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The map label for this gene is leuD

Identifier: 84489188

GI number: 84489188

Start: 441526

End: 442017

Strand: Direct

Name: leuD

Synonym: Msp_0374

Alternate gene names: 84489188

Gene position: 441526-442017 (Clockwise)

Preceding gene: 84489187

Following gene: 84489189

Centisome position: 24.98

GC content: 30.89

Gene sequence:

>492_bases
ATGGATAGTATGAAAGGAAAAGTATGGACATTTAGAGATTGTATAGATACTGATGTAATTATTGCTGGAAGATATTTAAG
AACATTTAATCCAGAAGATTTAGCAGCACATGTAATGGAAGCTGAAGATCCAGAATTTTCAAGTAAAGTTGGAAAGGGAG
ATATAATTGTTGGTGGATGGAATTTTGGTTGTGGATCTTCAAGGGAACAGGCACCAGTTGCAATAAAGACAGCAGGAGTA
TCAGCAGTAATAGCTAAATCATTTGCCAGAATTTTTTATAGAAATGCTATTAATATTGGCTTACCTGTTATAACAGCAGA
TATTGAAGTGGATGAAGGGGATATTTTAGAAGTAAATATTGAAGATGGAATTATTATAAATGAAACAACTAAAAAAACAT
TTAAAATTAAGCCATTTGATGCTGAAATGTTGGATATTTTAGAAAATGGTGGTCTTGTTAATCAATATTTAAAAAATAAG
AAGGAGGTATAG

Upstream 100 bases:

>100_bases
GTATGAAAAAAGATCTTCAGGATTTAATGGGTAATGATAGTTTTATAAAAATTACAAATGGAAAAAATGAAGGTTCAAAA
ATAAGATAGGAGGAAGATTT

Downstream 100 bases:

>100_bases
TTATGGTTTGTCCAGTTTGTGGTGAAGATGAATATGAAATATTAAAGGCTAATGGAAAAAATAATAGACAATTACTAGTT
AAATGTGATGAATGTGGACA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MDSMKGKVWTFRDCIDTDVIIAGRYLRTFNPEDLAAHVMEAEDPEFSSKVGKGDIIVGGWNFGCGSSREQAPVAIKTAGV
SAVIAKSFARIFYRNAINIGLPVITADIEVDEGDILEVNIEDGIIINETTKKTFKIKPFDAEMLDILENGGLVNQYLKNK
KEV

Sequences:

>Translated_163_residues
MDSMKGKVWTFRDCIDTDVIIAGRYLRTFNPEDLAAHVMEAEDPEFSSKVGKGDIIVGGWNFGCGSSREQAPVAIKTAGV
SAVIAKSFARIFYRNAINIGLPVITADIEVDEGDILEVNIEDGIIINETTKKTFKIKPFDAEMLDILENGGLVNQYLKNK
KEV
>Mature_163_residues
MDSMKGKVWTFRDCIDTDVIIAGRYLRTFNPEDLAAHVMEAEDPEFSSKVGKGDIIVGGWNFGCGSSREQAPVAIKTAGV
SAVIAKSFARIFYRNAINIGLPVITADIEVDEGDILEVNIEDGIIINETTKKTFKIKPFDAEMLDILENGGLVNQYLKNK
KEV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=112, Percent_Identity=38.3928571428571, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=91, Percent_Identity=47.2527472527472, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 17958; Mature: 17958

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSMKGKVWTFRDCIDTDVIIAGRYLRTFNPEDLAAHVMEAEDPEFSSKVGKGDIIVGGW
CCCCCCCEEEEHHHCCCCEEEECCEEECCCHHHHHHHHHCCCCCCCHHCCCCCCEEEECC
NFGCGSSREQAPVAIKTAGVSAVIAKSFARIFYRNAINIGLPVITADIEVDEGDILEVNI
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEE
EDGIIINETTKKTFKIKPFDAEMLDILENGGLVNQYLKNKKEV
CCCEEEECCCCCEEEECCCCHHHHHHHHCCCHHHHHHHCCCCC
>Mature Secondary Structure
MDSMKGKVWTFRDCIDTDVIIAGRYLRTFNPEDLAAHVMEAEDPEFSSKVGKGDIIVGGW
CCCCCCCEEEEHHHCCCCEEEECCEEECCCHHHHHHHHHCCCCCCCHHCCCCCCEEEECC
NFGCGSSREQAPVAIKTAGVSAVIAKSFARIFYRNAINIGLPVITADIEVDEGDILEVNI
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEE
EDGIIINETTKKTFKIKPFDAEMLDILENGGLVNQYLKNKKEV
CCCEEEECCCCCEEEECCCCHHHHHHHHCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]