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The map label for this gene is bioB [H]

Identifier: 84622076

GI number: 84622076

Start: 450907

End: 451941

Strand: Direct

Name: bioB [H]

Synonym: XOO_0419

Alternate gene names: 84622076

Gene position: 450907-451941 (Clockwise)

Preceding gene: 84622074

Following gene: 84622077

Centisome position: 9.13

GC content: 65.99

Gene sequence:

>1035_bases
ATGTCTGTTGTCGTCCGCCATGACTGGGATCACAAAGAGCTGCAGGCGCTGTTCGATCTGCCGTTTCCGGAGCTGCTGCA
CCGCGCCGCCAGCGTGCATCGCGCCCATTTCGATCCGGCTGAAGTGCAGGTCTCCACACTGCTGTCGGTCAAGACCGGCG
GTTGCCCGGAAGACTGCGCGTATTGCCCGCAGGCGCAGCGCTACGACACCGGCGTGACCGCGCAGAAGCTGCTGGAGACC
GAAGAGGTCGTGGCCAAGGCGCGTCAGGCCAAGGCGGCTGGCGCCTCGCGTTTCTGCATGGGCGCCGCCTGGCGCTCGCC
CAAGGAGCGCGACATCCCCAAGGTGGCCGCGATGATCCGCGAGGTGAAAGCCATGGGTCTGGAAACCTGCGCCACGCTCG
GCATGCTCGACGCCGGCCAGGCGCGTGCGCTCAAGGATGCCGGGCTGGACTACTACAACCACAATCTGGACACCGCGCCG
GATTATTACGACACCATCATTCACACCCGCCAGTACCAGGACCGCCTGAACACGCTGGAGCATGTCCGCGATGTCGGTCT
GAAGACCTGCTGCGGCGGCATCGTCGGCATGGGCGAAACCCGCGACCACCGCATCGGCCTGCTGCTGGCGCTGGCCACGC
TGCCGGCGCATCCGGATTCGGTGCCGATCAATCAGCTGGTGCAGGTGCCCGGTACTCCACTGCACGGCACCCAGCAGCTG
GACCCGTTCGAGTTCGTGCGCATGATCGCGGTTGCGCGCATCGCCATGCCCAAGTCGATGGTGCGCCTGTCCGCCGGCCG
CGAAGCAATGAGCGACGAACTGCAGGCGCTGTGCTTCCTGGCCGGTGCCAATTCGATCTTCTACGGCGAAAAACTGCTCA
CCACCGGCAACCCGGATACCGAGCGCGACCAGACGCTGTTCCAGCGCCTGGGTCTGCGCCCGATGCAGATCACCGTCGAT
GCGGCCGAGCACGATCACCCCGCTACCGTGCATGCGGAGATCACCCGCAGCGCGGCCTGCGAGCATACCGCCTGA

Upstream 100 bases:

>100_bases
CGGCCACCTGTAAACCGATCGCACCTGATTGACGTTGACAGCATCTCCCATGCTCACCAGACTGCCTGGCTTTCAAGCCG
CTGACTGTCAGGAAACTCCG

Downstream 100 bases:

>100_bases
GCCGGCGTCCGGCACCGGTCGTCGGTGCCGGCTGGGCGCGCTGAGCCCGGCACGCTACGCTAGCGGCCCTTCTCTGCCGT
TGAACGCTCCCATGGCTCGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSVVVRHDWDHKELQALFDLPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVTAQKLLET
EEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAP
DYYDTIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETRDHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
DPFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQTLFQRLGLRPMQITVD
AAEHDHPATVHAEITRSAACEHTA

Sequences:

>Translated_344_residues
MSVVVRHDWDHKELQALFDLPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVTAQKLLET
EEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAP
DYYDTIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETRDHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
DPFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQTLFQRLGLRPMQITVD
AAEHDHPATVHAEITRSAACEHTA
>Mature_343_residues
SVVVRHDWDHKELQALFDLPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVTAQKLLETE
EVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPD
YYDTIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETRDHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQLD
PFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQTLFQRLGLRPMQITVDA
AEHDHPATVHAEITRSAACEHTA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=321, Percent_Identity=60.1246105919003, Blast_Score=417, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=45.5974842767296, Blast_Score=322, Evalue=6e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37802; Mature: 37670

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVVRHDWDHKELQALFDLPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCA
CEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHH
YCPQAQRYDTGVTAQKLLETEEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHH
EVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPDYYDTIIHTRQYQDRLNTLE
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HVRDVGLKTCCGGIVGMGETRDHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCC
DPFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDT
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCC
ERDQTLFQRLGLRPMQITVDAAEHDHPATVHAEITRSAACEHTA
HHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVVVRHDWDHKELQALFDLPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCA
EEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHH
YCPQAQRYDTGVTAQKLLETEEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHH
EVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPDYYDTIIHTRQYQDRLNTLE
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HVRDVGLKTCCGGIVGMGETRDHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCC
DPFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDT
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCC
ERDQTLFQRLGLRPMQITVDAAEHDHPATVHAEITRSAACEHTA
HHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]