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The map label for this gene is pheS

Identifier: 84489241

GI number: 84489241

Start: 496183

End: 497736

Strand: Direct

Name: pheS

Synonym: Msp_0429

Alternate gene names: 84489241

Gene position: 496183-497736 (Clockwise)

Preceding gene: 84489240

Following gene: 84489242

Centisome position: 28.07

GC content: 31.98

Gene sequence:

>1554_bases
ATGATAGAAAAAATAATAAATGAATTACATCTTTATGAAAAAAAATTATTAGATGGTCTTGCAAAAAATTCTAATTTAAC
TCCAGAAGAAATAGCTAAATTAGAACAAATGCCTGTTAAAGCAGTAACAAGTGCGGCAGGAATGCTTGAGAGTAAAAATA
TTATCACAGTAGATAAAACATCAACAGATAATATAAGTTTATCTGAGGAAGGAAAAGACTATGCATTAAATGGACTTCCA
GAACACAGGATACTAAAAGCATTACAAGATTTTAGTAATGAAGGAAAAGATGAAGTGGGTATGGATGAAGTTATTGAAAG
GGCTAAAGTATCAAAGGCTCAGATGAACTTTTCAATTGGTATACTTATGCGTCATCATTGGGCTAAAATGAACAATGGAA
AATTAGCAATTACAGATGAAGGAAAAAATGCAGATATTTCAAGTCTACCTCAAGTTAAATTCCTTAAATTCCTACATGAT
AAGAAGCAAATCACTGAAGAAAACATACCTGAAGAATTTATTAAGGTTAGGGCTGAATTTAATAAGAGAAAAGATTTACT
TAATATTAAAACATCTCAAGACTTCCATTTTATACTTAATGATATTGGAAAGAAAATATTAGATGAAGGATTTTCCATAC
AAAATGAGGCAACACAACTAACACACGATCAACTAAAAACAGGTTCCTGGAAAAATCTACACTACAGGGGATATGATATC
AATGCATCAGCACCAAAGAATTATCCTGGAAAAATACATCCTCTTCAACAAACAATTGAAGAAATAAGAGATATTTTCAT
TGACATGGGTTTTGATGAGGCAAAGGGAACAATACTTGAATCAGCATTCTGGAACTTTGATATGTTATTCCAGCCACAAG
ATCATGCTGCACGTGAAATGCAAGATACATTCTATGTAAGTAATCCTTCCCAAGCATCACTGCCTGGTGGGGATTTAGTT
GAAAAAACTAGAGCTGAACATGAACATGGTGGAAATACTGGTAGTGAAGGATGGAATTATAAATGGAATGAGGATATAGC
AAAACAAATAGTTCTAAGAACACATACAACTGGTTTATCTGTACGTTATCTTTCCAAACATCAACCACCACTTAAAATGT
TTTCAGTAGGAAGAGTATTTAGACGTGAAACAATTAACTATAAACACTTACCTGAATTCCATCAAGTGGAAGGAATTGTT
GCAGGAGAAGATATGAGTTTTAAAAATCTACTTGGAATACTTAAAGAATTCTATAGAAAATTAGGTTTTAAAGTAAGATT
TAGACCAGCATACTTCCCATACACATACTTATCCATAGAATCTGAAATTTATGTTCCAGAAAAGAAAAGTTGGATGGAAC
TAGGTGGATCTGGAATGTTTAGACCAGAAGTACTAGAACCATTAGGAATAAAAACACAAGTAGCAGCATTTGGTCTTGGA
ATAGAAAGACTTGCAATGATGAGATATGGAATAGAAGATATTCGTATGTTATATCAAAGTGATATTGGATGGTTAAGAAA
ACTACCAGTAACAAGAAATATTAAAAACTTCTAA

Upstream 100 bases:

>100_bases
CTGGAACAACAGCTGATTTTACAGCAGCATCATTATTTGTAGGGTTAGTTGATAAGTATTCACAATCTGGTATATAACAG
TAGGGGAGGAATAATAATAA

Downstream 100 bases:

>100_bases
AAGGTGTTAATTATGGTATATAATTCTTTAGAATTAAATCTTGAAGCAATTACAAACACAATTAAAATGTTGGAAAAGGA
AAATAACGATGAAAATAAGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MIEKIINELHLYEKKLLDGLAKNSNLTPEEIAKLEQMPVKAVTSAAGMLESKNIITVDKTSTDNISLSEEGKDYALNGLP
EHRILKALQDFSNEGKDEVGMDEVIERAKVSKAQMNFSIGILMRHHWAKMNNGKLAITDEGKNADISSLPQVKFLKFLHD
KKQITEENIPEEFIKVRAEFNKRKDLLNIKTSQDFHFILNDIGKKILDEGFSIQNEATQLTHDQLKTGSWKNLHYRGYDI
NASAPKNYPGKIHPLQQTIEEIRDIFIDMGFDEAKGTILESAFWNFDMLFQPQDHAAREMQDTFYVSNPSQASLPGGDLV
EKTRAEHEHGGNTGSEGWNYKWNEDIAKQIVLRTHTTGLSVRYLSKHQPPLKMFSVGRVFRRETINYKHLPEFHQVEGIV
AGEDMSFKNLLGILKEFYRKLGFKVRFRPAYFPYTYLSIESEIYVPEKKSWMELGGSGMFRPEVLEPLGIKTQVAAFGLG
IERLAMMRYGIEDIRMLYQSDIGWLRKLPVTRNIKNF

Sequences:

>Translated_517_residues
MIEKIINELHLYEKKLLDGLAKNSNLTPEEIAKLEQMPVKAVTSAAGMLESKNIITVDKTSTDNISLSEEGKDYALNGLP
EHRILKALQDFSNEGKDEVGMDEVIERAKVSKAQMNFSIGILMRHHWAKMNNGKLAITDEGKNADISSLPQVKFLKFLHD
KKQITEENIPEEFIKVRAEFNKRKDLLNIKTSQDFHFILNDIGKKILDEGFSIQNEATQLTHDQLKTGSWKNLHYRGYDI
NASAPKNYPGKIHPLQQTIEEIRDIFIDMGFDEAKGTILESAFWNFDMLFQPQDHAAREMQDTFYVSNPSQASLPGGDLV
EKTRAEHEHGGNTGSEGWNYKWNEDIAKQIVLRTHTTGLSVRYLSKHQPPLKMFSVGRVFRRETINYKHLPEFHQVEGIV
AGEDMSFKNLLGILKEFYRKLGFKVRFRPAYFPYTYLSIESEIYVPEKKSWMELGGSGMFRPEVLEPLGIKTQVAAFGLG
IERLAMMRYGIEDIRMLYQSDIGWLRKLPVTRNIKNF
>Mature_517_residues
MIEKIINELHLYEKKLLDGLAKNSNLTPEEIAKLEQMPVKAVTSAAGMLESKNIITVDKTSTDNISLSEEGKDYALNGLP
EHRILKALQDFSNEGKDEVGMDEVIERAKVSKAQMNFSIGILMRHHWAKMNNGKLAITDEGKNADISSLPQVKFLKFLHD
KKQITEENIPEEFIKVRAEFNKRKDLLNIKTSQDFHFILNDIGKKILDEGFSIQNEATQLTHDQLKTGSWKNLHYRGYDI
NASAPKNYPGKIHPLQQTIEEIRDIFIDMGFDEAKGTILESAFWNFDMLFQPQDHAAREMQDTFYVSNPSQASLPGGDLV
EKTRAEHEHGGNTGSEGWNYKWNEDIAKQIVLRTHTTGLSVRYLSKHQPPLKMFSVGRVFRRETINYKHLPEFHQVEGIV
AGEDMSFKNLLGILKEFYRKLGFKVRFRPAYFPYTYLSIESEIYVPEKKSWMELGGSGMFRPEVLEPLGIKTQVAAFGLG
IERLAMMRYGIEDIRMLYQSDIGWLRKLPVTRNIKNF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=492, Percent_Identity=34.1463414634146, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1788007, Length=265, Percent_Identity=33.9622641509434, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17508957, Length=510, Percent_Identity=32.3529411764706, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI32563657, Length=510, Percent_Identity=32.3529411764706, Blast_Score=261, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI32566635, Length=284, Percent_Identity=27.4647887323944, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321087, Length=508, Percent_Identity=31.496062992126, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI18858079, Length=496, Percent_Identity=32.258064516129, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17137424, Length=279, Percent_Identity=24.7311827956989, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 59338; Mature: 59338

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKIINELHLYEKKLLDGLAKNSNLTPEEIAKLEQMPVKAVTSAAGMLESKNIITVDKT
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECC
STDNISLSEEGKDYALNGLPEHRILKALQDFSNEGKDEVGMDEVIERAKVSKAQMNFSIG
CCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEE
ILMRHHWAKMNNGKLAITDEGKNADISSLPQVKFLKFLHDKKQITEENIPEEFIKVRAEF
HHHHHHHHCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NKRKDLLNIKTSQDFHFILNDIGKKILDEGFSIQNEATQLTHDQLKTGSWKNLHYRGYDI
HHCHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEECEEC
NASAPKNYPGKIHPLQQTIEEIRDIFIDMGFDEAKGTILESAFWNFDMLFQPQDHAAREM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHCCCEEEECCCHHHHHHH
QDTFYVSNPSQASLPGGDLVEKTRAEHEHGGNTGSEGWNYKWNEDIAKQIVLRTHTTGLS
HHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCE
VRYLSKHQPPLKMFSVGRVFRRETINYKHLPEFHQVEGIVAGEDMSFKNLLGILKEFYRK
EEEHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHH
LGFKVRFRPAYFPYTYLSIESEIYVPEKKSWMELGGSGMFRPEVLEPLGIKTQVAAFGLG
CCCEEEEECCCCCEEEEEECCEEECCCCHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHCC
IERLAMMRYGIEDIRMLYQSDIGWLRKLPVTRNIKNF
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIEKIINELHLYEKKLLDGLAKNSNLTPEEIAKLEQMPVKAVTSAAGMLESKNIITVDKT
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECC
STDNISLSEEGKDYALNGLPEHRILKALQDFSNEGKDEVGMDEVIERAKVSKAQMNFSIG
CCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEE
ILMRHHWAKMNNGKLAITDEGKNADISSLPQVKFLKFLHDKKQITEENIPEEFIKVRAEF
HHHHHHHHCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NKRKDLLNIKTSQDFHFILNDIGKKILDEGFSIQNEATQLTHDQLKTGSWKNLHYRGYDI
HHCHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEECEEC
NASAPKNYPGKIHPLQQTIEEIRDIFIDMGFDEAKGTILESAFWNFDMLFQPQDHAAREM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHCCCEEEECCCHHHHHHH
QDTFYVSNPSQASLPGGDLVEKTRAEHEHGGNTGSEGWNYKWNEDIAKQIVLRTHTTGLS
HHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCE
VRYLSKHQPPLKMFSVGRVFRRETINYKHLPEFHQVEGIVAGEDMSFKNLLGILKEFYRK
EEEHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHH
LGFKVRFRPAYFPYTYLSIESEIYVPEKKSWMELGGSGMFRPEVLEPLGIKTQVAAFGLG
CCCEEEEECCCCCEEEEEECCEEECCCCHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHCC
IERLAMMRYGIEDIRMLYQSDIGWLRKLPVTRNIKNF
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 10575359 [H]