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The map label for this gene is bioF [H]

Identifier: 84622077

GI number: 84622077

Start: 452033

End: 453238

Strand: Direct

Name: bioF [H]

Synonym: XOO_0420

Alternate gene names: 84622077

Gene position: 452033-453238 (Clockwise)

Preceding gene: 84622076

Following gene: 84622078

Centisome position: 9.15

GC content: 67.16

Gene sequence:

>1206_bases
ATGGCTCGCCCCGATCTGCACGGACGGATTTCGTCGCTACGTAAATTGCATGTGGCCCAGGACCGGGTGCGGGTGCGGCG
ACAGGTCGGCCGCCGCGATGGCGTGCGGCTGGAAATCGACGGCCGCTGGCTGACCGGGTTCTGCTCCAACGACTATCTAG
GCCTGTCGCAACAGTTCGAAGTGATCGCCGCGCTGCAGGATGCCGCTGCCCGCGATGGCGCCGGTGCCACCGCCTCGCAT
CTGATCTGCGGCCACCACACCGCGCACGAAACGCTTGAGCGCGAAATCGCCGAGTGGCTGGGCTATCCATCGGCGTTGCT
GTTCGGCAACGGTTTCATCGCCAATCTGGCGGTGCAACAGGCACTGCTCAGCGAAGAAGACGATGTCTGCGTGCAGGACC
GGCTCAATCACGCCAGCCTGCTCGACGCCACACGGCTGGCTGGCTGCCGGCTGCGACGCTATCCACATCTGGATGTGGAA
GGCGCGATGCGTCAGCTCAAAGGCGCGCCCGAGGGTGCGGCGATGCTGGCCAGCGACGCTGTTTTCAGCATGGATGGCGA
TGTCGCGCCGCTGCGCGCGTTGAGCCTGGTGGCCCGCATGCAGGACGCCCTGTTTTATGTGGACGACGCGCACGGTGTTG
GCGTGCTCGGCCCCCAGGGTCGCGGCTGCGTGGCCGATGCCGGGCTGGGCGTGGCCGAAGTGCCGTTGCAGTTGGTGACC
CTGGGCAAAGCGCTCGGTGGCTACGGCGCGGTTGTTGTTGGCGACGACGCGCTGGTGCGCCATCTGGCCGAAACCGCGCG
CCCGTACATTTACACCACTGCATTGCCACCGGCCCAAGTCGCCGCCACGCTGGCAGCCGTGCGCCTGGCGCGTCGCGACG
ATTGGCGGCGTGCGCGCCTGGTCGAATTGATTGCTGCGTTCCGCGATGGCGCGCGCAAGCATGGCTTCGAACTGATGGCG
TCCGACACGCCGATCCAGCCCTTGCTGTGCGGTGAAGAAGCGACCGTCATGGCGATGTCGGCCGCACTGGAACAGGCGGG
TTTCATGGTCGGCGCCATTCGTCCGCCCACCGTGCCCGAAGGCAAGGCGCGCTTGCGCGTGACGCTGTCCGCGCTGCATA
CGCCGCAGCAGGTGCAGGCGCTGATCGACGCTATCGTGCAGGCGCGCGATGTGGTGAGCCGGCAGCCGCTGCGTGCGTCT
GCCTGA

Upstream 100 bases:

>100_bases
ACCGCCTGAGCCGGCGTCCGGCACCGGTCGTCGGTGCCGGCTGGGCGCGCTGAGCCCGGCACGCTACGCTAGCGGCCCTT
CTCTGCCGTTGAACGCTCCC

Downstream 100 bases:

>100_bases
GACAAGCTGTGGCTTGTGCATGGCCTGCGCTGAAACAAGCGTGGGTCACTCAACGAGTGCATTGATTGCATGACGCATGC
GCGTGGAAGGCGACAACCAT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MARPDLHGRISSLRKLHVAQDRVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVIAALQDAAARDGAGATASH
LICGHHTAHETLEREIAEWLGYPSALLFGNGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVE
GAMRQLKGAPEGAAMLASDAVFSMDGDVAPLRALSLVARMQDALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
LGKALGGYGAVVVGDDALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARLVELIAAFRDGARKHGFELMA
SDTPIQPLLCGEEATVMAMSAALEQAGFMVGAIRPPTVPEGKARLRVTLSALHTPQQVQALIDAIVQARDVVSRQPLRAS
A

Sequences:

>Translated_401_residues
MARPDLHGRISSLRKLHVAQDRVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVIAALQDAAARDGAGATASH
LICGHHTAHETLEREIAEWLGYPSALLFGNGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVE
GAMRQLKGAPEGAAMLASDAVFSMDGDVAPLRALSLVARMQDALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
LGKALGGYGAVVVGDDALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARLVELIAAFRDGARKHGFELMA
SDTPIQPLLCGEEATVMAMSAALEQAGFMVGAIRPPTVPEGKARLRVTLSALHTPQQVQALIDAIVQARDVVSRQPLRAS
A
>Mature_400_residues
ARPDLHGRISSLRKLHVAQDRVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVIAALQDAAARDGAGATASHL
ICGHHTAHETLEREIAEWLGYPSALLFGNGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEG
AMRQLKGAPEGAAMLASDAVFSMDGDVAPLRALSLVARMQDALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVTL
GKALGGYGAVVVGDDALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARLVELIAAFRDGARKHGFELMAS
DTPIQPLLCGEEATVMAMSAALEQAGFMVGAIRPPTVPEGKARLRVTLSALHTPQQVQALIDAIVQARDVVSRQPLRASA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=369, Percent_Identity=35.7723577235772, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI284448556, Length=347, Percent_Identity=36.5994236311239, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI83977440, Length=355, Percent_Identity=32.112676056338, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI83977444, Length=355, Percent_Identity=32.112676056338, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI83977442, Length=355, Percent_Identity=32.112676056338, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4502025, Length=377, Percent_Identity=30.2387267904509, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI40316939, Length=377, Percent_Identity=30.2387267904509, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=27.1232876712329, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI119220554, Length=362, Percent_Identity=25.1381215469613, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI5454084, Length=250, Percent_Identity=22, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786993, Length=354, Percent_Identity=42.9378531073446, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1790046, Length=352, Percent_Identity=34.9431818181818, Blast_Score=207, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI71994529, Length=368, Percent_Identity=35.8695652173913, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI32566772, Length=360, Percent_Identity=26.9444444444444, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17560914, Length=385, Percent_Identity=25.4545454545455, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=385, Percent_Identity=25.4545454545455, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982625, Length=283, Percent_Identity=22.6148409893993, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71982617, Length=283, Percent_Identity=22.6148409893993, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320438, Length=366, Percent_Identity=29.2349726775956, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320267, Length=372, Percent_Identity=22.8494623655914, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323954, Length=275, Percent_Identity=23.6363636363636, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24662918, Length=367, Percent_Identity=33.2425068119891, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI17137420, Length=396, Percent_Identity=30.5555555555556, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17136286, Length=381, Percent_Identity=25.7217847769029, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24653280, Length=280, Percent_Identity=24.2857142857143, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653276, Length=280, Percent_Identity=24.2857142857143, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653278, Length=280, Percent_Identity=24.2857142857143, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 43037; Mature: 42906

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPDLHGRISSLRKLHVAQDRVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCHHHHH
VIAALQDAAARDGAGATASHLICGHHTAHETLEREIAEWLGYPSALLFGNGFIANLAVQQ
HHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHH
ALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEGAMRQLKGAPEGAAMLASDA
HHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHCCC
VFSMDGDVAPLRALSLVARMQDALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCCCCCEECCCCCHHHHHHHHHH
LGKALGGYGAVVVGDDALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARL
HHHHHCCCCEEEECCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
VELIAAFRDGARKHGFELMASDTPIQPLLCGEEATVMAMSAALEQAGFMVGAIRPPTVPE
HHHHHHHHCCHHHCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCC
GKARLRVTLSALHTPQQVQALIDAIVQARDVVSRQPLRASA
CCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARPDLHGRISSLRKLHVAQDRVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCHHHHH
VIAALQDAAARDGAGATASHLICGHHTAHETLEREIAEWLGYPSALLFGNGFIANLAVQQ
HHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHH
ALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEGAMRQLKGAPEGAAMLASDA
HHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHCCC
VFSMDGDVAPLRALSLVARMQDALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCCCCCEECCCCCHHHHHHHHHH
LGKALGGYGAVVVGDDALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARL
HHHHHCCCCEEEECCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
VELIAAFRDGARKHGFELMASDTPIQPLLCGEEATVMAMSAALEQAGFMVGAIRPPTVPE
HHHHHHHHCCHHHCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCC
GKARLRVTLSALHTPQQVQALIDAIVQARDVVSRQPLRASA
CCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]