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The map label for this gene is cheR [C]

Identifier: 84622250

GI number: 84622250

Start: 642318

End: 643805

Strand: Direct

Name: cheR [C]

Synonym: XOO_0593

Alternate gene names: 84622250

Gene position: 642318-643805 (Clockwise)

Preceding gene: 84622248

Following gene: 84622252

Centisome position: 13.0

GC content: 62.16

Gene sequence:

>1488_bases
GTGCATACAGGCGTTGGGAGACAAGTGCTGCATGGCGGTCTGCCGTGCTTTGAGCAATCATCGCCGATTGCGCCTGAGCT
CAGCGTTGCGAACGATAGTGCCGCCATGACCGACGACGAAATTTCCAGCACCGAGACCGCCCCTGACATCGCCACGCAGC
CGCCCTTATTCGTGCTGGGCGTGGGCGCCGCGCGGCTTGATGCAGGCCGCTTGGCAGAATTGCTCGATGCGGTAGCAGGC
GCTGCGAATCTGGCGATCATGCTGCTGCTGCGCGATCGCCAGTTGCTGGACGAGGCGCACCTGCGCGCATTGCTTGGTAA
ACAAGCCACGCTGTTGTCGGTGCCCTTCGATGGCGAGAAATTGCAGCCGGGGCGCTTCTATCTACCGCCTGCCGATACCG
TGGTCACGCTGGAGGAAGGGCGTATCCGCCTGCGGGCATTGCCCAACGGCGAAAGCGACCACGGGTTGATCGACAGTTTC
TTCGTGTCGATGGCGCACGATCAGGACGGCAATGTGATCGGTCTGATCATGGGGCGTTTCGACGGCGACGGCACGCTGGG
CACGGCAGCGATCAAGGAATGCGGCGGCCTGGCGCTGGCCGTGGACGATGCCTCGCTGCATGGGCTGGATCTGCGCAGCG
CAAATAATCCGGCTGCCATCACCGACGACATCCTGCCGCTGCCACAAGTGGCTGCACGCATCGCTGCGCACATGCAGCGT
CACCACGGCTTCGGTCATCCGGGACCGCTGGGCCTGTGCAATGGCAGCGAGAGCGACAAGCTCAGCCAGATTGCCGCGAT
CCTGCGCAATACCACCGGCCACGATTTTCACGGTTACAAACGCGCCACGTTCCTGCGTCGCGTGCAGCAGCGCATGCAGG
TCGCGCAGGTGGAAATGCTTGAGCAGTATCTGCACATCCTGCGCTACCGCTTCGACGAGCCGTTGCAGCTGTTCAACGAC
CTGCTGATTGGCGTGACCAAGTTTTTTCGCGACCGCCGCGGATTCGAATTGCTCGAGCAGCAGGTGATCCCGCGGTTGTT
TCAGGGCAAGCACACCGACGACAGCATGCGCGTCTGGGTGCTGGGTTGCTCCACCGGCGAAGAAGCCTATTCGCTGGCGA
TGCTGCTGCGCGAGCATGCCGAAACCCTGGACAGCGCGCCACGTATCCAGATCTTCGCCAGCGATATCGACGGGCGTGCG
CTGGCCACCGCGCGGGTGGGGCGCTATGCCTCCAGCATCGTGGGCGAGGTTGCGCTTGAGCGCTTGCGGCGCTGGTTCGT
CAAGGAAGGCGACACCTATGTGGTGAACAAGGAACTGCGCGAGCTGTGCGTGTTCTCCCAGCACAGTATCGTCAAGGATC
CGCCGTTTTCGCGGCTGGATCTGGTCTCGTGCCGCAATCTGCTGATCTATCTGGATGCCGAATTGCAGAATCGGGTCATC
CCGATCTTTCATTTCGGCATCCGCCCGGGCGGCTATCTGTTTTCCTGA

Upstream 100 bases:

>100_bases
CGCGTCAGACGACGCGCGTGCGAATGGTGCCTGGATCGCAGGTTAACGCGGCGCGTAAGCACGCCCTTCCAAGACCGGTC
TCCTCAATGCAGACACGCGA

Downstream 100 bases:

>100_bases
TTAGCCCTGACCTTCAGCTGCAGCACCTCCCAGCTTATCGCGAAACCCGCCCAATGCCAGGCCGCCATGGGGTCTTGGCT
GAAAGAACGTCGCACCGTCT

Product: putative methyltransferase

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MHTGVGRQVLHGGLPCFEQSSPIAPELSVANDSAAMTDDEISSTETAPDIATQPPLFVLGVGAARLDAGRLAELLDAVAG
AANLAIMLLLRDRQLLDEAHLRALLGKQATLLSVPFDGEKLQPGRFYLPPADTVVTLEEGRIRLRALPNGESDHGLIDSF
FVSMAHDQDGNVIGLIMGRFDGDGTLGTAAIKECGGLALAVDDASLHGLDLRSANNPAAITDDILPLPQVAARIAAHMQR
HHGFGHPGPLGLCNGSESDKLSQIAAILRNTTGHDFHGYKRATFLRRVQQRMQVAQVEMLEQYLHILRYRFDEPLQLFND
LLIGVTKFFRDRRGFELLEQQVIPRLFQGKHTDDSMRVWVLGCSTGEEAYSLAMLLREHAETLDSAPRIQIFASDIDGRA
LATARVGRYASSIVGEVALERLRRWFVKEGDTYVVNKELRELCVFSQHSIVKDPPFSRLDLVSCRNLLIYLDAELQNRVI
PIFHFGIRPGGYLFS

Sequences:

>Translated_495_residues
MHTGVGRQVLHGGLPCFEQSSPIAPELSVANDSAAMTDDEISSTETAPDIATQPPLFVLGVGAARLDAGRLAELLDAVAG
AANLAIMLLLRDRQLLDEAHLRALLGKQATLLSVPFDGEKLQPGRFYLPPADTVVTLEEGRIRLRALPNGESDHGLIDSF
FVSMAHDQDGNVIGLIMGRFDGDGTLGTAAIKECGGLALAVDDASLHGLDLRSANNPAAITDDILPLPQVAARIAAHMQR
HHGFGHPGPLGLCNGSESDKLSQIAAILRNTTGHDFHGYKRATFLRRVQQRMQVAQVEMLEQYLHILRYRFDEPLQLFND
LLIGVTKFFRDRRGFELLEQQVIPRLFQGKHTDDSMRVWVLGCSTGEEAYSLAMLLREHAETLDSAPRIQIFASDIDGRA
LATARVGRYASSIVGEVALERLRRWFVKEGDTYVVNKELRELCVFSQHSIVKDPPFSRLDLVSCRNLLIYLDAELQNRVI
PIFHFGIRPGGYLFS
>Mature_495_residues
MHTGVGRQVLHGGLPCFEQSSPIAPELSVANDSAAMTDDEISSTETAPDIATQPPLFVLGVGAARLDAGRLAELLDAVAG
AANLAIMLLLRDRQLLDEAHLRALLGKQATLLSVPFDGEKLQPGRFYLPPADTVVTLEEGRIRLRALPNGESDHGLIDSF
FVSMAHDQDGNVIGLIMGRFDGDGTLGTAAIKECGGLALAVDDASLHGLDLRSANNPAAITDDILPLPQVAARIAAHMQR
HHGFGHPGPLGLCNGSESDKLSQIAAILRNTTGHDFHGYKRATFLRRVQQRMQVAQVEMLEQYLHILRYRFDEPLQLFND
LLIGVTKFFRDRRGFELLEQQVIPRLFQGKHTDDSMRVWVLGCSTGEEAYSLAMLLREHAETLDSAPRIQIFASDIDGRA
LATARVGRYASSIVGEVALERLRRWFVKEGDTYVVNKELRELCVFSQHSIVKDPPFSRLDLVSCRNLLIYLDAELQNRVI
PIFHFGIRPGGYLFS

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=246, Percent_Identity=29.6747967479675, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80 [C]

Molecular weight: Translated: 54495; Mature: 54495

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00092 N6_MTASE ; PS50123 CHER ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTGVGRQVLHGGLPCFEQSSPIAPELSVANDSAAMTDDEISSTETAPDIATQPPLFVLG
CCCCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
VGAARLDAGRLAELLDAVAGAANLAIMLLLRDRQLLDEAHLRALLGKQATLLSVPFDGEK
CCHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
LQPGRFYLPPADTVVTLEEGRIRLRALPNGESDHGLIDSFFVSMAHDQDGNVIGLIMGRF
CCCCEEECCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEE
DGDGTLGTAAIKECGGLALAVDDASLHGLDLRSANNPAAITDDILPLPQVAARIAAHMQR
CCCCCHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HHGFGHPGPLGLCNGSESDKLSQIAAILRNTTGHDFHGYKRATFLRRVQQRMQVAQVEML
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EQYLHILRYRFDEPLQLFNDLLIGVTKFFRDRRGFELLEQQVIPRLFQGKHTDDSMRVWV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
LGCSTGEEAYSLAMLLREHAETLDSAPRIQIFASDIDGRALATARVGRYASSIVGEVALE
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RLRRWFVKEGDTYVVNKELRELCVFSQHSIVKDPPFSRLDLVSCRNLLIYLDAELQNRVI
HHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEECHHCCCEE
PIFHFGIRPGGYLFS
EEEEECCCCCCCCCC
>Mature Secondary Structure
MHTGVGRQVLHGGLPCFEQSSPIAPELSVANDSAAMTDDEISSTETAPDIATQPPLFVLG
CCCCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
VGAARLDAGRLAELLDAVAGAANLAIMLLLRDRQLLDEAHLRALLGKQATLLSVPFDGEK
CCHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
LQPGRFYLPPADTVVTLEEGRIRLRALPNGESDHGLIDSFFVSMAHDQDGNVIGLIMGRF
CCCCEEECCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEE
DGDGTLGTAAIKECGGLALAVDDASLHGLDLRSANNPAAITDDILPLPQVAARIAAHMQR
CCCCCHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HHGFGHPGPLGLCNGSESDKLSQIAAILRNTTGHDFHGYKRATFLRRVQQRMQVAQVEML
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EQYLHILRYRFDEPLQLFNDLLIGVTKFFRDRRGFELLEQQVIPRLFQGKHTDDSMRVWV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
LGCSTGEEAYSLAMLLREHAETLDSAPRIQIFASDIDGRALATARVGRYASSIVGEVALE
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RLRRWFVKEGDTYVVNKELRELCVFSQHSIVKDPPFSRLDLVSCRNLLIYLDAELQNRVI
HHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEECHHCCCEE
PIFHFGIRPGGYLFS
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]

Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]