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The map label for this gene is speB [C]

Identifier: 84489227

GI number: 84489227

Start: 477546

End: 478433

Strand: Direct

Name: speB [C]

Synonym: Msp_0415

Alternate gene names: 84489227

Gene position: 477546-478433 (Clockwise)

Preceding gene: 84489226

Following gene: 84489228

Centisome position: 27.02

GC content: 30.97

Gene sequence:

>888_bases
ATGTTTTTTTATGCAGATAATATGATGAATTTTGCTTTTTCAAAATCTATAGATAATGAAGAGGAAATAGAGGAAGGTTA
TGCTATTATGGGTGTTGGATTTGACAGTACAACAAGTTACATGGCAGGTTCAAGGTATGGTCCAAAGGCTGTTAGAGAAG
CTTCATATAATTTTGAATCATATAATTTATCATTTGATACTTCACTAACTGCTTGTAGTTATGATATTGGAGATGTGTTT
GTAAATACAGGAAATTATGAAACAACACATGTTATGATTAAAGATACTGTAAAATCCATCCTTGAGATGGATTTGTATCC
TATTGTAATAGGGGGAGAACACACAATTACAAATGGTGTTCTTGGTGGTATATATGATTTTAATGAGGATCTTTTTCATA
ATTTAACAGTAGTGCATTTTGATGCTCATTTTGATATGAGAGATACATATCTTGATGAGAAATATTCACATGCAACAGTA
CTTAGAAGAATCCATGAACACAAACCCCAACAAATAATACAACTAGGTATACGATCAGCACAAAAAGAAGAATATGAATA
TGTAAAACAACAAGATAACATATCATACTATACAAATCATGATATTAAAGAAAATAAGAACGAAATACTAAAAGTATTAG
AAAAAATAGATACACCCATTTATATTACAGTAGATATTGATGTCCTAGATCCAGCATATGCACCCTCTGTAGGAACACCA
GCACCATGTGGAATTACACCATATGATTTGGAGGATATGATTGGAGTACTTTCAAAAAAGAATGTAGTGGGTATTGATGT
GGTTGAAGTTTCATCAAATACAATAGGAGATACAACAAGTATAAATGCAGCAAAGGTAATCTATGACTTTTTATCACTAC
AAGACTAA

Upstream 100 bases:

>100_bases
TTTTTTGTTGATTATAGTATACATTCCATAAAATTATTAGAATTAGATAATATACATATCTACTATAATAATTTAAAAGA
AATTAGGATGTGGTAATTCA

Downstream 100 bases:

>100_bases
CTAAACTTAATAACAAATAAAATATATAATTTTAAATAATAACTTAAAAAATTAATTAATAAAAAGATGATTTAAAATGT
TTCCAGTTACAAGAATGAGA

Product: arginase/agmatinase/formimionoglutamate hydrolase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MFFYADNMMNFAFSKSIDNEEEIEEGYAIMGVGFDSTTSYMAGSRYGPKAVREASYNFESYNLSFDTSLTACSYDIGDVF
VNTGNYETTHVMIKDTVKSILEMDLYPIVIGGEHTITNGVLGGIYDFNEDLFHNLTVVHFDAHFDMRDTYLDEKYSHATV
LRRIHEHKPQQIIQLGIRSAQKEEYEYVKQQDNISYYTNHDIKENKNEILKVLEKIDTPIYITVDIDVLDPAYAPSVGTP
APCGITPYDLEDMIGVLSKKNVVGIDVVEVSSNTIGDTTSINAAKVIYDFLSLQD

Sequences:

>Translated_295_residues
MFFYADNMMNFAFSKSIDNEEEIEEGYAIMGVGFDSTTSYMAGSRYGPKAVREASYNFESYNLSFDTSLTACSYDIGDVF
VNTGNYETTHVMIKDTVKSILEMDLYPIVIGGEHTITNGVLGGIYDFNEDLFHNLTVVHFDAHFDMRDTYLDEKYSHATV
LRRIHEHKPQQIIQLGIRSAQKEEYEYVKQQDNISYYTNHDIKENKNEILKVLEKIDTPIYITVDIDVLDPAYAPSVGTP
APCGITPYDLEDMIGVLSKKNVVGIDVVEVSSNTIGDTTSINAAKVIYDFLSLQD
>Mature_295_residues
MFFYADNMMNFAFSKSIDNEEEIEEGYAIMGVGFDSTTSYMAGSRYGPKAVREASYNFESYNLSFDTSLTACSYDIGDVF
VNTGNYETTHVMIKDTVKSILEMDLYPIVIGGEHTITNGVLGGIYDFNEDLFHNLTVVHFDAHFDMRDTYLDEKYSHATV
LRRIHEHKPQQIIQLGIRSAQKEEYEYVKQQDNISYYTNHDIKENKNEILKVLEKIDTPIYITVDIDVLDPAYAPSVGTP
APCGITPYDLEDMIGVLSKKNVVGIDVVEVSSNTIGDTTSINAAKVIYDFLSLQD

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=278, Percent_Identity=32.0143884892086, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1789306, Length=258, Percent_Identity=29.8449612403101, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 33308; Mature: 33308

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFYADNMMNFAFSKSIDNEEEIEEGYAIMGVGFDSTTSYMAGSRYGPKAVREASYNFES
CEEEECCHHHHHHHCCCCCHHHHHCCEEEEEECCCCCHHHHCCCCCCHHHHHHHCCCCEE
YNLSFDTSLTACSYDIGDVFVNTGNYETTHVMIKDTVKSILEMDLYPIVIGGEHTITNGV
EEEEECCCCEEEEEECCEEEEECCCCEEEEEEHHHHHHHHHHCCCEEEEECCCEEECCCC
LGGIYDFNEDLFHNLTVVHFDAHFDMRDTYLDEKYSHATVLRRIHEHKPQQIIQLGIRSA
CCCHHHCCHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QKEEYEYVKQQDNISYYTNHDIKENKNEILKVLEKIDTPIYITVDIDVLDPAYAPSVGTP
HHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCC
APCGITPYDLEDMIGVLSKKNVVGIDVVEVSSNTIGDTTSINAAKVIYDFLSLQD
CCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFFYADNMMNFAFSKSIDNEEEIEEGYAIMGVGFDSTTSYMAGSRYGPKAVREASYNFES
CEEEECCHHHHHHHCCCCCHHHHHCCEEEEEECCCCCHHHHCCCCCCHHHHHHHCCCCEE
YNLSFDTSLTACSYDIGDVFVNTGNYETTHVMIKDTVKSILEMDLYPIVIGGEHTITNGV
EEEEECCCCEEEEEECCEEEEECCCCEEEEEEHHHHHHHHHHCCCEEEEECCCEEECCCC
LGGIYDFNEDLFHNLTVVHFDAHFDMRDTYLDEKYSHATVLRRIHEHKPQQIIQLGIRSA
CCCHHHCCHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QKEEYEYVKQQDNISYYTNHDIKENKNEILKVLEKIDTPIYITVDIDVLDPAYAPSVGTP
HHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCC
APCGITPYDLEDMIGVLSKKNVVGIDVVEVSSNTIGDTTSINAAKVIYDFLSLQD
CCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2377617 [H]