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The map label for this gene is dusA [H]

Identifier: 84622040

GI number: 84622040

Start: 415583

End: 416587

Strand: Direct

Name: dusA [H]

Synonym: XOO_0383

Alternate gene names: 84622040

Gene position: 415583-416587 (Clockwise)

Preceding gene: 84622039

Following gene: 84622041

Centisome position: 8.41

GC content: 67.66

Gene sequence:

>1005_bases
ATGACCGCTTCCGCCGATCGCTACGCCGCCTCCCTGCGCCTGTCCGTCGCTCCGATGATGGACTGGACCGACCGTCATTG
CCGGGTCTTCCACCGTCTGTTGGCGCCGTCGGCACGCCTGTACACCGAGATGGTGCACGCCAATGCGGTGATCCACGGCG
ATCGCGCGCGGCTGCTCGGCTTCGATGCGGTCGAACATCCGCTGGCGCTGCAGCTGGGCGGCAGTGATCCGGCCTTGCTA
GCGCAGGCGGCGGCGATCGCGCAGGAGTGGGGATTTGATGAGATCAATCTCAATTGCGGGTGCCCGTCCGACCGCGTGCA
GGCCGGGCGGTTCGGCGCCTGCCTGATGCGCGAGCCGGCACTGGTGGCCGAATGCGTCGCTGCCATGTGCACGGCCACCC
ACGTGCCGATCACGGTCAAATGCCGTCTTGGCGTGGACGACGATGACGATTACGCGCTATTCGCCCGCTTTGTCGACCAG
GTGGTCGCGGCCGGCGCGGCGATGGTGGTGGTGCACGCCCGCAACGCCTGGCTCAAGGGCCTGTCGCCGAAGGAAAACCG
CGAAGTGCCGCCGCTGCGTTACGACTGGGCCTACCGGCTCAATCAGGAGCGGCCCGGCCTGCCTGTGGTGCTCAACGGTG
GCATCGTGGCCGTGGACGCCGCAATGGCGCATCTGCAGCATACCGATGGCGTCATGCTCGGCCGCGCGGCCTACCACGAC
CCCTACGTCCTGCATTGTCTGGACGCTGCCTTGAACCAGCGGCCCGAGCAACCGCGCGAGTCCCTGCTGCGCGCATTGCA
GCCGTACGTGGCATCGCAGCTGGCGCAAGGTCTTGCCCTGAAGCATATGACGCGGCACGTGCTGGGCCTGTTCCATGGCC
AGCCCGGCGGGCGGTCATTCCGGCAGGTCTTGAGTGAAGGCGCACATCGTCCGGGTGCCGGCTGGGAGTTGGTCGAACAG
GCGCTGCAGCGCACCGATACGCGATCCTGGCGGGTGGTCGCCTAG

Upstream 100 bases:

>100_bases
CCGGCCTGCCACTGCGCACCGCCGCAGATTCATCGACGCCCGTGTAAGCTGTTGATCCTGCTTCGCATTCTACTGGCTGC
TCGCTGCGGCCACTTCGTCG

Downstream 100 bases:

>100_bases
TTGGCCCACCGCTTGCGTTCCTGAACGGGCTGCTGGGATATCGATGGTTTCGAGGGTTTCCAGCAACGGGCGCGTCCGCC
ACAGCCCACGCCCACCATGG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLLGFDAVEHPLALQLGGSDPALL
AQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCTATHVPITVKCRLGVDDDDDYALFARFVDQ
VVAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLNQERPGLPVVLNGGIVAVDAAMAHLQHTDGVMLGRAAYHD
PYVLHCLDAALNQRPEQPRESLLRALQPYVASQLAQGLALKHMTRHVLGLFHGQPGGRSFRQVLSEGAHRPGAGWELVEQ
ALQRTDTRSWRVVA

Sequences:

>Translated_334_residues
MTASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLLGFDAVEHPLALQLGGSDPALL
AQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCTATHVPITVKCRLGVDDDDDYALFARFVDQ
VVAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLNQERPGLPVVLNGGIVAVDAAMAHLQHTDGVMLGRAAYHD
PYVLHCLDAALNQRPEQPRESLLRALQPYVASQLAQGLALKHMTRHVLGLFHGQPGGRSFRQVLSEGAHRPGAGWELVEQ
ALQRTDTRSWRVVA
>Mature_333_residues
TASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLLGFDAVEHPLALQLGGSDPALLA
QAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCTATHVPITVKCRLGVDDDDDYALFARFVDQV
VAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLNQERPGLPVVLNGGIVAVDAAMAHLQHTDGVMLGRAAYHDP
YVLHCLDAALNQRPEQPRESLLRALQPYVASQLAQGLALKHMTRHVLGLFHGQPGGRSFRQVLSEGAHRPGAGWELVEQA
LQRTDTRSWRVVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=233, Percent_Identity=29.1845493562232, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=52.5316455696203, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1788462, Length=221, Percent_Identity=31.6742081447964, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789660, Length=257, Percent_Identity=28.7937743190661, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=258, Percent_Identity=25.1937984496124, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=28.7425149700599, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=28.9256198347107, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=28.9256198347107, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36607; Mature: 36476

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLLG
CCCCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEECCCHHEEE
FDAVEHPLALQLGGSDPALLAQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPA
CHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHCCHH
LVAECVAAMCTATHVPITVKCRLGVDDDDDYALFARFVDQVVAAGAAMVVVHARNAWLKG
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCC
LSPKENREVPPLRYDWAYRLNQERPGLPVVLNGGIVAVDAAMAHLQHTDGVMLGRAAYHD
CCCCCCCCCCCCEECHHEEECCCCCCCCEEECCCEEEHHHHHHHHHHCCCEEECHHHCCC
PYVLHCLDAALNQRPEQPRESLLRALQPYVASQLAQGLALKHMTRHVLGLFHGQPGGRSF
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RQVLSEGAHRPGAGWELVEQALQRTDTRSWRVVA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure 
TASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLLG
CCCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEECCCHHEEE
FDAVEHPLALQLGGSDPALLAQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPA
CHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHCCHH
LVAECVAAMCTATHVPITVKCRLGVDDDDDYALFARFVDQVVAAGAAMVVVHARNAWLKG
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCC
LSPKENREVPPLRYDWAYRLNQERPGLPVVLNGGIVAVDAAMAHLQHTDGVMLGRAAYHD
CCCCCCCCCCCCEECHHEEECCCCCCCCEEECCCEEEHHHHHHHHHHCCCEEECHHHCCC
PYVLHCLDAALNQRPEQPRESLLRALQPYVASQLAQGLALKHMTRHVLGLFHGQPGGRSF
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RQVLSEGAHRPGAGWELVEQALQRTDTRSWRVVA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]