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The map label for this gene is epsB [H]

Identifier: 83721637

GI number: 83721637

Start: 1531254

End: 1533494

Strand: Direct

Name: epsB [H]

Synonym: BTH_I1354

Alternate gene names: 83721637

Gene position: 1531254-1533494 (Clockwise)

Preceding gene: 83721645

Following gene: 83720582

Centisome position: 40.2

GC content: 66.09

Gene sequence:

>2241_bases
ATGAATCCCAATCCAACCGGCACATCGGCGCTCGCCGACAGCGAAGGCGACACCGATTTCGTCGCCGTTCTCGACATCCT
GATCGAAGGCCGCTGGCTGATCGCGGCGATCGCGCTCGGCTGCTTCGTCGTGGGCGTCGCGTATGCGGTGCTCAGCAAGC
CCGTCTATCAGGCCGACATCCTGATCCAGGTCGAGGACAGCCCCGATACGTCAGCGGCGAAGAGTCTGCTCGGCGACGTG
TCTTCGCTCTTCGACGTGAAGTCGTCCGCGGCGGCCGAAACGCAGATTCTCGCGTCGCGGCTCGTCGTGTCGCGCGCCGT
CGACAATCTGAAGCTCTTCATCGACGCGAAGCCGAAGCGCTTTCCGGTGATCGGCAATTGGCTCGCGCGCCGCAGCGAAG
GGCTGTCGAATCCGGGGCTCGCGGGCTTCGGCGGCTACGCGTGGGGCCAGGAGCGCATCAACGTCGCGACGTTCGACGTG
CCGCGCGCGATGGAAGGCGACACGTTCGAGCTGACGATGCTCGATGCGCGCCGCTATCGGCTCGCCGGCGGCGATCTCGA
ACGCAATGTCGAGGGTGCGATCGGCACGCTCGAACGCTTTTCGGCGAAGGGCGGGGCGATCGTGTTGCGCGTCGACGCGG
TTGCCGCGAAGCCGGGCGCGACGTTTGTGCTCGTACGCCATTCGCGTTTGCGAACGATCGAGGATCTGCAGGACAACCTC
GATGTGCAGGAGCGCGTCAAGCAGTCCGACGTCGTCGTCGCGAGCCTGCGCGACACTGACCCCGACCTCGTCAGCAGCGC
GCTCAATGAAATCGGGCGGCAGTACATCGCGCAGAACATTCAGCGCAAATCGGCGGAAGCCGCGCAATCGCTCGAGTTCC
TGAACGGACAGCTGCCGGCGCTCAAGCGGCAGTTGACCGATTCCGAGGCGCGGCTCACGAAGCTGCGCGACGAGCACGGC
ACCGTCGATCTGACCGAAGAGGCGAAGCTCGCGCTCGCGCAGTCGGCCGATGCGAAGACGCGTCTGCTCGAATTGCGGCA
AAAACGGCAGGAGGCGCTGTCGCGCTTCACGCCGAAGCATCCGAGCGTCATCGCAATCGATCAGCAGATCGCCGCGCTCG
ACGGCTATCGCGGTGCGGCCGAGCAGCAGATCAAGCGGTTGCCGGATCTGCAGCAGCAGCTCGTGCGGCTGATGCTCGAC
GTGAAGGTCAATACCGATCTGTATACGGCGCTGCTGAACAACATGCAGCAGTTGCAGCTCGTGCGCGCGGGCAAGGTCGG
CAACGTGCGGCTCGTCGATACGGCGGCGGTGCCGGAAGTGCCCGTCAAGCCGAAGAAGGCGCTCGTCGCGCTCGCGTCGC
TGCTGCTCGGCGTGCTCGCCGGTTGCGGCACGGCGGTCGGCCGCTCGATGCTGTTCCATGGCATTTCCGATCCGAACGAG
ATCGAGCGCCGTCTCGGCCTGAACGTCTATGCGACCGTGCCGCGCAGCGATCAGCAGCGGGCGCTGACCGAGCGCGCGAA
GCGCAGGGAGCGCGCCCTGTCGCTGCTGTCCGTCGCGCATCCGGACGAGCCGGCCGTCGAAAGCCTGCGCAGCCTGCGCA
CCGCGCTGCAGTTCGCGATGCTCGACGCGAGGAACAACGTCGTCGTGATCGCGGGGCCCGCGCCGGGTGTCGGCAAGTCG
TTCGTGTCGGCGAATCTCGCCGCGGTGCTGACGATGGCGGGCAAGCGCGTGCTGCTGATCGACGGCGACATTCGCAAGGG
ACATCTGAACGACTATCTCGGCCTCGCTCGCGGCAAGGGCTTTTCGGAGCTGATCGCGGGATCGGCGCAGCCGGACGAGG
TGCTGCACCGCGATGTGATCGTCGGACTCGATTTCATTTCGACGGGCGCGATGCCGAAGCATCCCGCCGAGCTGTTGCTC
CATCCGCGCTTGCCCGAATTGATCGGCGAATTCTCGAAGCATTACGACGTTGTCCTGATCGATTCGCCGCCGGTGCTTGC
GGTGGCCGACACGGGCATTCTCGCCGCGACGGCGGGCACCGCGTTCCTCGTCGCGCTCGCCGGCTCGACGAAGCTCGGCG
AGATCGCGGAATCCGCGAAGCGGCTCGCGCAGAACGGCGTGCGTCTGAGCGGCGTCGTGTTCAACGGCATCAATCCGCGG
CTCGGGCAGTACGGCTATGGCTCGAAGTACGGCGGCTATCGCTACGTCGCGTACGAATACGGCGCGAAGCACGATGCGTG
A

Upstream 100 bases:

>100_bases
CCGCGCGGCTCCTAGAGCTTGGCGTGAACGGCTGGCTGGCGCGGCTGCGGCTCGTGTGAGCGCGCGCGGCGCGTAGAACC
GAATCTTCGAGACCCCACAG

Downstream 100 bases:

>100_bases
CGCACACCTCGGGGCGGGCGGACGCGACGGCAGGCGCCGGGTCATGCCGGAGGGCGAGCGATGCGCCGGCGCGCATGCGG
AGGCAAGGCGATGATTCGCG

Product: exopolysaccharide tyrosine-protein kinase

Products: ADP; protein tyrosine phosphate [C]

Alternate protein names: EPS I polysaccharide export protein epsB [H]

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MNPNPTGTSALADSEGDTDFVAVLDILIEGRWLIAAIALGCFVVGVAYAVLSKPVYQADILIQVEDSPDTSAAKSLLGDV
SSLFDVKSSAAAETQILASRLVVSRAVDNLKLFIDAKPKRFPVIGNWLARRSEGLSNPGLAGFGGYAWGQERINVATFDV
PRAMEGDTFELTMLDARRYRLAGGDLERNVEGAIGTLERFSAKGGAIVLRVDAVAAKPGATFVLVRHSRLRTIEDLQDNL
DVQERVKQSDVVVASLRDTDPDLVSSALNEIGRQYIAQNIQRKSAEAAQSLEFLNGQLPALKRQLTDSEARLTKLRDEHG
TVDLTEEAKLALAQSADAKTRLLELRQKRQEALSRFTPKHPSVIAIDQQIAALDGYRGAAEQQIKRLPDLQQQLVRLMLD
VKVNTDLYTALLNNMQQLQLVRAGKVGNVRLVDTAAVPEVPVKPKKALVALASLLLGVLAGCGTAVGRSMLFHGISDPNE
IERRLGLNVYATVPRSDQQRALTERAKRRERALSLLSVAHPDEPAVESLRSLRTALQFAMLDARNNVVVIAGPAPGVGKS
FVSANLAAVLTMAGKRVLLIDGDIRKGHLNDYLGLARGKGFSELIAGSAQPDEVLHRDVIVGLDFISTGAMPKHPAELLL
HPRLPELIGEFSKHYDVVLIDSPPVLAVADTGILAATAGTAFLVALAGSTKLGEIAESAKRLAQNGVRLSGVVFNGINPR
LGQYGYGSKYGGYRYVAYEYGAKHDA

Sequences:

>Translated_746_residues
MNPNPTGTSALADSEGDTDFVAVLDILIEGRWLIAAIALGCFVVGVAYAVLSKPVYQADILIQVEDSPDTSAAKSLLGDV
SSLFDVKSSAAAETQILASRLVVSRAVDNLKLFIDAKPKRFPVIGNWLARRSEGLSNPGLAGFGGYAWGQERINVATFDV
PRAMEGDTFELTMLDARRYRLAGGDLERNVEGAIGTLERFSAKGGAIVLRVDAVAAKPGATFVLVRHSRLRTIEDLQDNL
DVQERVKQSDVVVASLRDTDPDLVSSALNEIGRQYIAQNIQRKSAEAAQSLEFLNGQLPALKRQLTDSEARLTKLRDEHG
TVDLTEEAKLALAQSADAKTRLLELRQKRQEALSRFTPKHPSVIAIDQQIAALDGYRGAAEQQIKRLPDLQQQLVRLMLD
VKVNTDLYTALLNNMQQLQLVRAGKVGNVRLVDTAAVPEVPVKPKKALVALASLLLGVLAGCGTAVGRSMLFHGISDPNE
IERRLGLNVYATVPRSDQQRALTERAKRRERALSLLSVAHPDEPAVESLRSLRTALQFAMLDARNNVVVIAGPAPGVGKS
FVSANLAAVLTMAGKRVLLIDGDIRKGHLNDYLGLARGKGFSELIAGSAQPDEVLHRDVIVGLDFISTGAMPKHPAELLL
HPRLPELIGEFSKHYDVVLIDSPPVLAVADTGILAATAGTAFLVALAGSTKLGEIAESAKRLAQNGVRLSGVVFNGINPR
LGQYGYGSKYGGYRYVAYEYGAKHDA
>Mature_746_residues
MNPNPTGTSALADSEGDTDFVAVLDILIEGRWLIAAIALGCFVVGVAYAVLSKPVYQADILIQVEDSPDTSAAKSLLGDV
SSLFDVKSSAAAETQILASRLVVSRAVDNLKLFIDAKPKRFPVIGNWLARRSEGLSNPGLAGFGGYAWGQERINVATFDV
PRAMEGDTFELTMLDARRYRLAGGDLERNVEGAIGTLERFSAKGGAIVLRVDAVAAKPGATFVLVRHSRLRTIEDLQDNL
DVQERVKQSDVVVASLRDTDPDLVSSALNEIGRQYIAQNIQRKSAEAAQSLEFLNGQLPALKRQLTDSEARLTKLRDEHG
TVDLTEEAKLALAQSADAKTRLLELRQKRQEALSRFTPKHPSVIAIDQQIAALDGYRGAAEQQIKRLPDLQQQLVRLMLD
VKVNTDLYTALLNNMQQLQLVRAGKVGNVRLVDTAAVPEVPVKPKKALVALASLLLGVLAGCGTAVGRSMLFHGISDPNE
IERRLGLNVYATVPRSDQQRALTERAKRRERALSLLSVAHPDEPAVESLRSLRTALQFAMLDARNNVVVIAGPAPGVGKS
FVSANLAAVLTMAGKRVLLIDGDIRKGHLNDYLGLARGKGFSELIAGSAQPDEVLHRDVIVGLDFISTGAMPKHPAELLL
HPRLPELIGEFSKHYDVVLIDSPPVLAVADTGILAATAGTAFLVALAGSTKLGEIAESAKRLAQNGVRLSGVVFNGINPR
LGQYGYGSKYGGYRYVAYEYGAKHDA

Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor. May be involved in an ATP-dependent process in the pathway for EPS I production, possibly export of the trimeric repeat units across the in

COG id: COG3206

COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the etk/wzc family [H]

Homologues:

Organism=Escherichia coli, GI87082032, Length=746, Percent_Identity=35.656836461126, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787216, Length=732, Percent_Identity=32.3770491803279, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR005702
- InterPro:   IPR005700
- InterPro:   IPR003856 [H]

Pfam domain/function: PF01656 CbiA; PF02706 Wzz [H]

EC number: 2.7.1.112 [C]

Molecular weight: Translated: 80359; Mature: 80359

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNPTGTSALADSEGDTDFVAVLDILIEGRWLIAAIALGCFVVGVAYAVLSKPVYQADI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LIQVEDSPDTSAAKSLLGDVSSLFDVKSSAAAETQILASRLVVSRAVDNLKLFIDAKPKR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
FPVIGNWLARRSEGLSNPGLAGFGGYAWGQERINVATFDVPRAMEGDTFELTMLDARRYR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCHHHE
LAGGDLERNVEGAIGTLERFSAKGGAIVLRVDAVAAKPGATFVLVRHSRLRTIEDLQDNL
ECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCC
DVQERVKQSDVVVASLRDTDPDLVSSALNEIGRQYIAQNIQRKSAEAAQSLEFLNGQLPA
CHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LKRQLTDSEARLTKLRDEHGTVDLTEEAKLALAQSADAKTRLLELRQKRQEALSRFTPKH
HHHHHCCHHHHHHHHHHCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
PSVIAIDQQIAALDGYRGAAEQQIKRLPDLQQQLVRLMLDVKVNTDLYTALLNNMQQLQL
CCEEEECHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VRAGKVGNVRLVDTAAVPEVPVKPKKALVALASLLLGVLAGCGTAVGRSMLFHGISDPNE
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHH
IERRLGLNVYATVPRSDQQRALTERAKRRERALSLLSVAHPDEPAVESLRSLRTALQFAM
HHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LDARNNVVVIAGPAPGVGKSFVSANLAAVLTMAGKRVLLIDGDIRKGHLNDYLGLARGKG
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCC
FSELIAGSAQPDEVLHRDVIVGLDFISTGAMPKHPAELLLHPRLPELIGEFSKHYDVVLI
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCCHHHHHHHHHCCEEEEE
DSPPVLAVADTGILAATAGTAFLVALAGSTKLGEIAESAKRLAQNGVRLSGVVFNGINPR
CCCCEEEEECCCEEEECCHHEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
LGQYGYGSKYGGYRYVAYEYGAKHDA
CCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MNPNPTGTSALADSEGDTDFVAVLDILIEGRWLIAAIALGCFVVGVAYAVLSKPVYQADI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LIQVEDSPDTSAAKSLLGDVSSLFDVKSSAAAETQILASRLVVSRAVDNLKLFIDAKPKR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
FPVIGNWLARRSEGLSNPGLAGFGGYAWGQERINVATFDVPRAMEGDTFELTMLDARRYR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCHHHE
LAGGDLERNVEGAIGTLERFSAKGGAIVLRVDAVAAKPGATFVLVRHSRLRTIEDLQDNL
ECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCC
DVQERVKQSDVVVASLRDTDPDLVSSALNEIGRQYIAQNIQRKSAEAAQSLEFLNGQLPA
CHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LKRQLTDSEARLTKLRDEHGTVDLTEEAKLALAQSADAKTRLLELRQKRQEALSRFTPKH
HHHHHCCHHHHHHHHHHCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
PSVIAIDQQIAALDGYRGAAEQQIKRLPDLQQQLVRLMLDVKVNTDLYTALLNNMQQLQL
CCEEEECHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VRAGKVGNVRLVDTAAVPEVPVKPKKALVALASLLLGVLAGCGTAVGRSMLFHGISDPNE
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHH
IERRLGLNVYATVPRSDQQRALTERAKRRERALSLLSVAHPDEPAVESLRSLRTALQFAM
HHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LDARNNVVVIAGPAPGVGKSFVSANLAAVLTMAGKRVLLIDGDIRKGHLNDYLGLARGKG
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCC
FSELIAGSAQPDEVLHRDVIVGLDFISTGAMPKHPAELLLHPRLPELIGEFSKHYDVVLI
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCCHHHHHHHHHCCEEEEE
DSPPVLAVADTGILAATAGTAFLVALAGSTKLGEIAESAKRLAQNGVRLSGVVFNGINPR
CCCCEEEEECCCEEEECCHHEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
LGQYGYGSKYGGYRYVAYEYGAKHDA
CCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; a protein tyrosine [C]

Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]