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The map label for this gene is ynfL [H]

Identifier: 83720744

GI number: 83720744

Start: 2057818

End: 2058720

Strand: Reverse

Name: ynfL [H]

Synonym: BTH_I1832

Alternate gene names: 83720744

Gene position: 2058720-2057818 (Counterclockwise)

Preceding gene: 83718889

Following gene: 83721528

Centisome position: 54.05

GC content: 67.22

Gene sequence:

>903_bases
ATGGACCTCAAGCAATTGCGCTACTTCGTCGCCGTCGCGGAGGAACTGCATTTCGGCCGCGCGGCGAAACGGCTGTTCAT
CTCGCAGCCGGCGTTGAGCTTCGACATCCGCAAGTTCGAGGACGAACTCGGCGTGCAGCTGTTCGCGCGCACGAGCAAGT
CGGTCGCGCTGACGAATGCGGGCGAAGTGCTGCTCGGCGAGGCGCGCCGGCTGCTGCTGCAGGCGCAGGAAGCGCAACGA
CTGACGATTCGGTCGGCGTCGGGGCTCGCGGGGCGACTCAAGGTCGGCTTCGTCAATTCGATGCTGTATCGCGGGTTGCC
GTCCGCGGTGCGGCGCTTCGAGGCGGATTATCCGAATGTCGAAGTCGTGCTGAAGGAAATGAACACGCACGAGCAGGTGC
AGGCGATCCTGCGCGGGCAGATCGACGTCGGCTACGCGCATTGGGGCACGTTTCCGCCCGAGATCTCGGCCGATACGATT
TTCTCCGAACCGTTCCTCTGCTGCCTGCCGGCCGCGCACCGGCTCGCGCGACGCAGGCGCGTCGACCTCGCGGCGCTCGC
GCATGAGCCGTTCATCCTGTTCCCGCGCGATGCGGCGCCCCACTACCACGATCTCATCATCGCGCAGTGCGTGAGCGCCG
GCTTCAGCCCGCAGATCCGGCACGAGGCGCGCCTGTGGCAGACGGTGCTGACGATGGTCGAGTTCGAGATGGGGGTGGCG
CTCGTGCCGAGCGTGCTGCGCCAGGTGCGCAGCGAGCGGCTGCGCTACCTGCCGCTCGCCGGCGACGCACTAGAGTCGCG
CACGTTGCGGTTGCGGCGCTCGGATGAACGGGAGCCCGTTGCAGAGCGTTTCGGCGAATATCTGGACGACGGGATCCGGC
GGCTTCGCGGCGCGGCGAAGTGA

Upstream 100 bases:

>100_bases
CCTGGTCCGACGAGCGTAGAACCGCGACCGATAATCGTCCAATACAGGATTTATCCAGTACGATAAGCTGCATTTATCGA
TCGCCCCGAACGGAGACGCG

Downstream 100 bases:

>100_bases
CGGCGCGCGAGCGGACGCTCAAAAAGTTCTGGATAAAAGTGCGCGATTGCCGGTAGAATCACGCCCCTGGTCGCGCGGTG
CGTGACCGGCACCCGTCAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDLKQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDELGVQLFARTSKSVALTNAGEVLLGEARRLLLQAQEAQR
LTIRSASGLAGRLKVGFVNSMLYRGLPSAVRRFEADYPNVEVVLKEMNTHEQVQAILRGQIDVGYAHWGTFPPEISADTI
FSEPFLCCLPAAHRLARRRRVDLAALAHEPFILFPRDAAPHYHDLIIAQCVSAGFSPQIRHEARLWQTVLTMVEFEMGVA
LVPSVLRQVRSERLRYLPLAGDALESRTLRLRRSDEREPVAERFGEYLDDGIRRLRGAAK

Sequences:

>Translated_300_residues
MDLKQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDELGVQLFARTSKSVALTNAGEVLLGEARRLLLQAQEAQR
LTIRSASGLAGRLKVGFVNSMLYRGLPSAVRRFEADYPNVEVVLKEMNTHEQVQAILRGQIDVGYAHWGTFPPEISADTI
FSEPFLCCLPAAHRLARRRRVDLAALAHEPFILFPRDAAPHYHDLIIAQCVSAGFSPQIRHEARLWQTVLTMVEFEMGVA
LVPSVLRQVRSERLRYLPLAGDALESRTLRLRRSDEREPVAERFGEYLDDGIRRLRGAAK
>Mature_300_residues
MDLKQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDELGVQLFARTSKSVALTNAGEVLLGEARRLLLQAQEAQR
LTIRSASGLAGRLKVGFVNSMLYRGLPSAVRRFEADYPNVEVVLKEMNTHEQVQAILRGQIDVGYAHWGTFPPEISADTI
FSEPFLCCLPAAHRLARRRRVDLAALAHEPFILFPRDAAPHYHDLIIAQCVSAGFSPQIRHEARLWQTVLTMVEFEMGVA
LVPSVLRQVRSERLRYLPLAGDALESRTLRLRRSDEREPVAERFGEYLDDGIRRLRGAAK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=295, Percent_Identity=35.9322033898305, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI2367136, Length=261, Percent_Identity=39.0804597701149, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788887, Length=289, Percent_Identity=33.2179930795848, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1790399, Length=262, Percent_Identity=30.9160305343511, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI157672245, Length=285, Percent_Identity=30.1754385964912, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI145693105, Length=286, Percent_Identity=29.3706293706294, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=29.2181069958848, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790208, Length=281, Percent_Identity=28.4697508896797, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787601, Length=175, Percent_Identity=31.4285714285714, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788297, Length=153, Percent_Identity=32.0261437908497, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=24.3027888446215, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789173, Length=174, Percent_Identity=32.183908045977, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788481, Length=199, Percent_Identity=28.643216080402, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788296, Length=197, Percent_Identity=26.9035532994924, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI48994962, Length=80, Percent_Identity=45, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081904, Length=212, Percent_Identity=28.3018867924528, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790262, Length=291, Percent_Identity=25.085910652921, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=144, Percent_Identity=29.8611111111111, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786713, Length=114, Percent_Identity=36.8421052631579, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=23.265306122449, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=210, Percent_Identity=25.7142857142857, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=30.327868852459, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33975; Mature: 33975

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDELGVQLFARTSKSVALTNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GEVLLGEARRLLLQAQEAQRLTIRSASGLAGRLKVGFVNSMLYRGLPSAVRRFEADYPNV
CHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCH
EVVLKEMNTHEQVQAILRGQIDVGYAHWGTFPPEISADTIFSEPFLCCLPAAHRLARRRR
HHHHHHHCCHHHHHHHHHCCCEECCHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
VDLAALAHEPFILFPRDAAPHYHDLIIAQCVSAGFSPQIRHEARLWQTVLTMVEFEMGVA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVPSVLRQVRSERLRYLPLAGDALESRTLRLRRSDEREPVAERFGEYLDDGIRRLRGAAK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLKQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDELGVQLFARTSKSVALTNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GEVLLGEARRLLLQAQEAQRLTIRSASGLAGRLKVGFVNSMLYRGLPSAVRRFEADYPNV
CHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCH
EVVLKEMNTHEQVQAILRGQIDVGYAHWGTFPPEISADTIFSEPFLCCLPAAHRLARRRR
HHHHHHHCCHHHHHHHHHCCCEECCHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
VDLAALAHEPFILFPRDAAPHYHDLIIAQCVSAGFSPQIRHEARLWQTVLTMVEFEMGVA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVPSVLRQVRSERLRYLPLAGDALESRTLRLRRSDEREPVAERFGEYLDDGIRRLRGAAK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]