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The map label for this gene is narK [H]

Identifier: 83721303

GI number: 83721303

Start: 2092064

End: 2093449

Strand: Reverse

Name: narK [H]

Synonym: BTH_I1855

Alternate gene names: 83721303

Gene position: 2093449-2092064 (Counterclockwise)

Preceding gene: 83719177

Following gene: 83720728

Centisome position: 54.96

GC content: 67.82

Gene sequence:

>1386_bases
ATGTCCACCTCTCTACTCGTGCGCTGGGACCCCGAAAACCCGGCATTCTGGCAAGCCAAGGGGCGCCCCGTCGCGTGGCG
CAATCTCGCGATCTCGATTCCCGCGCTGATGCTCGCGTTCGTCGTCTGGTCGCTCTGGAGCGTCGTCGTCGTCAACCTCG
ATCGCGCGGGCTTTCACTTCAGCAAGAACCAGCTGTTCTGGCTCACCGCGCTGCCCGCGCTATCCGGCGCGACGCTGCGC
ATCTTCTATTCGTTCCTCGTGCCGATCTTCGGCGGCCGCCGCTTCACCGCGATCTCGACCGCGACGCTGCTGATCCCCGC
GCTCGGCATGGGCTTCGCGCTGCGCGATCCCGGCACGGGCTATCCGACGCTCCTCATCCTCGCGCTGCTCTGCGGGTTCG
GCGGCGCGAACTTCAGCTCGTCGATGGCGAACATCAGCTTCTTCTTCCCGAAGGCGAAGAAGGGTCTCGCGACCGGCCTG
AACGCGGGCATCGGCAACCTCGGCGTGTCGGTCGTGCAGTTCGTCACGCCGCTCGTGATCTCGGCAGGCCTGTTCGGCGC
GCTCGCGGGCGATCCGCAGACCGCCGTCGCGCACGGCACGACGACGAGCCTGTGGCTGCAGAACGCGGGCTTCGTGTGGG
TGCCGTTCATTGTCGTCGCGACGCTCGCCGCGTGGTTCGGGATGAACGACATCGCCGACGCGAAGGCATCGTTCGCCGAG
CAGGCGGTGATCTTCCGCCGGCTGCACAACTGGCTGATGTGCTGGCTGTATGTCGGCACCTTCGGCTCGTTCATCGGCTT
TTCCGCGGGCTTCGCGCTCCTCACGAAGGCGCTCTTCCCGAACGTGAACCCGACCGCGTACGCGTTCATCGGCCCGCTCG
CGGGCGCGTTGATGCGCCCCGTCGGCGGCTGGGTGTCCGACCGGATCGGCGGCGCGCGCGTCACGTTCTGGACCTTCGCC
GCGATGATCGCGGCAGTCGGCGGCGTGATCGCGACGCTGCCGTCGGGCGGCGACGCGGGCAGCTTCGCAGGCTTCCTCGC
GATGTTCATCGTGTTGTTCGCGCTGACGGGCATCGGCAACGGTTCGACGTTCCGGATGATTCCCGTGATCTTCCTGACCG
AGCGGCAGCGCGCCGCGCACGGCCAGGGCGAAGCCGCGCAGAAGCAGGCGGTGCTCGACGCCGGCAAGGAATCGGCCGCC
GTGCTCGGCTTCTCGGGCGCGATCGGCGCGTACGGCGGCTTCTTCATCCCGAAGAGCTTCGGCACGTCGCTCGACATGAC
GGGCTCGGCCGTGCCGGCGCTCACCTGCTTCATCGTGTTCTACGTGTCGTGCGTCGCGATCACCTGGTGGTTCTACGCGC
GCCGCAACGCGTCGATGCCCTGCTGA

Upstream 100 bases:

>100_bases
GCTTCCGCGCCGAGCGCGCGGCGCACGACCAGCGCGTGCTCGCCGCCCGCTCCGCGTGAGCGTCGTCTTCGCCATCTTCG
TTCACTCAACGGATTCGATC

Downstream 100 bases:

>100_bases
CGGCAGGCCGAACACCGCGCGGGCCGGATGCGCCGCGCCGCGACACGCGGCCGCACCCGGCCCGCATTCCGCGAGGGCGC
GCCGCGCCCTCTAGTTCTTT

Product: nitrate/nitrite transporter NarK

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSTSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVVNLDRAGFHFSKNQLFWLTALPALSGATLR
IFYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLCGFGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVISAGLFGALAGDPQTAVAHGTTTSLWLQNAGFVWVPFIVVATLAAWFGMNDIADAKASFAE
QAVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIGPLAGALMRPVGGWVSDRIGGARVTFWTFA
AMIAAVGGVIATLPSGGDAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIFLTERQRAAHGQGEAAQKQAVLDAGKESAA
VLGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALTCFIVFYVSCVAITWWFYARRNASMPC

Sequences:

>Translated_461_residues
MSTSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVVNLDRAGFHFSKNQLFWLTALPALSGATLR
IFYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLCGFGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVISAGLFGALAGDPQTAVAHGTTTSLWLQNAGFVWVPFIVVATLAAWFGMNDIADAKASFAE
QAVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIGPLAGALMRPVGGWVSDRIGGARVTFWTFA
AMIAAVGGVIATLPSGGDAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIFLTERQRAAHGQGEAAQKQAVLDAGKESAA
VLGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALTCFIVFYVSCVAITWWFYARRNASMPC
>Mature_460_residues
STSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVVNLDRAGFHFSKNQLFWLTALPALSGATLRI
FYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLCGFGGANFSSSMANISFFFPKAKKGLATGLN
AGIGNLGVSVVQFVTPLVISAGLFGALAGDPQTAVAHGTTTSLWLQNAGFVWVPFIVVATLAAWFGMNDIADAKASFAEQ
AVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIGPLAGALMRPVGGWVSDRIGGARVTFWTFAA
MIAAVGGVIATLPSGGDAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIFLTERQRAAHGQGEAAQKQAVLDAGKESAAV
LGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALTCFIVFYVSCVAITWWFYARRNASMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=456, Percent_Identity=53.9473684210526, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=54.1125541125541, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48885; Mature: 48753

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVVNLDRAGFHF
CCCCEEEEECCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
SKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCEEECCCCCC
YPTLLILALLCGFGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVI
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SAGLFGALAGDPQTAVAHGTTTSLWLQNAGFVWVPFIVVATLAAWFGMNDIADAKASFAE
HHHHHHHHCCCCCCEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QAVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIGPLAGALMRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VGGWVSDRIGGARVTFWTFAAMIAAVGGVIATLPSGGDAGSFAGFLAMFIVLFALTGIGN
HCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAHGQGEAAQKQAVLDAGKESAAVLGFSGAIGAYGGFFIPKSF
CCCEEEEEEEEEECHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCEECCCCC
GTSLDMTGSAVPALTCFIVFYVSCVAITWWFYARRNASMPC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVVNLDRAGFHF
CCCEEEEECCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
SKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCEEECCCCCC
YPTLLILALLCGFGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVI
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SAGLFGALAGDPQTAVAHGTTTSLWLQNAGFVWVPFIVVATLAAWFGMNDIADAKASFAE
HHHHHHHHCCCCCCEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QAVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIGPLAGALMRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VGGWVSDRIGGARVTFWTFAAMIAAVGGVIATLPSGGDAGSFAGFLAMFIVLFALTGIGN
HCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAHGQGEAAQKQAVLDAGKESAAVLGFSGAIGAYGGFFIPKSF
CCCEEEEEEEEEECHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCEECCCCC
GTSLDMTGSAVPALTCFIVFYVSCVAITWWFYARRNASMPC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]