCCCCCCCEEEE
GVSKSAMQPVWNGKEFVPKLMLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL
ECCHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVSGVVKEI
CCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHH
KVKLGDSLSQGALVALIEVADAGVDAGAAAAAKPAAAAAAPAAPAKAAPASAPAPAAKAE
HHHHCCCCCCCCEEEEEEHHHCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHH
AAAPAASSNGGLTEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLESDKATMEVPSS
HCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCH
AAGVVKELKVKVGDLLSQGSVVAIIAASDGGAGAAQSSVKPTTDTAETAGKVEPVAVPAE
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCC
PDKLAQREIAQVQGARSGAAAQSAQVSQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRV
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
FARELGVDLNQLKGSEKGGRITREDVQRFVKAALSGGAPAAAGAVPAGGANGLNLLAWPK
HHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECC
VDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFESADITDLEALRVALNKENEKA
CCHHHCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
GIKLTMLAFLVKASAAALKTFPEFNASLDAAGENLTLKKYINIGFAADTPNGLVVPVIRD
CCHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHC
VDKKGVLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAIL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCCCHHCCCCCCCCEEEE
GVSKSAMQPVWNGKEFVPKLMLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL
ECCHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]