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The map label for this gene is wcaJ [H]

Identifier: 84489036

GI number: 84489036

Start: 255221

End: 256609

Strand: Direct

Name: wcaJ [H]

Synonym: Msp_0208

Alternate gene names: 84489036

Gene position: 255221-256609 (Clockwise)

Preceding gene: 84489035

Following gene: 84489037

Centisome position: 14.44

GC content: 27.29

Gene sequence:

>1389_bases
ATGATACGAGAAAATCAAAATGTTTTTAATATATGTAATGTTTTACTAGATATTCTAGTATTGGTTATATCAGTGTTTTT
CTTGTATCATACATCAATGATACAACATAGGTATCCAATATCATTTTATTATACAACACTACTTTCTCTATTTATATTAT
TAATACCTAGTTATCTGTTCTTATATTATGAATTTAAATTATACACACCTCAAAGAACAAATAGAAGTATTTTTTCAGAA
TCATCAAAAATTTTACAAGTAAATTTCTTTGAATATTTATTTTTATCAGCAATGTGTTATATGGAATTTTTAAATGTTTC
AGCAGATTTTCTAATTAGTTTTTTAGTAATTAATTCTATTTTAGCAGTAATAGAACGCTCTATTATTAGAGGATTTCTAA
GATTACTTAGAAGTAAGGGTTTTAATATAAAATATGTTCTAGTTATTGGTGCAGGAGAAGTTGGAAGAAATCTTGTAGAA
ACTATAAAAATTAACACATACTTAGGATATAAAGTTATAGGCTTTCTTGATGATAATGTAATAGGTACTGTTAATGATGT
TAAGGTAATTGGAAAAATAAATACTTTAGAATCGATACTAGATACACACATGATTGATAGAGTTATTGTAAGTATTGCAC
CACGTCATTATAAGACATTAGAATCTATAATGGCTTGTTGTGAAAAGATGGGTGTCCGTGCAGATATAGTTCCAGATTAT
TATAGGTTTATAACATCTCATCCTACTATTGAGCTCTTGGATAACATTCCATTAATTAGTGTTCGTTATCTACCCCTTGA
TATTTCAATTAACAAGTACTTAAAAAGAACATTTGATGTTTTATTCGTTGTTGTAGCATCAATAATATTATCTCCACTTC
TTGTGATTGTTGCAATAGCAATAAAACTAACATCTCCAGGTCCAATTATATATAAACAAGAACGTGTAGGTGAGAATGCA
AAACCATTTATGATATATAAATTTAGAAGTATGAAATCTGAAAATGAATATATTGATGATAAAAAATGGACACAGAAAGA
TGATCCAAGAGTAACAACTATTGGTAAAATCATTAGAAAAACAAGTATTGATGAATTACCACAATTCTATAATATTTTCA
AGGGGGATATGAGTCTTATTGGACCAAGACCAGAAAGACCATATTTTGTAAATAAATTCAGAGAATCAGTACCTAAATAC
ATGATAAAGCATCATGTTAAGCCGGGTATGACTGGATGGGCACAGGTTAATGGTTATAGGGGTAATACATCTATTGTAAA
GAGAATTAAATATGATATTTATTATGTTGAAAACTGGTCTATAATGTTGGATATTCGTATCTTTTTTAAAACATTACCTG
CTCTATTAAATGATAAAAACGCATACTAA

Upstream 100 bases:

>100_bases
ATAAAACAACACATATAGTACTTACTTTTTTTTTATTTATTTTTTGAATAATTTTTATTATCCATAAAAAACATATATTA
AAATAATAGGAGTTTGTGTT

Downstream 100 bases:

>100_bases
AGAAGTTGATATAATTATGAATATAAATTTTAATAAAAAGAAATATTTGCTAATTCCATTAATACTAGCAGCCATACTTT
CTTTATCTGTATTATTTAGT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MIRENQNVFNICNVLLDILVLVISVFFLYHTSMIQHRYPISFYYTTLLSLFILLIPSYLFLYYEFKLYTPQRTNRSIFSE
SSKILQVNFFEYLFLSAMCYMEFLNVSADFLISFLVINSILAVIERSIIRGFLRLLRSKGFNIKYVLVIGAGEVGRNLVE
TIKINTYLGYKVIGFLDDNVIGTVNDVKVIGKINTLESILDTHMIDRVIVSIAPRHYKTLESIMACCEKMGVRADIVPDY
YRFITSHPTIELLDNIPLISVRYLPLDISINKYLKRTFDVLFVVVASIILSPLLVIVAIAIKLTSPGPIIYKQERVGENA
KPFMIYKFRSMKSENEYIDDKKWTQKDDPRVTTIGKIIRKTSIDELPQFYNIFKGDMSLIGPRPERPYFVNKFRESVPKY
MIKHHVKPGMTGWAQVNGYRGNTSIVKRIKYDIYYVENWSIMLDIRIFFKTLPALLNDKNAY

Sequences:

>Translated_462_residues
MIRENQNVFNICNVLLDILVLVISVFFLYHTSMIQHRYPISFYYTTLLSLFILLIPSYLFLYYEFKLYTPQRTNRSIFSE
SSKILQVNFFEYLFLSAMCYMEFLNVSADFLISFLVINSILAVIERSIIRGFLRLLRSKGFNIKYVLVIGAGEVGRNLVE
TIKINTYLGYKVIGFLDDNVIGTVNDVKVIGKINTLESILDTHMIDRVIVSIAPRHYKTLESIMACCEKMGVRADIVPDY
YRFITSHPTIELLDNIPLISVRYLPLDISINKYLKRTFDVLFVVVASIILSPLLVIVAIAIKLTSPGPIIYKQERVGENA
KPFMIYKFRSMKSENEYIDDKKWTQKDDPRVTTIGKIIRKTSIDELPQFYNIFKGDMSLIGPRPERPYFVNKFRESVPKY
MIKHHVKPGMTGWAQVNGYRGNTSIVKRIKYDIYYVENWSIMLDIRIFFKTLPALLNDKNAY
>Mature_462_residues
MIRENQNVFNICNVLLDILVLVISVFFLYHTSMIQHRYPISFYYTTLLSLFILLIPSYLFLYYEFKLYTPQRTNRSIFSE
SSKILQVNFFEYLFLSAMCYMEFLNVSADFLISFLVINSILAVIERSIIRGFLRLLRSKGFNIKYVLVIGAGEVGRNLVE
TIKINTYLGYKVIGFLDDNVIGTVNDVKVIGKINTLESILDTHMIDRVIVSIAPRHYKTLESIMACCEKMGVRADIVPDY
YRFITSHPTIELLDNIPLISVRYLPLDISINKYLKRTFDVLFVVVASIILSPLLVIVAIAIKLTSPGPIIYKQERVGENA
KPFMIYKFRSMKSENEYIDDKKWTQKDDPRVTTIGKIIRKTSIDELPQFYNIFKGDMSLIGPRPERPYFVNKFRESVPKY
MIKHHVKPGMTGWAQVNGYRGNTSIVKRIKYDIYYVENWSIMLDIRIFFKTLPALLNDKNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=466, Percent_Identity=30.0429184549356, Blast_Score=238, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53758; Mature: 53758

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRENQNVFNICNVLLDILVLVISVFFLYHTSMIQHRYPISFYYTTLLSLFILLIPSYLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LYYEFKLYTPQRTNRSIFSESSKILQVNFFEYLFLSAMCYMEFLNVSADFLISFLVINSI
HHHEEEEECCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAVIERSIIRGFLRLLRSKGFNIKYVLVIGAGEVGRNLVETIKINTYLGYKVIGFLDDNV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHEEEEEECEEEEEEECCCC
IGTVNDVKVIGKINTLESILDTHMIDRVIVSIAPRHYKTLESIMACCEKMGVRADIVPDY
EECCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHH
YRFITSHPTIELLDNIPLISVRYLPLDISINKYLKRTFDVLFVVVASIILSPLLVIVAIA
HHHHHCCCCHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLTSPGPIIYKQERVGENAKPFMIYKFRSMKSENEYIDDKKWTQKDDPRVTTIGKIIRK
HHCCCCCCEEEEHHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TSIDELPQFYNIFKGDMSLIGPRPERPYFVNKFRESVPKYMIKHHVKPGMTGWAQVNGYR
CCHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCC
GNTSIVKRIKYDIYYVENWSIMLDIRIFFKTLPALLNDKNAY
CCHHHHHHHEEEEEEEECCEEEEHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIRENQNVFNICNVLLDILVLVISVFFLYHTSMIQHRYPISFYYTTLLSLFILLIPSYLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LYYEFKLYTPQRTNRSIFSESSKILQVNFFEYLFLSAMCYMEFLNVSADFLISFLVINSI
HHHEEEEECCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAVIERSIIRGFLRLLRSKGFNIKYVLVIGAGEVGRNLVETIKINTYLGYKVIGFLDDNV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHEEEEEECEEEEEEECCCC
IGTVNDVKVIGKINTLESILDTHMIDRVIVSIAPRHYKTLESIMACCEKMGVRADIVPDY
EECCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHH
YRFITSHPTIELLDNIPLISVRYLPLDISINKYLKRTFDVLFVVVASIILSPLLVIVAIA
HHHHHCCCCHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLTSPGPIIYKQERVGENAKPFMIYKFRSMKSENEYIDDKKWTQKDDPRVTTIGKIIRK
HHCCCCCCEEEEHHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TSIDELPQFYNIFKGDMSLIGPRPERPYFVNKFRESVPKYMIKHHVKPGMTGWAQVNGYR
CCHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCC
GNTSIVKRIKYDIYYVENWSIMLDIRIFFKTLPALLNDKNAY
CCHHHHHHHEEEEEEEECCEEEEHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]