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The map label for this gene is hemB

Identifier: 84489228

GI number: 84489228

Start: 478510

End: 479475

Strand: Direct

Name: hemB

Synonym: Msp_0416

Alternate gene names: 84489228

Gene position: 478510-479475 (Clockwise)

Preceding gene: 84489227

Following gene: 84489229

Centisome position: 27.07

GC content: 32.4

Gene sequence:

>966_bases
ATGTTTCCAGTTACAAGAATGAGAAGAATGAGATCAGATGAAAGAATAAGAAGCATGTTTCGTGAAACAAGTGTAGATAT
TAGTGATTTTATATATCCACTATATATTAAAGAATCAACAGAAAATGGAAATCCTGAAGAAATAAGTACAATGCCCGGTC
AATACAGATATTCTGTTGATGATGCAGTAGATTTTGCAGGACTTCTTGAAGATGATGGTTTAACATCAGTAATACTCTTT
GGAATACCAGATTATAAAGATGAAAAAGCATCCAGTGCATATGATAAAAATGGTATAATTCAACAAACAATCAAAGCATT
AAAAGAACAAACAAACCTAGTTGTTTTAGGTGATGTTTGTATGTGTGAATATACAGATCATGGACATTGTGGTATAATAA
AAGATAACTATGTTCAAAACGATGAAACACTAAAATATCTATCAAAAATAGCAGTAAGCTATGCAGATGCAGGAGTAGAT
GTAGTAGCACCAAGTGATATGATGGATGGAAGAGTAGGTGCAATTCGTGAAGCACTAGATAAAAATGATTATATCAATAC
TCCAATATTTTCATATGCAGCAAAATATGCATCCACATACTATGCACCATTTAGGGATGCAGCAGATTCAACTCCAAGCT
TTGGTGATCGTAAATCCTATCAAATGGATCCAGCAAATTTCAATGAAGCAATTAGAGAAGTAGCATTAGATGTTCAAGAA
GGAACAGATGCTATTATTGTTAAACCAGCACTAGCATATTTAGATGTCTTACGTGAAGTAAAACAAACATTTAAAATGCC
AACAATAGCATACCAAGTAAGTGGTGAATATTCAATGATACAGGCAGGAATAGAAAAAGGATACATTACAGAAGATTTAC
TATATGAAACACTACTTAGTATAAAACGTGCTGGAGCAGATATGATTATAAGTTATTTTGTACCACAACTACTTGGTATA
GAATAG

Upstream 100 bases:

>100_bases
GACTTTTTATCACTACAAGACTAACTAAACTTAATAACAAATAAAATATATAATTTTAAATAATAACTTAAAAAATTAAT
TAATAAAAAGATGATTTAAA

Downstream 100 bases:

>100_bases
AACAATATCTTCTTTTTTTTTACTTTTTTTAGTACCTAAAAGATTTAATTTTAAAACAATTCCATTTTTAATTTTAAATG
GATTTAATACTAAAATCATA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MFPVTRMRRMRSDERIRSMFRETSVDISDFIYPLYIKESTENGNPEEISTMPGQYRYSVDDAVDFAGLLEDDGLTSVILF
GIPDYKDEKASSAYDKNGIIQQTIKALKEQTNLVVLGDVCMCEYTDHGHCGIIKDNYVQNDETLKYLSKIAVSYADAGVD
VVAPSDMMDGRVGAIREALDKNDYINTPIFSYAAKYASTYYAPFRDAADSTPSFGDRKSYQMDPANFNEAIREVALDVQE
GTDAIIVKPALAYLDVLREVKQTFKMPTIAYQVSGEYSMIQAGIEKGYITEDLLYETLLSIKRAGADMIISYFVPQLLGI
E

Sequences:

>Translated_321_residues
MFPVTRMRRMRSDERIRSMFRETSVDISDFIYPLYIKESTENGNPEEISTMPGQYRYSVDDAVDFAGLLEDDGLTSVILF
GIPDYKDEKASSAYDKNGIIQQTIKALKEQTNLVVLGDVCMCEYTDHGHCGIIKDNYVQNDETLKYLSKIAVSYADAGVD
VVAPSDMMDGRVGAIREALDKNDYINTPIFSYAAKYASTYYAPFRDAADSTPSFGDRKSYQMDPANFNEAIREVALDVQE
GTDAIIVKPALAYLDVLREVKQTFKMPTIAYQVSGEYSMIQAGIEKGYITEDLLYETLLSIKRAGADMIISYFVPQLLGI
E
>Mature_321_residues
MFPVTRMRRMRSDERIRSMFRETSVDISDFIYPLYIKESTENGNPEEISTMPGQYRYSVDDAVDFAGLLEDDGLTSVILF
GIPDYKDEKASSAYDKNGIIQQTIKALKEQTNLVVLGDVCMCEYTDHGHCGIIKDNYVQNDETLKYLSKIAVSYADAGVD
VVAPSDMMDGRVGAIREALDKNDYINTPIFSYAAKYASTYYAPFRDAADSTPSFGDRKSYQMDPANFNEAIREVALDVQE
GTDAIIVKPALAYLDVLREVKQTFKMPTIAYQVSGEYSMIQAGIEKGYITEDLLYETLLSIKRAGADMIISYFVPQLLGI
E

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=303, Percent_Identity=42.2442244224422, Blast_Score=252, Evalue=3e-67,
Organism=Escherichia coli, GI87081728, Length=316, Percent_Identity=43.9873417721519, Blast_Score=261, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321398, Length=311, Percent_Identity=41.8006430868167, Blast_Score=228, Evalue=7e-61,
Organism=Drosophila melanogaster, GI21358291, Length=308, Percent_Identity=38.961038961039, Blast_Score=234, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36035; Mature: 36035

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPVTRMRRMRSDERIRSMFRETSVDISDFIYPLYIKESTENGNPEEISTMPGQYRYSVD
CCCHHHHHHHCCHHHHHHHHHHHCCCHHHCEEEEEEEECCCCCCCHHHHCCCCCEEECHH
DAVDFAGLLEDDGLTSVILFGIPDYKDEKASSAYDKNGIIQQTIKALKEQTNLVVLGDVC
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCEEEECCEE
MCEYTDHGHCGIIKDNYVQNDETLKYLSKIAVSYADAGVDVVAPSDMMDGRVGAIREALD
EEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHCCHHHHHHHHHC
KNDYINTPIFSYAAKYASTYYAPFRDAADSTPSFGDRKSYQMDPANFNEAIREVALDVQE
CCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GTDAIIVKPALAYLDVLREVKQTFKMPTIAYQVSGEYSMIQAGIEKGYITEDLLYETLLS
CCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
IKRAGADMIISYFVPQLLGIE
HHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFPVTRMRRMRSDERIRSMFRETSVDISDFIYPLYIKESTENGNPEEISTMPGQYRYSVD
CCCHHHHHHHCCHHHHHHHHHHHCCCHHHCEEEEEEEECCCCCCCHHHHCCCCCEEECHH
DAVDFAGLLEDDGLTSVILFGIPDYKDEKASSAYDKNGIIQQTIKALKEQTNLVVLGDVC
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCEEEECCEE
MCEYTDHGHCGIIKDNYVQNDETLKYLSKIAVSYADAGVDVVAPSDMMDGRVGAIREALD
EEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHCCHHHHHHHHHC
KNDYINTPIFSYAAKYASTYYAPFRDAADSTPSFGDRKSYQMDPANFNEAIREVALDVQE
CCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GTDAIIVKPALAYLDVLREVKQTFKMPTIAYQVSGEYSMIQAGIEKGYITEDLLYETLLS
CCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
IKRAGADMIISYFVPQLLGIE
HHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]