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The map label for this gene is hisS

Identifier: 84489457

GI number: 84489457

Start: 757663

End: 758943

Strand: Direct

Name: hisS

Synonym: Msp_0648

Alternate gene names: 84489457

Gene position: 757663-758943 (Clockwise)

Preceding gene: 84489455

Following gene: 84489458

Centisome position: 42.87

GC content: 28.65

Gene sequence:

>1281_bases
ATGGAATTTAATAAACCAAGAGGAACACGTGATTTTCTTTTTGAGGATATGGAAGAGCGAAATTATGTTGAAAATACTAT
AAGAAATGTTGTTGAAAATTATGGATTTAAGGAAATAAAAACACCAATATTTGAAGATTTAGAATTATTCACAACAAAAT
CTGGTGAAGGAATAAAAGAGGAAATATATCATTTTCAAGATAAAGGTGGAAGAGATTTAGCTTTAAGACCAGAACTTACA
GCAAGTGTAAGTAGATTATATAATAACAATCTTCAAAAATCTGCAAAACCACTGAAAATGTATTATTTTGGCAGTTGTTT
TAGATATGAAAGACCACAAGCAGGTAGATTTAGGGAATTTTGGCAGTTTGGTATAGAAGTTATTGGTGGAGTACCAATTT
ATAATGAAGCTGAAATTATAGCAATGGCTAATGAGGCATTAACTCAGGTTGGAATTAAAAATTATGAGATTAACATTGGA
CATCTTGGTATTATTAAAGGTGTTTTAAATCAATTAAATATTTCATCACAACAACAAACTGCCATAATTGCAAGTATAGA
TAAGGAAGACTATGAATTACTTGATAAATTATTAGAAGATAACGATATTTCAAGTGAATATAAAAATATAATTACAAGTA
TTATTAATATAAAAGGTAGTCGTAGTGATTTAGAAACATTAAAACAATTACTTGAAGATATACCTGAGAGTTTTGAAGCT
TTAGAAAATCTTGATAAAACATTAGAGGTTCTTGAAGTATTTGGTTTTGATGATTATGTTGTTAATTTATCAATAGCAAG
GGGACTTGATTATTATACTGGTATTGTATTTGAAATATATGTACCAGATTTAGGTGCTGAGAAACAAATTACAGGTGGAG
GAACATACAACCTCACAGCATTATTTGACTCAGAAAAAGTGGAATCTACAGGTTTTGCATTTGGTTTTGATAGAATAATG
GAAGCATACCATAGACAAAACATACAAGTACCTATAAGTTCTAAACCAAAAGTACTTGTTATTCCTGTGAAAAAAGAATT
TAAATTAAATGCTATAGAAGTAGCACAACAATTACGAGCCCATAATATTGTTACAGATATAGATCTTAAGGGTAAAAAAC
TTAAAAAGAACCTATCCTATGCTAATAATCATAACTTCAATAAAGTTATAATGATAGGTCAACAAGAAGTTGAAGATGAA
ACAGTAACAATTAAGGATATGGATTCTGGATGTCAGGAAACAATACCACAAAATACTGTTGTATCTGAATTACTAAAATA
G

Upstream 100 bases:

>100_bases
CATAAATAAAGAGTTATGTGTATGTATTCTAGTATAATTTAAAATAATTTAAAGGGACAAGTATGTACTTGTATAATTTA
ATAAAAGGAGTATAATAGTC

Downstream 100 bases:

>100_bases
GGATAAACATGATAAAACCAAACTTTAGACATATTATAAATGGTAAAAGATTAGCAACTGCCATAGCCCAAGATTATAAA
ACAGGTGAAATATTAATGGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MEFNKPRGTRDFLFEDMEERNYVENTIRNVVENYGFKEIKTPIFEDLELFTTKSGEGIKEEIYHFQDKGGRDLALRPELT
ASVSRLYNNNLQKSAKPLKMYYFGSCFRYERPQAGRFREFWQFGIEVIGGVPIYNEAEIIAMANEALTQVGIKNYEINIG
HLGIIKGVLNQLNISSQQQTAIIASIDKEDYELLDKLLEDNDISSEYKNIITSIINIKGSRSDLETLKQLLEDIPESFEA
LENLDKTLEVLEVFGFDDYVVNLSIARGLDYYTGIVFEIYVPDLGAEKQITGGGTYNLTALFDSEKVESTGFAFGFDRIM
EAYHRQNIQVPISSKPKVLVIPVKKEFKLNAIEVAQQLRAHNIVTDIDLKGKKLKKNLSYANNHNFNKVIMIGQQEVEDE
TVTIKDMDSGCQETIPQNTVVSELLK

Sequences:

>Translated_426_residues
MEFNKPRGTRDFLFEDMEERNYVENTIRNVVENYGFKEIKTPIFEDLELFTTKSGEGIKEEIYHFQDKGGRDLALRPELT
ASVSRLYNNNLQKSAKPLKMYYFGSCFRYERPQAGRFREFWQFGIEVIGGVPIYNEAEIIAMANEALTQVGIKNYEINIG
HLGIIKGVLNQLNISSQQQTAIIASIDKEDYELLDKLLEDNDISSEYKNIITSIINIKGSRSDLETLKQLLEDIPESFEA
LENLDKTLEVLEVFGFDDYVVNLSIARGLDYYTGIVFEIYVPDLGAEKQITGGGTYNLTALFDSEKVESTGFAFGFDRIM
EAYHRQNIQVPISSKPKVLVIPVKKEFKLNAIEVAQQLRAHNIVTDIDLKGKKLKKNLSYANNHNFNKVIMIGQQEVEDE
TVTIKDMDSGCQETIPQNTVVSELLK
>Mature_426_residues
MEFNKPRGTRDFLFEDMEERNYVENTIRNVVENYGFKEIKTPIFEDLELFTTKSGEGIKEEIYHFQDKGGRDLALRPELT
ASVSRLYNNNLQKSAKPLKMYYFGSCFRYERPQAGRFREFWQFGIEVIGGVPIYNEAEIIAMANEALTQVGIKNYEINIG
HLGIIKGVLNQLNISSQQQTAIIASIDKEDYELLDKLLEDNDISSEYKNIITSIINIKGSRSDLETLKQLLEDIPESFEA
LENLDKTLEVLEVFGFDDYVVNLSIARGLDYYTGIVFEIYVPDLGAEKQITGGGTYNLTALFDSEKVESTGFAFGFDRIM
EAYHRQNIQVPISSKPKVLVIPVKKEFKLNAIEVAQQLRAHNIVTDIDLKGKKLKKNLSYANNHNFNKVIMIGQQEVEDE
TVTIKDMDSGCQETIPQNTVVSELLK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=448, Percent_Identity=27.6785714285714, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI15029520, Length=448, Percent_Identity=27.4553571428571, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788861, Length=439, Percent_Identity=27.7904328018223, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI71993693, Length=455, Percent_Identity=27.4725274725275, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71993686, Length=455, Percent_Identity=27.4725274725275, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6325290, Length=377, Percent_Identity=27.8514588859416, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643061, Length=451, Percent_Identity=25.9423503325942, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24643059, Length=470, Percent_Identity=24.8936170212766, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48589; Mature: 48589

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNKPRGTRDFLFEDMEERNYVENTIRNVVENYGFKEIKTPIFEDLELFTTKSGEGIKE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCCCHHH
EIYHFQDKGGRDLALRPELTASVSRLYNNNLQKSAKPLKMYYFGSCFRYERPQAGRFREF
HHHHHHCCCCCCEEECCHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
WQFGIEVIGGVPIYNEAEIIAMANEALTQVGIKNYEINIGHLGIIKGVLNQLNISSQQQT
HHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCHHE
AIIASIDKEDYELLDKLLEDNDISSEYKNIITSIINIKGSRSDLETLKQLLEDIPESFEA
EEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LENLDKTLEVLEVFGFDDYVVNLSIARGLDYYTGIVFEIYVPDLGAEKQITGGGTYNLTA
HHHHHHHHHHHHHHCCCCEEEEEEHHHCCHHHHCEEEEEEECCCCCCCEECCCCCEEEEE
LFDSEKVESTGFAFGFDRIMEAYHRQNIQVPISSKPKVLVIPVKKEFKLNAIEVAQQLRA
EECCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHH
HNIVTDIDLKGKKLKKNLSYANNHNFNKVIMIGQQEVEDETVTIKDMDSGCQETIPQNTV
HCCEEECCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHHHCCCHHH
VSELLK
HHHHHC
>Mature Secondary Structure
MEFNKPRGTRDFLFEDMEERNYVENTIRNVVENYGFKEIKTPIFEDLELFTTKSGEGIKE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCCCHHH
EIYHFQDKGGRDLALRPELTASVSRLYNNNLQKSAKPLKMYYFGSCFRYERPQAGRFREF
HHHHHHCCCCCCEEECCHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
WQFGIEVIGGVPIYNEAEIIAMANEALTQVGIKNYEINIGHLGIIKGVLNQLNISSQQQT
HHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCHHE
AIIASIDKEDYELLDKLLEDNDISSEYKNIITSIINIKGSRSDLETLKQLLEDIPESFEA
EEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LENLDKTLEVLEVFGFDDYVVNLSIARGLDYYTGIVFEIYVPDLGAEKQITGGGTYNLTA
HHHHHHHHHHHHHHCCCCEEEEEEHHHCCHHHHCEEEEEEECCCCCCCEECCCCCEEEEE
LFDSEKVESTGFAFGFDRIMEAYHRQNIQVPISSKPKVLVIPVKKEFKLNAIEVAQQLRA
EECCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHH
HNIVTDIDLKGKKLKKNLSYANNHNFNKVIMIGQQEVEDETVTIKDMDSGCQETIPQNTV
HCCEEECCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHHHCCCHHH
VSELLK
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]