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The map label for this gene is nuoB [H]

Identifier: 83720537

GI number: 83720537

Start: 1204004

End: 1204525

Strand: Direct

Name: nuoB [H]

Synonym: BTH_I1062

Alternate gene names: 83720537

Gene position: 1204004-1204525 (Clockwise)

Preceding gene: 83720995

Following gene: 83719353

Centisome position: 31.61

GC content: 66.09

Gene sequence:

>522_bases
ATGGGCGGCCACGCGTCGCCGCCTGTCTGGAGTGGAAAGCAAATGAGTATCGAAGGGGTCTTGAAGGAAGGGTTTGTCAC
CACGACGGCTGACAAGCTGATCAACTGGACGCGTACGGGCTCGCTGTGGCCGATGACGTTCGGGCTCGCGTGCTGCGCAG
TGGAAATGATGCATGCGGGCGCCGCCCGCTACGACCTCGACCGGTTCGGCGTCGTGTTCCGTCCGAGCCCGCGCCAGTCC
GACGTGATGATTGTCGCGGGCACGCTCTGCAACAAGATGGCGCCCGCGCTGCGCCGCGTCTACGACCAGATGGCCGAGCC
GCGCTGGGTGATCTCGATGGGGTCGTGCGCGAACGGCGGCGGCTACTACCACTACTCGTACTCGGTGGTGCGCGGCTGCG
ATCGGATCGTGCCCGTCGACGTCTACGTGCCCGGCTGCCCGCCGACCGCCGAGGCGCTTGTCTACGGTGTGATCCAGCTG
CAGGCGAAGATCCGCCGCACGAGCACCATCGCCCGTCAATAA

Upstream 100 bases:

>100_bases
TCGCAATGATGATTTTCCTGCTCGAATTCCTGCTCGGCTTTGCCTATATCTGGAAGAAGGGCGGCCTCGACTGGGAGTGA
CGGGCTAATCGCCGGTTTGC

Downstream 100 bases:

>100_bases
AGCATCGAGCACCCCACAACATGGCAAGCAAAATCGAGACCCTCAAGGCGAACCTCGAAGCCGCGCTCGGCGCGCGCGCG
GTGAGCCTCGTCGAAGCGGT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MGGHASPPVWSGKQMSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQS
DVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQL
QAKIRRTSTIARQ

Sequences:

>Translated_173_residues
MGGHASPPVWSGKQMSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQS
DVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQL
QAKIRRTSTIARQ
>Mature_172_residues
GGHASPPVWSGKQMSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSD
VMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQ
AKIRRTSTIARQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=78.7671232876712, Blast_Score=256, Evalue=9e-69,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=51.006711409396, Blast_Score=164, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=138, Percent_Identity=35.5072463768116, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1788834, Length=109, Percent_Identity=37.6146788990826, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=71.0344827586207, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=80.7142857142857, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=80.7142857142857, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24651058, Length=165, Percent_Identity=69.6969696969697, Blast_Score=252, Evalue=9e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18954; Mature: 18823

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGHASPPVWSGKQMSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAG
CCCCCCCCCCCCCEEEHHHHHHCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
AARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGG
CCCCCHHHCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
GYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTSTIARQ
CEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GGHASPPVWSGKQMSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAG
CCCCCCCCCCCCEEEHHHHHHCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
AARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGG
CCCCCHHHCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
GYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTSTIARQ
CEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA